FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_30484-1.34100000035806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_30484-1.34100000035806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8284331
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT3201053.8639812919111995TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG481850.072.486993
CGGAAGA482100.072.348254
GATCGGA494750.070.699191
GAAGAGC493350.070.598366
ATCGGAA509550.068.55582
GGAAGAG559550.062.449645
CGGAGCG1200.047.5767481
TCGACGC707.927156E-847.5015072
AGAGCAC755350.046.1546948
GAGCACA759300.045.9083379
AAGAGCA760950.045.7900667
GCCGTCT395550.043.97527750-51
ATGCCGT396800.043.71000348-49
CGTCTTC398550.043.6500952-53
TGCCGTC395950.043.63694450-51
CCGTCTT397500.043.454752-53
TATGCCG396550.043.4440948-49
TCGTATG402000.043.3382544-45
TCTCGTA396400.043.25552742-43
CGTATGC399350.043.2038746-47
Produced by FastQC (version 0.11.2)