##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_30484-1.34100000035806.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8284331 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68726104739176 33.0 33.0 33.0 33.0 33.0 2 32.71633134890433 33.0 33.0 33.0 33.0 33.0 3 32.74259261248736 33.0 33.0 33.0 33.0 33.0 4 36.67220998291835 37.0 37.0 37.0 37.0 37.0 5 36.66837406665668 37.0 37.0 37.0 37.0 37.0 6 36.54480090184711 37.0 37.0 37.0 37.0 37.0 7 36.46006249629572 37.0 37.0 37.0 37.0 37.0 8 36.4089210100369 37.0 37.0 37.0 37.0 37.0 9 36.42853973362484 37.0 37.0 37.0 37.0 37.0 10-11 36.36333658082952 37.0 37.0 37.0 37.0 37.0 12-13 36.35317818662726 37.0 37.0 37.0 37.0 37.0 14-15 38.18907791105884 40.0 37.0 40.0 37.0 40.0 16-17 38.17071064639981 40.0 37.0 40.0 37.0 40.0 18-19 38.1455812183265 40.0 37.0 40.0 37.0 40.0 20-21 38.09301306285323 40.0 37.0 40.0 37.0 40.0 22-23 37.92395251952149 40.0 37.0 40.0 33.0 40.0 24-25 37.940400679306514 40.0 37.0 40.0 33.0 40.0 26-27 37.848841385019504 40.0 37.0 40.0 33.0 40.0 28-29 37.676771304767996 40.0 37.0 40.0 33.0 40.0 30-31 37.63620387693345 40.0 37.0 40.0 33.0 40.0 32-33 37.46942631819033 40.0 37.0 40.0 33.0 40.0 34-35 37.35477354779764 40.0 37.0 40.0 33.0 40.0 36-37 37.18200389385697 40.0 37.0 40.0 33.0 40.0 38-39 37.134682148745625 40.0 37.0 40.0 33.0 40.0 40-41 37.12226370481817 40.0 37.0 40.0 33.0 40.0 42-43 36.88091500689676 38.5 37.0 40.0 33.0 40.0 44-45 36.743852219328275 37.0 37.0 40.0 33.0 40.0 46-47 36.73421227374908 37.0 37.0 40.0 33.0 40.0 48-49 36.465446093353826 37.0 37.0 40.0 33.0 40.0 50-51 36.3540405374918 37.0 37.0 40.0 33.0 40.0 52-53 36.20886273134185 37.0 37.0 40.0 33.0 40.0 54-55 35.952579574621055 37.0 37.0 40.0 30.0 40.0 56-57 35.77479388498601 37.0 37.0 40.0 27.0 40.0 58-59 35.56989375484876 37.0 35.0 40.0 27.0 40.0 60-61 35.211622217895446 37.0 33.0 40.0 27.0 40.0 62-63 34.94375592911486 37.0 33.0 38.5 27.0 40.0 64-65 34.66576407919963 37.0 33.0 37.0 27.0 40.0 66-67 34.2129762801607 37.0 33.0 37.0 27.0 40.0 68-69 33.9600833187375 37.0 33.0 37.0 27.0 40.0 70-71 33.53218159680003 37.0 33.0 37.0 27.0 38.5 72-73 32.92966317980293 37.0 33.0 37.0 22.0 37.0 74-75 32.364620389986825 37.0 33.0 37.0 22.0 37.0 76-77 29.713383313631482 33.0 27.0 35.0 18.5 37.0 78-79 32.03394341679491 33.0 33.0 37.0 22.0 37.0 80-81 32.40651930735264 37.0 33.0 37.0 22.0 37.0 82-83 32.33985037536525 37.0 33.0 37.0 22.0 37.0 84-85 32.145213717317674 35.0 33.0 37.0 22.0 37.0 86-87 31.96548622936481 33.0 33.0 37.0 22.0 37.0 88-89 31.811622205824463 33.0 33.0 37.0 22.0 37.0 90-91 31.64807019420156 33.0 33.0 37.0 15.0 37.0 92-93 31.52765968670252 33.0 33.0 37.0 15.0 37.0 94-95 31.334783339777225 33.0 33.0 37.0 15.0 37.0 96-97 31.168349381501052 33.0 33.0 37.0 15.0 37.0 98-99 31.032439191529164 33.0 33.0 37.0 15.0 37.0 100-101 29.389975907529525 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13122634696407687 2306 2 0.13964353170576516 2306 3 0.1035401947421235 2306 4 0.15282386064974673 2306 5 0.11513263030555265 2306 6 0.12472351746510668 2306 7 0.16383768741918203 2306 8 0.1789560949351312 2306 9 0.21331946759570997 2306 10-11 0.23277644357717264 2306 12-13 0.2053728612018304 2306 14-15 0.39421281817203635 2306 16-17 0.2988916868713005 2306 18-19 0.3522579944814268 2306 20-21 0.32545584412454076 2306 22-23 0.3903367123260608 2306 24-25 0.3558224420511067 2306 26-27 0.34789942379513406 2306 28-29 0.3440203810336513 2306 30-31 0.40811221551090426 2306 32-33 0.45295027434577406 2306 34-35 0.4168579556192924 2306 36-37 0.3879294662255717 2306 38-39 0.4212358548626085 2306 40-41 0.4178878780634392 2306 42-43 0.48400514896719926 2306 44-45 0.48502702700846356 2306 46-47 0.39976274300845205 2306 48-49 0.41068334341687773 2306 50-51 0.4199753456978428 2306 52-53 0.45982073911959276 2306 54-55 0.46520157793577965 2306 56-57 0.4582070777776792 2306 58-59 0.45723599242820256 2306 60-61 0.43025645759495745 2306 62-63 0.32243893720423955 2306 64-65 0.32216749945106216 2306 66-67 0.3124644382977877 2306 68-69 0.39851878643143124 2306 70-71 0.4946206031267124 2306 72-73 0.3638543924592952 2306 74-75 0.35573078671386327 2306 76-77 0.023169765990473934 2306 78-79 0.2843863904083648 2306 80-81 0.4406087173357207 2306 82-83 0.4297238861126189 2306 84-85 0.5724933750233134 2306 86-87 0.5050545147534464 2306 88-89 0.2924197889342217 2306 90-91 0.3894711046013768 2306 92-93 0.36411322234440746 2306 94-95 0.32507445176987915 2306 96-97 0.4353252587692964 2306 98-99 0.4507007926960469 2306 100-101 0.23610324737976995 2307 1 0.08952555433747733 2307 2 0.10297578351807601 2307 3 0.09448312831595729 2307 4 0.1157981106729622 2307 5 0.10812535173018034 2307 6 0.12216559804269878 2307 7 0.1740429381120876 2307 8 0.19351776729658354 2307 9 0.1912235042965449 2307 10-11 0.19114177954526212 2307 12-13 0.18220961385247136 2307 14-15 0.33035579952832705 2307 16-17 0.3526332913352377 2307 18-19 0.27981091874318054 2307 20-21 0.2814766319362221 2307 22-23 0.32046958359939737 2307 24-25 0.28806017014816376 2307 26-27 0.36542009490031546 2307 28-29 0.32060468712487733 2307 30-31 0.33498462169354326 2307 32-33 0.3311556246363878 2307 34-35 0.33095668199080563 2307 36-37 0.35128201947062365 2307 38-39 0.29904730542059355 2307 40-41 0.29831859533796035 2307 42-43 0.18254356801565308 2307 44-45 0.3254962299335773 2307 46-47 0.23240379595776517 2307 48-49 0.280147086188407 2307 50-51 0.31307773152099827 2307 52-53 0.3175075275765238 2307 54-55 0.22207620215304047 2307 56-57 0.2394138322125272 2307 58-59 0.14866874198458646 2307 60-61 0.1925929921083096 2307 62-63 0.1657154662427729 2307 64-65 0.143100109692881 2307 66-67 0.21226987144390108 2307 68-69 0.18241510644921988 2307 70-71 0.22704185373163455 2307 72-73 0.10528457569461835 2307 74-75 0.09194406693656987 2307 76-77 -0.3066174004488893 2307 78-79 0.09955024695345571 2307 80-81 0.25374434007186153 2307 82-83 0.27961037223247587 2307 84-85 0.445764575890486 2307 86-87 0.26972536269037306 2307 88-89 0.2092903406443689 2307 90-91 0.22447214946067007 2307 92-93 0.21389371023870396 2307 94-95 0.17194725206610784 2307 96-97 0.2887215861194363 2307 98-99 0.2085892206655231 2307 100-101 -0.018866204234075923 2201 1 0.08610742239342528 2201 2 0.12785474160222066 2201 3 0.11672631434429093 2201 4 0.10879049893117099 2201 5 0.09913289546179982 2201 6 0.11156505201816458 2201 7 0.14589360828217934 2201 8 0.15633811680410048 2201 9 0.09909503415840959 2201 10-11 0.14338399669851043 2201 12-13 0.16458666657224086 2201 14-15 0.30858451504284545 2201 16-17 0.22960658492759478 2201 18-19 0.20945114222676864 2201 20-21 0.19122844068298406 2201 22-23 0.26764493487733887 2201 24-25 0.23998947303309137 2201 26-27 0.2532861353696063 2201 28-29 0.30556863633531606 2201 30-31 0.24355625151957128 2201 32-33 0.3202863709562749 2201 34-35 0.27936348970121117 2201 36-37 0.16598283678018788 2201 38-39 0.2789476408295002 2201 40-41 0.181199098764381 2201 42-43 0.12783920716015018 2201 44-45 0.1973354031457788 2201 46-47 0.11667354211788705 2201 48-49 0.19623389415789916 2201 50-51 0.1236230732559278 2201 52-53 0.021392730182071773 2201 54-55 0.08913579849647846 2201 56-57 0.11773433447305592 2201 58-59 0.09531123895111904 2201 60-61 0.16166157389108804 2201 62-63 0.1102096513729478 2201 64-65 0.026286200101075963 2201 66-67 0.17175581978639798 2201 68-69 0.027834499820066583 2201 70-71 0.12717598490706195 2201 72-73 0.0835728540872509 2201 74-75 0.09399752887030388 2201 76-77 -0.044695972710240284 2201 78-79 0.14094287130412653 2201 80-81 0.23434079469072344 2201 82-83 0.35562800144101203 2201 84-85 0.26092456982354406 2201 86-87 0.21555427446282494 2201 88-89 0.2232354353836179 2201 90-91 0.23106004127483715 2201 92-93 0.17245033464550374 2201 94-95 0.1501498700259738 2201 96-97 0.18875390444591034 2201 98-99 0.13564516551035055 2201 100-101 0.21218496046713042 1101 1 0.10620820505580042 1101 2 0.08890134600847688 1101 3 0.024258283743591846 1101 4 0.12573643480325813 1101 5 0.10786664252740508 1101 6 0.09505342941280759 1101 7 0.1359679670147571 1101 8 0.04606180849602737 1101 9 0.04374844548062384 1101 10-11 0.11529993878885847 1101 12-13 0.06664967012172696 1101 14-15 0.15305068537288946 1101 16-17 0.1680619098280829 1101 18-19 0.12007018881941178 1101 20-21 0.13715097869548742 1101 22-23 0.11170942218910795 1101 24-25 0.14481061076227775 1101 26-27 0.14966839307884072 1101 28-29 0.18052805462938437 1101 30-31 0.15144272090852695 1101 32-33 0.20821238806298226 1101 34-35 0.12270239307730435 1101 36-37 0.09715315702307947 1101 38-39 -0.037787251054808735 1101 40-41 0.06270143746067447 1101 42-43 0.16363192529306048 1101 44-45 -0.038520907529935755 1101 46-47 0.04042825307266895 1101 48-49 -0.09823851785716187 1101 50-51 -0.05318125761321113 1101 52-53 1.7711853301705105E-4 1101 54-55 0.04430427452694374 1101 56-57 0.07758382335982361 1101 58-59 -0.09631919670940903 1101 60-61 0.05552923544856725 1101 62-63 -0.09247240211465879 1101 64-65 -0.02832528200902118 1101 66-67 0.05288658466475482 1101 68-69 -0.11452568249257666 1101 70-71 -0.13486230663573195 1101 72-73 -0.17347805153775653 1101 74-75 -0.11353786485312867 1101 76-77 -0.18825511054905775 1101 78-79 -0.012673838671204862 1101 80-81 0.15873414329116997 1101 82-83 0.2170245990153532 1101 84-85 0.21366429676751153 1101 86-87 0.08067712454046472 1101 88-89 0.08972856472695767 1101 90-91 -0.002364736408146939 1101 92-93 -0.05419779399361602 1101 94-95 -0.0541659301783568 1101 96-97 0.004265335260438263 1101 98-99 0.0011443327997930908 1101 100-101 -0.054145170569544376 2304 1 0.034659643029755216 2304 2 0.06912417295610851 2304 3 0.048819961952659696 2304 4 0.07944166151280996 2304 5 0.09315257844538394 2304 6 0.042800827261686436 2304 7 0.027360209987548956 2304 8 0.10403307800370953 2304 9 0.05453204941455425 2304 10-11 0.03199642095136568 2304 12-13 0.055006899629297834 2304 14-15 0.09681243359205638 2304 16-17 0.09786943220755973 2304 18-19 0.08961798444059355 2304 20-21 0.10139718388203534 2304 22-23 0.08690658485896563 2304 24-25 0.0544232243788656 2304 26-27 0.01463751884599418 2304 28-29 0.11211126723440401 2304 30-31 0.0045918408052401105 2304 32-33 0.056184449817060056 2304 34-35 0.06986453165632156 2304 36-37 0.04221308000686719 2304 38-39 0.04202132963663274 2304 40-41 0.043519766777947666 2304 42-43 0.04428561696997946 2304 44-45 0.05300063651407072 2304 46-47 0.028525261092973153 2304 48-49 0.04915399271129672 2304 50-51 -0.09466088145259022 2304 52-53 -0.09735400115170023 2304 54-55 -0.015821446134630435 2304 56-57 -0.1124249551042027 2304 58-59 -0.07173902991774384 2304 60-61 -0.028314000242097848 2304 62-63 0.02662292901245422 2304 64-65 -0.027281357131997197 2304 66-67 -0.009179927139847166 2304 68-69 -0.0801694970161364 2304 70-71 0.015053746698768578 2304 72-73 -0.11535840584189572 2304 74-75 -0.030545550238151975 2304 76-77 -0.36182887791100526 2304 78-79 0.022076153614591476 2304 80-81 0.1269872281005533 2304 82-83 0.18958520480162377 2304 84-85 0.3070456859647521 2304 86-87 0.23108826811497352 2304 88-89 0.17040017970337473 2304 90-91 0.11520445785602362 2304 92-93 0.0568680944694826 2304 94-95 0.11695646364341172 2304 96-97 0.1303256342212471 2304 98-99 0.06551904851127333 2304 100-101 0.15901532979355792 2202 1 0.08182506229586295 2202 2 0.07865464634316766 2202 3 0.060904158438809475 2202 4 0.06856037464307718 2202 5 0.04400080968512299 2202 6 0.055391992341448315 2202 7 0.07300905848778427 2202 8 0.06358071462769743 2202 9 0.10804672555071448 2202 10-11 0.12016808731629425 2202 12-13 0.096087954256312 2202 14-15 0.08474383745585357 2202 16-17 0.019147585135257827 2202 18-19 0.013984721459991079 2202 20-21 0.04925029053058694 2202 22-23 0.08082453032653802 2202 24-25 0.04993582792155138 2202 26-27 0.11423617061070956 2202 28-29 0.03139585514959009 2202 30-31 0.07244517789406046 2202 32-33 -0.01082461458517514 2202 34-35 -0.025263978151471633 2202 36-37 0.00351457242702935 2202 38-39 0.04764045020127128 2202 40-41 -0.021631886305868875 2202 42-43 0.014650142675421307 2202 44-45 0.011951410023335995 2202 46-47 -0.11409604717119493 2202 48-49 -0.16558351243511282 2202 50-51 0.06678328999998939 2202 52-53 0.03317081857727544 2202 54-55 -0.01662062896148342 2202 56-57 0.005658314601383552 2202 58-59 -0.05915653486793104 2202 60-61 -0.0706733138905804 2202 62-63 -0.26686428708541854 2202 64-65 -0.15780249780220856 2202 66-67 0.019304486599004633 2202 68-69 -0.12817779149690267 2202 70-71 -0.07748114076024848 2202 72-73 -0.20430027888095026 2202 74-75 -0.02733030955754856 2202 76-77 -0.4354348369138563 2202 78-79 -0.11839612801444588 2202 80-81 0.06677632277717294 2202 82-83 0.17739662571800352 2202 84-85 0.15839876392235652 2202 86-87 0.1212248712816617 2202 88-89 0.1508052560515445 2202 90-91 0.13951904350445332 2202 92-93 0.1094449243391935 2202 94-95 0.1251946747698227 2202 96-97 0.1473992333069667 2202 98-99 0.02670186989644563 2202 100-101 -0.016910187348063488 1102 1 0.06596429962097972 1102 2 0.08325303249795013 1102 3 0.027084906499695194 1102 4 0.0036347747217249093 1102 5 0.023020094862445717 1102 6 -3.875650136109243E-5 1102 7 6.596725028273909E-4 1102 8 -0.036129583781594476 1102 9 0.007567129981609355 1102 10-11 0.0383579253341324 1102 12-13 -0.011897964983468512 1102 14-15 -0.044786282303981295 1102 16-17 -0.08397644300428908 1102 18-19 -0.06924562160955361 1102 20-21 -0.15698923641159723 1102 22-23 -0.11718391599937661 1102 24-25 7.374122987187093E-4 1102 26-27 -0.07699840145453862 1102 28-29 -0.1308827170512501 1102 30-31 -0.10144264179863427 1102 32-33 -0.017047784473923855 1102 34-35 -0.04109145189797658 1102 36-37 -0.0124859395022483 1102 38-39 -0.03981754450513364 1102 40-41 -0.03366432923221652 1102 42-43 -0.10465828955891254 1102 44-45 -0.15560437872941435 1102 46-47 -0.10791900670908205 1102 48-49 -0.22634492777755355 1102 50-51 -0.1927466632306718 1102 52-53 -0.14162951336524543 1102 54-55 -0.16357689408912535 1102 56-57 -0.2510586819156302 1102 58-59 -0.22075133151594883 1102 60-61 -0.13127211391685734 1102 62-63 -0.14472426715771292 1102 64-65 -0.1251576992057224 1102 66-67 -0.08424509543390712 1102 68-69 -0.1077942655096038 1102 70-71 -0.10539036528459889 1102 72-73 -0.1396058671013023 1102 74-75 -0.2895641133062128 1102 76-77 -0.400282354094891 1102 78-79 -0.07335117823989634 1102 80-81 0.033038594054204395 1102 82-83 0.08139117032369114 1102 84-85 0.08211591657101991 1102 86-87 0.027886131244777346 1102 88-89 0.027167521757885282 1102 90-91 0.12002316873642371 1102 92-93 -0.06544770872289973 1102 94-95 -0.11411352246395268 1102 96-97 -0.017094058568208226 1102 98-99 -0.11297205752675765 1102 100-101 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0.25292783113894046 1213 74-75 0.31580319784639244 1213 76-77 0.36301225849262764 1213 78-79 -0.21179003274791341 1213 80-81 -0.3196963682059675 1213 82-83 -0.23875767095712064 1213 84-85 -0.23706467997592284 1213 86-87 -0.21547134115110111 1213 88-89 -0.3690372686266912 1213 90-91 -0.2383190000343589 1213 92-93 -0.17055742281235808 1213 94-95 0.0297209710632913 1213 96-97 -0.12905379815942553 1213 98-99 -0.13902004214780206 1213 100-101 0.17756106582514875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 26.0 10 46.0 11 62.0 12 130.0 13 371.0 14 1147.0 15 2794.0 16 5056.0 17 8473.0 18 12344.0 19 16912.0 20 21395.0 21 27504.0 22 34846.0 23 43625.0 24 54617.0 25 68176.0 26 87119.0 27 117323.0 28 173672.0 29 249497.0 30 233325.0 31 217583.0 32 279055.0 33 391484.0 34 589361.0 35 977195.0 36 1737889.0 37 2367054.0 38 565655.0 39 592.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.60839215959349 28.276704897691147 16.879159304317216 17.235743638398144 2 30.60873593776009 28.11068268518001 25.093456550685865 16.18712482637403 3 33.16177932498564 21.34759749745379 26.118029431366907 19.372593746193665 4 30.135879832781853 21.02419303241632 24.587984485362377 24.25194264943945 5 34.61796734099591 19.879167068529735 25.07679859725547 20.426066993218885 6 32.44386299871408 20.207256325224087 26.45972257747789 20.88915809858394 7 30.441311434803847 24.339044395980796 22.27347024159223 22.94617392762312 8 28.667215252505002 27.121079541606917 21.827326793195493 22.384378412692588 9 29.763936279223994 22.67287485253788 25.51432336539909 22.048865502839035 10-11 24.013562471127724 29.80577429849194 28.394199845467305 17.786463384913038 12-13 21.415161424699917 29.30332180272459 28.745159383496087 20.536357389079402 14-15 21.56177728774961 29.348121169953252 28.85046481122012 20.239636731077017 16-17 23.89118083282766 26.859030620577567 28.83620294746794 20.413585599126833 18-19 21.380748789491875 26.677754667214526 31.200865827306995 20.7406307159866 20-21 23.552203551499815 27.142288254778812 30.701664383038292 18.60384381068308 22-23 21.31013948754298 31.56964285800507 28.149321537882876 18.970896116569076 24-25 21.30241712438336 26.67018535904091 30.635860303687895 21.39153721288783 26-27 21.286776204379088 26.915432278116363 28.609123657661673 23.188667859842877 28-29 23.074059933143666 29.605921105759776 28.47808109067588 18.84193787042068 30-31 20.602369702514302 27.907153878810494 30.67247675159286 20.81799966708235 32-33 20.350086204909 30.240474457140838 28.53957066659939 20.86986867135077 34-35 22.632817302930075 30.31280980926523 28.642898261790844 18.411474626013856 36-37 22.488110385739056 29.96524402513613 29.02114244348759 18.525503145637227 38-39 20.581636847763743 27.448811501958804 33.09183314354568 18.87771850673177 40-41 20.569826338421294 27.432559128793866 28.233215210739402 23.76439932204544 42-43 20.639850097732694 29.477256521981076 30.452857327888033 19.430036052398197 44-45 20.482253191435557 27.17967031816309 30.701799863971946 21.63627662642941 46-47 22.726162735675963 27.340982702610834 28.377026253172282 21.555828308540924 48-49 20.45030403975609 29.971611930980185 30.322452105318686 19.255631923945035 50-51 22.884611171362305 27.901113172105035 29.704349740656976 19.509925915875677 52-53 20.540739061957268 27.773111944489624 27.545118171993916 24.141030821559188 54-55 22.875298047200477 27.693497176680843 29.808715637998308 19.62248913812038 56-57 20.699596239373708 27.43699973171149 30.00057641288304 21.862827616031762 58-59 20.737824323316996 27.65239608359969 30.075824952852727 21.533954640230593 60-61 22.548763450526884 27.82719010395872 29.92700020635664 19.69704623915775 62-63 20.197737649333654 27.866654828956044 29.8756213515523 22.059986170158 64-65 22.394443894536842 27.624248269601726 29.99343329848797 19.987874537373465 66-67 20.60272213409383 31.535677149461694 27.835892349593948 20.025708366850527 68-69 20.49362290821702 31.903355393585215 27.7447268010482 19.85829489714957 70-71 20.294534824785515 32.29741955932348 27.695618495547443 19.712427120343566 72-73 20.294920469423158 32.114307319208095 27.557614829330383 20.03315738203836 74-75 20.557594385063535 31.627911468657423 27.537085235681985 20.277408910597057 76-77 20.73820460046234 31.033492476415237 27.69024438824474 20.538058534877685 78-79 20.623997158848105 30.663098004771626 28.009935822994887 20.70296901338538 80-81 20.351011183302923 30.83073817621429 27.81613703371142 21.002113606771363 82-83 20.167572976019425 30.68787328753523 27.78548442837448 21.359069308070865 84-85 20.391770305565 30.456841639956483 27.957521541065727 21.193866513412793 86-87 20.378107084148237 30.184185246783823 28.497428965499928 20.940278703568016 88-89 20.62932202664515 30.49908035982608 28.15271682404033 20.718880789488438 90-91 20.801711871570035 30.18316233892203 27.760704961576206 21.254420827931725 92-93 20.572999001524334 30.303579579331615 27.590945367288988 21.532476051855063 94-95 20.198634943841746 30.27731547365645 27.569071434784153 21.95497814771765 96-97 20.10970112055196 30.651015324789366 27.64898973345468 21.59029382120399 98-99 20.43282012119765 30.37135453828442 27.546646645118027 21.649178695399897 100-101 20.62445359917738 30.371236277658653 27.138607689219164 21.865702433944804 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 36.0 1 27.5 2 24.0 3 41.5 4 43.0 5 30.0 6 36.0 7 43.0 8 38.5 9 45.0 10 58.5 11 56.5 12 55.0 13 65.5 14 89.0 15 119.0 16 156.5 17 208.5 18 300.5 19 508.5 20 818.0 21 1311.0 22 2125.5 23 3482.0 24 5342.0 25 7608.5 26 11041.5 27 16009.0 28 21790.5 29 28389.5 30 35943.5 31 44710.0 32 61076.5 33 89714.5 34 131719.5 35 193429.0 36 268621.5 37 355723.5 38 446820.0 39 503472.0 40 548024.0 41 560366.0 42 529407.5 43 520326.5 44 508929.0 45 495819.0 46 506611.5 47 479209.0 48 397117.0 49 316910.0 50 256980.5 51 210702.0 52 169920.0 53 134612.0 54 109043.0 55 87707.0 56 65432.5 57 45591.5 58 32157.0 59 23321.5 60 16952.0 61 12034.0 62 8137.0 63 5338.0 64 3560.5 65 2493.5 66 1739.0 67 1248.5 68 931.0 69 665.0 70 484.5 71 354.0 72 251.5 73 190.0 74 153.5 75 114.0 76 86.5 77 68.5 78 50.0 79 38.5 80 30.0 81 23.5 82 18.5 83 16.0 84 15.0 85 11.0 86 7.5 87 5.5 88 4.0 89 4.0 90 2.5 91 1.0 92 2.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15106832404451245 2 0.0 3 0.005407799374505919 4 0.006530400583945765 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.828392298666E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.449686522665498E-5 24-25 4.224843261332749E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.751461041332124E-4 40-41 0.0 42-43 0.0 44-45 1.267452978399825E-4 46-47 9.053235559998749E-4 48-49 0.002619402822026305 50-51 3.923068742666125E-4 52-53 0.0013700563147464775 54-55 0.0023960896782130024 56-57 0.004086026982746103 58-59 0.005413834864879253 60-61 0.0015631920066931176 62-63 8.630751233865474E-4 64-65 0.001339878862879815 66-67 0.007803889052718923 68-69 0.006983062361945702 70-71 0.008769567512452121 72-73 0.006089809786692492 74-75 0.008582467310878814 76-77 0.0045990436644793645 78-79 0.0044300499340260546 80-81 0.0018166826023730826 82-83 0.0 84-85 4.5869726837327E-4 86-87 0.001412304747359805 88-89 0.004641292097092692 90-91 0.006421761757225779 92-93 0.00849797044565216 94-95 0.007755605129732262 96-97 0.009946488135251959 98-99 0.0071158431501590165 100-101 0.006964955890825705 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8284331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.35199240234738 #Duplication Level Percentage of deduplicated Percentage of total 1 88.40232781042663 40.976240270187176 2 5.544639901948935 5.140102132177784 3 1.8652246545766742 2.593706370528271 4 0.8770501313209109 1.6261208409385857 5 0.5818110287519276 1.348405019215563 6 0.42095882612240737 1.170736818607614 7 0.275060648809011 0.8924726372646012 8 0.20468703942332964 0.7590121676967323 9 0.11779217635383005 0.49139118570678236 >10 1.379306610838422 12.900459234182582 >50 0.1890885603878607 6.137520333758338 >100 0.12009570543244975 10.335860305297384 >500 0.012081265163570042 4.002462564626106 >1k 0.009823631011558639 7.703563605342404 >5k 2.6034094256088386E-5 0.0678545481849783 >10k+ 2.5975338096942632E-5 3.8540919662850697 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 320105 3.8639812919111995 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0028366804754662747 0.0 0.0 2 0.003078100090399575 0.0 0.0 3 0.0034160875513061946 0.0 0.0 4 0.00369372010847949 0.0 0.0 5 0.00417655933834609 0.0 0.0 6 0.004550759741492705 0.0 1.2070980746665E-5 7 0.00528708956703927 0.0 1.2070980746665E-5 8 0.00593892252735918 0.0 1.2070980746665E-5 9 0.006747678237385734 0.0 1.2070980746665E-5 10-11 0.010399149913251898 0.0 1.2070980746665E-5 12-13 0.013827308445304757 0.0 1.81064711199975E-5 14-15 0.018106471119997497 0.0 2.414196149333E-5 16-17 0.02289261498605017 0.0 2.414196149333E-5 18-19 0.02882550202303602 0.0 2.414196149333E-5 20-21 0.03571803202938173 0.0 2.414196149333E-5 22-23 0.04442724463810053 0.0 2.414196149333E-5 24-25 0.0540598872739392 0.0 3.6212942239995E-5 26-27 0.06472459876361773 0.0 3.6212942239995E-5 28-29 0.0766809051931894 0.0 3.6212942239995E-5 30-31 0.11195231093494454 0.0 4.22484326133275E-5 32-33 0.12777133120344902 0.0 4.828392298666E-5 34-35 0.14212976280160705 0.0 4.828392298666E-5 36-37 0.1576470085514449 0.0 4.828392298666E-5 38-39 0.17479987219245585 0.0 6.0354903733325E-5 40-41 0.19261663977453342 0.0 6.0354903733325E-5 42-43 0.21144133424895745 0.0 7.242588447999E-5 44-45 0.22995821871434158 0.0 7.242588447999E-5 46-47 0.24945285262020556 0.0 7.242588447999E-5 48-49 0.27075209814769596 0.0 7.84613748533225E-5 50-51 0.29213584054041297 0.0 8.4496865226655E-5 52-53 0.3142015933453166 0.0 8.4496865226655E-5 54-55 0.33715456323510007 0.0 8.4496865226655E-5 56-57 0.36170090258344334 0.0 8.4496865226655E-5 58-59 0.3876776531502665 0.0 8.4496865226655E-5 60-61 0.414698543551676 0.0 8.4496865226655E-5 62-63 0.44131505609807237 0.0 8.4496865226655E-5 64-65 0.47020091302484174 6.0354903733325E-6 9.656784597332E-5 66-67 0.5008551686309974 1.2070980746665E-5 1.146743170933175E-4 68-69 0.5337606621464062 1.2070980746665E-5 1.267452978399825E-4 70-71 0.5690863873015214 1.2070980746665E-5 1.32780788213315E-4 72-73 0.6062046530975163 1.2070980746665E-5 1.32780788213315E-4 74-75 0.646859716252284 1.2070980746665E-5 1.32780788213315E-4 76-77 0.6907437667567846 1.2070980746665E-5 1.4485176895998E-4 78-79 0.7376757398998182 1.81064711199975E-5 1.4485176895998E-4 80-81 0.7896895959371977 2.414196149333E-5 1.4485176895998E-4 82-83 0.8463688860331631 2.414196149333E-5 1.4485176895998E-4 84-85 0.909373370040381 2.414196149333E-5 1.4485176895998E-4 86-87 0.9765000939725852 2.414196149333E-5 1.4485176895998E-4 88-89 1.0505253834015083 2.414196149333E-5 1.4485176895998E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 48185 0.0 72.48699 3 CGGAAGA 48210 0.0 72.34825 4 GATCGGA 49475 0.0 70.69919 1 GAAGAGC 49335 0.0 70.59836 6 ATCGGAA 50955 0.0 68.5558 2 GGAAGAG 55955 0.0 62.44964 5 CGGAGCG 120 0.0 47.576748 1 TCGACGC 70 7.927156E-8 47.501507 2 AGAGCAC 75535 0.0 46.154694 8 GAGCACA 75930 0.0 45.908337 9 AAGAGCA 76095 0.0 45.790066 7 GCCGTCT 39555 0.0 43.975277 50-51 ATGCCGT 39680 0.0 43.710003 48-49 CGTCTTC 39855 0.0 43.65009 52-53 TGCCGTC 39595 0.0 43.636944 50-51 CCGTCTT 39750 0.0 43.4547 52-53 TATGCCG 39655 0.0 43.44409 48-49 TCGTATG 40200 0.0 43.33825 44-45 TCTCGTA 39640 0.0 43.255527 42-43 CGTATGC 39935 0.0 43.20387 46-47 >>END_MODULE