FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34139-1.341000000356ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34139-1.341000000356ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718311
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1892056.960388270510622TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT53970.19854240372054557TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG275450.075.296633
CGGAAGA274800.075.228554
GATCGGA277050.075.084831
ATCGGAA279250.074.323032
GAAGAGC279850.073.896826
GGAAGAG299850.069.094635
CGGTGCG353.3840188E-667.9723361
AGAGCAC344450.060.010248
GAGCACA345350.059.881359
AAGAGCA349050.059.096927
CGTGCGG707.8212906E-847.5806351
GCCGTCT234100.044.4861550-51
CGTCTTC234150.044.4470452-53
ATGCCGT233850.044.32977348-49
TGCCGTC234150.044.21293650-51
TATGCCG233850.044.11650548-49
TCGTATG236450.044.04320544-45
TCTCGTA229000.044.02413642-43
CCGTCTT235300.044.0077552-53
CGTATGC234550.044.00509346-47
Produced by FastQC (version 0.11.2)