##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_34012-1.3410000003579b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2809291 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.665266787954685 33.0 33.0 33.0 33.0 33.0 2 32.68653087202429 33.0 33.0 33.0 33.0 33.0 3 32.72669260678228 33.0 33.0 33.0 33.0 33.0 4 36.65170998661228 37.0 37.0 37.0 37.0 37.0 5 36.658610660127415 37.0 37.0 37.0 37.0 37.0 6 36.54133765423376 37.0 37.0 37.0 37.0 37.0 7 36.40936520994087 37.0 37.0 37.0 37.0 37.0 8 36.36189629340642 37.0 37.0 37.0 37.0 37.0 9 36.42708284759393 37.0 37.0 37.0 37.0 37.0 10-11 36.344416794130616 37.0 37.0 37.0 37.0 37.0 12-13 36.35046654120204 37.0 37.0 37.0 37.0 37.0 14-15 38.21697058083339 40.0 37.0 40.0 37.0 40.0 16-17 38.22890401884319 40.0 37.0 40.0 37.0 40.0 18-19 38.1774342707822 40.0 37.0 40.0 37.0 40.0 20-21 38.19462828877464 40.0 37.0 40.0 37.0 40.0 22-23 38.03239482844604 40.0 37.0 40.0 35.0 40.0 24-25 38.060054120416865 40.0 37.0 40.0 35.0 40.0 26-27 37.92820092329346 40.0 37.0 40.0 33.0 40.0 28-29 37.757501803835915 40.0 37.0 40.0 33.0 40.0 30-31 37.74026364659268 40.0 37.0 40.0 33.0 40.0 32-33 37.620248489743496 40.0 37.0 40.0 33.0 40.0 34-35 37.591217855323634 40.0 37.0 40.0 33.0 40.0 36-37 37.54976397959486 40.0 37.0 40.0 33.0 40.0 38-39 37.36495631816 40.0 37.0 40.0 33.0 40.0 40-41 37.31281415844781 40.0 37.0 40.0 33.0 40.0 42-43 37.06570305461413 38.5 37.0 40.0 33.0 40.0 44-45 37.012967150786444 38.5 37.0 40.0 33.0 40.0 46-47 37.04968460013576 38.5 37.0 40.0 33.0 40.0 48-49 36.68497941295509 37.0 37.0 40.0 33.0 40.0 50-51 36.65093025250855 37.0 37.0 40.0 33.0 40.0 52-53 36.59045146978366 37.0 37.0 40.0 33.0 40.0 54-55 36.26424514227967 37.0 37.0 40.0 33.0 40.0 56-57 36.16205832717223 37.0 37.0 40.0 33.0 40.0 58-59 35.86748346824875 37.0 37.0 40.0 33.0 40.0 60-61 35.575126072735074 37.0 35.0 40.0 27.0 40.0 62-63 35.35655597800299 37.0 33.0 38.5 27.0 40.0 64-65 35.088761185651464 37.0 33.0 37.0 27.0 40.0 66-67 34.40930932395398 37.0 33.0 37.0 27.0 40.0 68-69 34.194483412362764 37.0 33.0 37.0 27.0 40.0 70-71 33.67071869735104 37.0 33.0 37.0 27.0 40.0 72-73 32.5865759367755 37.0 33.0 37.0 22.0 38.5 74-75 31.66278466702097 37.0 33.0 37.0 15.0 37.0 76-77 29.17976866761044 33.0 27.0 35.0 15.0 37.0 78-79 31.1913354650693 33.0 33.0 37.0 15.0 37.0 80-81 31.440041811261274 37.0 33.0 37.0 15.0 37.0 82-83 31.338015000937958 37.0 33.0 37.0 15.0 37.0 84-85 31.132499979532202 35.0 33.0 37.0 15.0 37.0 86-87 30.946774826815734 33.0 33.0 37.0 15.0 37.0 88-89 30.79685372572653 33.0 33.0 37.0 15.0 37.0 90-91 30.63258558832104 33.0 33.0 37.0 15.0 37.0 92-93 30.50439470314752 33.0 33.0 37.0 15.0 37.0 94-95 30.332939521039293 33.0 27.0 37.0 15.0 37.0 96-97 30.175226062376595 33.0 27.0 37.0 15.0 37.0 98-99 30.044512654616412 33.0 27.0 37.0 15.0 37.0 100-101 28.60226494870058 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13585986333537647 2306 2 0.16302463246482546 2306 3 0.09825633637159825 2306 4 0.12497037122303567 2306 5 0.12717924874520037 2306 6 0.17116656381175943 2306 7 0.15910573283510132 2306 8 0.22792552044109016 2306 9 0.19738928083429386 2306 10-11 0.1819054458746976 2306 12-13 0.1601552345317998 2306 14-15 0.2761527286594614 2306 16-17 0.3440168070574927 2306 18-19 0.4583652750457077 2306 20-21 0.32582895396375733 2306 22-23 0.31282518943487503 2306 24-25 0.36489235395447395 2306 26-27 0.400955682712997 2306 28-29 0.31460239985137406 2306 30-31 0.42137137605952546 2306 32-33 0.457757264451395 2306 34-35 0.43638884568898106 2306 36-37 0.3592448936756085 2306 38-39 0.3838220829367458 2306 40-41 0.4631894563490775 2306 42-43 0.48251864575183845 2306 44-45 0.5018758069730183 2306 46-47 0.5217027297495562 2306 48-49 0.5019940347324336 2306 50-51 0.3493442931823125 2306 52-53 0.40357645623436866 2306 54-55 0.49673645886509377 2306 56-57 0.48305017872864653 2306 58-59 0.39458651196757444 2306 60-61 0.4120804024835607 2306 62-63 0.4786126483419153 2306 64-65 0.2425516135566781 2306 66-67 0.22247630442386424 2306 68-69 0.2749767948164106 2306 70-71 0.42035568939148504 2306 72-73 -0.017131358459629098 2306 74-75 0.11465856990762546 2306 76-77 -0.12814365392984683 2306 78-79 0.23783152957594922 2306 80-81 0.22916783844061328 2306 82-83 0.29225564605167165 2306 84-85 0.38547260898961255 2306 86-87 0.4340889919463642 2306 88-89 0.19909319637771716 2306 90-91 0.2274652267305015 2306 92-93 0.18655888852756064 2306 94-95 0.2063735606597099 2306 96-97 0.339442627216588 2306 98-99 0.38461017608415915 2306 100-101 0.2785247012140708 2307 1 0.09686179983692966 2307 2 0.11731037117818488 2307 3 0.13741727119314362 2307 4 0.12533031257225957 2307 5 0.17022472874537442 2307 6 0.1354280641943575 2307 7 0.14985028134810108 2307 8 0.229476859106569 2307 9 0.19696564440070574 2307 10-11 0.21020765126356622 2307 12-13 0.17878134438658577 2307 14-15 0.31405985353175225 2307 16-17 0.3082511622195696 2307 18-19 0.24247591639964128 2307 20-21 0.3032313059795584 2307 22-23 0.3531708955739745 2307 24-25 0.33561826417432883 2307 26-27 0.36274889170554303 2307 28-29 0.4408753397001348 2307 30-31 0.48673172351834637 2307 32-33 0.4235581339534136 2307 34-35 0.4414594446365214 2307 36-37 0.43971080825580344 2307 38-39 0.32093921776940704 2307 40-41 0.4460894636162962 2307 42-43 0.2572859877003921 2307 44-45 0.37369500697985103 2307 46-47 0.2866082344582921 2307 48-49 0.36944798404140045 2307 50-51 0.24691191043042693 2307 52-53 0.3194042572150053 2307 54-55 0.19599605694484268 2307 56-57 0.32213651762924655 2307 58-59 0.2794525171743345 2307 60-61 0.27154852715494116 2307 62-63 0.16980028380083922 2307 64-65 0.15771916582725964 2307 66-67 0.26651333306954683 2307 68-69 0.23786393482163248 2307 70-71 0.2006978474151424 2307 72-73 -0.0838816694540796 2307 74-75 -0.0711290903453623 2307 76-77 -0.3537674596470133 2307 78-79 0.05327951121124741 2307 80-81 0.26463189346632277 2307 82-83 0.42863002780324777 2307 84-85 0.44736627667226614 2307 86-87 0.20201167722852276 2307 88-89 0.24935225155305218 2307 90-91 0.2227043397871391 2307 92-93 0.12954815440965106 2307 94-95 0.17630178613175573 2307 96-97 0.18810909154093025 2307 98-99 -0.01842474506645786 2307 100-101 -0.16323170142435828 2201 1 0.09571581079856628 2201 2 0.1007653828843118 2201 3 0.12298765900568753 2201 4 0.14655371406471573 2201 5 0.13045850512264678 2201 6 0.1514671013096276 2201 7 0.12305006665550877 2201 8 0.09882583652729693 2201 9 0.0731055254733235 2201 10-11 0.16484306823081596 2201 12-13 0.19469120043096666 2201 14-15 0.20541693771394165 2201 16-17 0.28720359318993616 2201 18-19 0.18295528109425163 2201 20-21 0.23421619862364196 2201 22-23 0.4308189151502404 2201 24-25 0.2268955119538134 2201 26-27 0.24808246358620067 2201 28-29 0.33162480152582674 2201 30-31 0.37655085078141326 2201 32-33 0.3534206999146363 2201 34-35 0.38126597121434713 2201 36-37 0.3035683878084541 2201 38-39 0.3410315394312349 2201 40-41 0.33276524894679227 2201 42-43 0.2960508896939942 2201 44-45 0.36781654684130416 2201 46-47 0.26094766036721495 2201 48-49 0.25018182486492435 2201 50-51 0.24033319847055168 2201 52-53 0.22244554321594023 2201 54-55 0.18334285281181195 2201 56-57 0.31713221800610825 2201 58-59 0.20858241581095172 2201 60-61 0.15842428824594634 2201 62-63 0.135073276203876 2201 64-65 0.05483437841332517 2201 66-67 0.34813345415155794 2201 68-69 0.2423257316601024 2201 70-71 0.14580185597205997 2201 72-73 0.007897585778088967 2201 74-75 0.042170788649588786 2201 76-77 -0.06598367971076868 2201 78-79 0.055241851514395535 2201 80-81 0.07387868029158895 2201 82-83 0.26094597603847447 2201 84-85 0.25996830855681097 2201 86-87 0.15766467167075504 2201 88-89 0.13827134361115512 2201 90-91 0.209298653511496 2201 92-93 -0.004693101580482306 2201 94-95 0.10170072767419569 2201 96-97 0.23812900254738523 2201 98-99 -0.004543310781436105 2201 100-101 0.10613247376941004 1101 1 0.056588339769255924 1101 2 0.0765086985049308 1101 3 0.04675086129649486 1101 4 0.07035218387545683 1101 5 0.09566788628294631 1101 6 0.10345010973435365 1101 7 0.18524144295731304 1101 8 0.058865104542022095 1101 9 0.09884923587961225 1101 10-11 0.10285119028801404 1101 12-13 0.1100857360709 1101 14-15 0.13494901141779536 1101 16-17 0.1319070170344645 1101 18-19 0.051499548411527485 1101 20-21 0.0812751769709692 1101 22-23 0.18073561537369187 1101 24-25 0.07978178999346142 1101 26-27 0.17668600139219848 1101 28-29 0.14131047317469125 1101 30-31 0.16536341444060554 1101 32-33 0.1404751513862621 1101 34-35 0.21765915668728297 1101 36-37 0.14758172630031652 1101 38-39 0.10299420323438824 1101 40-41 0.033045546773102785 1101 42-43 0.13722124497478205 1101 44-45 -0.11962163630239786 1101 46-47 0.08860187023001487 1101 48-49 0.13455752396455267 1101 50-51 0.09187325737256913 1101 52-53 0.21300884973016565 1101 54-55 0.23845756436823962 1101 56-57 0.11623447539057707 1101 58-59 0.10989810485671114 1101 60-61 0.2405926090645707 1101 62-63 0.059198891740528836 1101 64-65 -0.009351455097664996 1101 66-67 0.14047777042225107 1101 68-69 0.015413982048968933 1101 70-71 -0.058944783743903884 1101 72-73 -0.1731766523513656 1101 74-75 0.026005631380133565 1101 76-77 -0.1898092581375721 1101 78-79 0.06419269543456352 1101 80-81 0.2557540701424337 1101 82-83 0.12493941733253067 1101 84-85 0.32935294297211826 1101 86-87 0.25524147722302004 1101 88-89 0.23002663966093095 1101 90-91 0.22662237512969696 1101 92-93 0.2675808375646227 1101 94-95 0.16779811340348516 1101 96-97 0.15993775257699738 1101 98-99 0.11577472855500659 1101 100-101 0.1408720336623439 2304 1 0.05339400786355242 2304 2 0.10603227376441282 2304 3 0.08570357573103848 2304 4 0.07959322257592305 2304 5 0.05257165600938407 2304 6 0.04996398093858545 2304 7 0.1322472692489356 2304 8 0.03983271267568256 2304 9 0.10368293576771492 2304 10-11 0.04056220043580083 2304 12-13 -0.027302609416615553 2304 14-15 0.16428424504822914 2304 16-17 0.14411449668103415 2304 18-19 0.14449473496861032 2304 20-21 0.19297574995395195 2304 22-23 0.150440828168378 2304 24-25 0.10465011966125104 2304 26-27 0.1734634544153124 2304 28-29 0.1365826400921506 2304 30-31 0.02858470741432484 2304 32-33 0.18183241688767282 2304 34-35 0.06848848685666553 2304 36-37 0.1686740595178975 2304 38-39 0.16190944226669046 2304 40-41 0.2071255425287788 2304 42-43 0.30722235428527256 2304 44-45 0.2697340472257892 2304 46-47 0.16743694952160126 2304 48-49 0.2707379267889536 2304 50-51 0.10107981098603602 2304 52-53 0.19727111002263342 2304 54-55 0.07958621544668887 2304 56-57 0.04540572399187681 2304 58-59 0.18242072514454577 2304 60-61 0.1017119998119611 2304 62-63 0.26091205298605047 2304 64-65 -0.048932376886448026 2304 66-67 -0.033600537252247875 2304 68-69 -0.003109815072612321 2304 70-71 -0.0032950171814079 2304 72-73 -0.05748931392485446 2304 74-75 -0.06983879505811075 2304 76-77 -0.5047663808755445 2304 78-79 -0.06787216546754848 2304 80-81 0.1268946342509345 2304 82-83 0.20956027429900104 2304 84-85 0.20968824412580034 2304 86-87 0.17197952560825414 2304 88-89 0.12099395840924032 2304 90-91 0.10088371903524163 2304 92-93 -0.05051925118021572 2304 94-95 0.2063295310058315 2304 96-97 -0.026317890437240976 2304 98-99 -0.007981637922593876 2304 100-101 0.03107055200937836 2202 1 0.056953184535942114 2202 2 0.08201855163748206 2202 3 0.10642970494772896 2202 4 0.10680877431978075 2202 5 0.06006879723293679 2202 6 0.05717524451777223 2202 7 0.05804772665316449 2202 8 0.15034586304732045 2202 9 0.12962633199413176 2202 10-11 0.13617755206529836 2202 12-13 0.10436906010882296 2202 14-15 0.1120079443049562 2202 16-17 0.03354417078051597 2202 18-19 -0.03128070814173611 2202 20-21 -0.048926765769316205 2202 22-23 0.026809467390798147 2202 24-25 -0.11166992036162071 2202 26-27 0.04375004675378591 2202 28-29 0.050690701841730856 2202 30-31 -0.029927299446733002 2202 32-33 -0.011099258355322661 2202 34-35 -0.022077550879174623 2202 36-37 -0.022846094856035393 2202 38-39 0.07527064867034028 2202 40-41 0.008962306393847541 2202 42-43 -0.10739671375360871 2202 44-45 -0.04447407169931239 2202 46-47 -0.12174972858017696 2202 48-49 -0.25882182163871903 2202 50-51 -0.06902096088360565 2202 52-53 -0.08414326962287788 2202 54-55 -0.06501427054531206 2202 56-57 -0.06439273226884268 2202 58-59 -0.1474499152213724 2202 60-61 -0.13629900647735127 2202 62-63 -0.24753563140503587 2202 64-65 -0.3012086776297309 2202 66-67 -0.06822389345578728 2202 68-69 -0.13988440055003082 2202 70-71 -0.12803058036037385 2202 72-73 -0.35939040025989755 2202 74-75 -0.2115269025481048 2202 76-77 -0.5294018868789756 2202 78-79 -0.16577453825199484 2202 80-81 0.025643919556824102 2202 82-83 0.036497095339594665 2202 84-85 0.01670654029503993 2202 86-87 -0.046838370332284995 2202 88-89 -0.07228791694810255 2202 90-91 -0.006184605721763603 2202 92-93 -0.028281543918925678 2202 94-95 -0.01055869083522154 2202 96-97 0.0142624411808292 2202 98-99 -0.01571462820275471 2202 100-101 -0.05493573729880197 1102 1 0.030975177958879385 1102 2 0.042356177716186494 1102 3 0.01074921100612869 1102 4 -0.01757269514544646 1102 5 -0.020853039488244463 1102 6 -0.06910457847545359 1102 7 0.011203280737341004 1102 8 0.036996969891433196 1102 9 -0.10337925188544972 1102 10-11 0.033926619579482065 1102 12-13 0.013047247665383566 1102 14-15 -0.04701895984212712 1102 16-17 -0.1630479797855955 1102 18-19 -0.10794262961429268 1102 20-21 -0.11673903816553377 1102 22-23 -0.15183646939880902 1102 24-25 -0.1328625917955435 1102 26-27 -0.16442410187011092 1102 28-29 -0.1980345790075475 1102 30-31 -0.07560958663029282 1102 32-33 -0.06530965253749343 1102 34-35 -0.17123541686606814 1102 36-37 -0.11171433847209045 1102 38-39 -0.0240721971218818 1102 40-41 -0.16871086560288262 1102 42-43 -0.16250276332433344 1102 44-45 -0.34039168740479653 1102 46-47 -0.2657593348394087 1102 48-49 -0.23584597798734563 1102 50-51 -0.25588008379516936 1102 52-53 -0.3574814860920057 1102 54-55 -0.3488458492603499 1102 56-57 -0.4902572128356155 1102 58-59 -0.42019981038569654 1102 60-61 -0.40924146823991947 1102 62-63 -0.4840995591015158 1102 64-65 -0.2794149942555677 1102 66-67 -0.1628299595795184 1102 68-69 -0.2709621113801006 1102 70-71 -0.37318524058240854 1102 72-73 -0.46042316968760844 1102 74-75 -0.3560939311737634 1102 76-77 -0.4875960370478154 1102 78-79 -0.13902254085633103 1102 80-81 0.04733580196429088 1102 82-83 0.028573034998384372 1102 84-85 0.04558104646379135 1102 86-87 0.025337411521210385 1102 88-89 -0.034479755214231744 1102 90-91 0.043567788945964026 1102 92-93 -0.029200649394656608 1102 94-95 -0.048352092808340075 1102 96-97 -0.01648283017806662 1102 98-99 0.031204720050350687 1102 100-101 -0.06378497255007431 2305 1 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-0.33362385187583143 1213 88-89 -0.45008198667765953 1213 90-91 -0.3151093950901007 1213 92-93 -0.32979931378346805 1213 94-95 -0.10765494135889142 1213 96-97 -0.16823127478063427 1213 98-99 -0.21940679177394884 1213 100-101 -0.00240117508681692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 5.0 9 23.0 10 28.0 11 25.0 12 34.0 13 85.0 14 228.0 15 549.0 16 1076.0 17 1847.0 18 2789.0 19 3987.0 20 5671.0 21 7697.0 22 10545.0 23 13972.0 24 18782.0 25 25018.0 26 34482.0 27 51426.0 28 89409.0 29 141171.0 30 103269.0 31 63621.0 32 76756.0 33 109542.0 34 166368.0 35 286514.0 36 547206.0 37 815228.0 38 231722.0 39 215.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36499706236346 22.75042638510989 21.36553040026011 13.519046152266542 2 27.532142451600777 30.386528131119206 29.717426923732717 12.363902493547304 3 33.98796579332106 17.179696841652607 33.76149231851953 15.0708450465068 4 26.830091407118555 17.38568592361701 30.023555746523506 25.760666922740928 5 38.64327333836188 16.056150822396116 30.059541713549788 15.241034125692213 6 33.43202964733806 16.993647151541083 34.17673711979286 15.397586081327994 7 29.32469438018347 27.86001877342005 26.05895936020868 16.756327486187796 8 26.385910181608104 34.89994450557098 22.66401024315388 16.050135069667043 9 36.70043438006244 22.89510057875813 23.221054707397705 17.183410333781726 10-11 26.58592506080716 30.904630385389055 26.79761548376441 15.711829070039379 12-13 21.126519913045612 29.330321657899916 26.648486503168595 22.894671925885874 14-15 20.42244822626065 29.871202378108926 27.33784075768584 22.368508637944593 16-17 25.131358766322176 25.024516862083708 28.062169422818783 21.781954948775333 18-19 19.063439850125885 25.13886955819102 33.66732033100167 22.13037026068143 20-21 24.47354154482394 25.662008670515085 33.15674666668565 16.707703117975317 22-23 18.821354899912254 36.62059919160925 27.688255196606963 16.869790711871527 24-25 19.192586170778707 25.194386269415713 33.14927737092337 22.463750188882212 26-27 19.23392770631451 25.47827903908851 27.563128917580983 27.72466433701599 28-29 24.367909910365285 31.123422244260208 27.438577918770253 17.070089926604258 30-31 18.49856422848327 26.350349607783603 32.558820001203145 22.592266162529974 32-33 18.111776245323107 31.913746208562944 27.482129832758517 22.492347713355436 34-35 23.78030613418119 26.25518324730332 27.94041272335262 22.02409789516287 36-37 18.254071934876094 26.352912532023204 33.46808856754249 21.924926965558214 38-39 18.780683020977516 31.602984334539634 27.402212568071523 22.214120076411323 40-41 18.84370469274988 25.940637691146982 37.90294419481641 17.312713421286723 42-43 18.47193829332739 31.375033771866285 32.6677798775563 17.485248057250033 44-45 18.22133002193263 26.016304151466326 32.967736090808884 22.794629735792157 46-47 23.844107589975717 26.072133638135053 27.353948978698288 22.72980979319094 48-49 18.756199468712317 31.797434334355266 32.10670250114578 17.33966369578663 50-51 24.234672855694846 26.270199847113446 31.845219714356652 17.64990758283506 52-53 18.436566253008817 26.099814683370553 26.71768510126917 28.745933962351465 54-55 23.99871922062546 26.27277895307143 31.9835492112243 17.74495261507881 56-57 18.685944226290086 26.122492277976473 31.989076500450487 23.202486995282953 58-59 18.540386390504953 26.092608130568678 32.581118617990576 22.7858868609358 60-61 23.615205961298972 26.343243854639674 32.439543102820735 17.60200708124062 62-63 18.184274337656642 26.432828765358092 32.083980595656946 23.29891630132832 64-65 23.64963633564445 26.327644097495686 32.014708539015274 18.008011027844596 66-67 18.421192685747403 36.844984102352974 26.55616364671002 18.177659565189607 68-69 18.382728820944532 37.252767438350276 26.32717073393006 18.037333006775132 70-71 18.01121629340431 37.57412011262928 26.51939444760191 17.895269146364495 72-73 17.98517075594899 36.94192985931672 26.53844462920037 18.53445475553391 74-75 18.504709960353104 36.11854839025477 26.388255518686588 18.988486130705535 76-77 18.811805586827244 35.061351937223975 26.754869736040455 19.371972739908333 78-79 19.090184675972047 34.309857720990614 27.04308595779491 19.556871645242435 80-81 18.740515690158745 34.33178665442138 26.949037908761962 19.978659746657907 82-83 18.435452219083036 33.893800962591634 27.11006086589107 20.560685952434262 84-85 18.68617366068139 33.08753514686843 27.569632536131106 20.656658656319067 86-87 18.693407037190035 32.85836254772386 27.927972503795928 20.520257911290177 88-89 19.10727081858249 33.19186760744578 27.451523782367758 20.24933779160397 90-91 19.505422721677288 32.73666070272891 27.10568771701744 20.652228858576358 92-93 19.201069830162858 32.70917080686526 27.30147694375738 20.78828241921451 94-95 18.864968130871944 32.68603644662738 27.337444663556383 21.111550758944297 96-97 19.004826437807658 33.38924869371122 26.917478860416 20.688446008065124 98-99 19.411843337404058 32.655068925554495 26.891619200083728 21.041468536957723 100-101 19.524546587556742 32.55828317172092 26.429459108956046 21.4877111317663 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 178.0 1 116.0 2 86.0 3 185.0 4 194.5 5 116.5 6 95.5 7 102.0 8 115.5 9 112.5 10 103.5 11 108.0 12 114.0 13 112.0 14 110.5 15 128.5 16 152.0 17 182.5 18 233.0 19 318.0 20 471.0 21 689.0 22 1079.0 23 1778.0 24 2758.0 25 4221.0 26 6894.5 27 10914.0 28 15237.0 29 19460.5 30 24250.0 31 28829.0 32 35188.5 33 46242.0 34 62680.0 35 86822.5 36 114793.5 37 147137.5 38 184102.0 39 205886.0 40 208769.0 41 198745.5 42 184936.5 43 178546.0 44 166009.0 45 150097.0 46 143403.5 47 128077.0 48 99410.5 49 75803.5 50 61076.0 51 49000.0 52 38684.5 53 31173.5 54 25143.5 55 19341.5 56 13894.0 57 9925.5 58 7020.5 59 5171.5 60 3804.5 61 2737.0 62 1943.5 63 1296.0 64 913.0 65 650.5 66 448.5 67 303.5 68 211.5 69 145.0 70 108.0 71 84.0 72 65.5 73 47.0 74 26.0 75 18.5 76 16.5 77 12.0 78 6.5 79 3.0 80 3.5 81 5.0 82 2.5 83 0.5 84 1.0 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15359747352623848 2 0.0 3 0.004698694439273112 4 0.007475195698843588 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.119233998898654E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.1357701996695965E-4 24-25 1.7798084997246635E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.653176548816054E-4 40-41 0.0 42-43 0.0 44-45 2.3137510496420627E-4 46-47 0.001174673609818278 48-49 0.0027942993445677217 50-51 6.585291448981255E-4 52-53 0.0016018276497521973 54-55 0.0022781548796475693 56-57 0.004200348059350207 58-59 0.005837771879096896 60-61 0.0013348563747934976 62-63 8.187119098733452E-4 64-65 0.001370452544787991 66-67 0.008098128673747219 68-69 0.006941253148926188 70-71 0.008988032923609552 72-73 0.006389512514011543 74-75 0.00868546547865636 76-77 0.004609704014286879 78-79 0.004538511674297892 80-81 0.0016374238197466905 82-83 0.0 84-85 2.8476935995594616E-4 86-87 0.001085683184832045 88-89 0.0046275020992841255 90-91 0.006585291448981255 92-93 0.008792253988639838 94-95 0.00799134016376374 96-97 0.010091514193438842 98-99 0.00745739761384634 100-101 0.007261618678876629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2809291.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.82932890410179 #Duplication Level Percentage of deduplicated Percentage of total 1 90.8089641483919 45.24949741991007 2 4.294845999096722 4.280185877629124 3 1.365843534113741 2.0417720007868314 4 0.7285913704666376 1.4522087614268946 5 0.5016588835366159 1.2498662752705263 6 0.29520821831898003 0.8826016443486207 7 0.22105817436679295 0.7710626334224248 8 0.16534650004828808 0.6591284107238583 9 0.16793707510149106 0.7531372575382538 >10 1.2138353441955156 11.794393487809762 >50 0.12105100619440379 4.249321559637114 >100 0.10182036914343465 10.902873397639627 >500 0.011085715720305178 3.6592992586751305 >1k 0.0026818540847964693 2.1207932395801152 >5k 0.0 0.0 >10k+ 7.180722019070632E-5 9.93385877560162 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 277629 9.882529079401174 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3216 0.11447728270229035 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG 2875 0.10233898873416816 TruSeq Adapter, Index 2 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005375021669168484 0.0 0.0 2 0.005837771879096897 0.0 0.0 3 0.006264925919030816 0.0 0.0 4 0.006620887618975749 0.0 0.0 5 0.007937945908772 0.0 0.0 6 0.008970234838612305 0.0 0.0 7 0.010251696958414063 0.0 0.0 8 0.01174673609818278 0.0 0.0 9 0.012885813538006564 0.0 0.0 10-11 0.020147432216883192 0.0 0.0 12-13 0.02475713623117007 0.0 0.0 14-15 0.03061270619526421 0.0 0.0 16-17 0.035845343184454724 0.0 0.0 18-19 0.04193228825351308 0.0 0.0 20-21 0.04732510800767881 0.0 0.0 22-23 0.05300269712180049 0.0 0.0 24-25 0.059819363675745946 0.0 0.0 26-27 0.06649364554971343 0.0 0.0 28-29 0.07464516847845239 0.0 0.0 30-31 0.14190413168304744 0.0 0.0 32-33 0.15343729076126325 0.0 0.0 34-35 0.16238972751487832 0.0 0.0 36-37 0.17248124170831713 0.0 1.7798084997246635E-5 38-39 0.18401440078653297 0.0 5.3394254991739906E-5 40-41 0.19583232922470473 0.0 7.119233998898654E-5 42-43 0.20770365191786824 0.0 7.119233998898654E-5 44-45 0.22117680226078396 0.0 7.119233998898654E-5 46-47 0.23495252004865286 0.0 7.119233998898654E-5 48-49 0.2497071325113703 0.0 7.119233998898654E-5 50-51 0.26593898602885924 0.0 7.119233998898654E-5 52-53 0.28268698401126835 0.0 7.119233998898654E-5 54-55 0.3011969924084048 0.0 7.119233998898654E-5 56-57 0.32157579973025224 0.0 7.119233998898654E-5 58-59 0.34321827108690417 0.0 7.119233998898654E-5 60-61 0.3672278877481898 0.0 7.119233998898654E-5 62-63 0.3921452067443352 0.0 7.119233998898654E-5 64-65 0.419696642320073 0.0 1.0678850998347981E-4 66-67 0.44870752086558496 0.0 1.0678850998347981E-4 68-69 0.4818653532154554 0.0 1.2458659498072646E-4 70-71 0.5155749262002405 0.0 1.4238467997797308E-4 72-73 0.5526661353345026 0.0 1.4238467997797308E-4 74-75 0.5946340197580101 0.0 1.4238467997797308E-4 76-77 0.6389868475711488 0.0 1.601827649752197E-4 78-79 0.6877713985486018 0.0 1.7798084997246635E-4 80-81 0.7406317109904242 0.0 1.7798084997246635E-4 82-83 0.7999883244562418 0.0 1.7798084997246635E-4 84-85 0.8625841893915582 0.0 1.7798084997246635E-4 86-87 0.9333137791706163 0.0 1.7798084997246635E-4 88-89 1.009489582958832 0.0 1.7798084997246635E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 34840 0.0 85.285866 1 TCGGAAG 34985 0.0 84.82649 3 CGGAAGA 34940 0.0 84.6321 4 ATCGGAA 35320 0.0 84.035385 2 GAAGAGC 35585 0.0 83.221214 6 GGAAGAG 37035 0.0 79.96293 5 AGAGCAC 42675 0.0 69.53958 8 GAGCACA 42720 0.0 69.42186 9 AAGAGCA 43640 0.0 67.849495 7 TCGGACG 85 1.2914825E-10 50.2997 3 CGTCTTC 32455 0.0 45.729286 52-53 GCCGTCT 32445 0.0 45.714096 50-51 ATGCCGT 32280 0.0 45.697205 48-49 TCGTATG 32540 0.0 45.572525 44-45 TCTCGTA 32395 0.0 45.43927 42-43 TGCCGTC 32390 0.0 45.307777 50-51 GTATGCC 32650 0.0 45.302605 46-47 TATGCCG 32310 0.0 45.27254 48-49 CGTATGC 32425 0.0 45.148197 46-47 CCGTCTT 32565 0.0 45.093468 52-53 >>END_MODULE