FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34211-1.3410000003589a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34211-1.3410000003589a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696177
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT340220.9204645773186727TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG92900.042.0840843
CGGAAGA94850.041.1159364
GATCGGA98550.039.9761851
ATCGGAA98000.039.8939932
ATGCCGT50200.038.22700548-49
GCCGTCT50450.038.0375750-51
CGTCTTC51250.037.7216152-53
CGTATGC50450.037.61388846-47
TGCCGTC50550.037.53948250-51
TCTCGTA50200.037.28079242-43
TATGCCG50950.037.24476248-49
CTCGTAT50500.037.05932644-45
AATCTCG50950.036.7778740-41
TCGTATG52450.036.7682744-45
AGAGCAC106300.036.5501488
GAGCACA106300.036.5054669
CCGTCTT52550.036.2009152-53
ATCTCGT51800.035.94586642-43
GAAGAGC110700.035.18326
GTATGCC55450.034.6505146-47
Produced by FastQC (version 0.11.2)