##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_30749-1.34100000035870.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4863300 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.648568461744084 33.0 33.0 33.0 33.0 33.0 2 32.63811383217157 33.0 33.0 33.0 33.0 33.0 3 32.7048049678202 33.0 33.0 33.0 33.0 33.0 4 36.60262229350441 37.0 37.0 37.0 37.0 37.0 5 36.625694692904 37.0 37.0 37.0 37.0 37.0 6 36.503328809656 37.0 37.0 37.0 37.0 37.0 7 36.25023420311311 37.0 37.0 37.0 33.0 37.0 8 36.22522279110892 37.0 37.0 37.0 33.0 37.0 9 36.354962885283655 37.0 37.0 37.0 37.0 37.0 10-11 36.216160117615615 37.0 37.0 37.0 35.0 37.0 12-13 36.2953234429297 37.0 37.0 37.0 35.0 37.0 14-15 38.1563413731417 40.0 37.0 40.0 35.0 40.0 16-17 38.22949766619374 40.0 37.0 40.0 37.0 40.0 18-19 38.08170450105895 40.0 37.0 40.0 37.0 40.0 20-21 38.19997583945058 40.0 37.0 40.0 37.0 40.0 22-23 37.65637704850616 40.0 37.0 40.0 33.0 40.0 24-25 38.03704758086073 40.0 37.0 40.0 37.0 40.0 26-27 38.089474328131104 40.0 37.0 40.0 37.0 40.0 28-29 37.706099562025784 40.0 37.0 40.0 33.0 40.0 30-31 37.64072204058972 40.0 37.0 40.0 33.0 40.0 32-33 37.37804001398227 40.0 37.0 40.0 33.0 40.0 34-35 37.367279830567725 40.0 37.0 40.0 33.0 40.0 36-37 37.557375239035224 40.0 37.0 40.0 33.0 40.0 38-39 37.197072872329485 40.0 37.0 40.0 33.0 40.0 40-41 36.61642321057718 37.0 37.0 40.0 33.0 40.0 42-43 36.42190498221372 37.0 37.0 40.0 33.0 40.0 44-45 36.32787818970658 37.0 37.0 40.0 33.0 40.0 46-47 36.85981905290646 37.0 37.0 40.0 33.0 40.0 48-49 36.39845094894413 37.0 37.0 40.0 33.0 40.0 50-51 36.5651288219933 37.0 37.0 40.0 33.0 40.0 52-53 36.54636584212366 37.0 37.0 40.0 33.0 40.0 54-55 35.96506456521292 37.0 35.0 40.0 30.0 40.0 56-57 35.965928176341166 37.0 37.0 40.0 33.0 40.0 58-59 35.985011206382495 37.0 37.0 40.0 33.0 40.0 60-61 35.65827174963502 37.0 35.0 40.0 27.0 40.0 62-63 35.49010229679436 37.0 33.0 40.0 27.0 40.0 64-65 35.015991919067304 37.0 33.0 38.5 27.0 40.0 66-67 33.515139103078155 37.0 33.0 37.0 27.0 40.0 68-69 33.51019564904489 37.0 33.0 37.0 27.0 40.0 70-71 32.607070507679964 35.0 33.0 37.0 24.5 37.0 72-73 29.931919581354226 33.0 24.5 37.0 15.0 37.0 74-75 28.298135422449775 33.0 22.0 37.0 10.5 37.0 76-77 25.97889354964736 30.0 22.0 33.0 6.0 35.0 78-79 27.25784302839636 33.0 18.5 37.0 6.0 37.0 80-81 27.265044311475748 33.0 15.0 37.0 6.0 37.0 82-83 27.081936133900847 33.0 15.0 37.0 6.0 37.0 84-85 26.821636440277178 33.0 15.0 37.0 6.0 37.0 86-87 26.60965620052228 33.0 15.0 37.0 6.0 37.0 88-89 26.437825550552095 33.0 15.0 37.0 6.0 37.0 90-91 26.270907511360598 33.0 15.0 37.0 6.0 37.0 92-93 26.144998149404728 33.0 15.0 37.0 6.0 37.0 94-95 25.979292969794173 33.0 15.0 37.0 6.0 37.0 96-97 25.83252164168363 33.0 15.0 37.0 6.0 37.0 98-99 25.702226882980693 33.0 15.0 37.0 6.0 37.0 100-101 24.4730457713898 30.0 15.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16276010242221872 2306 2 0.14170479340982212 2306 3 0.10437696646140182 2306 4 0.15435146751561746 2306 5 0.08749019851684636 2306 6 0.19460583295575873 2306 7 0.20827230719898182 2306 8 0.1795688627882086 2306 9 0.21694567738778403 2306 10-11 0.23559424936675555 2306 12-13 0.22716245687443148 2306 14-15 0.3714076036118925 2306 16-17 0.2460871367162767 2306 18-19 0.34364753957642336 2306 20-21 0.32748486828340617 2306 22-23 0.2527264295147944 2306 24-25 0.31536511295961844 2306 26-27 0.34666095729036783 2306 28-29 0.32984060235888535 2306 30-31 0.44468866675120466 2306 32-33 0.393869876449088 2306 34-35 0.2671464145125313 2306 36-37 0.3872839558440546 2306 38-39 0.3609802734636105 2306 40-41 0.5193162980871477 2306 42-43 0.4385742786748068 2306 44-45 0.5335775185439147 2306 46-47 0.3939625679616299 2306 48-49 0.3185292081854456 2306 50-51 0.2752155750882892 2306 52-53 0.34167821141951293 2306 54-55 0.25706571080294793 2306 56-57 0.30086357862276003 2306 58-59 0.25266065325100584 2306 60-61 0.2834809955758999 2306 62-63 0.15934375532972211 2306 64-65 0.21283410137573355 2306 66-67 -0.005581767475433708 2306 68-69 0.16164176669114738 2306 70-71 0.07427619197461155 2306 72-73 -0.7603419492345864 2306 74-75 -0.8858696722607071 2306 76-77 -1.0708250256085314 2306 78-79 -0.6540481812599346 2306 80-81 -0.40702965449771256 2306 82-83 -0.4059114871211982 2306 84-85 -0.39881976774328365 2306 86-87 -0.4784776380773579 2306 88-89 -0.542484637788025 2306 90-91 -0.5787034353600014 2306 92-93 -0.5630165495630841 2306 94-95 -0.7084781278455345 2306 96-97 -0.6360604538515879 2306 98-99 -0.4565785604991568 2306 100-101 -0.5647357534004733 2307 1 0.153776950866785 2307 2 0.13826360183303876 2307 3 0.09343605183156001 2307 4 0.17242653524173335 2307 5 0.15217593927437179 2307 6 0.18552902298164042 2307 7 0.14119978738468575 2307 8 0.22165069809037874 2307 9 0.2407540502490022 2307 10-11 0.21985812440829733 2307 12-13 0.22615940542272028 2307 14-15 0.3902037404538845 2307 16-17 0.40845457984144673 2307 18-19 0.24866632161413804 2307 20-21 0.3844327116220754 2307 22-23 0.39612300745773865 2307 24-25 0.37576069389358935 2307 26-27 0.3719920347650145 2307 28-29 0.4061314268550973 2307 30-31 0.5040157360483661 2307 32-33 0.46559711896397005 2307 34-35 0.43706040990010564 2307 36-37 0.5118431066743909 2307 38-39 0.5263140700536368 2307 40-41 0.4059332150131354 2307 42-43 0.3223688545431642 2307 44-45 0.4758668122788663 2307 46-47 0.368459783386065 2307 48-49 0.3296005396456039 2307 50-51 0.4124206266004151 2307 52-53 0.41106953240605293 2307 54-55 0.2015126729468193 2307 56-57 0.366075015020634 2307 58-59 0.3545096517101669 2307 60-61 0.25991482539956223 2307 62-63 0.2524009473899085 2307 64-65 0.28744360619460707 2307 66-67 0.18313340015299673 2307 68-69 0.2000900581803151 2307 70-71 0.1013998714412665 2307 72-73 -0.6741897996228126 2307 74-75 -0.8779364013663233 2307 76-77 -0.7492317247031615 2307 78-79 -0.410515647547939 2307 80-81 -0.3015128715133315 2307 82-83 -0.22882758730549568 2307 84-85 -0.13426394027619182 2307 86-87 -0.3318855960217064 2307 88-89 -0.15935926642735865 2307 90-91 -0.17618472885325787 2307 92-93 -0.38808931107652356 2307 94-95 -0.3443306712254959 2307 96-97 -0.22934927425905016 2307 98-99 -0.32134537117823214 2307 100-101 -0.3489789833067185 2201 1 0.13184985127450943 2201 2 0.1398539491164854 2201 3 0.13361638609598003 2201 4 0.14457935771837072 2201 5 0.12070387491750978 2201 6 0.03891473066753548 2201 7 0.09054271838930816 2201 8 0.08461001170571336 2201 9 0.18267214058492698 2201 10-11 0.2298565177914753 2201 12-13 0.13553274351490074 2201 14-15 0.303953923062501 2201 16-17 0.2614704944995907 2201 18-19 0.2834497452603557 2201 20-21 0.28574923920114514 2201 22-23 0.43154060411487905 2201 24-25 0.29845098731808406 2201 26-27 0.263702841025669 2201 28-29 0.2690392202685743 2201 30-31 0.20541142509546972 2201 32-33 0.22018945011413393 2201 34-35 0.26831056454545177 2201 36-37 0.27640544425441504 2201 38-39 0.2753420499295771 2201 40-41 0.17272041183623799 2201 42-43 0.35372449002282025 2201 44-45 0.43145894039876964 2201 46-47 0.40096742074756975 2201 48-49 0.31698275073674864 2201 50-51 0.3822792748255637 2201 52-53 0.34636693785547124 2201 54-55 0.22015781704915582 2201 56-57 0.34338087452843524 2201 58-59 0.41618314649831945 2201 60-61 0.33429237153979585 2201 62-63 0.20992448991898272 2201 64-65 0.17084796622727083 2201 66-67 0.4130822968580432 2201 68-69 0.21590252328979176 2201 70-71 0.06538436928368441 2201 72-73 -0.500814236671399 2201 74-75 -0.29989642982445375 2201 76-77 -0.1281091706840627 2201 78-79 0.08612960772957479 2201 80-81 0.21748943977782886 2201 82-83 0.13956736181661356 2201 84-85 0.24298477248139605 2201 86-87 0.21571347783487482 2201 88-89 0.037977427243351514 2201 90-91 0.018571717866208814 2201 92-93 -0.060852308996395266 2201 94-95 0.025753174513528876 2201 96-97 0.06610383066353265 2201 98-99 -0.029063809207734437 2201 100-101 -0.0583030213110014 1101 1 0.08809470306388079 1101 2 0.08363244501404665 1101 3 0.04724227274493842 1101 4 0.09790011719092462 1101 5 0.11041217190008723 1101 6 0.17353910687030805 1101 7 0.10894142626073489 1101 8 0.003039829532873739 1101 9 0.10598381668097545 1101 10-11 0.0967062877543512 1101 12-13 0.0569366518020189 1101 14-15 0.18158449507777874 1101 16-17 0.12642109602975893 1101 18-19 0.005876187870399008 1101 20-21 0.11765236182490213 1101 22-23 0.20437217627567605 1101 24-25 0.1494852107046114 1101 26-27 0.1823540357149369 1101 28-29 0.15786092917314676 1101 30-31 0.15506321884925 1101 32-33 0.04553589082470211 1101 34-35 0.05928689662687958 1101 36-37 0.06332241415690021 1101 38-39 -0.028619644643711695 1101 40-41 -0.08592919082691708 1101 42-43 -0.026814801973038982 1101 44-45 -0.14426031751615653 1101 46-47 -0.03750107448640705 1101 48-49 -0.013218304201238595 1101 50-51 0.021621588444354245 1101 52-53 -0.012889278504225388 1101 54-55 -0.13657946698473467 1101 56-57 -0.12747979670386655 1101 58-59 -0.12155030754082219 1101 60-61 0.0031974380791908175 1101 62-63 0.023083635911127942 1101 64-65 -0.026208642324867526 1101 66-67 -0.13424593022150333 1101 68-69 -0.22281071171335753 1101 70-71 -0.35810857861901013 1101 72-73 -0.9543439685961168 1101 74-75 -0.9107285389516022 1101 76-77 -0.7734323124121296 1101 78-79 -0.5050288789263213 1101 80-81 -0.25057209644517897 1101 82-83 -0.27301990282683164 1101 84-85 -0.38677159577524733 1101 86-87 -0.4169270298844765 1101 88-89 -0.4935785545436282 1101 90-91 -0.3958585529121663 1101 92-93 -0.5737694852839113 1101 94-95 -0.4884526144952197 1101 96-97 -0.5211277850877245 1101 98-99 -0.43383134904339116 1101 100-101 -0.41077958078124155 2304 1 0.07420567175986292 2304 2 0.06335586437011642 2304 3 0.052450486276363506 2304 4 0.13394558570636406 2304 5 0.11525526384116347 2304 6 0.038416197381941686 2304 7 0.09185678428635669 2304 8 0.1605620132676009 2304 9 0.08998975894825634 2304 10-11 0.04854616108279686 2304 12-13 0.035541046230889606 2304 14-15 0.11621636798941637 2304 16-17 0.14094853585259415 2304 18-19 0.04605861564214564 2304 20-21 0.07114699114265477 2304 22-23 -0.0378747750629671 2304 24-25 0.09699136095955652 2304 26-27 0.07292124067382844 2304 28-29 0.08091356321710919 2304 30-31 0.03371162878976719 2304 32-33 0.06118703217955357 2304 34-35 0.16105802732876384 2304 36-37 0.08328919571331994 2304 38-39 0.16958792704967607 2304 40-41 0.22031837907065466 2304 42-43 -0.04433100796756406 2304 44-45 0.20594324989498602 2304 46-47 0.07987761854180775 2304 48-49 0.14339117948218671 2304 50-51 0.12116481925635014 2304 52-53 0.1801351947576464 2304 54-55 0.04666771520497548 2304 56-57 0.17548566265512733 2304 58-59 0.13822580571974896 2304 60-61 0.18256445857893056 2304 62-63 0.14257809423835255 2304 64-65 0.11348064266591251 2304 66-67 0.13595159848038207 2304 68-69 0.00866051074698504 2304 70-71 -0.27114922531239216 2304 72-73 -0.7824510286204465 2304 74-75 -0.5417575832309431 2304 76-77 -0.7789447823883293 2304 78-79 -0.40384658473226764 2304 80-81 -0.2282541258394737 2304 82-83 -0.2740942055554463 2304 84-85 -0.16315720796152178 2304 86-87 -0.18543394862683726 2304 88-89 -0.3153747961884399 2304 90-91 -0.3509198448412221 2304 92-93 -0.3356872064152654 2304 94-95 -0.46339200569688543 2304 96-97 -0.5384871342927866 2304 98-99 -0.4296410072092165 2304 100-101 -0.309660230223777 2202 1 0.03496748451043885 2202 2 0.08681020321806443 2202 3 0.04402075838851971 2202 4 0.04719269153884653 2202 5 -0.006155383197715025 2202 6 0.023918050684820003 2202 7 0.08407705738756732 2202 8 0.08533117094115994 2202 9 0.07864101130575563 2202 10-11 0.07177311297882483 2202 12-13 0.10047279360716033 2202 14-15 0.10635705370454929 2202 16-17 0.03471331411456191 2202 18-19 -2.09542083197789E-4 2202 20-21 -8.450596690536827E-4 2202 22-23 0.00906335363722377 2202 24-25 0.003160429757237182 2202 26-27 0.0975198534086843 2202 28-29 0.12927929391511128 2202 30-31 0.0470540046838579 2202 32-33 -0.0185261585063472 2202 34-35 0.023707772298813268 2202 36-37 0.011236604976190279 2202 38-39 0.09521201430477788 2202 40-41 0.05324566652543439 2202 42-43 -0.02138722669044313 2202 44-45 0.03631135532037888 2202 46-47 -0.06704540982526908 2202 48-49 -0.17934779289635827 2202 50-51 0.08404893122384038 2202 52-53 0.14474847440762773 2202 54-55 0.03411755557738161 2202 56-57 0.0789681383941101 2202 58-59 0.0367578983688972 2202 60-61 -0.020156631527790125 2202 62-63 -0.22454744742388755 2202 64-65 -0.15354021091301462 2202 66-67 0.11658427156944384 2202 68-69 -0.06508026825381563 2202 70-71 0.10165220209431425 2202 72-73 -0.45067803154387676 2202 74-75 -0.19514068151691433 2202 76-77 -0.25814956999398575 2202 78-79 -0.050135581799260365 2202 80-81 0.21286348906133412 2202 82-83 0.23580136338819102 2202 84-85 0.21346132158983622 2202 86-87 0.07938677318798426 2202 88-89 0.21059639083885529 2202 90-91 0.17439919930580317 2202 92-93 0.08890773360610993 2202 94-95 0.08780795106211414 2202 96-97 0.029781333124830667 2202 98-99 0.0518451780333784 2202 100-101 -0.019868458588693017 1102 1 0.06973044315462573 1102 2 0.053444016332250044 1102 3 0.051976011453419346 1102 4 0.04388717465396752 1102 5 0.05228295590647747 1102 6 3.22655734883881E-4 1102 7 -0.0015123961593630497 1102 8 0.01143546675798035 1102 9 0.027595052028296152 1102 10-11 0.058588228785943386 1102 12-13 0.025283439480240588 1102 14-15 -0.02873318449361051 1102 16-17 -0.07442559229181 1102 18-19 0.04073328876926752 1102 20-21 -0.05990791373714188 1102 22-23 -0.1290445197133252 1102 24-25 -0.07460366454899514 1102 26-27 -0.04847485564898335 1102 28-29 -0.061355592723600694 1102 30-31 -0.011611565983010053 1102 32-33 -0.05237754675071926 1102 34-35 -0.0027332228531378178 1102 36-37 -0.07130100473946754 1102 38-39 -0.11001394806633868 1102 40-41 -0.1246632610473597 1102 42-43 -0.24818663625195114 1102 44-45 -0.30955937619668816 1102 46-47 -0.202002614272196 1102 48-49 -0.2858529615468015 1102 50-51 -0.24357270955000132 1102 52-53 -0.26797791433252627 1102 54-55 -0.21463671968118092 1102 56-57 -0.28966838629497005 1102 58-59 -0.37043048535530687 1102 60-61 -0.33236354412676405 1102 62-63 -0.3309202433603602 1102 64-65 -0.22834418030964088 1102 66-67 -0.21397237469761166 1102 68-69 -0.06391155435471063 1102 70-71 -0.23772466450968466 1102 72-73 -0.6784615603459301 1102 74-75 -0.6159014825354419 1102 76-77 -0.5178193884863695 1102 78-79 -0.0663458683324798 1102 80-81 0.06475114709882135 1102 82-83 0.048112554617020464 1102 84-85 -0.014103569134093163 1102 86-87 -0.06429463593195095 1102 88-89 -0.06657637257458404 1102 90-91 0.03794871165860769 1102 92-93 0.0025232174787852557 1102 94-95 -0.10352118997283455 1102 96-97 -0.0190514733617988 1102 98-99 -0.09767980058206405 1102 100-101 -0.17263913021578148 2305 1 0.024609833824854377 2305 2 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0.08427710544895106 1213 92-93 0.23560750721344093 1213 94-95 0.3860631158071932 1213 96-97 0.23769469325333148 1213 98-99 0.2887675988037408 1213 100-101 0.42722488526946023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 8.0 9 50.0 10 43.0 11 29.0 12 36.0 13 108.0 14 282.0 15 705.0 16 1479.0 17 2516.0 18 4348.0 19 6754.0 20 10102.0 21 15441.0 22 22837.0 23 32841.0 24 46961.0 25 67041.0 26 98578.0 27 158546.0 28 296287.0 29 515753.0 30 401489.0 31 137795.0 32 120387.0 33 167230.0 34 255102.0 35 432196.0 36 771281.0 37 1013029.0 38 283659.0 39 386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.70888577026792 19.2379536400862 14.451533987420037 12.601626602225844 2 21.447473937449878 45.96241235375157 21.0140850862583 11.576028622540251 3 25.210587399219914 15.410469613396 45.933633610415164 13.445309376968922 4 21.343145101258397 15.670375759509966 20.96048039442287 42.02599874480876 5 52.22803446219645 14.723623876791478 19.514527172907282 13.533814488104785 6 48.916168034050955 15.571319885674336 21.80387802520922 13.708634055065492 7 22.43957806427734 44.24057738572574 18.43234018053585 14.887504369461066 8 20.83387823083092 46.98095943083914 17.87023214689614 14.3149301914338 9 49.14800649764563 17.035593115785577 18.86157547344396 14.954824913124835 10-11 31.145600723788373 34.96827257212181 20.749614459317748 13.136512244772069 12-13 16.707881843543397 34.01145005009461 20.530720154380848 28.74994795198114 14-15 16.54571998437275 34.08889025970021 20.520346266938088 28.84504348898896 16-17 30.819477599518596 19.492805244669796 20.952791412496804 28.734925743314797 18-19 15.997501696379002 19.63673842863899 35.342164785228135 29.023595089753872 20-21 30.487693541422487 19.966391133592417 34.81241132564308 14.733503999342009 22-23 15.944508815525277 48.96045787886607 20.14331847029637 14.951714835312274 24-25 15.811646746713857 19.822474378497883 34.87112037962716 29.494758495161104 26-27 15.802675138280591 19.9560175189686 20.48748791972529 43.75381942302552 28-29 30.271338391627083 34.483447453375284 20.33565685851171 14.909557296485925 30-31 15.572060123784262 20.583163695433143 34.59930499866346 29.245471182119136 32-33 15.382754508255712 34.76011144695988 20.34111611457241 29.516017930211998 34-35 15.507021980959431 34.58535356650834 35.052978430283964 14.85464602224827 36-37 15.372000493492074 20.52504472271914 34.83435116073448 29.268603623054307 38-39 29.8966198740332 34.767318378978075 20.280529615337677 15.055532131651047 40-41 15.500801924619086 48.94508872576234 20.336448502045936 15.217660847572636 42-43 15.35635268233504 34.37914584746983 34.74174942939979 15.522752040795346 44-45 15.32940115703448 20.238490570693326 34.868705270809194 29.563403001463 46-47 29.899685570898605 20.14197888103213 20.668488010661985 29.289847537407276 48-49 15.399917153118784 34.712555353344825 34.8228430791856 15.06468441435079 50-51 29.957587270567675 20.32129341501071 34.26314809520035 15.457971219221264 52-53 15.727202459762879 20.2126548327008 20.14407905193932 43.91606365559701 54-55 29.683962433404236 20.113892005547374 34.84084102176683 15.361304539281567 56-57 15.474779714870818 20.212367387334368 34.899526621646274 29.413326276148545 58-59 15.582950258736004 20.28712344992304 34.76857701881427 29.361349272526688 60-61 29.942308924078347 20.194940379650934 34.52438661914133 15.338364077129391 62-63 15.538969538272271 20.269647982482557 34.90835840478822 29.283024074456954 64-65 29.948700929379267 20.497657325991735 34.686236312125686 14.867405432503311 66-67 15.525435286284104 48.82162037891 20.254623274889372 15.398321059916523 68-69 15.489168404715429 49.06106296097706 20.046617578034834 15.403151056272682 70-71 15.512509036523337 48.75747611329813 20.19302455970501 15.536990290473524 72-73 15.533683512672752 47.57901037871024 20.410044597691318 16.477261510925693 74-75 15.685776138838891 46.15904801710501 20.597863107475423 17.557312736580673 76-77 16.100744135624 44.35482610651824 20.93353364484982 18.61089611300794 78-79 16.281693222397372 42.5449496679694 21.45308985656492 19.720267253068307 80-81 16.55431664262226 41.06959704097529 21.810994105967563 20.565092210434887 82-83 16.741492402278286 40.10330434067403 21.903306396891 21.251896860156684 84-85 17.011252280096503 39.28437406763047 22.095443384826005 21.60893026744702 86-87 17.276717973013252 38.37090243466365 22.279166444335395 22.073213147987705 88-89 17.73062165522555 37.812217932653716 22.329467718001077 22.127692694119656 90-91 17.992769497239262 37.27265361836673 22.363026677515915 22.371550206878087 92-93 18.21013451078812 36.68701213067831 22.38158011490425 22.721273243629312 94-95 18.458953131448645 36.3144237201315 22.43150801130427 22.795115137115584 96-97 18.57729565483741 36.303268591047896 22.470377162995856 22.64905859111884 98-99 18.864018237365833 35.81896132476712 22.574066164738802 22.742954273128245 100-101 18.926564651135052 36.3892650296063 22.16241670496619 22.521753614292457 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23.0 1 15.0 2 12.0 3 30.5 4 36.5 5 27.0 6 27.0 7 29.5 8 33.5 9 44.5 10 45.0 11 40.0 12 40.5 13 51.5 14 63.5 15 78.0 16 135.0 17 223.0 18 346.5 19 568.5 20 962.5 21 1564.0 22 2369.0 23 3512.0 24 5151.0 25 7255.0 26 9978.0 27 13293.5 28 17148.0 29 21741.0 30 27238.0 31 34006.5 32 44776.0 33 62581.0 34 90235.5 35 129497.5 36 181099.0 37 239946.0 38 293404.5 39 329005.0 40 344287.0 41 340209.0 42 319473.5 43 290678.5 44 257747.5 45 227801.0 46 205849.0 47 188746.0 48 173789.0 49 156199.0 50 137374.5 51 123578.5 52 111734.5 53 99098.0 54 85768.5 55 71869.0 56 58413.0 57 44315.0 58 31647.5 59 22963.0 60 16887.0 61 12275.0 62 8746.0 63 6017.5 64 3891.0 65 2402.5 66 1522.5 67 1002.5 68 717.5 69 491.0 70 331.5 71 231.0 72 161.5 73 115.0 74 86.0 75 65.0 76 49.5 77 35.0 78 25.5 79 23.5 80 16.5 81 11.5 82 8.0 83 7.5 84 6.5 85 3.5 86 1.5 87 1.0 88 0.5 89 0.5 90 1.0 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14732794604486665 2 0.0 3 0.0048526720539551335 4 0.006086402237164066 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.1405424300372174E-5 14-15 0.0 16-17 1.0281084860074436E-5 18-19 0.0 20-21 0.0 22-23 1.0281084860074435E-4 24-25 4.112433944029774E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.09394855345136E-4 40-41 0.0 42-43 0.0 44-45 1.0281084860074435E-4 46-47 8.533300433861781E-4 48-49 0.002292681923796599 50-51 4.00962309542903E-4 52-53 0.0013776653712499743 54-55 0.001984249377994366 56-57 0.003516131022145457 58-59 0.004832109884234985 60-61 0.0014290707955503465 62-63 6.888326856249871E-4 64-65 0.001110357164888039 66-67 0.007217321571772253 68-69 0.006734110583348755 70-71 0.00813233812431888 72-73 0.005510661484999897 74-75 0.008173462463759176 76-77 0.004276931301790965 78-79 0.003989060925708881 80-81 0.001562724898731314 82-83 0.0 84-85 3.906812246828285E-4 86-87 0.0012645734377891556 88-89 0.004328336726091337 90-91 0.005983591388563321 92-93 0.007844467748236795 94-95 0.007381818929533444 96-97 0.00936606830752781 98-99 0.006518207801287192 100-101 0.006477083461846895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4863300.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.18412850752377 #Duplication Level Percentage of deduplicated Percentage of total 1 91.07512295290348 45.705256741070365 2 4.473698770441079 4.490173479995323 3 1.586372917378711 2.388322270397658 4 0.7970759076945002 1.6000223912796776 5 0.4034099011577778 1.0122387160454691 6 0.28975629251886753 0.8724700211778298 7 0.25368936638801887 0.8911825834666034 8 0.13863065457988355 0.5565646867615212 9 0.09987710436742984 0.4511020896481016 >10 0.7755532381287652 7.189033989086699 >50 0.06792456966218348 2.387155554888148 >100 0.03601950262126002 3.0703085400652324 >500 0.0015658733549800104 0.5182862705264253 >1k 0.0010042926292783375 1.1638358111293046 >5k 2.1332584419191984E-4 0.7738415264375663 >10k+ 8.533032957473313E-5 26.930205328024016 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 1241565 25.52927024859663 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 16201 0.3331277116361318 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT 8900 0.18300331050932495 TruSeq Adapter, Index 27 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT 7597 0.15621080336397097 TruSeq Adapter, Index 13 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT 6813 0.14009006230337426 TruSeq Adapter, Index 13 (97% over 38bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG 6755 0.1388974564596056 TruSeq Adapter, Index 13 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT 6077 0.12495630538934467 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005263915448358111 0.0 0.0 2 0.005860218370242428 0.0 0.0 3 0.00653876997100734 0.0 0.0 4 0.00709394855345136 0.0 0.0 5 0.008594986943022227 0.0 0.0 6 0.009561408919869225 0.0 0.0 7 0.011000760800279645 0.0 0.0 8 0.01291304258425349 0.0 0.0 9 0.013879464561100487 0.0 0.0 10-11 0.022824008389365248 0.0 0.0 12-13 0.029383340530092734 0.0 0.0 14-15 0.03587070507679971 0.0 0.0 16-17 0.03939711718380523 0.0 0.0 18-19 0.04307774556371188 0.0 0.0 20-21 0.04688174696193942 0.0 0.0 22-23 0.050983899821109124 0.0 3.0843254580223306E-5 24-25 0.054849587728497115 0.0 4.112433944029774E-5 26-27 0.05897230275738696 0.0 4.112433944029774E-5 28-29 0.06457549400612753 0.0 4.112433944029774E-5 30-31 0.11311249563053893 0.0 4.112433944029774E-5 32-33 0.1216560771492608 0.0 4.112433944029774E-5 34-35 0.1278041658955853 0.0 4.112433944029774E-5 36-37 0.13376719511442847 0.0 4.112433944029774E-5 38-39 0.14095367343162052 0.0 4.112433944029774E-5 40-41 0.14921966565912034 0.0 4.112433944029774E-5 42-43 0.1583184257602862 0.0 6.168650916044661E-5 44-45 0.16811629963193717 0.0 6.168650916044661E-5 46-47 0.17953858491147986 0.0 6.168650916044661E-5 48-49 0.19127958382168486 0.0 6.168650916044661E-5 50-51 0.20361688565377417 0.0 6.168650916044661E-5 52-53 0.21829827483396047 0.0 6.168650916044661E-5 54-55 0.23287685316554602 0.0 6.168650916044661E-5 56-57 0.2492649024325047 0.0 6.168650916044661E-5 58-59 0.26659881150659015 0.0 8.224867888059549E-5 60-61 0.2847346451997615 0.0 8.224867888059549E-5 62-63 0.30405280365184134 0.0 8.224867888059549E-5 64-65 0.32512902761499396 0.0 8.224867888059549E-5 66-67 0.34910451750868754 0.0 1.0281084860074435E-4 68-69 0.3742828943310098 0.0 1.3365410318096765E-4 70-71 0.4021549153866716 0.0 1.4393518804104208E-4 72-73 0.43100363950404047 0.0 1.4393518804104208E-4 74-75 0.46161042913248207 0.0 1.4393518804104208E-4 76-77 0.4969259556268377 0.0 1.4393518804104208E-4 78-79 0.5340303908868463 0.0 1.4393518804104208E-4 80-81 0.5733555404766311 0.0 1.4393518804104208E-4 82-83 0.6172352106594288 0.0 1.4393518804104208E-4 84-85 0.6649394444101742 0.0 1.4393518804104208E-4 86-87 0.7185964262949026 0.0 1.6449735776119097E-4 88-89 0.7764480908025415 0.0 1.747784426212654E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 145460 0.0 91.752426 1 TCGGAAG 146165 0.0 91.057335 3 ATCGGAA 146385 0.0 90.9432 2 CGGAAGA 146305 0.0 90.62002 4 GAAGAGC 146915 0.0 90.115814 6 GAGCACA 147990 0.0 89.30715 9 GGAAGAG 149730 0.0 88.57069 5 AGAGCAC 149685 0.0 88.35932 8 AAGAGCA 150110 0.0 88.07435 7 TCGGACG 495 0.0 50.85819 3 TTGTGTT 24800 0.0 48.888542 1 GCGCACA 790 0.0 48.699642 9 GCCGTCT 141960 0.0 46.539806 50-51 ATGCCGT 141760 0.0 46.50949 48-49 GTATGCC 142230 0.0 46.424263 46-47 TCTCGTA 140635 0.0 46.32932 42-43 TCGTATG 143050 0.0 46.310886 44-45 AATCTCG 139865 0.0 46.205986 40-41 CGTCTTC 143200 0.0 46.1188 52-53 GTCACAT 142470 0.0 46.113636 28-29 >>END_MODULE