FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34098-1.34100000035707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34098-1.34100000035707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294152
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1473306.421980758031726TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT43680.1903971489247443TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC29740.12963395625050128No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA28710.12514427989078317No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG27360.1192597526231915No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25200.10984450899504479No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC23560.10269589809219268No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG23130.10082156718473755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGAGC211200.075.58646
TCGGAAG211800.075.585653
GATCGGA212000.075.491781
CGGAAGA212050.075.2675864
ATCGGAA214050.074.702352
GGAAGAG217500.073.484365
CGACGCG200.002061385771.362631
GAGCACA282700.056.452429
AGAGCAC283300.056.2490358
AAGAGCA284100.056.0906377
CGGGGCG851.2732926E-1050.373621
GTTACGG707.827839E-847.5750851
CGAGGCG1052.0008883E-1145.3096051
ATGCCGT176900.045.21656448-49
GCCGTCT177150.045.2068750-51
CGTCTTC176950.045.2042852-53
CGAGGTG1900.045.0711331
TCGTATG178550.044.91793444-45
CTGCTTG179100.044.83401558-59
GTATGCC178850.044.73636246-47
Produced by FastQC (version 0.11.2)