FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_30423-1.3410000003574b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_30423-1.3410000003574b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2596973
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2224718.566550364597553TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA279561.0764840450786357No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC259250.9982776101253267No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC120470.46388622446209493No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT102620.3951523562239576No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT87730.33781637313903534No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC51010.19642098704915298No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT50890.19595891062402265No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT49290.18979789162228486TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT37000.14247356441518647No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32060.123451418247321No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG29270.11270814136304073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG296850.081.623783
CGGAAGA295750.081.590094
GATCGGA297650.081.413391
GAAGAGC296000.081.38816
ATCGGAA300200.080.681282
GGAAGAG312850.077.2019355
CGAGTCG200.002062108871.356581
GAGCACA378000.063.6947829
AAGAGCA378750.063.6062777
AGAGCAC379300.063.476488
GCCGTCT254750.045.88677650-51
ATGCCGT253450.045.8410248-49
TCGTATG258600.045.8005744-45
TTGTGTT246050.045.647341
TCTCGTA253650.045.63633342-43
AATCTCG254750.045.49390440-41
CTGCTTG257200.045.39426458-59
GTATGCC259300.045.31972546-47
TGCCGTC254900.045.282150-51
TATGCCG255950.045.2169648-49
Produced by FastQC (version 0.11.2)