FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_30749-1.34100000035870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_30749-1.34100000035870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863300
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT124156525.52927024859663TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT162010.3331277116361318TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCATTCAGAAATCTCGTAT89000.18300331050932495TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGACATCTCGTAT75970.15621080336397097TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGCAATCTCGTAT68130.14009006230337426TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG67550.1388974564596056TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAACTCTCGTAT60770.12495630538934467TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1454600.091.7524261
TCGGAAG1461650.091.0573353
ATCGGAA1463850.090.94322
CGGAAGA1463050.090.620024
GAAGAGC1469150.090.1158146
GAGCACA1479900.089.307159
GGAAGAG1497300.088.570695
AGAGCAC1496850.088.359328
AAGAGCA1501100.088.074357
TCGGACG4950.050.858193
TTGTGTT248000.048.8885421
GCGCACA7900.048.6996429
GCCGTCT1419600.046.53980650-51
ATGCCGT1417600.046.5094948-49
GTATGCC1422300.046.42426346-47
TCTCGTA1406350.046.3293242-43
TCGTATG1430500.046.31088644-45
AATCTCG1398650.046.20598640-41
CGTCTTC1432000.046.118852-53
GTCACAT1424700.046.11363628-29
Produced by FastQC (version 0.11.2)