##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34098-1.34100000035707.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2294152 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6696674849792 33.0 33.0 33.0 33.0 33.0 2 32.69533099812044 33.0 33.0 33.0 33.0 33.0 3 32.728624781618656 33.0 33.0 33.0 33.0 33.0 4 36.65891754338858 37.0 37.0 37.0 37.0 37.0 5 36.659791068769636 37.0 37.0 37.0 37.0 37.0 6 36.53795999567596 37.0 37.0 37.0 37.0 37.0 7 36.42193542537722 37.0 37.0 37.0 37.0 37.0 8 36.363207843246656 37.0 37.0 37.0 37.0 37.0 9 36.40933469098822 37.0 37.0 37.0 37.0 37.0 10-11 36.32607734796997 37.0 37.0 37.0 37.0 37.0 12-13 36.33172431469231 37.0 37.0 37.0 37.0 37.0 14-15 38.184151921930194 40.0 37.0 40.0 37.0 40.0 16-17 38.18319274398557 40.0 37.0 40.0 37.0 40.0 18-19 38.1506789872685 40.0 37.0 40.0 37.0 40.0 20-21 38.14685927523547 40.0 37.0 40.0 37.0 40.0 22-23 37.949115185044406 40.0 37.0 40.0 33.0 40.0 24-25 38.018715412056395 40.0 37.0 40.0 35.0 40.0 26-27 37.94156751601463 40.0 37.0 40.0 33.0 40.0 28-29 37.76196716695319 40.0 37.0 40.0 33.0 40.0 30-31 37.70018922024347 40.0 37.0 40.0 33.0 40.0 32-33 37.59862903591392 40.0 37.0 40.0 33.0 40.0 34-35 37.491416654171125 40.0 37.0 40.0 33.0 40.0 36-37 37.429284981988985 40.0 37.0 40.0 33.0 40.0 38-39 37.366638304698206 40.0 37.0 40.0 33.0 40.0 40-41 37.31796607199523 40.0 37.0 40.0 33.0 40.0 42-43 36.90908950235207 37.0 37.0 40.0 33.0 40.0 44-45 36.73130376714359 37.0 37.0 40.0 33.0 40.0 46-47 36.92102833639619 37.0 37.0 40.0 33.0 40.0 48-49 36.67363234868483 37.0 37.0 40.0 33.0 40.0 50-51 36.56581538625165 37.0 37.0 40.0 33.0 40.0 52-53 36.450585663024945 37.0 37.0 40.0 33.0 40.0 54-55 36.19232444057761 37.0 37.0 40.0 33.0 40.0 56-57 36.05210160442726 37.0 37.0 40.0 33.0 40.0 58-59 35.85644238045256 37.0 37.0 40.0 30.0 40.0 60-61 35.56339444814468 37.0 33.0 40.0 27.0 40.0 62-63 35.20167081344218 37.0 33.0 37.0 27.0 40.0 64-65 34.893667899947346 37.0 33.0 37.0 27.0 40.0 66-67 34.347986532714486 37.0 33.0 37.0 27.0 40.0 68-69 34.10903876465029 37.0 33.0 37.0 27.0 40.0 70-71 33.625246496308876 37.0 33.0 37.0 27.0 37.0 72-73 32.750321251599715 37.0 33.0 37.0 22.0 37.0 74-75 32.08955465897639 35.0 33.0 37.0 22.0 37.0 76-77 29.46196306957865 33.0 27.0 33.0 18.5 37.0 78-79 31.705679048293227 33.0 33.0 37.0 22.0 37.0 80-81 32.07968892209409 37.0 33.0 37.0 22.0 37.0 82-83 32.01875769347454 37.0 33.0 37.0 15.0 37.0 84-85 31.84530645746228 35.0 33.0 37.0 15.0 37.0 86-87 31.67818370360813 33.0 33.0 37.0 15.0 37.0 88-89 31.54667759590472 33.0 33.0 37.0 15.0 37.0 90-91 31.40869480313423 33.0 33.0 37.0 15.0 37.0 92-93 31.28334107766181 33.0 33.0 37.0 15.0 37.0 94-95 31.120480465113033 33.0 33.0 37.0 15.0 37.0 96-97 30.951552469060463 33.0 33.0 37.0 15.0 37.0 98-99 30.809432199784496 33.0 33.0 37.0 15.0 37.0 100-101 29.28428238407917 33.0 30.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15326449532693687 2306 2 0.1637129463349396 2306 3 0.14400018186344 2306 4 0.1613634289622894 2306 5 0.11801399961805714 2306 6 0.11699966033219056 2306 7 0.2030202165746786 2306 8 0.20160848869191028 2306 9 0.1980348412190409 2306 10-11 0.24652959448032874 2306 12-13 0.2665754274052148 2306 14-15 0.4386531231830233 2306 16-17 0.39695616785191845 2306 18-19 0.39207770759360017 2306 20-21 0.41779041563393804 2306 22-23 0.4091971359789284 2306 24-25 0.3731363350815471 2306 26-27 0.40326556274928294 2306 28-29 0.5504546416334151 2306 30-31 0.43245962241190483 2306 32-33 0.3596688469150209 2306 34-35 0.4502508472873714 2306 36-37 0.41830116140101126 2306 38-39 0.4065924590290919 2306 40-41 0.46315438413069643 2306 42-43 0.30264870027605184 2306 44-45 0.4850394103507085 2306 46-47 0.4450832677117518 2306 48-49 0.32681413461352093 2306 50-51 0.36848669891923436 2306 52-53 0.5494742873608587 2306 54-55 0.37762463687740677 2306 56-57 0.35644499392189033 2306 58-59 0.32990914434283525 2306 60-61 0.5600872353884085 2306 62-63 0.3592312047237911 2306 64-65 0.24505628618083364 2306 66-67 0.31501486023127256 2306 68-69 0.3576098509906416 2306 70-71 0.41479919267980137 2306 72-73 -0.09357682183507876 2306 74-75 0.022086772920943076 2306 76-77 -0.3446017073862535 2306 78-79 -0.12628831476634517 2306 80-81 0.07350373186238102 2306 82-83 0.11732615708849892 2306 84-85 0.14758049450582789 2306 86-87 0.018902019614259302 2306 88-89 0.11448345856797815 2306 90-91 -0.058756651610544 2306 92-93 -0.1124342602654238 2306 94-95 -0.06585873844148438 2306 96-97 0.038017571691771224 2306 98-99 0.04199919074213199 2306 100-101 0.08274303962586416 2307 1 0.09749766387132297 2307 2 0.11059305690300647 2307 3 0.10594269022014657 2307 4 0.17046121728822783 2307 5 0.1252809733487652 2307 6 0.055471156847232805 2307 7 0.1097968884633076 2307 8 0.17505620494222285 2307 9 0.2130822297676147 2307 10-11 0.21688274939132413 2307 12-13 0.2233345885137723 2307 14-15 0.30813808736409243 2307 16-17 0.3195907272525531 2307 18-19 0.24689411019144814 2307 20-21 0.2787895307141568 2307 22-23 0.3490491698873015 2307 24-25 0.3812891666685019 2307 26-27 0.3610095488907561 2307 28-29 0.38104613076891525 2307 30-31 0.34013716139683936 2307 32-33 0.25926281570205134 2307 34-35 0.22240026427220272 2307 36-37 0.3243497288003212 2307 38-39 0.3177698088350738 2307 40-41 0.3146618746795653 2307 42-43 0.1001122950515807 2307 44-45 0.2138354191436207 2307 46-47 0.08926218410716302 2307 48-49 0.25439314419129744 2307 50-51 0.2804359301647281 2307 52-53 0.1283861487255109 2307 54-55 0.1118935461746915 2307 56-57 0.10609399658915208 2307 58-59 0.1045782375033042 2307 60-61 0.0642704356710837 2307 62-63 0.011468675916994187 2307 64-65 -0.0032740922083647206 2307 66-67 0.007787129409493332 2307 68-69 0.1039155454337859 2307 70-71 -0.07310695579284499 2307 72-73 -0.236229642477781 2307 74-75 -0.061248123874896976 2307 76-77 -0.33053820579308635 2307 78-79 -0.05145709054393066 2307 80-81 0.13208119986611422 2307 82-83 0.11374410813961333 2307 84-85 0.04650326883878009 2307 86-87 -9.419155583287875E-4 2307 88-89 -0.0166944853537494 2307 90-91 0.05199590543238841 2307 92-93 0.040753639785545914 2307 94-95 0.08485029180526027 2307 96-97 0.1790456420976163 2307 98-99 -0.04040059912851035 2307 100-101 -0.24723025643452345 2201 1 0.14180965487903308 2201 2 0.1796137388755099 2201 3 0.14376742963188605 2201 4 0.17241930783411874 2201 5 0.07431750379606683 2201 6 0.059325609278126024 2201 7 0.13250930324545607 2201 8 0.12583980004814777 2201 9 0.13248211233960205 2201 10-11 0.16459799938522934 2201 12-13 0.0984140103315525 2201 14-15 0.22927311952759766 2201 16-17 0.20389011412903102 2201 18-19 0.2126427424709405 2201 20-21 0.25201522648948327 2201 22-23 0.2056176018501148 2201 24-25 0.14712622884361082 2201 26-27 0.17512430964912795 2201 28-29 0.20381869510668338 2201 30-31 0.2514617866823343 2201 32-33 0.37364329681236796 2201 34-35 0.33656483862437625 2201 36-37 0.29987833429245114 2201 38-39 0.39124485968944356 2201 40-41 0.24176664257262104 2201 42-43 0.22669265643700243 2201 44-45 0.2744449250702914 2201 46-47 0.27828553620313556 2201 48-49 0.35849609037107655 2201 50-51 0.3667119261853813 2201 52-53 0.2682431966312606 2201 54-55 0.26215172988779756 2201 56-57 0.22137298860319277 2201 58-59 0.3562675212642148 2201 60-61 0.2537663709105402 2201 62-63 0.10217248313982452 2201 64-65 0.15113469795294776 2201 66-67 0.2348278717612402 2201 68-69 0.04297112563295258 2201 70-71 0.3364044259673378 2201 72-73 0.012525663674843202 2201 74-75 0.11901033259275806 2201 76-77 -0.08542618262904611 2201 78-79 0.15155914409388416 2201 80-81 0.3509078672218493 2201 82-83 0.49584265292232743 2201 84-85 0.5236661047395934 2201 86-87 0.3919625902032493 2201 88-89 0.1781289204318064 2201 90-91 0.14959178942269702 2201 92-93 -0.05431969529916003 2201 94-95 0.09245181750807063 2201 96-97 0.2375776196972872 2201 98-99 0.1718208144004052 2201 100-101 0.09321881239580421 1101 1 0.12779407598591064 1101 2 0.1083238455428841 1101 3 0.03958873295709964 1101 4 0.11376816059517125 1101 5 0.1194280129503511 1101 6 0.12007708508952675 1101 7 0.09626931129936622 1101 8 0.015975636770747315 1101 9 0.08091204305197408 1101 10-11 0.1263393133933448 1101 12-13 0.028905862266867643 1101 14-15 0.18700703896032422 1101 16-17 0.16006632681905586 1101 18-19 0.03657180441459218 1101 20-21 0.12475078122086813 1101 22-23 0.18211363931541058 1101 24-25 0.0995909395157284 1101 26-27 0.11390128360066853 1101 28-29 0.0883799108299641 1101 30-31 0.3082891977307014 1101 32-33 0.23765608500441004 1101 34-35 0.12543056730250868 1101 36-37 0.0092255136761068 1101 38-39 0.10999591924299779 1101 40-41 0.12634794091108859 1101 42-43 0.13922165234276207 1101 44-45 0.023658051693530524 1101 46-47 0.0984403705580732 1101 48-49 0.17174107063013366 1101 50-51 0.2598198595486565 1101 52-53 0.21198214141240612 1101 54-55 -0.029265794984645765 1101 56-57 0.04936578077003162 1101 58-59 0.02910607112205099 1101 60-61 0.08268844045272061 1101 62-63 0.04991621436452931 1101 64-65 -0.07524358235966844 1101 66-67 0.04990610789209882 1101 68-69 0.10300223542956388 1101 70-71 -0.13185330544828844 1101 72-73 -0.23593058308781423 1101 74-75 -0.13010003363589817 1101 76-77 -0.1442932831845276 1101 78-79 -0.07775198047518828 1101 80-81 0.004515617755082246 1101 82-83 -0.033091570514244495 1101 84-85 0.12020010617197485 1101 86-87 0.06119005111909104 1101 88-89 -0.12182606321259826 1101 90-91 -0.2010050293867316 1101 92-93 -0.25573789306962524 1101 94-95 -0.2728656886379923 1101 96-97 -0.17661249537870205 1101 98-99 -0.1783497052594356 1101 100-101 -0.15038878686578272 2304 1 0.033613564539216156 2304 2 0.07937043169738445 2304 3 0.08122461344190413 2304 4 0.11526174050601412 2304 5 0.09748304640106653 2304 6 0.06020786334980244 2304 7 0.0867475593315703 2304 8 0.11666985617000591 2304 9 0.13889232549269082 2304 10-11 0.07441688737019803 2304 12-13 0.1682520429799581 2304 14-15 0.13520271665239392 2304 16-17 0.10263371435046764 2304 18-19 0.161371784250548 2304 20-21 0.07639343106495744 2304 22-23 7.54582416782057E-4 2304 24-25 0.10025779341999197 2304 26-27 0.16483855180412377 2304 28-29 0.20191934954926438 2304 30-31 0.0704481192421369 2304 32-33 0.22993685498303051 2304 34-35 0.12662960806989076 2304 36-37 0.04724050708678362 2304 38-39 0.126498032867417 2304 40-41 0.11911120777507733 2304 42-43 0.011042393947668927 2304 44-45 0.23667070463423556 2304 46-47 0.126596944398095 2304 48-49 0.1807760048449154 2304 50-51 0.09824713574571575 2304 52-53 0.0911980479664436 2304 54-55 0.018346565577353147 2304 56-57 0.1321201178409126 2304 58-59 0.08428571119377182 2304 60-61 0.14093769690341418 2304 62-63 0.1607601518271764 2304 64-65 -0.046011472552017096 2304 66-67 -0.04416998258686533 2304 68-69 -0.0756829245505557 2304 70-71 -0.08967155535287219 2304 72-73 -0.025652319212426278 2304 74-75 -0.12235163775800117 2304 76-77 -0.3489439770438558 2304 78-79 0.09257446107427825 2304 80-81 0.08139249463196307 2304 82-83 0.15715731146126188 2304 84-85 0.21740522495982972 2304 86-87 0.136055924186401 2304 88-89 0.19504620473880507 2304 90-91 0.13316799255607847 2304 92-93 0.1425690469639349 2304 94-95 0.07646889774695254 2304 96-97 0.04648326224942778 2304 98-99 0.06534686901267861 2304 100-101 0.2000436599695412 2202 1 0.07615455487368195 2202 2 0.08310065750748663 2202 3 -0.005064616044073489 2202 4 0.07220763614842696 2202 5 0.1094337448617253 2202 6 0.041495728524822084 2202 7 0.06466879311235374 2202 8 0.11399780324804709 2202 9 0.12714838114769833 2202 10-11 0.1310956886400092 2202 12-13 0.12900758285887548 2202 14-15 0.09992573934432869 2202 16-17 0.03376773405422995 2202 18-19 0.008961168802109398 2202 20-21 0.02049805485898304 2202 22-23 -0.05579355557606647 2202 24-25 -0.07986441778421494 2202 26-27 0.02228214364665604 2202 28-29 -0.10171559633307226 2202 30-31 -0.05514014280318236 2202 32-33 -0.03374552011596421 2202 34-35 -0.008858671057041079 2202 36-37 -0.07449673771497345 2202 38-39 -0.07812420828508237 2202 40-41 -0.06331535791039755 2202 42-43 -0.1273770462633408 2202 44-45 0.05303988496065415 2202 46-47 -0.11605951322950858 2202 48-49 -0.2834972389097459 2202 50-51 -0.05988027536887586 2202 52-53 -0.24492458029541808 2202 54-55 -0.35625883798139313 2202 56-57 -0.17320368971803646 2202 58-59 -0.3320717116561056 2202 60-61 -0.24289477458469833 2202 62-63 -0.3548097419340479 2202 64-65 -0.5149698488666985 2202 66-67 -0.2609484562002038 2202 68-69 -0.2833503521256162 2202 70-71 -0.2535098833953384 2202 72-73 -0.5128902439512686 2202 74-75 -0.48876651695547935 2202 76-77 -0.7520546060244833 2202 78-79 -0.4878292640492319 2202 80-81 -0.07998585874454278 2202 82-83 -0.15116709284508545 2202 84-85 -0.13005870953549348 2202 86-87 -0.19834700675284012 2202 88-89 -0.20254207061685392 2202 90-91 -0.2056563041582642 2202 92-93 -0.4386354066794418 2202 94-95 -0.29271231602393755 2202 96-97 -0.20461783652003263 2202 98-99 -0.3201239957386264 2202 100-101 -0.16171625679639945 1102 1 0.09750875710999196 1102 2 0.12245542678856225 1102 3 0.04148727579524092 1102 4 0.028911491845882153 1102 5 0.02757172713432965 1102 6 -0.024555466925576752 1102 7 0.028424285065078436 1102 8 0.010835597402518715 1102 9 0.019664218045761572 1102 10-11 0.06470385242902665 1102 12-13 -0.006735483777163154 1102 14-15 -0.05631547818770599 1102 16-17 -0.0868211646993089 1102 18-19 0.002951942942381436 1102 20-21 -0.15406864222942573 1102 22-23 -0.18111334605922735 1102 24-25 0.0100335969382499 1102 26-27 -0.02209914635949417 1102 28-29 -0.1751910426738732 1102 30-31 -0.10484037479484698 1102 32-33 0.002811172978290699 1102 34-35 -0.18060970115387676 1102 36-37 0.004398539050477268 1102 38-39 -0.0066260718756367964 1102 40-41 0.10533430983034009 1102 42-43 -0.04291283113401079 1102 44-45 -0.08854777829753857 1102 46-47 -0.05250755723549361 1102 48-49 -0.1717258862596438 1102 50-51 -0.09918579039658937 1102 52-53 -0.2121319055204296 1102 54-55 -0.2394182292981597 1102 56-57 -0.2317284006597191 1102 58-59 -0.21584626376316862 1102 60-61 -0.30764518176009403 1102 62-63 -0.29676805379624227 1102 64-65 -0.1735700967935685 1102 66-67 -0.08436440423075453 1102 68-69 0.025417939724462713 1102 70-71 -0.34270778408800595 1102 72-73 -0.5562270540554195 1102 74-75 -0.6854147443197363 1102 76-77 -0.624784512669045 1102 78-79 -0.286093879421113 1102 80-81 -0.1497037022360459 1102 82-83 -0.1360331026504049 1102 84-85 -0.020853027498606735 1102 86-87 -0.07315335268739886 1102 88-89 -0.08439467208993179 1102 90-91 -0.2575535241902607 1102 92-93 -0.32467058414996686 1102 94-95 -0.3637528845587461 1102 96-97 -0.17772770454760334 1102 98-99 -0.3295495628351297 1102 100-101 -0.4331399944492027 2305 1 0.034918628988293676 2305 2 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90-91 -0.0248783110470292 1213 92-93 0.055498671284947676 1213 94-95 0.14366364850215163 1213 96-97 0.10405240361047063 1213 98-99 0.061576581756717985 1213 100-101 0.2481762316224838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 7.0 8 20.0 9 69.0 10 43.0 11 35.0 12 22.0 13 65.0 14 147.0 15 420.0 16 881.0 17 1490.0 18 2106.0 19 3062.0 20 4021.0 21 5412.0 22 7565.0 23 10215.0 24 13741.0 25 17924.0 26 24235.0 27 35499.0 28 58360.0 29 88918.0 30 73817.0 31 54950.0 32 69151.0 33 98861.0 34 151471.0 35 264873.0 36 500382.0 37 674875.0 38 131445.0 39 70.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.83279164580315 24.311634836976427 20.341767360885257 14.513806156335168 2 29.852817075764815 27.66760877221736 28.824637600298498 13.654936551719329 3 34.78527155403335 18.115836841341267 30.60744007180355 16.491451532821834 4 29.79241318581679 17.52752857479141 28.841140724853748 23.838917514538053 5 36.68793523707235 16.149583811360362 30.119887435531734 17.042593516035552 6 33.06132287660103 17.07929553054898 32.926763353082094 16.932618239767898 7 31.67008986326974 24.463069578650412 25.458339290509084 18.408501267570763 8 28.99585554923998 30.914342205747484 22.015629304422724 18.074172940589815 9 33.49102413440783 23.300199812392552 24.082144513528313 19.126631539671305 10-11 26.010504099118105 29.36202570710223 27.942677730159115 16.684792463620546 12-13 22.285513651137382 28.478484074381377 27.570260787276506 21.665741487204734 14-15 22.195172769720575 28.67870132406222 27.910661542914333 21.215464363302868 16-17 25.313144028817618 25.36826243422406 28.436956226091382 20.881637310866935 18-19 20.749300830982428 25.41126307236835 32.127208659234434 21.712227437414782 20-21 24.07850482444058 25.76854105569291 31.82579009586113 18.327164024005384 22-23 20.371392697552054 32.71477413079406 28.258480216080173 18.65535295557371 24-25 20.680059560133763 24.653859029392997 32.1652837301103 22.50079768036294 26-27 20.449037378517204 24.794324874724953 28.816573618487357 25.94006412827049 28-29 23.504283935850808 29.22201754722442 28.138654282715358 19.135044234209417 30-31 19.255132179559155 26.830785405674952 31.252637139997695 22.661445274768194 32-33 18.999307805236967 30.54322468607137 28.200420024479634 22.25704748421203 34-35 19.550796982937484 30.45707084796474 31.963422650286468 18.028709518811308 36-37 19.149624785105782 31.1654589582556 32.292694642726374 17.39222161391224 38-39 19.52497958923916 27.073757275624388 31.599737242784094 21.801525892352362 40-41 23.26626570514944 26.38329979879276 27.92249598108582 22.42793851497198 42-43 19.88767527173439 30.153494624593314 31.40729123440818 18.55153886926411 44-45 19.403670464585808 26.79614375862792 31.65467312308562 22.14551265370065 46-47 23.067331697998476 26.568575634963622 28.280838793833386 22.08325387320452 48-49 19.607143129581743 30.8834539147833 31.116726984635534 18.392675970999424 50-51 23.043983042923823 27.17626235999417 30.486950369974352 19.292804227107652 52-53 19.242256817988636 26.72224365387088 27.418699585679008 26.616799942461473 54-55 23.36526612524623 26.67049970598351 31.06396482438127 18.90026934438899 56-57 19.912207667661995 26.746888690307536 30.387938362285045 22.95296527974543 58-59 19.726265360656665 26.813396628611034 30.799059289192286 22.66127872154001 60-61 22.840147778473522 27.057339677988345 30.78743625794671 19.315076285591424 62-63 19.3457397631262 27.158473375344848 30.53807255289907 22.957714308629885 64-65 23.382240217006814 27.084201948327497 30.385596403677834 19.147961430987852 66-67 20.27943540107945 33.839709461465326 26.76182143702535 19.11903370042987 68-69 20.572446398947847 33.70666857312121 26.824818114863742 18.896066913067198 70-71 19.980452649332996 34.5616035802516 26.714654453495918 18.743289316919487 72-73 19.84967240484919 34.495246273959346 26.576314630839715 19.078766690351745 74-75 20.648808930887714 33.26464470417862 26.439491413521747 19.647054951411913 76-77 20.703376671836597 32.21783112194501 26.770331215666378 20.308460990552017 78-79 20.52556705178841 31.790950143992912 27.34640447089661 20.337078333322072 80-81 19.7631107488307 31.834589448631494 27.611709987751244 20.79058981478656 82-83 19.07927199243991 31.64066722693178 27.852077804783644 21.42798297584467 84-85 19.22781018404298 31.40406113320123 28.094277676408307 21.27385100634748 86-87 19.167155780585492 31.489186786448542 28.769509978686763 20.574147454279203 88-89 19.468738189447475 31.67385852576406 28.636451413648217 20.22095187114025 90-91 20.15329236382579 31.12523744187238 27.763332980962147 20.958137213339683 92-93 19.527121062021358 31.71072320289111 27.566128397693472 21.196027337394057 94-95 19.475689145923102 31.690871335378862 27.655616777127783 21.177822741570253 96-97 19.85212026203512 32.347395756816695 27.080340783411245 20.720143197736945 98-99 20.09070773217156 31.620698869771545 27.117570874134305 21.171022523922595 100-101 20.109238902283952 31.71589951538015 26.713573845785653 21.46128773655024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 58.0 1 37.5 2 24.5 3 57.0 4 63.5 5 41.5 6 39.0 7 45.5 8 46.0 9 44.0 10 47.0 11 47.0 12 56.5 13 62.5 14 59.0 15 63.5 16 76.0 17 90.0 18 104.5 19 124.5 20 143.5 21 169.5 22 219.5 23 322.5 24 472.0 25 664.5 26 1052.5 27 1696.5 28 2402.0 29 3181.5 30 4165.5 31 5571.5 32 9573.0 33 18546.0 34 33721.0 35 55079.0 36 85675.0 37 124773.5 38 157179.0 39 173937.0 40 180657.0 41 171802.0 42 160191.5 43 158625.0 44 150074.0 45 135311.5 46 125244.0 47 109591.0 48 86657.0 49 70590.5 50 59387.0 51 47959.0 52 37529.5 53 29948.0 54 23068.0 55 16178.0 56 11671.0 57 9011.0 58 7110.5 59 5639.5 60 4292.5 61 3208.5 62 2445.5 63 1888.0 64 1491.5 65 1170.5 66 901.0 67 688.5 68 562.0 69 473.0 70 340.0 71 223.0 72 153.0 73 108.0 74 75.5 75 54.0 76 42.0 77 26.0 78 18.0 79 14.5 80 10.5 81 8.0 82 4.0 83 1.5 84 1.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1512977344134129 2 0.0 3 0.005012745450170695 4 0.007497323629820518 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.0897272717762379E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.5256181804867332E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.281927265499409E-4 40-41 0.0 42-43 0.0 44-45 4.358909087104952E-5 46-47 0.0010679327263407132 48-49 0.0025935509068274465 50-51 2.833290906618219E-4 52-53 0.0016127963622288323 54-55 0.00274611272487612 56-57 0.004446087268847051 58-59 0.006189650903689032 60-61 0.0018525363620196045 62-63 8.281927265499409E-4 64-65 0.0013294672715670104 66-67 0.008935763628565152 68-69 0.008303721810934934 70-71 0.010243436354696638 72-73 0.007061432721110023 74-75 0.009829339991421666 76-77 0.0051435127227838435 78-79 0.00503453999560622 80-81 0.0018307418165840797 82-83 0.0 84-85 6.102472721946933E-4 86-87 0.0016345909076643569 88-89 0.005361458177139091 90-91 0.007388350902642895 92-93 0.009785750900550616 94-95 0.009044736355742775 96-97 0.011376752717343924 98-99 0.008325516356370459 100-101 0.00823833817462836 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2294152.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.42110462060778 #Duplication Level Percentage of deduplicated Percentage of total 1 83.8849021867485 28.874109942595204 2 7.1489857994812525 4.921519762703671 3 2.502947242363647 2.5846262666778244 4 1.235112813658238 1.7005578950873381 5 0.7312025220817009 1.2584399255713248 6 0.6587768856421818 1.3605496861396627 7 0.40068868787645806 0.9654503071982732 8 0.31877899983581987 0.8778180243361171 9 0.35175794683618034 1.0897107380260518 >10 2.13924518327737 14.656932584646611 >50 0.3116048169260517 7.6742791360610685 >100 0.29160822620770405 20.553660167635925 >500 0.019287709112034396 4.278453244084245 >1k 0.004973923956179908 2.760550673278378 >5k 0.0 0.0 >10k+ 1.2705599670767806E-4 6.443341645958016 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 147330 6.421980758031726 TruSeq Adapter, Index 27 (97% over 39bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 4368 0.1903971489247443 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2974 0.12963395625050128 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 2871 0.12514427989078317 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2736 0.1192597526231915 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2520 0.10984450899504479 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2356 0.10269589809219268 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2313 0.10082156718473755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004707621814073348 0.0 0.0 2 0.004969156359299645 0.0 0.0 3 0.0059717054493337845 0.0 0.0 4 0.006451185448915329 0.0 0.0 5 0.007715269084175765 0.0 0.0 6 0.0091537090829204 0.0 0.0 7 0.011028039990375528 0.0 0.0 8 0.01268442544347541 0.0 0.0 9 0.01460234544180159 0.0 0.0 10-11 0.021271476345072166 0.0 0.0 12-13 0.028921361792941358 0.0 0.0 14-15 0.03620073996840663 0.0 0.0 16-17 0.04223782905404698 0.0 0.0 18-19 0.049974892683658276 0.0 0.0 20-21 0.05745042176804327 0.0 0.0 22-23 0.06394519630782965 0.0 0.0 24-25 0.07113739630155282 0.0 4.358909087104952E-5 26-27 0.07955009083966538 0.0 4.358909087104952E-5 28-29 0.08863841628627919 0.0 4.358909087104952E-5 30-31 0.14748368896219605 0.0 4.358909087104952E-5 32-33 0.15864249622518473 0.0 4.358909087104952E-5 34-35 0.16821030167138012 0.0 4.358909087104952E-5 36-37 0.1798049998430793 0.0 4.358909087104952E-5 38-39 0.19370991983094407 0.0 4.358909087104952E-5 40-41 0.20996865072584553 0.0 4.358909087104952E-5 42-43 0.22803632889189557 0.0 4.358909087104952E-5 44-45 0.24721552887515735 0.0 4.358909087104952E-5 46-47 0.26809470340239006 0.0 4.358909087104952E-5 48-49 0.29174178519993443 0.0 4.358909087104952E-5 50-51 0.3167619233599168 0.0 4.358909087104952E-5 52-53 0.345923025152649 0.0 4.358909087104952E-5 54-55 0.3779392123974349 0.0 4.358909087104952E-5 56-57 0.4130284305486297 0.0 4.358909087104952E-5 58-59 0.44953429415313373 0.0 4.358909087104952E-5 60-61 0.49048624502648475 0.0 4.358909087104952E-5 62-63 0.5327240740805317 0.0 8.717818174209904E-5 64-65 0.5772721249507443 0.0 8.717818174209904E-5 66-67 0.624108603091687 0.0 8.717818174209904E-5 68-69 0.6778539521356911 0.0 8.717818174209904E-5 70-71 0.7325364666334226 0.0 8.717818174209904E-5 72-73 0.7918394247634857 0.0 8.717818174209904E-5 74-75 0.8534743992551497 0.0 8.717818174209904E-5 76-77 0.917702924653641 0.0 8.717818174209904E-5 78-79 0.9882736627738702 0.0 8.717818174209904E-5 80-81 1.0666250536145818 0.0 8.717818174209904E-5 82-83 1.1507302044502719 0.0 8.717818174209904E-5 84-85 1.2449916134589163 0.0 8.717818174209904E-5 86-87 1.3460747151888803 0.0 8.717818174209904E-5 88-89 1.447986009645394 0.0 8.717818174209904E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGAGC 21120 0.0 75.5864 6 TCGGAAG 21180 0.0 75.58565 3 GATCGGA 21200 0.0 75.49178 1 CGGAAGA 21205 0.0 75.267586 4 ATCGGAA 21405 0.0 74.70235 2 GGAAGAG 21750 0.0 73.48436 5 CGACGCG 20 0.0020613857 71.36263 1 GAGCACA 28270 0.0 56.45242 9 AGAGCAC 28330 0.0 56.249035 8 AAGAGCA 28410 0.0 56.090637 7 CGGGGCG 85 1.2732926E-10 50.37362 1 GTTACGG 70 7.827839E-8 47.575085 1 CGAGGCG 105 2.0008883E-11 45.309605 1 ATGCCGT 17690 0.0 45.216564 48-49 GCCGTCT 17715 0.0 45.20687 50-51 CGTCTTC 17695 0.0 45.20428 52-53 CGAGGTG 190 0.0 45.071133 1 TCGTATG 17855 0.0 44.917934 44-45 CTGCTTG 17910 0.0 44.834015 58-59 GTATGCC 17885 0.0 44.736362 46-47 >>END_MODULE