FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34042-1.3410000003584a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34042-1.3410000003584a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256177
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4343018.262678368707903TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC86440.16445412702045611No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG74020.14082478577110322No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC70310.1337664237714978No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG61830.11763302491525686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG559500.082.7415543
GATCGGA561150.082.579281
CGGAAGA560050.082.401944
GAAGAGC560900.082.2577746
ATCGGAA568200.081.483022
GGAAGAG573400.080.522555
TCGCCGG1500.063.333882
GGCGCCG1050.058.8981
AAGAGCA799400.057.686567
GAGCACA801850.057.6110049
AGAGCAC803400.057.4998558
CGGCGCC250.00499247657.085761
AGTCGCC250.00499247657.085761
ACGGACG502.8033668E-547.5714651
ATGGCGA1500.047.571461
ACGGCGA804.1745807E-947.571461
CGCGTGG405.350148E-447.571461
TTGTGTT402250.046.0754281
CGTCTTC507300.045.57623352-53
GCCGTCT508100.045.51425650-51
Produced by FastQC (version 0.11.2)