FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_34012-1.3410000003579b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_34012-1.3410000003579b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809291
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT2776299.882529079401174TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32160.11447728270229035No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG28750.10233898873416816TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA348400.085.2858661
TCGGAAG349850.084.826493
CGGAAGA349400.084.63214
ATCGGAA353200.084.0353852
GAAGAGC355850.083.2212146
GGAAGAG370350.079.962935
AGAGCAC426750.069.539588
GAGCACA427200.069.421869
AAGAGCA436400.067.8494957
TCGGACG851.2914825E-1050.29973
CGTCTTC324550.045.72928652-53
GCCGTCT324450.045.71409650-51
ATGCCGT322800.045.69720548-49
TCGTATG325400.045.57252544-45
TCTCGTA323950.045.4392742-43
TGCCGTC323900.045.30777750-51
GTATGCC326500.045.30260546-47
TATGCCG323100.045.2725448-49
CGTATGC324250.045.14819746-47
CCGTCTT325650.045.09346852-53
Produced by FastQC (version 0.11.2)