##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_30423-1.3410000003574b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2596973 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67637668932253 33.0 33.0 33.0 33.0 33.0 2 32.69602379385538 33.0 33.0 33.0 33.0 33.0 3 32.73503690642914 33.0 33.0 33.0 33.0 33.0 4 36.66083628901802 37.0 37.0 37.0 37.0 37.0 5 36.66524488317745 37.0 37.0 37.0 37.0 37.0 6 36.558704691962525 37.0 37.0 37.0 37.0 37.0 7 36.43821672385504 37.0 37.0 37.0 37.0 37.0 8 36.380494906955136 37.0 37.0 37.0 37.0 37.0 9 36.41519492116399 37.0 37.0 37.0 37.0 37.0 10-11 36.333453216494746 37.0 37.0 37.0 37.0 37.0 12-13 36.332301491005104 37.0 37.0 37.0 37.0 37.0 14-15 38.142601405559475 40.0 37.0 40.0 35.0 40.0 16-17 38.13726134233971 40.0 37.0 40.0 37.0 40.0 18-19 38.14774816680805 40.0 37.0 40.0 37.0 40.0 20-21 38.132296523683536 40.0 37.0 40.0 37.0 40.0 22-23 37.9013753704794 40.0 37.0 40.0 33.0 40.0 24-25 37.98752143360751 40.0 37.0 40.0 35.0 40.0 26-27 37.89861311611634 40.0 37.0 40.0 33.0 40.0 28-29 37.68240851945707 40.0 37.0 40.0 33.0 40.0 30-31 37.677612936291595 40.0 37.0 40.0 33.0 40.0 32-33 37.55412050876154 40.0 37.0 40.0 33.0 40.0 34-35 37.5377720523086 40.0 37.0 40.0 33.0 40.0 36-37 37.41350680195751 40.0 37.0 40.0 33.0 40.0 38-39 37.072881581749215 38.5 37.0 40.0 33.0 40.0 40-41 36.94860920771991 37.0 37.0 40.0 33.0 40.0 42-43 36.72391857751313 37.0 37.0 40.0 33.0 40.0 44-45 36.48684872734526 37.0 37.0 40.0 33.0 40.0 46-47 36.59416251150859 37.0 37.0 40.0 33.0 40.0 48-49 36.14394431517 37.0 37.0 40.0 33.0 40.0 50-51 36.109209260165585 37.0 37.0 40.0 33.0 40.0 52-53 36.10209617119624 37.0 37.0 40.0 33.0 40.0 54-55 35.60762106498605 37.0 35.0 40.0 30.0 40.0 56-57 35.44948156950419 37.0 33.0 40.0 27.0 40.0 58-59 35.33245647913937 37.0 33.0 40.0 27.0 40.0 60-61 34.9458717899647 37.0 33.0 38.5 27.0 40.0 62-63 34.71914686829628 37.0 33.0 37.0 27.0 40.0 64-65 34.42706643465296 37.0 33.0 37.0 27.0 40.0 66-67 33.61147593756269 37.0 33.0 37.0 27.0 40.0 68-69 33.60483532173804 37.0 33.0 37.0 27.0 37.0 70-71 33.23534977067532 37.0 33.0 37.0 27.0 37.0 72-73 32.30638420191507 35.0 33.0 37.0 22.0 37.0 74-75 31.359866275082567 33.0 30.0 37.0 15.0 37.0 76-77 28.737370777439736 33.0 27.0 33.0 15.0 35.0 78-79 30.89154546466213 33.0 30.0 37.0 15.0 37.0 80-81 31.309279880845892 33.0 33.0 37.0 15.0 37.0 82-83 31.134604595427064 33.0 33.0 37.0 15.0 37.0 84-85 30.96672876460402 33.0 33.0 37.0 15.0 37.0 86-87 30.819523730127344 33.0 33.0 37.0 15.0 37.0 88-89 30.731193393231273 33.0 33.0 37.0 15.0 37.0 90-91 30.610025980247002 33.0 33.0 37.0 15.0 37.0 92-93 30.442142448150214 33.0 30.0 37.0 15.0 37.0 94-95 30.20889551027292 33.0 27.0 37.0 15.0 37.0 96-97 30.048614290560586 33.0 27.0 37.0 15.0 37.0 98-99 29.899896148323453 33.0 27.0 37.0 15.0 37.0 100-101 28.33876671032005 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16146311168041905 2306 2 0.13484032049610306 2306 3 0.09990610159706392 2306 4 0.15840956177414256 2306 5 0.09882303899523492 2306 6 0.07643768839029974 2306 7 0.09946043473261312 2306 8 0.2079442078400504 2306 9 0.184630911499859 2306 10-11 0.1948889730032235 2306 12-13 0.22509269221050943 2306 14-15 0.39334367731524367 2306 16-17 0.3575431142265941 2306 18-19 0.4301150739164612 2306 20-21 0.30864095274154124 2306 22-23 0.1752974193773298 2306 24-25 0.3161179388899171 2306 26-27 0.49297324984101465 2306 28-29 0.4383503988398516 2306 30-31 0.5321964967218733 2306 32-33 0.5192706197440558 2306 34-35 0.5671009679095889 2306 36-37 0.511978649839854 2306 38-39 0.33986585756614573 2306 40-41 0.4314479536568214 2306 42-43 0.5078096874065281 2306 44-45 0.4511877007392471 2306 46-47 0.2303954106665529 2306 48-49 0.06564793669521407 2306 50-51 0.02965447797618026 2306 52-53 0.25424919688425973 2306 54-55 -0.04230160903867386 2306 56-57 0.0515556916880584 2306 58-59 0.09069477840539264 2306 60-61 -0.06920509095083816 2306 62-63 -0.14545478331594097 2306 64-65 -0.029315960782177797 2306 66-67 -0.06681289612596686 2306 68-69 0.3286991405123203 2306 70-71 0.5058125767809543 2306 72-73 0.1109229994114358 2306 74-75 -0.07075602252423963 2306 76-77 -0.5001493615625598 2306 78-79 -0.08109291993717349 2306 80-81 -0.03936580441954263 2306 82-83 -7.240842372802092E-4 2306 84-85 -0.057245903702497714 2306 86-87 -0.1197219513665253 2306 88-89 -0.18566294732880095 2306 90-91 -0.22936409532043456 2306 92-93 -0.23140539754547262 2306 94-95 -0.23391286621705376 2306 96-97 -0.05881035404569346 2306 98-99 -0.11197377983896217 2306 100-101 -0.16439404274240843 2307 1 0.20150066234671016 2307 2 0.14139169170545074 2307 3 0.1452329415119351 2307 4 0.18394469265922453 2307 5 0.14802186773979287 2307 6 0.15992962923105125 2307 7 0.16544251343989913 2307 8 0.21048701153276284 2307 9 0.2532164578823881 2307 10-11 0.24075568847219841 2307 12-13 0.24877011571966534 2307 14-15 0.34746510017370014 2307 16-17 0.35994764470770235 2307 18-19 0.28682691661034454 2307 20-21 0.30576229426282 2307 22-23 0.2738909639162159 2307 24-25 0.43918482516697566 2307 26-27 0.3660808047097177 2307 28-29 0.4249487422725551 2307 30-31 0.5089893038036237 2307 32-33 0.4858654385654759 2307 34-35 0.5117918527225882 2307 36-37 0.43578689065213894 2307 38-39 0.3683991109179203 2307 40-41 0.41272345625283435 2307 42-43 0.3576432850857074 2307 44-45 0.4304472273568365 2307 46-47 0.2205019284861791 2307 48-49 -0.11807225661615206 2307 50-51 -0.11733587678949675 2307 52-53 0.10115346979763018 2307 54-55 -0.10495852202144107 2307 56-57 -0.046636081055851264 2307 58-59 0.14326706682705037 2307 60-61 -0.176524081827516 2307 62-63 -0.1661240866629683 2307 64-65 -0.1591545064733353 2307 66-67 -0.03413415766568306 2307 68-69 0.09277352336643929 2307 70-71 0.19253682339528666 2307 72-73 0.20282842460157013 2307 74-75 0.13963870004818446 2307 76-77 -0.19516610346612495 2307 78-79 0.19672964977556973 2307 80-81 0.1988187835398456 2307 82-83 0.31729294912452133 2307 84-85 0.378256421185327 2307 86-87 0.2895472679320754 2307 88-89 0.2942864202745987 2307 90-91 0.14601615601146278 2307 92-93 0.22007883659716754 2307 94-95 0.17284472864243838 2307 96-97 0.326155484337324 2307 98-99 0.20989728039122468 2307 100-101 0.132722215204911 2201 1 0.1148816847293972 2201 2 0.11428704848145799 2201 3 0.10553119284511325 2201 4 0.0708844334653449 2201 5 0.09311231496302241 2201 6 0.0857506046217793 2201 7 0.15816521781494686 2201 8 0.10260324897748063 2201 9 0.18100906587471144 2201 10-11 0.20392112600794832 2201 12-13 0.11046198008266117 2201 14-15 0.3173930130294522 2201 16-17 0.3018719778093626 2201 18-19 0.2118504433447086 2201 20-21 0.24673125742579316 2201 22-23 0.40977261675387666 2201 24-25 0.3710590909175835 2201 26-27 0.35192201616739993 2201 28-29 0.4124106843199016 2201 30-31 0.4047552604652296 2201 32-33 0.4172356478765309 2201 34-35 0.4032384239701443 2201 36-37 0.3012454928258208 2201 38-39 0.4410129157290612 2201 40-41 0.27302309151156834 2201 42-43 0.29847262553754916 2201 44-45 0.3229343568492098 2201 46-47 0.27429203926173074 2201 48-49 0.04514730778559084 2201 50-51 0.14679661716736092 2201 52-53 0.19718898440265065 2201 54-55 0.2384932035799494 2201 56-57 0.12420107088687615 2201 58-59 0.21914816609449161 2201 60-61 0.006728727541350565 2201 62-63 0.047949667342727764 2201 64-65 0.027408037735391133 2201 66-67 0.13162844682069874 2201 68-69 0.19957613252740458 2201 70-71 0.25860266134683485 2201 72-73 0.17266915014760542 2201 74-75 0.20381286554107803 2201 76-77 0.08662051366626144 2201 78-79 0.40702728603072913 2201 80-81 0.5625294877426725 2201 82-83 0.5300819589104115 2201 84-85 0.634954774621459 2201 86-87 0.49980246380367177 2201 88-89 0.4690614554878145 2201 90-91 0.4857651119165176 2201 92-93 0.40964373040394264 2201 94-95 0.36168490676903176 2201 96-97 0.45972844181698136 2201 98-99 0.5059530925709979 2201 100-101 0.45797239819061986 1101 1 0.14093782571509195 1101 2 0.137931226636411 1101 3 0.06431692260850497 1101 4 0.10481337680894143 1101 5 0.1162626350503686 1101 6 0.10223252594529697 1101 7 0.19384869844666497 1101 8 0.1304849292099064 1101 9 0.11088384997616885 1101 10-11 0.05985236665935645 1101 12-13 0.003126741442450509 1101 14-15 0.14481407462969287 1101 16-17 0.09631604825171536 1101 18-19 0.09378733470014566 1101 20-21 0.08675411479705986 1101 22-23 0.07654490979106043 1101 24-25 0.07572565520118246 1101 26-27 0.02392925731529516 1101 28-29 0.021519131133651115 1101 30-31 0.20701033526447787 1101 32-33 0.2493480981488645 1101 34-35 0.08248392034220586 1101 36-37 0.09734022732057923 1101 38-39 0.04764076448542909 1101 40-41 0.019646290954490553 1101 42-43 0.04955054045721141 1101 44-45 0.044612990413888554 1101 46-47 -0.12696474799957258 1101 48-49 -0.1324659324492714 1101 50-51 -0.08102841928031523 1101 52-53 -0.06539414048295811 1101 54-55 -0.17549949426306455 1101 56-57 -0.1374361501280248 1101 58-59 -0.19701508792001476 1101 60-61 -0.11005976446119803 1101 62-63 -0.14741695521506415 1101 64-65 -0.20865690749317167 1101 66-67 -0.22181404369659674 1101 68-69 -0.10769588807261954 1101 70-71 -0.19695011256820294 1101 72-73 -0.13320439483818802 1101 74-75 0.01313631680427818 1101 76-77 0.07330790939856868 1101 78-79 0.15915997449096508 1101 80-81 0.3035906041248744 1101 82-83 0.35680299379288627 1101 84-85 0.4892979381321325 1101 86-87 0.32838322406108844 1101 88-89 0.3463942642832194 1101 90-91 0.2108179163361008 1101 92-93 0.301753887575984 1101 94-95 0.43848614756770843 1101 96-97 0.3097389080316155 1101 98-99 0.14423440338286042 1101 100-101 0.1955391663287287 2304 1 0.024020905748947996 2304 2 0.05370887524883727 2304 3 -0.028242906015051972 2304 4 0.058419230743780304 2304 5 0.05665094891559619 2304 6 0.09896858514328244 2304 7 0.09067046233312936 2304 8 0.13035731896288638 2304 9 0.07479361388897843 2304 10-11 0.021090342480924562 2304 12-13 -0.013424307596110907 2304 14-15 0.20613594389752876 2304 16-17 0.08254443272245027 2304 18-19 0.16970884534202213 2304 20-21 0.12365158860814063 2304 22-23 0.1419317828954263 2304 24-25 0.15928175760552676 2304 26-27 0.11652839812784777 2304 28-29 0.07224585090813207 2304 30-31 0.09588753798200145 2304 32-33 0.2501787996923639 2304 34-35 0.18814438158326396 2304 36-37 0.23915328901149024 2304 38-39 0.27137219513625155 2304 40-41 0.19363195414906187 2304 42-43 0.23515902727960025 2304 44-45 0.22677970538034486 2304 46-47 0.1463138309327192 2304 48-49 0.2225346603209033 2304 50-51 0.21265071586799422 2304 52-53 0.0022868355460801126 2304 54-55 -0.03732208967436179 2304 56-57 0.10606560677693011 2304 58-59 0.0425400133613536 2304 60-61 0.025635414999975126 2304 62-63 0.003993426166680081 2304 64-65 -0.023326693338468374 2304 66-67 -0.018424098066795125 2304 68-69 0.12497834519561479 2304 70-71 0.20160591573986153 2304 72-73 -0.16484700217075243 2304 74-75 -0.1789078429275257 2304 76-77 -0.5604298935316905 2304 78-79 -0.0030028051401345124 2304 80-81 -0.022257221890487955 2304 82-83 0.3556598529671575 2304 84-85 0.3865784814389315 2304 86-87 0.34370077598611104 2304 88-89 0.1516544409945979 2304 90-91 0.20673829906804642 2304 92-93 -0.14052367541408017 2304 94-95 -0.046811711214679974 2304 96-97 -0.032130590320154084 2304 98-99 0.03637678184027493 2304 100-101 -0.14149946088139842 2202 1 0.012831270884404944 2202 2 0.07091122858193444 2202 3 0.056656440810094466 2202 4 0.08573003013938774 2202 5 -0.005409585759778679 2202 6 -0.006644376035048083 2202 7 0.08008760192694808 2202 8 0.05120894476150539 2202 9 0.07465679219738064 2202 10-11 0.06260531553299842 2202 12-13 0.06635984131347783 2202 14-15 -0.03838970124649421 2202 16-17 -0.006228306447816578 2202 18-19 0.023725305586388856 2202 20-21 0.027094141343980027 2202 22-23 -0.08559822864518907 2202 24-25 -0.06819471501229657 2202 26-27 -0.019766083793847145 2202 28-29 -0.005732846549356907 2202 30-31 -0.05248740699187948 2202 32-33 -0.06698057378326183 2202 34-35 -0.024002856403193107 2202 36-37 -0.03919854499405773 2202 38-39 0.07274502234830038 2202 40-41 0.026597207388171284 2202 42-43 -0.17374152119691644 2202 44-45 0.005656518267763033 2202 46-47 -0.10110365963848977 2202 48-49 -0.20350503581596513 2202 50-51 -0.05548485063329167 2202 52-53 0.009692309603494209 2202 54-55 -0.15765602383338262 2202 56-57 -0.009207411985485692 2202 58-59 0.03520518093818481 2202 60-61 -0.17478828608627595 2202 62-63 -0.14143762164952278 2202 64-65 -0.163330232856147 2202 66-67 -0.1136839700492871 2202 68-69 0.012229091766151612 2202 70-71 -0.08212211842986505 2202 72-73 -0.28958079474907095 2202 74-75 -0.1950587513045825 2202 76-77 -0.3747316270030332 2202 78-79 -0.1723129561198995 2202 80-81 0.00841611929521946 2202 82-83 0.1628676791679169 2202 84-85 0.2395152400735725 2202 86-87 -0.05181652898349043 2202 88-89 -0.05847690782933057 2202 90-91 -0.15648983028199126 2202 92-93 -0.157595155824783 2202 94-95 -0.02712797752917595 2202 96-97 0.02396704682871942 2202 98-99 -0.10526489999575261 2202 100-101 -0.13893107977944652 1102 1 0.07622350927663746 1102 2 0.07598413365484191 1102 3 0.0556137897674418 1102 4 0.038199282608637475 1102 5 0.03541813006793859 1102 6 0.043418274027601456 1102 7 0.06864259048193588 1102 8 0.036134079686640064 1102 9 -0.06342972088913257 1102 10-11 0.007540372968058762 1102 12-13 0.023784136237772202 1102 14-15 -0.05525403061081846 1102 16-17 -0.0402421279616334 1102 18-19 0.025814720175809214 1102 20-21 -0.10798744373759916 1102 22-23 -0.06882034936730719 1102 24-25 -0.028371412688997566 1102 26-27 -0.05540259443035467 1102 28-29 -0.04720622552273568 1102 30-31 -0.08015003465451542 1102 32-33 -0.07412311842580976 1102 34-35 -0.1007263456266756 1102 36-37 -0.09397456227007694 1102 38-39 -0.17133192172864398 1102 40-41 -0.1238012680103111 1102 42-43 -0.2746985096539092 1102 44-45 -0.18216670705546534 1102 46-47 -0.25929323032805485 1102 48-49 -0.37636872117965225 1102 50-51 -0.36369025883870165 1102 52-53 -0.27506150015031494 1102 54-55 -0.29932199049935093 1102 56-57 -0.29334338362146184 1102 58-59 -0.2828025995695995 1102 60-61 -0.27338688468486794 1102 62-63 -0.27976335997527 1102 64-65 -0.3484303679562899 1102 66-67 -0.2592396656049729 1102 68-69 -0.09250125046418844 1102 70-71 -0.23973812604587152 1102 72-73 -0.4329893256576014 1102 74-75 -0.4383369070827392 1102 76-77 -0.36279145256286327 1102 78-79 -0.011497420304362294 1102 80-81 0.15466236554146562 1102 82-83 0.12742454372478207 1102 84-85 0.07594572650405951 1102 86-87 -0.03692970159666231 1102 88-89 -0.04004203939446427 1102 90-91 0.12013399634183486 1102 92-93 -0.09792350865313537 1102 94-95 -0.19197481737602118 1102 96-97 -0.002333641971969058 1102 98-99 -0.13375326598411874 1102 100-101 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0.10977499435302818 1213 78-79 -0.565007157828969 1213 80-81 -0.511513661786978 1213 82-83 -0.523620588175369 1213 84-85 -0.4274844613127442 1213 86-87 -0.5160480413097979 1213 88-89 -0.6751575217218395 1213 90-91 -0.5097904949209457 1213 92-93 -0.42335473873120577 1213 94-95 -0.2815023550448217 1213 96-97 -0.3053990383459144 1213 98-99 -0.3393345529780447 1213 100-101 -0.0506349703402158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 10.0 10 10.0 11 11.0 12 31.0 13 84.0 14 234.0 15 720.0 16 1504.0 17 2697.0 18 3627.0 19 4632.0 20 6574.0 21 9072.0 22 12366.0 23 16565.0 24 22019.0 25 29104.0 26 39015.0 27 56048.0 28 87516.0 29 106599.0 30 72815.0 31 61440.0 32 80462.0 33 115407.0 34 179748.0 35 313087.0 36 570466.0 37 691578.0 38 113504.0 39 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72479390637806 21.5482427806971 21.354442462517476 12.372520850407366 2 31.307179550961834 27.470982563161034 29.618097685266655 11.60374020061048 3 38.009513093210906 14.788231243949381 32.44352178530692 14.758733877532787 4 31.299847005242178 14.1564628443778 29.87672234628417 24.666967804095854 5 39.08538902791827 13.011841093457651 32.47623290654158 15.426536972082497 6 34.15310825333956 13.730369934535322 36.55729189329269 15.559229918832425 7 33.56684878895545 23.97075364279875 25.310698262939198 17.1516993053066 8 29.87686048333964 33.73747050893483 19.87321393021799 16.512455077507546 9 38.63259263765931 23.65954517047347 20.326472396902084 17.381389794965138 10-11 30.284431143488977 28.946373335417814 25.00266656603669 15.766528955056522 12-13 25.243514369812225 27.353048088312082 24.72956966805957 22.67386787381612 14-15 24.02423898900759 28.143804344519562 24.593093574711787 23.23886309176106 16-17 27.904319356481444 24.817975546525812 25.306274902795465 21.971430194197282 18-19 22.02450699333416 24.89987381462957 30.09497980918554 22.980639382850725 20-21 26.17899762531224 25.58869499220824 30.357188927262623 17.875118455216903 22-23 21.94821695056488 33.942139097128404 25.646354337238076 18.46328961506864 24-25 22.599251204576408 22.962597580026888 31.1525812186582 23.28556999673851 26-27 20.487794828825713 23.049565782932667 27.37720030204396 29.085439086197663 28-29 24.7097678720572 29.51957914079199 25.254113154045115 20.516539833105696 30-31 18.44383826863044 25.879283304062074 30.57297861779849 25.103899809508995 32-33 19.03974357838915 28.9207088406387 26.541265542614422 25.498282038357733 34-35 19.903518442432784 24.02529791414851 30.832030213637186 25.23915342978152 36-37 23.717901572330554 26.44453754428714 26.28508267124841 23.552478212133895 38-39 23.796676335690325 30.93163411120648 24.158966296271053 21.11272325683214 40-41 24.801008712836058 30.03685444554102 23.960857506027207 21.20127933559571 42-43 21.085009355122292 30.434509715734432 29.10490405560628 19.375576873537 44-45 18.991935980728293 26.826225814103218 28.982913921157287 25.1989242840112 46-47 24.326503076012838 26.21770273160876 25.26108035601 24.1947138363684 48-49 19.867315565189216 29.867975964544062 30.477911566942833 19.78679690332389 50-51 24.03284532051989 25.318829454664403 28.661429390242937 21.986895834572763 52-53 18.95690697513227 26.230890971682058 24.61215892550463 30.20004312768104 54-55 24.387567183016017 26.09330100012804 29.963832120196347 19.5552996966596 56-57 20.104691154931704 25.500018965091332 28.636325216081705 25.758964663895263 58-59 18.932754322699846 24.720915988968915 29.625100626972706 26.72122906135853 60-61 22.889907223555095 24.747573251072463 31.33694001193327 21.025579513439176 62-63 18.797390865874185 24.73921847272198 31.06798023145197 25.395410429951866 64-65 24.643378772656927 26.352003105189446 29.471574847811443 19.53304327434218 66-67 20.13102327661781 35.06959841388822 24.570811546021908 20.228566763472063 68-69 19.57989715484325 33.3162354752444 26.52368775383243 20.580179616079917 70-71 19.203534234041342 36.118592621916754 25.265637491470176 19.412235652571734 72-73 21.080036459851613 35.42044932492667 24.525103501231694 18.974410713990025 74-75 22.404264368840796 33.28116603161577 24.751138287571095 19.563431311972337 76-77 21.608344685172973 31.52704465484792 25.112900663781772 21.751709996197334 78-79 21.584920453230495 32.425361546031304 25.141762087683684 20.847955913054513 80-81 21.118431933392134 32.60414554798444 25.09416405252278 21.183258466100654 82-83 20.43228789825693 31.674318523912266 25.992800079169093 21.90059349866171 84-85 21.02419653730079 30.975880475748106 25.86941474896153 22.130508237989574 86-87 19.25237153655545 31.538172737582045 27.110181386205717 22.099274339656787 88-89 18.992074104666486 31.70587255606996 27.20926269823162 22.092790641031936 90-91 20.40827250411513 30.74907593692977 25.914950962128863 22.927700596826234 92-93 20.522543605471164 30.91276364156589 25.038793258647207 23.52589949431575 94-95 20.797314532700348 31.959587059726 24.89550568547654 22.347592722097108 96-97 20.22553278341305 33.1745765844867 23.82874296232969 22.77114766977056 98-99 20.49522740812082 30.91446534147513 25.426976282796527 23.163330967607525 100-101 21.711732249746614 31.56742816565568 24.847928687835218 21.872910896762487 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 235.0 1 166.0 2 116.0 3 215.5 4 242.0 5 173.5 6 170.5 7 182.0 8 196.5 9 211.0 10 200.0 11 191.5 12 204.0 13 209.0 14 208.0 15 224.0 16 230.5 17 235.5 18 252.5 19 270.0 20 297.5 21 341.5 22 370.0 23 387.0 24 486.0 25 609.0 26 760.0 27 975.0 28 1288.0 29 1734.5 30 2316.0 31 3377.5 32 5525.5 33 9661.5 34 16420.5 35 27899.0 36 45349.5 37 68497.0 38 95691.5 39 117229.5 40 131774.5 41 140685.5 42 173206.5 43 212128.5 44 204457.5 45 179352.5 46 171974.0 47 159949.5 48 132032.5 49 109620.5 50 92888.5 51 83956.5 52 81694.5 53 81972.0 54 68673.0 55 42218.0 56 27122.0 57 21720.0 58 18916.5 59 15296.0 60 11528.5 61 8926.5 62 6512.0 63 4550.0 64 3336.0 65 2382.5 66 1761.5 67 1341.5 68 1040.5 69 826.5 70 630.0 71 493.0 72 307.5 73 160.0 74 106.0 75 65.5 76 51.0 77 31.0 78 18.0 79 11.0 80 5.5 81 3.5 82 5.0 83 4.0 84 2.0 85 1.5 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1577990991820092 2 0.0 3 0.005044334307672818 4 0.005852968051650903 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.85063687608612E-5 14-15 0.0 16-17 1.92531843804306E-5 18-19 0.0 20-21 0.0 22-23 1.347722906630142E-4 24-25 7.70127375217224E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.701273752172239E-4 40-41 0.0 42-43 0.0 44-45 1.9253184380430599E-4 46-47 9.048996658802382E-4 48-49 0.0022911289412712414 50-51 4.8132960951076506E-4 52-53 0.0012707101691084197 54-55 0.002137103466227797 56-57 0.0037158645854231064 58-59 0.0049865747545315255 60-61 0.0012129506159671277 62-63 7.508741908367935E-4 64-65 0.0011551910628258362 66-67 0.007027412298857169 68-69 0.006026246711074778 70-71 0.007739780120933102 72-73 0.005544917101564013 74-75 0.00768202056779181 76-77 0.004254953748075162 78-79 0.004023915535509996 80-81 0.0016365206723366011 82-83 0.0 84-85 4.0431687198904265E-4 86-87 0.0010781783253041137 88-89 0.004062421904270857 90-91 0.005910727604792194 92-93 0.007489488723987504 94-95 0.006969652745715878 96-97 0.00897198392128066 98-99 0.0063920572143029595 100-101 0.006353550845542099 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2596973.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.674708654246796 #Duplication Level Percentage of deduplicated Percentage of total 1 82.87021397515902 26.24889883778253 2 7.348077127754251 4.65496404381101 3 2.7433771015124866 2.6068701125742018 4 1.3673869524891735 1.732463333508519 5 0.986042392578911 1.5616302752836726 6 0.6975162003522307 1.3256173456664488 7 0.4701637963210404 1.042461088777051 8 0.3940284765889869 0.998458975794629 9 0.33799405964800777 0.9635277029595083 >10 2.1968420737743353 14.070338995820741 >50 0.2907798619106525 6.43683054307595 >100 0.2658881410269326 16.72480008192836 >500 0.02145124365791131 4.523246086654742 >1k 0.0092553787858314 4.745124700221112 >5k 3.687069151391539E-4 0.7382096229310728 >10k+ 6.145115250984735E-4 11.626558253210236 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 222471 8.566550364597553 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 27956 1.0764840450786357 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 25925 0.9982776101253267 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12047 0.46388622446209493 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10262 0.3951523562239576 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 8773 0.33781637313903534 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5101 0.19642098704915298 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5089 0.19595891062402265 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 4929 0.18979789162228486 TruSeq Adapter, Index 6 (97% over 36bp) TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 3700 0.14247356441518647 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3206 0.123451418247321 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2927 0.11270814136304073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004351219669977316 0.0 0.0 2 0.004659270620064206 0.0 0.0 3 0.005159853413955401 0.0 0.0 4 0.005352385257759707 0.0 0.0 5 0.006122512632976931 0.0 0.0 6 0.006815627270672433 0.0 0.0 7 0.007970818333498268 0.0 0.0 8 0.008856464814998077 0.0 0.0 9 0.010011655877823912 0.0 0.0 10-11 0.016538485382789885 0.0 0.0 12-13 0.022564732093864663 0.0 0.0 14-15 0.02907230841445021 0.0 0.0 16-17 0.03704312674794848 0.0 0.0 18-19 0.04570705971914225 0.0 0.0 20-21 0.05132898955822798 0.0 0.0 22-23 0.05671988118474855 0.0 0.0 24-25 0.062322557839453854 0.0 0.0 26-27 0.06925370421640886 0.0 0.0 28-29 0.07643514199030949 0.0 0.0 30-31 0.12943915858963495 0.0 0.0 32-33 0.1390464975954698 0.0 0.0 34-35 0.1483650388355982 0.0 0.0 36-37 0.15766432689134618 0.0 0.0 38-39 0.16761822321602882 0.0 0.0 40-41 0.17847701920659167 0.0 0.0 42-43 0.19104934860701286 0.0 0.0 44-45 0.20337138661048845 0.0 0.0 46-47 0.21702189433621372 0.0 0.0 48-49 0.23225116318113434 0.0 0.0 50-51 0.24826981258565262 0.0 0.0 52-53 0.2655784253436597 0.0 0.0 54-55 0.2844080396677208 0.0 0.0 56-57 0.30531699790486844 0.0 0.0 58-59 0.33005733983372176 0.0 0.0 60-61 0.3570310511507051 0.0 0.0 62-63 0.3873740697342637 0.0 0.0 64-65 0.42004672362785445 0.0 0.0 66-67 0.45518378512214025 0.0 0.0 68-69 0.49343986248605587 0.0 0.0 70-71 0.5366055018669813 0.0 0.0 72-73 0.5840260949959819 0.0 0.0 74-75 0.6347197294696556 0.0 0.0 76-77 0.6905154578041435 0.0 0.0 78-79 0.7537814216782385 0.0 0.0 80-81 0.8249219379639295 0.0 0.0 82-83 0.9053039827522273 0.0 0.0 84-85 1.0003377008540328 0.0 0.0 86-87 1.1003579937103698 0.0 0.0 88-89 1.2102359169694872 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 29685 0.0 81.62378 3 CGGAAGA 29575 0.0 81.59009 4 GATCGGA 29765 0.0 81.41339 1 GAAGAGC 29600 0.0 81.3881 6 ATCGGAA 30020 0.0 80.68128 2 GGAAGAG 31285 0.0 77.201935 5 CGAGTCG 20 0.0020621088 71.35658 1 GAGCACA 37800 0.0 63.694782 9 AAGAGCA 37875 0.0 63.606277 7 AGAGCAC 37930 0.0 63.47648 8 GCCGTCT 25475 0.0 45.886776 50-51 ATGCCGT 25345 0.0 45.84102 48-49 TCGTATG 25860 0.0 45.80057 44-45 TTGTGTT 24605 0.0 45.64734 1 TCTCGTA 25365 0.0 45.636333 42-43 AATCTCG 25475 0.0 45.493904 40-41 CTGCTTG 25720 0.0 45.394264 58-59 GTATGCC 25930 0.0 45.319725 46-47 TGCCGTC 25490 0.0 45.2821 50-51 TATGCCG 25595 0.0 45.21696 48-49 >>END_MODULE