FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_30521-1.341000000356df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_30521-1.341000000356df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3032762
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT678112.2359486171351395TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC38460.12681509462331697No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA36460.12022044591695623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA130700.054.94884
GAAGAGC131800.054.523546
TCGGAAG132300.054.3596533
ATCGGAA135050.053.182392
GATCGGA136850.052.3706321
GGAAGAG154350.046.834795
GCCGTCT81150.043.54925550-51
ATGCCGT82000.043.06815348-49
TGCCGTC81900.042.83146350-51
TATGCCG82200.042.73222448-49
GTATGCC83500.042.4075546-47
GATCTCG80850.042.1798440-41
TCGATCT80950.042.07044638-39
TCGTATG84850.041.7888144-45
TCTCGTA82300.041.43874742-43
CGTATGC85450.041.30082746-47
CTCGATC81700.041.2482238-39
GAGCACA173550.041.1060569
ACTCGAT85550.040.89098436-37
CCGTCTT86500.040.66455552-53
Produced by FastQC (version 0.11.2)