##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34042-1.3410000003584a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5256177 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67917081178963 33.0 33.0 33.0 33.0 33.0 2 32.7040862588912 33.0 33.0 33.0 33.0 33.0 3 32.74342511677213 33.0 33.0 33.0 33.0 33.0 4 36.67076546318741 37.0 37.0 37.0 37.0 37.0 5 36.67321591339105 37.0 37.0 37.0 37.0 37.0 6 36.557156465621304 37.0 37.0 37.0 37.0 37.0 7 36.438511678735324 37.0 37.0 37.0 37.0 37.0 8 36.39253035048097 37.0 37.0 37.0 37.0 37.0 9 36.42020293456632 37.0 37.0 37.0 37.0 37.0 10-11 36.35202229681383 37.0 37.0 37.0 37.0 37.0 12-13 36.36615795092136 37.0 37.0 37.0 37.0 37.0 14-15 38.24100168240149 40.0 37.0 40.0 37.0 40.0 16-17 38.241167677572506 40.0 37.0 40.0 37.0 40.0 18-19 38.21686722117615 40.0 37.0 40.0 37.0 40.0 20-21 38.20798414893562 40.0 37.0 40.0 37.0 40.0 22-23 37.98953583564632 40.0 37.0 40.0 33.0 40.0 24-25 38.08384639634472 40.0 37.0 40.0 37.0 40.0 26-27 38.03607327150513 40.0 37.0 40.0 37.0 40.0 28-29 37.84992210117734 40.0 37.0 40.0 33.0 40.0 30-31 37.808523381157066 40.0 37.0 40.0 33.0 40.0 32-33 37.6391971769596 40.0 37.0 40.0 33.0 40.0 34-35 37.57968148713409 40.0 37.0 40.0 33.0 40.0 36-37 37.57541774183023 40.0 37.0 40.0 33.0 40.0 38-39 37.39292636073709 40.0 37.0 40.0 33.0 40.0 40-41 37.16695356720293 40.0 37.0 40.0 33.0 40.0 42-43 37.00863488805648 37.0 37.0 40.0 33.0 40.0 44-45 36.88451197514848 37.0 37.0 40.0 33.0 40.0 46-47 37.00336137843151 37.0 37.0 40.0 33.0 40.0 48-49 36.73243148014232 37.0 37.0 40.0 33.0 40.0 50-51 36.64333602159897 37.0 37.0 40.0 33.0 40.0 52-53 36.51779601409922 37.0 37.0 40.0 33.0 40.0 54-55 36.24025950800363 37.0 37.0 40.0 33.0 40.0 56-57 36.11208193711894 37.0 37.0 40.0 33.0 40.0 58-59 35.940163734973154 37.0 37.0 40.0 33.0 40.0 60-61 35.633347963738665 37.0 35.0 40.0 27.0 40.0 62-63 35.3791762530067 37.0 33.0 40.0 27.0 40.0 64-65 35.01361674083654 37.0 33.0 37.0 27.0 40.0 66-67 34.33390133170934 37.0 33.0 37.0 27.0 40.0 68-69 34.12899679748227 37.0 33.0 37.0 27.0 40.0 70-71 33.5890419976344 37.0 33.0 37.0 27.0 37.0 72-73 32.55139400746969 37.0 33.0 37.0 22.0 37.0 74-75 31.792220372335255 35.0 33.0 37.0 18.5 37.0 76-77 29.180657443613484 33.0 27.0 33.0 15.0 37.0 78-79 31.337196502324787 33.0 33.0 37.0 15.0 37.0 80-81 31.680007541602954 35.0 33.0 37.0 15.0 37.0 82-83 31.614631508794318 37.0 33.0 37.0 15.0 37.0 84-85 31.427303247208 33.0 33.0 37.0 15.0 37.0 86-87 31.26192154487948 33.0 33.0 37.0 15.0 37.0 88-89 31.137038573853204 33.0 33.0 37.0 15.0 37.0 90-91 30.988506665586034 33.0 33.0 37.0 15.0 37.0 92-93 30.858379674048265 33.0 33.0 37.0 15.0 37.0 94-95 30.689527293316036 33.0 33.0 37.0 15.0 37.0 96-97 30.530160704253298 33.0 30.0 37.0 15.0 37.0 98-99 30.388971299862998 33.0 27.0 37.0 15.0 37.0 100-101 28.89766088546866 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13648439634019383 2306 2 0.14777613622703 2306 3 0.12688247540818764 2306 4 0.156326669914435 2306 5 0.18366254208556398 2306 6 0.1482627268877934 2306 7 0.16885523063726993 2306 8 0.20691567781558007 2306 9 0.20940376600307076 2306 10-11 0.21262519268486102 2306 12-13 0.2674025793679391 2306 14-15 0.46164768064318196 2306 16-17 0.36442433518994477 2306 18-19 0.4094117757483673 2306 20-21 0.39561388141886766 2306 22-23 0.46271089453838243 2306 24-25 0.4410564891577522 2306 26-27 0.4984502278873535 2306 28-29 0.4764358188868911 2306 30-31 0.5280225078118193 2306 32-33 0.5830070464594854 2306 34-35 0.5361279757901727 2306 36-37 0.5281629194476878 2306 38-39 0.44364945282629975 2306 40-41 0.620690419464303 2306 42-43 0.5067610000671223 2306 44-45 0.6214851301300044 2306 46-47 0.48145618172069504 2306 48-49 0.49424727269482815 2306 50-51 0.5507721473656986 2306 52-53 0.5383967230199573 2306 54-55 0.5367128369201026 2306 56-57 0.4951112658337351 2306 58-59 0.5861211843555765 2306 60-61 0.5896657347638126 2306 62-63 0.5558261371041766 2306 64-65 0.5478639873676769 2306 66-67 0.4744357043914107 2306 68-69 0.509068135084668 2306 70-71 0.46436370240799363 2306 72-73 0.14393163877563353 2306 74-75 0.16669476209578704 2306 76-77 -0.19855988644327383 2306 78-79 0.11237477593497047 2306 80-81 0.20327969669759582 2306 82-83 0.33594046946565825 2306 84-85 0.3703714853816997 2306 86-87 0.4051711117333632 2306 88-89 0.3192821241673265 2306 90-91 0.319982473971816 2306 92-93 0.2428455859164096 2306 94-95 0.3023509212405564 2306 96-97 0.36481913483864403 2306 98-99 0.2702024665740872 2306 100-101 0.05622458208699044 2307 1 0.11484462538756901 2307 2 0.0955962819844487 2307 3 0.12598645770907524 2307 4 0.15842583701490298 2307 5 0.1323253479158666 2307 6 0.12678498092214596 2307 7 0.13500176889859716 2307 8 0.18616997245378997 2307 9 0.23441482794997626 2307 10-11 0.17283179081083944 2307 12-13 0.16001482559386204 2307 14-15 0.3571252964682827 2307 16-17 0.3807943898489796 2307 18-19 0.30605089599824 2307 20-21 0.38474616928976957 2307 22-23 0.3863021230651924 2307 24-25 0.4024838056595712 2307 26-27 0.4178112377780323 2307 28-29 0.44809576162504783 2307 30-31 0.47447650312675904 2307 32-33 0.48684586738608715 2307 34-35 0.5229548237860016 2307 36-37 0.502104226058826 2307 38-39 0.37921458817248066 2307 40-41 0.37159326954238026 2307 42-43 0.24616170282610028 2307 44-45 0.3920854034251633 2307 46-47 0.3872458095187241 2307 48-49 0.3476813565053476 2307 50-51 0.3922280947889121 2307 52-53 0.379868612660772 2307 54-55 0.3313897630002671 2307 56-57 0.36954970935273224 2307 58-59 0.3102316736840436 2307 60-61 0.26008836620576403 2307 62-63 0.3406358065471764 2307 64-65 0.22023254541036152 2307 66-67 0.21998066795205773 2307 68-69 0.3017747332106424 2307 70-71 0.2960909928817017 2307 72-73 -0.03986423576886722 2307 74-75 0.04714960332431062 2307 76-77 -0.348008090503658 2307 78-79 0.04471242559660382 2307 80-81 0.26779654989904955 2307 82-83 0.24386505301589523 2307 84-85 0.31188892421564063 2307 86-87 0.05992091652253606 2307 88-89 0.13116916216836927 2307 90-91 0.18579780452881778 2307 92-93 0.0821089903932446 2307 94-95 0.03716189208907039 2307 96-97 0.17252117804530798 2307 98-99 0.06439917401812778 2307 100-101 0.03702071691260045 2201 1 0.12785743414099215 2201 2 0.1422603691749984 2201 3 0.10620811172411493 2201 4 0.11565824878056219 2201 5 0.10761063352366307 2201 6 0.09392111514469548 2201 7 0.1419676606000735 2201 8 0.12168631081294023 2201 9 0.19647802472781706 2201 10-11 0.18788018211174773 2201 12-13 0.16867690909457878 2201 14-15 0.3558845645859492 2201 16-17 0.2558500841828035 2201 18-19 0.2495486859309679 2201 20-21 0.2243177880668057 2201 22-23 0.3566990788963622 2201 24-25 0.28697364846439655 2201 26-27 0.2692911759170471 2201 28-29 0.1943997622705922 2201 30-31 0.2621282311822526 2201 32-33 0.2588303983665057 2201 34-35 0.28147997541972103 2201 36-37 0.2088669187067964 2201 38-39 0.2753500254720507 2201 40-41 0.19165373000499386 2201 42-43 0.3876146435449783 2201 44-45 0.2852315607410816 2201 46-47 0.28942228603174414 2201 48-49 0.28204690842422053 2201 50-51 0.32981470219922926 2201 52-53 0.27969181308431956 2201 54-55 0.2914472743844101 2201 56-57 0.27448278326778563 2201 58-59 0.24420722013435636 2201 60-61 0.26145648753828254 2201 62-63 0.25497345832602747 2201 64-65 0.21405689643894021 2201 66-67 0.2503147773535801 2201 68-69 0.2801365593055749 2201 70-71 0.4065925060260014 2201 72-73 0.08795513114293385 2201 74-75 0.001918765368046138 2201 76-77 -0.12123600202201601 2201 78-79 0.08934426827139674 2201 80-81 0.2045630174255173 2201 82-83 0.31525136517004526 2201 84-85 0.36653830897284934 2201 86-87 0.24058473638028488 2201 88-89 0.3049626233415452 2201 90-91 0.2620015132852558 2201 92-93 0.08839639086184548 2201 94-95 0.10361872959716933 2201 96-97 0.16225357384647054 2201 98-99 0.0799443515201439 2201 100-101 0.08615824933504967 1101 1 0.09414094282976038 1101 2 0.10774548376753046 1101 3 0.061200093274194955 1101 4 0.112171802011936 1101 5 0.08859977752565129 1101 6 0.07383709559309182 1101 7 0.08131399578104492 1101 8 -0.028001122651510002 1101 9 0.09853527965071862 1101 10-11 0.1146154130906396 1101 12-13 0.09853380108739174 1101 14-15 0.14631780179337994 1101 16-17 0.15101023505857825 1101 18-19 0.09612904330054306 1101 20-21 0.10018547680640211 1101 22-23 0.1484885182429494 1101 24-25 0.08399985508972918 1101 26-27 0.06924901638537762 1101 28-29 0.10609426290965729 1101 30-31 0.1490774632927696 1101 32-33 0.12270599551801098 1101 34-35 0.04117979304312058 1101 36-37 0.14315489142257576 1101 38-39 0.11396838436913725 1101 40-41 0.10968472686467834 1101 42-43 0.13415043226680012 1101 44-45 -0.04382358392633989 1101 46-47 0.033032593923287834 1101 48-49 -0.04001414899543221 1101 50-51 0.03435702548568287 1101 52-53 0.12585913726604758 1101 54-55 0.024012501202690828 1101 56-57 -0.05448696800074515 1101 58-59 -0.09786509501969931 1101 60-61 0.09290577271447376 1101 62-63 0.016320590468652085 1101 64-65 -0.039667759822513915 1101 66-67 -0.0014499327418491248 1101 68-69 0.002708479559920818 1101 70-71 -0.10324577066162988 1101 72-73 -0.19268345391156316 1101 74-75 0.012184106717008092 1101 76-77 -0.139185707491297 1101 78-79 -0.04158194571150631 1101 80-81 0.2766927020982699 1101 82-83 0.283385368020614 1101 84-85 0.3529696462341292 1101 86-87 0.22419789614439978 1101 88-89 0.21770899615681927 1101 90-91 0.029599683138361144 1101 92-93 0.03980953278547972 1101 94-95 0.13389923535528325 1101 96-97 0.19989503616691806 1101 98-99 0.18971312195286671 1101 100-101 0.16124027322696577 2304 1 0.07359877003119664 2304 2 0.05566620100087505 2304 3 0.08526729031033398 2304 4 0.11432998489286206 2304 5 0.09586519961639084 2304 6 0.07635224517565575 2304 7 0.02831658131760406 2304 8 0.06388746582339877 2304 9 0.06414085631196542 2304 10-11 -0.001569642051670428 2304 12-13 0.02641002766674916 2304 14-15 0.08391594423251547 2304 16-17 0.055754071517391424 2304 18-19 0.02297336638320502 2304 20-21 0.04555687488600313 2304 22-23 0.03401673944178185 2304 24-25 7.120226750885195E-4 2304 26-27 0.07453380279337551 2304 28-29 0.09574217005268082 2304 30-31 0.08961983502376825 2304 32-33 0.1227846030845896 2304 34-35 0.1436805785336972 2304 36-37 0.11907335954921905 2304 38-39 0.15101501588718236 2304 40-41 0.1665938551134971 2304 42-43 -0.024262274132915707 2304 44-45 0.14757969717162212 2304 46-47 0.07575552843415068 2304 48-49 0.17107744593365481 2304 50-51 0.08316226068524912 2304 52-53 0.10438192351373488 2304 54-55 0.10758790800488782 2304 56-57 0.08683030407388515 2304 58-59 0.1135775278927511 2304 60-61 -0.06990009321793877 2304 62-63 0.021568574376182426 2304 64-65 -0.06821795778954254 2304 66-67 0.05764239121610615 2304 68-69 -0.01750703447856239 2304 70-71 0.02298268100626899 2304 72-73 0.03461294312949548 2304 74-75 0.010115882253348474 2304 76-77 -0.3706016149479261 2304 78-79 0.08523560925674545 2304 80-81 0.3548625295477841 2304 82-83 0.39601321867852235 2304 84-85 0.39822923258338605 2304 86-87 0.21567595243408633 2304 88-89 0.26977253985730343 2304 90-91 0.2614635688856133 2304 92-93 0.21837438739219905 2304 94-95 0.24607413441169967 2304 96-97 0.24473001878665812 2304 98-99 0.15206194061600442 2304 100-101 0.33885683446919757 2202 1 3.105494597832603E-4 2202 2 0.02424672446232279 2202 3 0.03326962973468284 2202 4 0.011713929189241412 2202 5 0.007773105133267677 2202 6 0.03469920692137407 2202 7 0.11028803747990423 2202 8 0.03260676245638905 2202 9 -0.004788919099510736 2202 10-11 0.06970762958469123 2202 12-13 0.11659631395365722 2202 14-15 0.023248404786542665 2202 16-17 0.027969226830812488 2202 18-19 0.022484399980932324 2202 20-21 0.06117719148865319 2202 22-23 0.07031899130073072 2202 24-25 -0.024649812988442932 2202 26-27 0.056588838371688155 2202 28-29 0.11237825473723007 2202 30-31 0.06867463039145605 2202 32-33 0.03154918320713307 2202 34-35 -0.007002107643742761 2202 36-37 -0.039038371416559414 2202 38-39 0.07932715655721978 2202 40-41 -0.07814727796971255 2202 42-43 -0.059126075548142865 2202 44-45 -0.0037029897209706064 2202 46-47 -0.07987561237071361 2202 48-49 -0.09899667965863301 2202 50-51 0.08622526170687905 2202 52-53 -0.0710310463036592 2202 54-55 -0.09310665198161416 2202 56-57 -0.026268410925460728 2202 58-59 -0.030069978996479563 2202 60-61 -0.08393805911769903 2202 62-63 -0.27617220995988845 2202 64-65 -0.22992553879068822 2202 66-67 -0.11186972143636353 2202 68-69 0.03679731596307789 2202 70-71 -0.0018060777252912885 2202 72-73 -0.22769296110892157 2202 74-75 -0.1800488229385877 2202 76-77 -0.514577229151989 2202 78-79 -0.09365725953755799 2202 80-81 0.1399862874516451 2202 82-83 0.14788699830020846 2202 84-85 0.09622733887385593 2202 86-87 0.09473360845372625 2202 88-89 0.11396295011055102 2202 90-91 0.11726628044712584 2202 92-93 0.06328295801065309 2202 94-95 0.0805157688031315 2202 96-97 -0.07797826244319239 2202 98-99 -0.017652521094486673 2202 100-101 0.004016076026225335 1102 1 0.036178961744774085 1102 2 0.030833926469853168 1102 3 0.04595817595189544 1102 4 -0.004794323024775338 1102 5 0.033032114255711065 1102 6 0.005570031239898299 1102 7 -0.014163565337831585 1102 8 -0.0708454115637096 1102 9 -0.008940023206463366 1102 10-11 -0.00757076273620072 1102 12-13 0.04464099794741827 1102 14-15 -0.10403551860891014 1102 16-17 -0.09124151960867977 1102 18-19 -0.04718526708381887 1102 20-21 -0.1469922149720091 1102 22-23 -0.15367992151200127 1102 24-25 -0.12618131437018576 1102 26-27 -0.14628929778489663 1102 28-29 -0.1345224314144886 1102 30-31 -0.09623986087467529 1102 32-33 -0.13654255129681303 1102 34-35 -0.09067793854545414 1102 36-37 -0.13864840734149908 1102 38-39 -0.036424476368964065 1102 40-41 -0.13678860727814168 1102 42-43 -0.1295758759630914 1102 44-45 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-0.5015547465136514 1213 82-83 -0.4280495416331185 1213 84-85 -0.4035069213268976 1213 86-87 -0.4489912387414172 1213 88-89 -0.6280014496395516 1213 90-91 -0.47023672691608454 1213 92-93 -0.35798065653025546 1213 94-95 -0.29246194101126477 1213 96-97 -0.2716101412743157 1213 98-99 -0.31997727198800163 1213 100-101 -0.10246483707690146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 25.0 10 37.0 11 30.0 12 56.0 13 137.0 14 326.0 15 841.0 16 1669.0 17 2961.0 18 4481.0 19 6441.0 20 9170.0 21 12700.0 22 17063.0 23 22977.0 24 31098.0 25 42015.0 26 57188.0 27 82335.0 28 133696.0 29 224103.0 30 216285.0 31 134343.0 32 150223.0 33 214580.0 34 337309.0 35 588396.0 36 1137943.0 37 1550498.0 38 277206.0 39 41.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.341626972399695 24.620334554123772 20.49679902153846 14.541239451938068 2 27.955337120496516 29.734120445335076 28.58081072992785 13.729731704240553 3 32.90183836756811 18.080379184358645 32.083655113773766 16.934127334299482 4 27.217119971079846 17.747215008038662 28.968216368427562 26.067448652453933 5 37.08031521769529 16.781664696603634 29.124913411401483 17.013106674299593 6 33.01087463378802 17.663674568036807 32.454120171371706 16.871330626803474 7 29.58751198827589 27.025345607653623 25.336722869111906 18.050419534958586 8 26.714815730139986 32.800189186931874 22.61063506803519 17.874360014892954 9 34.725599994064126 22.90461299153358 23.743987312451615 18.625799701950676 10-11 26.271442152728113 30.020630964292106 26.81302399063045 16.894902892349325 12-13 21.64610736017972 28.845685532600125 26.401532216577223 23.106674890642932 14-15 21.24601207303331 29.208301014216225 26.86073927875717 22.6849476339933 16-17 25.32525065270823 25.133362137538366 27.556663331543056 21.984723878210342 18-19 20.007060264523055 25.293925604103514 32.12434626916102 22.5746678622124 20-21 24.45672967253576 25.727035067502484 31.776840848396088 18.039394411565667 22-23 19.707650219423098 34.88902897823215 27.194984864928728 18.20833593741602 24-25 19.96363325041499 25.053770089466198 31.878704571289955 23.103892088828854 26-27 20.024924959718824 25.134731954422385 26.919498715511292 27.920844370347496 28-29 24.17273999715002 30.047798999158516 26.828320279168683 18.951140724522787 30-31 19.00775030977838 26.33291268539853 31.17164813894205 23.487688865881037 32-33 18.677824205691703 30.90396308952305 27.23407145535624 23.184141249429004 34-35 19.042052807582394 30.845346342027675 32.35270615886794 17.759894691521993 36-37 18.722695221260626 26.94413639418916 32.3671367992364 21.966031585313814 38-39 24.042708228297247 31.06305399600086 26.82162541641854 18.07261235928335 40-41 19.340235307905346 35.09824726222119 26.742326219227397 18.819191210646064 42-43 19.477597500997398 30.831667198421968 31.431171362760423 18.259563937820207 44-45 19.13031326906662 26.376760942880733 31.5689270616039 22.923998726448744 46-47 23.989967140263026 26.1789927700588 26.942577602507246 22.888462487170926 48-49 19.878121171071758 31.060332493664433 30.82780001674264 18.233746318521167 50-51 24.230694263664205 26.32846484733174 30.541395163362395 18.89944572564166 52-53 19.27264030894351 26.180947817552784 26.486677924048912 28.05973394945479 54-55 24.079672034927384 26.332886070759443 30.93903736600554 18.64840452830763 56-57 19.58695275582836 25.949072486370472 30.900431995255673 23.563542762545495 58-59 19.494782621269525 25.866836025674782 31.38291739410369 23.255463958952003 60-61 23.791225720848725 26.42738307656554 30.888123151128738 18.893268051456992 62-63 19.200481875585805 26.561270334239357 30.771669680450163 23.466578109724676 64-65 24.11463924053282 26.298309052366903 30.85159431716179 18.735457389938485 66-67 19.919372200628434 35.09792264879902 26.12633902014658 18.856366130425968 68-69 19.865479669975116 35.1315516966969 26.20460823600963 18.798360397318344 70-71 19.464186790953466 35.73700597266564 26.337913350540703 18.460893885840186 72-73 19.426769406924826 35.872209272613354 26.00611357683249 18.694907743629326 74-75 20.329382834815025 34.71440955243328 25.73061517116248 19.22559244158922 76-77 20.785416757437275 33.304093305780924 26.2905773162963 19.619912620485497 78-79 20.46600997462109 32.657161736466115 27.107592002114178 19.769236286798613 80-81 19.842753534953804 32.86981933303907 26.91854005266282 20.368887079344304 82-83 19.014428167087978 32.755641600349456 27.290433712563335 20.93949651999923 84-85 19.261825140934825 32.11970808307901 27.571771597972887 21.046695178013273 86-87 19.18254172249865 32.05593427975168 28.071418773283675 20.690105224465988 88-89 19.45374744538088 32.45658871742828 27.74548245164667 20.344181385544168 90-91 19.91804335283074 31.636778414055637 27.44767593685295 20.997502296260677 92-93 19.559077902465376 32.08488553313318 26.950548859878765 21.405487704522677 94-95 19.245672350610953 32.376706229760224 26.843841209801095 21.533780209827732 96-97 19.875868519838793 32.65900023156089 26.578509893282202 20.88662135531811 98-99 20.253111450948122 31.650699348099593 26.682442926012516 21.41374627493977 100-101 20.142724472871922 31.718565293026302 26.323706555302522 21.815003678799254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 66.0 1 37.0 2 25.0 3 74.0 4 81.5 5 58.5 6 62.5 7 56.0 8 55.5 9 68.0 10 63.0 11 61.0 12 70.5 13 81.0 14 84.5 15 83.0 16 98.5 17 121.0 18 134.5 19 160.5 20 197.0 21 265.0 22 359.0 23 467.5 24 656.5 25 932.0 26 1310.5 27 1848.0 28 2519.0 29 3358.5 30 4914.0 31 8199.5 32 18324.5 33 39819.5 34 75149.5 35 125118.0 36 194828.0 37 287512.5 38 369005.0 39 394900.5 40 398471.0 41 377629.0 42 345138.5 43 355295.0 44 350240.0 45 324972.0 46 325657.5 47 298927.5 48 230592.5 49 178382.5 50 143516.5 51 114780.5 52 87201.5 53 60355.5 54 42355.0 55 29866.0 56 21279.0 57 14468.5 58 8727.0 59 5538.0 60 3801.5 61 2642.5 62 1738.0 63 986.0 64 632.0 65 465.5 66 369.5 67 271.0 68 197.0 69 159.5 70 112.5 71 70.0 72 48.5 73 27.5 74 17.5 75 16.5 76 10.5 77 6.0 78 6.0 79 4.5 80 2.0 81 1.0 82 1.5 83 1.5 84 1.0 85 0.5 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1510223114632555 2 0.0 3 0.005022661908075013 4 0.006221251681592915 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.9025234500284142E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.4268925875213107E-4 24-25 3.8050469000568284E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.941860215133546E-4 40-41 0.0 42-43 0.0 44-45 1.617144932524152E-4 46-47 9.227238732637808E-4 48-49 0.0026064571265389273 50-51 4.851434797572456E-4 52-53 0.0013412790322700318 54-55 0.0024637678677867964 56-57 0.0041189632693115165 58-59 0.005346090894579844 60-61 0.001873985598277988 62-63 9.227238732637808E-4 64-65 0.001350791649520174 66-67 0.008019136341869766 68-69 0.007448379306861242 70-71 0.009198700880887383 72-73 0.006354428323094903 74-75 0.008922834980633263 76-77 0.0048324095630721715 78-79 0.004528005811067626 80-81 0.0018454477465275616 82-83 0.0 84-85 4.5660562800681935E-4 86-87 0.0015220187600227314 88-89 0.004879972649322882 90-91 0.006649319457849308 92-93 0.008827708808131843 94-95 0.008314027476624169 96-97 0.010273626630153437 98-99 0.007410328837860674 100-101 0.0073437405171096785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5256177.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.22766435353792 #Duplication Level Percentage of deduplicated Percentage of total 1 79.06540887801218 16.783739616376796 2 7.784327756011661 3.304861936450891 3 3.3226636214989407 2.115971643506708 4 1.9027374101988779 1.6156268438648709 5 1.3037298352494173 1.383756967518396 6 0.8192453795318931 1.0434399563933887 7 0.6726705343141132 0.9995457026054503 8 0.6051490600057474 1.0276720903728787 9 0.3826131312437213 0.7309784814568083 >10 3.2218634525674297 13.97440928213038 >50 0.39639771253158235 5.914276317001708 >100 0.4133875666420537 19.102195692642475 >500 0.07490093270097169 11.050338236362144 >1k 0.034356419018220244 11.859790147676517 >5k 4.5692561272569994E-4 0.6684477999599092 >10k+ 9.138486064190374E-5 8.424949285680668 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 434301 8.262678368707903 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8644 0.16445412702045611 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 7402 0.14082478577110322 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7031 0.1337664237714978 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6183 0.11763302491525686 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005479267536081833 0.0 0.0 2 0.005821721757086947 0.0 0.0 3 0.006278327385093767 0.0 0.0 4 0.006868109654602575 0.0 0.0 5 0.007819371379616783 0.0 0.0 6 0.00886575927713241 0.0 0.0 7 0.009817021002146617 0.0 0.0 8 0.01097756030666395 0.0 0.0 9 0.012328351956184124 0.0 0.0 10-11 0.018949133562283005 0.0 0.0 12-13 0.024514014653616117 0.0 0.0 14-15 0.029993282189697948 0.0 0.0 16-17 0.03406468237275875 0.0 0.0 18-19 0.039772252722844 0.0 0.0 20-21 0.0460125296389372 0.0 0.0 22-23 0.05316601781104403 0.0 0.0 24-25 0.06091880086990982 0.0 0.0 26-27 0.06873817224952661 0.0 0.0 28-29 0.07781320910616214 0.0 0.0 30-31 0.12001117922779236 0.0 0.0 32-33 0.1317116984454671 0.0 0.0 34-35 0.14234680453112597 0.0 0.0 36-37 0.1531911881962879 0.0 0.0 38-39 0.16584296913897686 0.0 0.0 40-41 0.17870402766116894 0.0 0.0 42-43 0.19206925489761856 0.0 0.0 44-45 0.20716577847359402 0.0 0.0 46-47 0.2227189076775763 0.0 0.0 48-49 0.2394516014205762 0.0 0.0 50-51 0.2581534069343555 9.512617250142071E-6 0.0 52-53 0.2779681886664015 1.9025234500284142E-5 0.0 54-55 0.2991147368134673 1.9025234500284142E-5 0.0 56-57 0.32106985742679517 1.9025234500284142E-5 0.0 58-59 0.3434815836681299 1.9025234500284142E-5 0.0 60-61 0.368062186642497 1.9025234500284142E-5 0.0 62-63 0.39463092662214383 1.9025234500284142E-5 0.0 64-65 0.42332098024857234 1.9025234500284142E-5 0.0 66-67 0.45489335690179383 1.9025234500284142E-5 0.0 68-69 0.48988076314781637 1.9025234500284142E-5 0.0 70-71 0.5260477339328565 1.9025234500284142E-5 0.0 72-73 0.5648306744616858 1.9025234500284142E-5 0.0 74-75 0.608236746974084 1.9025234500284142E-5 0.0 76-77 0.6543729406372731 1.9025234500284142E-5 0.0 78-79 0.7050466527287799 1.9025234500284142E-5 0.0 80-81 0.760020067817351 1.9025234500284142E-5 0.0 82-83 0.8190648830889827 1.9025234500284142E-5 0.0 84-85 0.8843880257457084 1.9025234500284142E-5 0.0 86-87 0.9536113414749923 1.9025234500284142E-5 0.0 88-89 1.0302449860421365 1.9025234500284142E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 55950 0.0 82.741554 3 GATCGGA 56115 0.0 82.57928 1 CGGAAGA 56005 0.0 82.40194 4 GAAGAGC 56090 0.0 82.257774 6 ATCGGAA 56820 0.0 81.48302 2 GGAAGAG 57340 0.0 80.52255 5 TCGCCGG 150 0.0 63.33388 2 GGCGCCG 105 0.0 58.898 1 AAGAGCA 79940 0.0 57.68656 7 GAGCACA 80185 0.0 57.611004 9 AGAGCAC 80340 0.0 57.499855 8 CGGCGCC 25 0.004992476 57.08576 1 AGTCGCC 25 0.004992476 57.08576 1 ACGGACG 50 2.8033668E-5 47.571465 1 ATGGCGA 150 0.0 47.57146 1 ACGGCGA 80 4.1745807E-9 47.57146 1 CGCGTGG 40 5.350148E-4 47.57146 1 TTGTGTT 40225 0.0 46.075428 1 CGTCTTC 50730 0.0 45.576233 52-53 GCCGTCT 50810 0.0 45.514256 50-51 >>END_MODULE