##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34139-1.341000000356ec.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718311 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.682827682336566 33.0 33.0 33.0 33.0 33.0 2 32.7054185485031 33.0 33.0 33.0 33.0 33.0 3 32.74194674560784 33.0 33.0 33.0 33.0 33.0 4 36.66518768455854 37.0 37.0 37.0 37.0 37.0 5 36.66824583353413 37.0 37.0 37.0 37.0 37.0 6 36.55385899553068 37.0 37.0 37.0 37.0 37.0 7 36.43148484481724 37.0 37.0 37.0 37.0 37.0 8 36.38501003012532 37.0 37.0 37.0 37.0 37.0 9 36.428576421167406 37.0 37.0 37.0 37.0 37.0 10-11 36.345347165942385 37.0 37.0 37.0 37.0 37.0 12-13 36.355359265367355 37.0 37.0 37.0 37.0 37.0 14-15 38.218019939587485 40.0 37.0 40.0 37.0 40.0 16-17 38.22060371311451 40.0 37.0 40.0 37.0 40.0 18-19 38.173335390983596 40.0 37.0 40.0 37.0 40.0 20-21 38.17231104167257 40.0 37.0 40.0 37.0 40.0 22-23 37.96967528733835 40.0 37.0 40.0 33.0 40.0 24-25 38.04371574849235 40.0 37.0 40.0 35.0 40.0 26-27 37.97400168707701 40.0 37.0 40.0 35.0 40.0 28-29 37.773491885218434 40.0 37.0 40.0 33.0 40.0 30-31 37.71656830289102 40.0 37.0 40.0 33.0 40.0 32-33 37.61988142637101 40.0 37.0 40.0 33.0 40.0 34-35 37.506670134506315 40.0 37.0 40.0 33.0 40.0 36-37 37.460590050218684 40.0 37.0 40.0 33.0 40.0 38-39 37.405424360935896 40.0 37.0 40.0 33.0 40.0 40-41 37.354102786620075 40.0 37.0 40.0 33.0 40.0 42-43 36.939297232730176 37.0 37.0 40.0 33.0 40.0 44-45 36.760129175800714 37.0 37.0 40.0 33.0 40.0 46-47 36.965792177569085 37.0 37.0 40.0 33.0 40.0 48-49 36.72042014324336 37.0 37.0 40.0 33.0 40.0 50-51 36.64022861990405 37.0 37.0 40.0 33.0 40.0 52-53 36.545690688078004 37.0 37.0 40.0 33.0 40.0 54-55 36.261248069113506 37.0 37.0 40.0 33.0 40.0 56-57 36.13274731993506 37.0 37.0 40.0 33.0 40.0 58-59 35.96578114130429 37.0 37.0 40.0 33.0 40.0 60-61 35.67634645189605 37.0 35.0 40.0 27.0 40.0 62-63 35.31831806588724 37.0 33.0 38.5 27.0 40.0 64-65 35.03462738443099 37.0 33.0 37.0 27.0 40.0 66-67 34.48614580892326 37.0 33.0 37.0 27.0 40.0 68-69 34.26938970559292 37.0 33.0 37.0 27.0 40.0 70-71 33.79098694005211 37.0 33.0 37.0 27.0 40.0 72-73 32.86946986566291 37.0 33.0 37.0 22.0 37.0 74-75 32.18069731535501 37.0 33.0 37.0 22.0 37.0 76-77 29.560871989996727 33.0 27.0 35.0 18.5 37.0 78-79 31.71808045510613 33.0 33.0 37.0 18.5 37.0 80-81 32.04201855490413 37.0 33.0 37.0 15.0 37.0 82-83 31.964633553703017 37.0 33.0 37.0 15.0 37.0 84-85 31.76214826044555 37.0 33.0 37.0 15.0 37.0 86-87 31.58113733123252 33.0 33.0 37.0 15.0 37.0 88-89 31.438060435321788 33.0 33.0 37.0 15.0 37.0 90-91 31.287493042554733 33.0 33.0 37.0 15.0 37.0 92-93 31.162977672532687 33.0 33.0 37.0 15.0 37.0 94-95 30.98587118986753 33.0 33.0 37.0 15.0 37.0 96-97 30.827243093229583 33.0 33.0 37.0 15.0 37.0 98-99 30.69340134370203 33.0 33.0 37.0 15.0 37.0 100-101 29.156170688342872 33.0 27.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16139984945871788 2306 2 0.165081869385439 2306 3 0.10877050116548759 2306 4 0.10562542322922042 2306 5 0.1032498726398785 2306 6 0.09151785503993892 2306 7 0.09200887018584325 2306 8 0.18385307975069765 2306 9 0.18049716452512854 2306 10-11 0.21513152278178183 2306 12-13 0.20992566015416259 2306 14-15 0.3442103137004153 2306 16-17 0.36155618709639015 2306 18-19 0.35787164485880396 2306 20-21 0.30264801494102045 2306 22-23 0.31107743634276375 2306 24-25 0.33351734795571275 2306 26-27 0.3799666410436373 2306 28-29 0.40585754943876395 2306 30-31 0.4452489062488283 2306 32-33 0.4726008942671882 2306 34-35 0.4374040254026852 2306 36-37 0.4594126057879322 2306 38-39 0.40887662719458007 2306 40-41 0.4389360980852146 2306 42-43 0.5138406303101064 2306 44-45 0.46126239951077963 2306 46-47 0.41694557192328574 2306 48-49 0.4324478485457135 2306 50-51 0.31378712914979445 2306 52-53 0.4827956549352592 2306 54-55 0.35504846124992184 2306 56-57 0.5062830241224745 2306 58-59 0.45642471199685986 2306 60-61 0.5153067150312296 2306 62-63 0.3619840937399772 2306 64-65 0.3830081317981566 2306 66-67 0.43945016403289827 2306 68-69 0.2828170903132232 2306 70-71 0.3715830131562612 2306 72-73 0.23758755066798898 2306 74-75 0.3597994851479882 2306 76-77 -0.0029059837053146964 2306 78-79 0.36864476043657746 2306 80-81 0.43216744332877965 2306 82-83 0.516937076724048 2306 84-85 0.5491149663558765 2306 86-87 0.4444558367618576 2306 88-89 0.561123543431691 2306 90-91 0.49011690848373846 2306 92-93 0.4771880265629349 2306 94-95 0.2796322785029304 2306 96-97 0.3904629945843716 2306 98-99 0.44143996782429085 2306 100-101 0.4659812606088778 2307 1 0.13236197869576927 2307 2 0.08973860372441322 2307 3 0.14067078190930715 2307 4 0.17328518580589503 2307 5 0.16216182382311928 2307 6 0.13293669252147566 2307 7 0.22317996515972993 2307 8 0.15115992809337797 2307 9 0.19123125897804272 2307 10-11 0.18091695311726141 2307 12-13 0.20983225375626802 2307 14-15 0.40535249945118323 2307 16-17 0.32010940753001904 2307 18-19 0.23773790149533625 2307 20-21 0.23133719129504016 2307 22-23 0.27449805390617854 2307 24-25 0.35601813945803684 2307 26-27 0.34114459266299946 2307 28-29 0.40331459161967587 2307 30-31 0.4075316536879612 2307 32-33 0.3778645671846661 2307 34-35 0.37329245140337974 2307 36-37 0.34904844172843497 2307 38-39 0.34678657749644515 2307 40-41 0.4161062742777375 2307 42-43 0.2909541467975387 2307 44-45 0.32717700211275513 2307 46-47 0.2754189122090551 2307 48-49 0.15573293445222447 2307 50-51 0.2666839592172039 2307 52-53 0.21950639938539496 2307 54-55 0.2699843153470738 2307 56-57 0.31445490923009345 2307 58-59 0.15215924439918638 2307 60-61 0.1689628627004751 2307 62-63 0.14080835011556303 2307 64-65 0.11505277617867904 2307 66-67 0.22830403217545125 2307 68-69 0.3020693008172586 2307 70-71 0.21239490293533692 2307 72-73 0.13877110895220568 2307 74-75 0.33260659032974615 2307 76-77 0.0875517829169219 2307 78-79 0.47782178506302486 2307 80-81 0.5034178208320412 2307 82-83 0.6347170701000593 2307 84-85 0.8177132180253288 2307 86-87 0.5977252431670443 2307 88-89 0.41792925447449036 2307 90-91 0.49693306644479307 2307 92-93 0.5455296900590305 2307 94-95 0.4935794618039182 2307 96-97 0.6980234612559642 2307 98-99 0.4598647504690838 2307 100-101 0.3168769355847232 2201 1 0.11841697878664803 2201 2 0.1279086932321576 2201 3 0.10961694672452182 2201 4 0.13510972616369799 2201 5 0.09964519706068131 2201 6 0.12594932142535242 2201 7 0.17056049619167624 2201 8 0.10938152132138868 2201 9 0.1298089003819456 2201 10-11 0.13021296979937347 2201 12-13 0.1112942108976327 2201 14-15 0.2562649679119744 2201 16-17 0.18123958950322105 2201 18-19 0.24229074279216434 2201 20-21 0.21088627349775635 2201 22-23 0.273004123810459 2201 24-25 0.1822645476642677 2201 26-27 0.19478798103607886 2201 28-29 0.1777253639441554 2201 30-31 0.27474186057859384 2201 32-33 0.24462967254670787 2201 34-35 0.12599053087807022 2201 36-37 0.09355314183534347 2201 38-39 0.2678534497153322 2201 40-41 0.28776894225967453 2201 42-43 0.2230184347424924 2201 44-45 0.12108714571840551 2201 46-47 0.16337002415735924 2201 48-49 0.1950683431359792 2201 50-51 0.2777003769574833 2201 52-53 0.20772432439848387 2201 54-55 0.19765480059242435 2201 56-57 0.2652039603686447 2201 58-59 0.3099530031463118 2201 60-61 0.015465831145561992 2201 62-63 0.1122340712420069 2201 64-65 0.14956848919208454 2201 66-67 0.3417923228038191 2201 68-69 0.28694204515424104 2201 70-71 0.19138879585320723 2201 72-73 -0.021674876583126945 2201 74-75 0.16581868255065046 2201 76-77 0.0839182673294836 2201 78-79 0.30594670760915577 2201 80-81 0.2521044098713503 2201 82-83 0.2272409754601199 2201 84-85 0.31387852893958623 2201 86-87 0.2197605158624718 2201 88-89 0.13827268696529416 2201 90-91 0.19380694594558534 2201 92-93 0.09577865775395367 2201 94-95 0.18067205448830848 2201 96-97 0.27713091186312155 2201 98-99 0.14988468606052052 2201 100-101 0.1317330820753817 1101 1 0.04671750420312293 1101 2 0.07832701065605363 1101 3 0.025380006310022907 1101 4 0.10882646846847877 1101 5 0.057030699032168286 1101 6 0.04507003534371279 1101 7 -0.02006037691525364 1101 8 -0.031237562091540383 1101 9 0.10432535332147097 1101 10-11 0.11261641580807691 1101 12-13 0.05557321854056596 1101 14-15 0.13432953630371713 1101 16-17 0.11432443795381886 1101 18-19 0.06037041996904691 1101 20-21 0.061295736646066246 1101 22-23 0.13404003637543127 1101 24-25 0.1500288619194592 1101 26-27 0.10885770751431778 1101 28-29 0.15983435819264002 1101 30-31 0.17218014910363877 1101 32-33 0.20359877112493052 1101 34-35 0.121627445294898 1101 36-37 -0.002517820473698862 1101 38-39 -0.05651615412176625 1101 40-41 -0.08301392641258332 1101 42-43 0.006523955477511834 1101 44-45 -0.06171653238534702 1101 46-47 0.12483386197717294 1101 48-49 0.03769037671818154 1101 50-51 0.04418072705930598 1101 52-53 0.14263560267329467 1101 54-55 -0.06637894361696794 1101 56-57 -0.050502865147493026 1101 58-59 0.018910421616325834 1101 60-61 0.05306598336375146 1101 62-63 -0.08434940291198956 1101 64-65 -0.18470377413030548 1101 66-67 -0.10572377031301272 1101 68-69 -0.12049827797029877 1101 70-71 -0.22982152710178383 1101 72-73 -0.3971503229233946 1101 74-75 -0.1956635231812669 1101 76-77 -0.26869072974483643 1101 78-79 -0.007527050767084376 1101 80-81 0.03894516666700554 1101 82-83 0.16036350167913582 1101 84-85 0.21981560329854588 1101 86-87 0.1239920118394302 1101 88-89 -0.042632785165402254 1101 90-91 0.09086817420315896 1101 92-93 6.895780899647264E-4 1101 94-95 0.02335267700039978 1101 96-97 0.027748638876385456 1101 98-99 0.019996231093927008 1101 100-101 0.008133800213645515 2304 1 0.07695933528385979 2304 2 0.10761342772404703 2304 3 0.050179343475370786 2304 4 0.12085070336051018 2304 5 0.11311790321631321 2304 6 0.08986315843417714 2304 7 0.013928023478307239 2304 8 0.04459227582467662 2304 9 0.11421349997363706 2304 10-11 0.06726273137951466 2304 12-13 0.060930268926362885 2304 14-15 0.12562206387868002 2304 16-17 0.13980214496018561 2304 18-19 0.18286666563214737 2304 20-21 0.0461359831377095 2304 22-23 0.05260587967717356 2304 24-25 0.19430673463449466 2304 26-27 0.11021962559210152 2304 28-29 0.05731377406960547 2304 30-31 0.06245512191706126 2304 32-33 0.09040033500044586 2304 34-35 0.03826951458182748 2304 36-37 0.1683744171110959 2304 38-39 0.04724077198252985 2304 40-41 0.1133107072159234 2304 42-43 -0.04143446200475154 2304 44-45 0.09659608736505731 2304 46-47 -0.001974692732581218 2304 48-49 -0.014344732804893567 2304 50-51 0.01213293046669861 2304 52-53 0.017363736897316073 2304 54-55 -0.07252828854991122 2304 56-57 -0.059722998750103784 2304 58-59 -0.001280143346647833 2304 60-61 0.033388079578337226 2304 62-63 0.06961336442268617 2304 64-65 -0.04772485020094308 2304 66-67 -0.17274515708739813 2304 68-69 -0.22095268219587183 2304 70-71 -0.1570003371064459 2304 72-73 -0.36822121516097894 2304 74-75 -0.526115079289184 2304 76-77 -0.7542999680028224 2304 78-79 -0.48280409493879617 2304 80-81 -0.19096265788894584 2304 82-83 0.0075244462185182215 2304 84-85 0.01667879008328299 2304 86-87 -0.11948191493805638 2304 88-89 -0.04083329264818758 2304 90-91 -0.19094788918040706 2304 92-93 -0.2831538272230709 2304 94-95 -0.1251753640124953 2304 96-97 -0.0684853728267889 2304 98-99 -0.13317812549040298 2304 100-101 0.07057748516004736 2202 1 0.01467299623595153 2202 2 0.07429503238216029 2202 3 0.06505122636151839 2202 4 0.05867814731126231 2202 5 0.04418892541988839 2202 6 0.04458896683377844 2202 7 -0.0258270570279322 2202 8 0.05973717434168435 2202 9 0.07796153737582046 2202 10-11 0.08493771246864412 2202 12-13 0.11240595178326629 2202 14-15 0.03167926049442826 2202 16-17 -0.034435540731756475 2202 18-19 0.036963648157865236 2202 20-21 -0.04313472505021565 2202 22-23 -0.0571643613012327 2202 24-25 -0.01906721732238026 2202 26-27 0.20268515697141964 2202 28-29 0.060295174681108676 2202 30-31 0.08274091429804997 2202 32-33 0.0169926613224618 2202 34-35 -0.09547588483463443 2202 36-37 -0.037546430604024295 2202 38-39 0.060111917503988366 2202 40-41 0.038833854746066265 2202 42-43 -0.08317772560626935 2202 44-45 -0.047381836190574234 2202 46-47 -0.12706924704011868 2202 48-49 -0.2780949950143139 2202 50-51 -0.07249349261535798 2202 52-53 -0.030999917380356123 2202 54-55 -0.04175539051600197 2202 56-57 0.031064392313801648 2202 58-59 -0.06516294050143756 2202 60-61 -0.1442710418135036 2202 62-63 -0.3171431526733599 2202 64-65 -0.3237948235668213 2202 66-67 -0.23016488922059608 2202 68-69 -0.2817288538747107 2202 70-71 -0.135384109998121 2202 72-73 -0.3692560683554831 2202 74-75 -0.27877308827740777 2202 76-77 -0.6468732436182982 2202 78-79 -0.25163089051715204 2202 80-81 -0.021873811801114584 2202 82-83 0.11690407461214392 2202 84-85 0.17814814813104718 2202 86-87 0.010624017669353947 2202 88-89 0.03790867420854127 2202 90-91 0.015620377440495048 2202 92-93 0.053507182076625526 2202 94-95 -0.037345536339710605 2202 96-97 0.08028097156065783 2202 98-99 -0.09700816293073089 2202 100-101 -0.04713770137414386 1102 1 -6.93070564352638E-5 1102 2 0.006024366378888146 1102 3 0.032741185928813366 1102 4 -0.03956282670071687 1102 5 -0.027632874661833284 1102 6 -0.1233949876152991 1102 7 -0.008261806164099994 1102 8 -0.0029933363950860326 1102 9 -0.015652914852125832 1102 10-11 -0.019871711513744117 1102 12-13 -0.045159992312953534 1102 14-15 -0.08843312212772503 1102 16-17 -0.07258094520067715 1102 18-19 -0.08265645172023994 1102 20-21 -0.09666008960416406 1102 22-23 -0.10387103946911935 1102 24-25 -0.07590349777085947 1102 26-27 -0.12937505655000336 1102 28-29 -0.08221863219721826 1102 30-31 -0.14752909240319667 1102 32-33 -0.06819989494124457 1102 34-35 -0.02922407533723259 1102 36-37 -0.06363310161729174 1102 38-39 -0.0817670113613076 1102 40-41 -0.15731127593993222 1102 42-43 -0.14028954398991544 1102 44-45 -0.17017574112764322 1102 46-47 -0.2695875877167495 1102 48-49 -0.13570148004239257 1102 50-51 -0.2097478009668805 1102 52-53 -0.09370015404462606 1102 54-55 -0.22766912783708904 1102 56-57 -0.23792461746662497 1102 58-59 -0.2508709018804893 1102 60-61 -0.26795598436734736 1102 62-63 -0.17979239141937597 1102 64-65 -0.19135628651530823 1102 66-67 -0.20406695683737297 1102 68-69 -0.18855439945883035 1102 70-71 -0.2496189350096003 1102 72-73 -0.3393548642079125 1102 74-75 -0.4372759198538212 1102 76-77 -0.5606042027623417 1102 78-79 -0.31088595235669914 1102 80-81 -0.2293597270448231 1102 82-83 -0.1301374026221893 1102 84-85 -0.1560911404779759 1102 86-87 -0.17946863854266581 1102 88-89 -0.17154373948329393 1102 90-91 -0.15833129870473428 1102 92-93 -0.14781671627619986 1102 94-95 -0.3203988271898872 1102 96-97 -0.189396151799901 1102 98-99 -0.18210141438659022 1102 100-101 -0.3004192938313075 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0.2929320580765342 1213 78-79 -0.25418795045075626 1213 80-81 -0.33455915084659793 1213 82-83 -0.2291872743748371 1213 84-85 -0.15147735085565373 1213 86-87 -0.22668380648001119 1213 88-89 -0.3150322791813025 1213 90-91 -0.18764294869365372 1213 92-93 -0.09409164557457572 1213 94-95 0.11745284743534157 1213 96-97 -0.03231620535500568 1213 98-99 0.016850135907812813 1213 100-101 0.13161520426804074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 10.0 10 10.0 11 13.0 12 33.0 13 58.0 14 242.0 15 699.0 16 1455.0 17 2386.0 18 3032.0 19 3816.0 20 5216.0 21 6924.0 22 9535.0 23 12445.0 24 16503.0 25 21911.0 26 29132.0 27 41740.0 28 68014.0 29 109025.0 30 93334.0 31 64817.0 32 79992.0 33 112287.0 34 171833.0 35 294550.0 36 549332.0 37 802368.0 38 217129.0 39 469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.151946584882566 24.520787078623123 18.344093041695007 14.983173294799307 2 29.44471769418584 28.751603477306315 27.514879644014243 14.288799184493605 3 35.76785894947897 18.448665005012554 28.870915228048233 16.912560817460246 4 29.8036892875275 18.463213815329603 26.885774831318475 24.84732206582442 5 38.1861383778383 16.55752413907018 27.497184832787713 17.75915265030381 6 35.06946041126273 17.403527410954815 29.661653872570138 17.865358305212318 7 32.0249228289184 25.20425367075364 23.001194491726665 19.769629008601296 8 28.638886426166838 31.5033121670037 20.87936222161482 18.978439185214643 9 34.6934548695863 22.1393725736312 23.39426945629106 19.772903100491448 10-11 26.371393854492737 30.06512131981955 27.243994524541158 16.31949030114656 12-13 22.49557215777962 29.248369529529462 26.915284328261986 21.34077398442893 14-15 22.37702014228688 29.495116636764518 27.056488385618866 21.07137483532973 16-17 25.917513838099072 25.592183085780672 27.347446143477722 21.142856932642538 18-19 21.144453302068822 25.63023509819149 31.45131296602927 21.773998633710416 20-21 24.672894308267153 25.793571817205613 31.31856509428097 18.214968780246263 22-23 20.492251514776395 33.578804884145654 27.424051490901224 18.504892110176726 24-25 20.66675998446829 25.168105397858486 31.640013765908552 22.525120851764672 26-27 20.679697797639783 25.282739907243872 27.739338876236015 26.29822341888033 28-29 24.159560844951148 30.011779373294665 27.264135707797966 18.564524073956218 30-31 19.703595357558427 27.0891005480977 30.894423044309498 22.312881050034377 32-33 19.369582803439343 31.256412529692152 27.32509267703364 22.048911989834867 34-35 19.65670962594052 30.949144523934162 31.590002026993968 17.804143823131348 36-37 19.50893404029193 31.180151939936234 31.771180707431927 17.539733312339905 38-39 19.73344624751935 27.08410089657518 31.311466755644226 21.87098610026125 40-41 23.720814137896657 26.520401823043795 27.283541140068223 22.475242898991322 42-43 19.935540856068346 30.348017574148066 31.023749673970347 18.692691895813248 44-45 19.45679384763667 26.470576333413653 31.509046998037384 22.563582820912295 46-47 23.445183515010783 26.301052982420124 27.690682229454737 22.56308127311435 48-49 19.471783406990035 30.956361117354938 30.951100362329903 18.620755113325124 50-51 23.44091982709005 26.8878618147746 30.413879148811564 19.25733920932379 52-53 19.432626748657736 26.78093937545054 26.720809075352705 27.065624800539023 54-55 23.70487493612621 26.481310960614014 30.88793124696726 18.92588285629252 56-57 19.85817481422946 26.53178723978007 30.478582285261364 23.13145566072911 58-59 19.769130970550165 26.95370350102063 30.600005996693724 22.677159531735477 60-61 23.267257163269235 26.97618748935447 30.679673190039132 19.076882157337167 62-63 19.423865739122757 27.04378487946466 30.365873398349695 23.16647598306288 64-65 23.403775510373006 26.933781508648018 30.47809801891379 19.184344962065186 66-67 19.96301455816598 34.29164490664699 26.53399171074316 19.21134882444387 68-69 20.18120930677461 34.32648026996526 26.520573446241947 18.971736977018185 70-71 19.725283518666703 34.74986893297646 26.531920568784894 18.99292697957194 72-73 19.585400996893284 34.557930970925206 26.412558272748193 19.44410975943332 74-75 19.966675024277233 33.67627129650086 26.484305198354058 19.87274848086785 76-77 20.360187617184863 32.56998349455861 26.548394531737884 20.521434356518636 78-79 20.00745349064147 32.14266334264039 27.138762768379465 20.71112039833867 80-81 19.5908423287995 31.931754325135405 27.41185332201485 21.065550024050246 82-83 19.27720926707798 31.729353263846562 27.334565470985478 21.65887199808999 84-85 19.340213239696467 31.47219634312222 27.795324471798118 21.392265945383194 86-87 19.50622829395274 31.21670114618414 28.280433163538493 20.996637396324623 88-89 19.849208563855782 31.49355003133517 28.03163426859366 20.625607136215386 90-91 20.235775069105124 31.059880436688083 27.411413276313983 21.292931217892814 92-93 19.911415459764545 31.253385890011025 27.213992663575393 21.621205986649038 94-95 19.615334665389796 31.263329608922103 27.439228756231827 21.68210696945627 96-97 19.75105222881194 31.772564053509143 27.092077379288014 21.3843063383909 98-99 20.003870330677813 31.41292087086855 27.005784157691693 21.57742464076194 100-101 20.01085675067496 31.322406313618085 26.77533718562192 21.891399750085032 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 63.0 1 43.5 2 26.5 3 41.5 4 47.0 5 32.0 6 32.0 7 46.5 8 54.0 9 48.5 10 55.0 11 69.0 12 68.0 13 65.0 14 85.5 15 99.5 16 100.0 17 124.0 18 161.0 19 213.5 20 281.0 21 405.5 22 618.5 23 906.5 24 1347.0 25 2001.5 26 2888.5 27 4127.0 28 5635.0 29 7475.5 30 9848.5 31 13130.5 32 18720.0 33 28394.5 34 42799.5 35 63573.5 36 92983.5 37 130338.0 38 166252.5 39 189032.5 40 199599.0 41 194829.5 42 182016.5 43 173703.5 44 160244.5 45 146865.0 46 142354.0 47 131405.5 48 110720.0 49 91788.0 50 75328.0 51 63093.0 52 54251.5 53 45235.5 54 37182.5 55 29858.0 56 23460.5 57 17960.5 58 13639.5 59 10349.5 60 7911.5 61 6141.0 62 4722.0 63 3518.0 64 2529.5 65 1878.0 66 1383.5 67 1023.5 68 766.0 69 567.0 70 414.5 71 316.0 72 240.0 73 188.5 74 157.0 75 111.0 76 81.0 77 63.5 78 52.5 79 44.5 80 28.0 81 20.0 82 20.0 83 12.0 84 4.5 85 3.5 86 5.5 87 6.5 88 5.0 89 3.5 90 2.5 91 1.0 92 0.5 93 1.0 94 0.5 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1535144433436792 2 0.0 3 0.005003106708540707 4 0.00629067093500339 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.83937746637526E-5 14-15 0.0 16-17 1.83937746637526E-5 18-19 0.0 20-21 0.0 22-23 2.023315213012786E-4 24-25 5.5181323991257805E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.277198598688671E-4 40-41 0.0 42-43 0.0 44-45 2.2072529596503122E-4 46-47 0.0011772015784801664 48-49 0.0025751284529253646 50-51 6.437821132313412E-4 52-53 0.0017474085930564973 54-55 0.0022072529596503126 56-57 0.003881086454051799 58-59 0.005389375976479513 60-61 0.0012507766771351768 62-63 7.725385358776093E-4 64-65 0.0010852327051614037 66-67 0.007890929330749866 68-69 0.006750515301597206 70-71 0.00868186164129123 72-73 0.006125126963029617 74-75 0.008442742570662444 76-77 0.004285749496654356 78-79 0.004469687243291882 80-81 0.0016002583957464764 82-83 0.0 84-85 3.126941692837942E-4 86-87 0.0011220202544889087 88-89 0.004396112144636872 90-91 0.006235489611012132 92-93 0.008442742570662444 94-95 0.007651810260121083 96-97 0.009969425867753911 98-99 0.0072471472175185256 100-101 0.006860877949579721 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718311.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.482116862275895 #Duplication Level Percentage of deduplicated Percentage of total 1 90.69964246043644 46.6940959251483 2 4.26896451273705 4.395506598512749 3 1.4220137906345705 2.1962484044765063 4 0.7571913114535357 1.5592724633340347 5 0.40315213430402347 1.0377562645757838 6 0.3989953738326518 1.232467587789602 7 0.25619773119138517 0.9232721075931387 8 0.18017193006259088 0.7420505887027282 9 0.1604306445568838 0.7433378272230963 >10 1.2034690268869588 12.195274636304656 >50 0.11508358544428827 4.208107274660936 >100 0.12471908495715087 12.68468696197697 >500 0.007719586865095314 2.6495066896980353 >1k 0.002105279958518144 1.5477882549341684 >5k 7.177333953935971E-5 0.19942149519948785 >10k+ 7.177333905870748E-5 6.991206919869968 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 189205 6.960388270510622 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 5397 0.19854240372054557 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00452486856728314 0.0 0.0 2 0.004892744060558192 0.0 0.0 3 0.005665282596435802 0.0 0.0 4 0.006106733188365864 0.0 0.0 5 0.007467872513483556 0.0 0.0 6 0.00890258693725626 0.0 0.0 7 0.011440927840854119 0.0 0.0 8 0.01350103060319441 0.0 0.0 9 0.01526683297091466 0.0 0.0 10-11 0.026027191149209934 0.0 0.0 12-13 0.035720710397007555 0.0 0.0 14-15 0.04672018764593161 0.0 0.0 16-17 0.056910338809650546 0.0 0.0 18-19 0.0693077429330198 0.0 0.0 20-21 0.07708830961578717 0.0 0.0 22-23 0.0844826070306157 0.0 0.0 24-25 0.09292534960127814 0.0 0.0 26-27 0.10092664158001052 0.0 0.0 28-29 0.11152145578633203 0.0 0.0 30-31 0.2137908429167965 0.0 0.0 32-33 0.2274942050412922 0.0 0.0 34-35 0.2379602628249674 0.0 0.0 36-37 0.24927243424317527 0.0 0.0 38-39 0.26384030377686735 0.0 1.83937746637526E-5 40-41 0.27976931263567706 0.0 3.67875493275052E-5 42-43 0.29700427949561325 0.0 3.67875493275052E-5 44-45 0.31876411492283263 0.0 3.67875493275052E-5 46-47 0.3417011519285321 0.0 7.35750986550104E-5 48-49 0.36612808468199554 0.0 7.35750986550104E-5 50-51 0.39471201050946714 0.0 7.35750986550104E-5 52-53 0.42636769670578534 0.0 7.35750986550104E-5 54-55 0.4618860755814916 0.0 7.35750986550104E-5 56-57 0.49928061947290064 0.0 7.35750986550104E-5 58-59 0.5412000319315928 0.0 7.35750986550104E-5 60-61 0.5877362818308869 0.0 7.35750986550104E-5 62-63 0.6362222718445387 0.0 7.35750986550104E-5 64-65 0.6871546338884698 0.0 7.35750986550104E-5 66-67 0.7424463205277101 0.0 1.1036264798251561E-4 68-69 0.8016558811703296 0.0 1.2875642264626822E-4 70-71 0.864305077675071 0.0 1.471501973100208E-4 72-73 0.9300260345486591 0.0 2.2072529596503122E-4 74-75 1.000970823426753 0.0 2.2072529596503122E-4 76-77 1.076937112788051 0.0 2.5751284529253643E-4 78-79 1.1632223097357146 0.0 2.5751284529253643E-4 80-81 1.250335226543247 0.0 2.5751284529253643E-4 82-83 1.3503605731647337 0.0 2.5751284529253643E-4 84-85 1.456492652974586 0.0 2.5751284529253643E-4 86-87 1.568529134451503 0.0 2.5751284529253643E-4 88-89 1.6877759755966113 0.0 2.943003946200416E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 27545 0.0 75.29663 3 CGGAAGA 27480 0.0 75.22855 4 GATCGGA 27705 0.0 75.08483 1 ATCGGAA 27925 0.0 74.32303 2 GAAGAGC 27985 0.0 73.89682 6 GGAAGAG 29985 0.0 69.09463 5 CGGTGCG 35 3.3840188E-6 67.972336 1 AGAGCAC 34445 0.0 60.01024 8 GAGCACA 34535 0.0 59.88135 9 AAGAGCA 34905 0.0 59.09692 7 CGTGCGG 70 7.8212906E-8 47.580635 1 GCCGTCT 23410 0.0 44.48615 50-51 CGTCTTC 23415 0.0 44.44704 52-53 ATGCCGT 23385 0.0 44.329773 48-49 TGCCGTC 23415 0.0 44.212936 50-51 TATGCCG 23385 0.0 44.116505 48-49 TCGTATG 23645 0.0 44.043205 44-45 TCTCGTA 22900 0.0 44.024136 42-43 CCGTCTT 23530 0.0 44.00775 52-53 CGTATGC 23455 0.0 44.005093 46-47 >>END_MODULE