##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_30245-1.34100000035771.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2180830 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.7023862474379 33.0 33.0 33.0 33.0 33.0 2 32.74294878555413 33.0 33.0 33.0 33.0 33.0 3 32.7714338118973 33.0 33.0 33.0 33.0 33.0 4 36.72014187258979 37.0 37.0 37.0 37.0 37.0 5 36.72292888487411 37.0 37.0 37.0 37.0 37.0 6 36.60821476226941 37.0 37.0 37.0 37.0 37.0 7 36.55859833182779 37.0 37.0 37.0 37.0 37.0 8 36.50004493701939 37.0 37.0 37.0 37.0 37.0 9 36.50962064901895 37.0 37.0 37.0 37.0 37.0 10-11 36.46179619686083 37.0 37.0 37.0 37.0 37.0 12-13 36.45365938656383 37.0 37.0 37.0 37.0 37.0 14-15 38.37484283506738 40.0 37.0 40.0 37.0 40.0 16-17 38.357226835654316 40.0 37.0 40.0 37.0 40.0 18-19 38.37999798241954 40.0 37.0 40.0 37.0 40.0 20-21 38.333353814831966 40.0 37.0 40.0 37.0 40.0 22-23 38.23560662683474 40.0 37.0 40.0 37.0 40.0 24-25 38.218334533182315 40.0 37.0 40.0 37.0 40.0 26-27 38.15325197287271 40.0 37.0 40.0 37.0 40.0 28-29 38.0356183196306 40.0 37.0 40.0 37.0 40.0 30-31 38.00443890628797 40.0 37.0 40.0 37.0 40.0 32-33 37.876711848241264 40.0 37.0 40.0 33.0 40.0 34-35 37.827528509787555 40.0 37.0 40.0 33.0 40.0 36-37 37.75229706121064 40.0 37.0 40.0 33.0 40.0 38-39 37.624673404162635 40.0 37.0 40.0 33.0 40.0 40-41 37.56566720010271 40.0 37.0 40.0 33.0 40.0 42-43 37.446596479322096 40.0 37.0 40.0 33.0 40.0 44-45 37.310486145183255 40.0 37.0 40.0 33.0 40.0 46-47 37.280208223474546 40.0 37.0 40.0 33.0 40.0 48-49 37.08857040668002 40.0 37.0 40.0 33.0 40.0 50-51 36.96387682671276 37.0 37.0 40.0 33.0 40.0 52-53 36.844636216486386 37.0 37.0 40.0 33.0 40.0 54-55 36.66020460100054 37.0 37.0 40.0 33.0 40.0 56-57 36.50784701237602 37.0 37.0 40.0 33.0 40.0 58-59 36.322266293108584 37.0 37.0 40.0 33.0 40.0 60-61 36.074989568191924 37.0 37.0 40.0 33.0 40.0 62-63 35.84984891073582 37.0 37.0 40.0 33.0 40.0 64-65 35.615260703493625 37.0 35.0 40.0 27.0 40.0 66-67 35.29125722775274 37.0 33.0 38.5 27.0 40.0 68-69 35.07101447613982 37.0 33.0 37.0 27.0 40.0 70-71 34.736680071348985 37.0 33.0 37.0 27.0 40.0 72-73 34.376696028576276 37.0 33.0 37.0 27.0 40.0 74-75 33.96736701164235 37.0 33.0 37.0 27.0 38.5 76-77 31.273531866307778 33.0 30.0 35.0 24.5 37.0 78-79 33.63216802776924 37.0 33.0 37.0 27.0 37.0 80-81 33.97131550831564 37.0 33.0 37.0 27.0 37.0 82-83 33.89472631979568 37.0 33.0 37.0 27.0 37.0 84-85 33.70476722165414 37.0 33.0 37.0 27.0 37.0 86-87 33.53261350036454 37.0 33.0 37.0 27.0 37.0 88-89 33.37778987816566 37.0 33.0 37.0 27.0 37.0 90-91 33.21681515753177 37.0 33.0 37.0 27.0 37.0 92-93 33.09487626270732 37.0 33.0 37.0 27.0 37.0 94-95 32.92228555183118 37.0 33.0 37.0 27.0 37.0 96-97 32.763998110811016 33.0 33.0 37.0 27.0 37.0 98-99 32.63725439396927 33.0 33.0 37.0 27.0 37.0 100-101 31.08472485246443 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14769376280218438 2306 2 0.16200647281399227 2306 3 0.15097932520358626 2306 4 0.1309424677511899 2306 5 0.1428796480629444 2306 6 0.16572870808440854 2306 7 0.16905401735125025 2306 8 0.21319942295767902 2306 9 0.14045781665125645 2306 10-11 0.2552520393321842 2306 12-13 0.20350226311347797 2306 14-15 0.4373057020874995 2306 16-17 0.315594571351447 2306 18-19 0.3896932734200078 2306 20-21 0.19525459942439483 2306 22-23 0.3765762470764713 2306 24-25 0.36836867246802996 2306 26-27 0.2816674021320509 2306 28-29 0.2917671402047475 2306 30-31 0.3258327657439608 2306 32-33 0.36866927581096576 2306 34-35 0.5176950581519932 2306 36-37 0.4702043161549909 2306 38-39 0.40700933061284417 2306 40-41 0.35914330046746556 2306 42-43 0.5156472540856853 2306 44-45 0.5118665130077673 2306 46-47 0.40312218484147166 2306 48-49 0.3666409492041325 2306 50-51 0.32916471022081595 2306 52-53 0.3349942540954629 2306 54-55 0.2927466005472539 2306 56-57 0.25139411375155873 2306 58-59 0.23282055756551756 2306 60-61 0.1566266877275453 2306 62-63 0.317647380129344 2306 64-65 0.09988959980842083 2306 66-67 0.3077828058793983 2306 68-69 0.4071056908461088 2306 70-71 0.33987897212747953 2306 72-73 0.188429876372588 2306 74-75 0.341174659796458 2306 76-77 -0.0400090357235392 2306 78-79 0.23725936239424783 2306 80-81 0.2968235711742011 2306 82-83 0.31183628263807606 2306 84-85 0.3799317921090619 2306 86-87 0.37340830958901705 2306 88-89 0.34434601567235745 2306 90-91 0.35932216570923003 2306 92-93 0.31322285053789045 2306 94-95 0.3699749722501835 2306 96-97 0.5500774674904676 2306 98-99 0.29965999960295875 2306 100-101 0.17698824350293663 2307 1 0.1328802678843104 2307 2 0.14001852825063565 2307 3 0.09737482835517852 2307 4 0.1301881830863465 2307 5 0.12403838235141507 2307 6 0.1548560772488159 2307 7 0.18862661123782942 2307 8 0.13698005074115116 2307 9 0.2138480747915139 2307 10-11 0.13933242421413183 2307 12-13 0.2143874653601543 2307 14-15 0.42581215286113405 2307 16-17 0.4837483995100911 2307 18-19 0.4147885428526763 2307 20-21 0.38737855572398416 2307 22-23 0.39394611110846256 2307 24-25 0.43476731300656724 2307 26-27 0.4321327083169635 2307 28-29 0.39917345039721397 2307 30-31 0.4866522687031605 2307 32-33 0.41445353509249117 2307 34-35 0.4985356810818118 2307 36-37 0.515234837354761 2307 38-39 0.4580678434258445 2307 40-41 0.42142671691787115 2307 42-43 0.35857239187928514 2307 44-45 0.27615419499260696 2307 46-47 0.39506008425706796 2307 48-49 0.27976266014038487 2307 50-51 0.30191371707935133 2307 52-53 0.3906364127051276 2307 54-55 0.2993892248457044 2307 56-57 0.23009814338073653 2307 58-59 0.4695027872747204 2307 60-61 0.37595353785665253 2307 62-63 0.3147332723825116 2307 64-65 0.22072053728587093 2307 66-67 0.29793802454543084 2307 68-69 0.3770015339723898 2307 70-71 0.42609589565176265 2307 72-73 0.2940882138417251 2307 74-75 0.2669926760496466 2307 76-77 -0.1611173641194057 2307 78-79 0.06417453488493408 2307 80-81 0.18603199861091468 2307 82-83 0.25853896548651534 2307 84-85 0.3024796017319815 2307 86-87 0.10669079247549718 2307 88-89 0.1336122716000432 2307 90-91 0.09555893019970085 2307 92-93 0.07100072791525491 2307 94-95 0.12244634769373164 2307 96-97 0.291730870407477 2307 98-99 0.11682167013578493 2307 100-101 0.06345446357477513 2201 1 0.10255809601552812 2201 2 0.12326474111819152 2201 3 0.08050513816405669 2201 4 0.12300412516572123 2201 5 0.15582383033605396 2201 6 0.15052130285412346 2201 7 0.10329781357752665 2201 8 0.19247926453207498 2201 9 0.13257781590272089 2201 10-11 0.08756384012453111 2201 12-13 0.11948121161745462 2201 14-15 0.27824824198285825 2201 16-17 0.270635030864959 2201 18-19 0.25045442524198336 2201 20-21 0.15246333881140828 2201 22-23 0.18913961126493462 2201 24-25 0.231817676749003 2201 26-27 0.2321965515851261 2201 28-29 0.3638751738756909 2201 30-31 0.2770155088890718 2201 32-33 0.4196261920155777 2201 34-35 0.24529812230930048 2201 36-37 0.17715189326253267 2201 38-39 0.23195932725544566 2201 40-41 0.2378753892483303 2201 42-43 0.34046350481909116 2201 44-45 0.36497167471922154 2201 46-47 0.33874471976126586 2201 48-49 0.2254195793598157 2201 50-51 0.1859005504918798 2201 52-53 0.1555270581692625 2201 54-55 0.2555382684523053 2201 56-57 0.2603691247319375 2201 58-59 0.32159261600862976 2201 60-61 0.3003152017609594 2201 62-63 0.18093236679327873 2201 64-65 0.12611563149575034 2201 66-67 0.22982517746406472 2201 68-69 0.33826480004331927 2201 70-71 0.30099496340483256 2201 72-73 0.35833231341018035 2201 74-75 0.24447609225367017 2201 76-77 0.28756414200220703 2201 78-79 0.22621184021608798 2201 80-81 0.22297078634219503 2201 82-83 0.21360431549444314 2201 84-85 0.29970130882949775 2201 86-87 0.3562054484172634 2201 88-89 0.3435402378490693 2201 90-91 0.19672346618180114 2201 92-93 0.09616424781388133 2201 94-95 0.13946912650819598 2201 96-97 0.2227178429036769 2201 98-99 0.26915547480822255 2201 100-101 0.16714085694203362 1101 1 0.044968602756576104 1101 2 0.02954864007991631 1101 3 0.07586340176238338 1101 4 0.07751171853314531 1101 5 0.09065791913196364 1101 6 0.06049050322604188 1101 7 0.1441048994290668 1101 8 0.020059459790090273 1101 9 0.011405977223034824 1101 10-11 0.07255386414436771 1101 12-13 0.11827295576746621 1101 14-15 0.13971771354648155 1101 16-17 0.08610361137015587 1101 18-19 -0.05280084362520654 1101 20-21 -0.010038598272117838 1101 22-23 0.052318559041154344 1101 24-25 0.04735163382323293 1101 26-27 0.00899610218174729 1101 28-29 0.06169861839762092 1101 30-31 0.11089001493277095 1101 32-33 0.14850471750759198 1101 34-35 -0.03852940945886729 1101 36-37 0.022717909069129405 1101 38-39 0.08260776596611663 1101 40-41 0.08018710472950374 1101 42-43 0.12546781482993907 1101 44-45 -0.10853628669406845 1101 46-47 -0.12908516556338867 1101 48-49 0.025916925555385717 1101 50-51 0.0327619909071899 1101 52-53 0.06278313092611398 1101 54-55 0.003472853141595067 1101 56-57 0.050061176878223534 1101 58-59 0.13011069868335312 1101 60-61 0.06953954508964699 1101 62-63 -0.002383532788314824 1101 64-65 0.042214490553547535 1101 66-67 0.15924390712469005 1101 68-69 0.039256586809159444 1101 70-71 0.10011819097340435 1101 72-73 0.04791952091056828 1101 74-75 0.13331292202277467 1101 76-77 0.03215604693015095 1101 78-79 0.0650041170640705 1101 80-81 0.29051446757573274 1101 82-83 0.4601538854618994 1101 84-85 0.46500717431841565 1101 86-87 0.25875440820341566 1101 88-89 0.26570580037812874 1101 90-91 0.2104973407362678 1101 92-93 0.29079271519339045 1101 94-95 0.2666229212548288 1101 96-97 0.2344953107480876 1101 98-99 0.38436472825623724 1101 100-101 0.36936269174755054 2304 1 0.08920979516850025 2304 2 0.05009967892363676 2304 3 0.07014693834774732 2304 4 0.031655801640646075 2304 5 0.05570703837315705 2304 6 0.060473565556662834 2304 7 0.042120369167101046 2304 8 0.08598086900418167 2304 9 0.04144493386259995 2304 10-11 -0.06862443305526966 2304 12-13 0.061613628757527295 2304 14-15 0.10409214895749841 2304 16-17 0.0993545147125161 2304 18-19 0.037482580042365043 2304 20-21 0.05945101329066205 2304 22-23 0.08420089869788683 2304 24-25 0.08598363472657411 2304 26-27 0.027638830275698467 2304 28-29 0.06026879680773334 2304 30-31 0.014959290000525982 2304 32-33 0.07269453232240863 2304 34-35 0.09572843832794575 2304 36-37 0.04538051069514637 2304 38-39 -0.025965517683381734 2304 40-41 0.06883886624711977 2304 42-43 0.05470928953635479 2304 44-45 0.17116391655796548 2304 46-47 -0.024709600974411217 2304 48-49 -0.07622004373097724 2304 50-51 -0.04811255742162501 2304 52-53 0.031876118730998826 2304 54-55 -0.10804758497945244 2304 56-57 -0.023904213817345976 2304 58-59 -0.037658327965246485 2304 60-61 -0.10528684379924158 2304 62-63 0.07170183306534739 2304 64-65 -0.057786920918900364 2304 66-67 -0.11001845737395399 2304 68-69 -0.0032456715467503727 2304 70-71 -0.07060928192632332 2304 72-73 0.266308183963865 2304 74-75 0.026765101336231112 2304 76-77 -0.32264154792079935 2304 78-79 -0.058695916811274174 2304 80-81 -0.019662248774764635 2304 82-83 0.07953904806352341 2304 84-85 0.2687729828098355 2304 86-87 0.2322511900462345 2304 88-89 0.1967440795109212 2304 90-91 -0.04056832096200935 2304 92-93 -0.017935830425393817 2304 94-95 -0.011709565195040739 2304 96-97 0.12695240198567603 2304 98-99 0.18665272509453246 2304 100-101 0.37070641351809996 2202 1 0.09338740617538122 2202 2 0.0655885261198037 2202 3 0.04571917099315215 2202 4 0.07063849915992648 2202 5 0.10190257725162155 2202 6 0.14415503314057077 2202 7 0.07400696495613346 2202 8 0.12813638286701234 2202 9 0.10543909688115605 2202 10-11 0.09910122881673544 2202 12-13 0.09266220078170306 2202 14-15 0.005194921523681728 2202 16-17 0.04215418117072289 2202 18-19 0.054659982585619105 2202 20-21 0.026528353294828833 2202 22-23 0.09012126559386502 2202 24-25 -0.05994715535054951 2202 26-27 0.14990066058630447 2202 28-29 0.05812579076228985 2202 30-31 -0.03528252780227348 2202 32-33 0.14705350668138095 2202 34-35 -0.06463428837624718 2202 36-37 0.07632902018023913 2202 38-39 0.02202906226241197 2202 40-41 0.030379412421808638 2202 42-43 -0.04102346009883462 2202 44-45 -0.02052880806159152 2202 46-47 -0.034701262429486235 2202 48-49 -0.00820878669532732 2202 50-51 0.07768898520918555 2202 52-53 0.16608708391757432 2202 54-55 -0.01879388475014565 2202 56-57 -0.14640160802206026 2202 58-59 0.01084698785714977 2202 60-61 0.019926831414430524 2202 62-63 -0.12590917381395172 2202 64-65 0.01091018143674205 2202 66-67 0.0919869626802381 2202 68-69 0.10902866658123855 2202 70-71 0.06146701575973168 2202 72-73 -0.010310536069489729 2202 74-75 -0.0795138229914727 2202 76-77 -0.4120453419587413 2202 78-79 -0.139410947038499 2202 80-81 0.024138043074302118 2202 82-83 0.2244754666584754 2202 84-85 0.19524577830702583 2202 86-87 0.050648104784606574 2202 88-89 0.09840344995077999 2202 90-91 0.2218978036992354 2202 92-93 0.13024428214495742 2202 94-95 0.00163627595568272 2202 96-97 0.10255798881077283 2202 98-99 -0.13835874040473328 2202 100-101 -0.03337947349461601 1102 1 -0.0012616535695926245 1102 2 -0.0039222385705031115 1102 3 0.028588746081126715 1102 4 -0.020975510702974987 1102 5 0.032360281541102154 1102 6 -0.08098931586998503 1102 7 -0.05481062178570539 1102 8 -0.03070705931139628 1102 9 -0.027815787273880233 1102 10-11 0.05647910917542731 1102 12-13 0.04829250587585676 1102 14-15 -0.04952892980124801 1102 16-17 -0.06983782256255466 1102 18-19 -0.0802990901501417 1102 20-21 -0.04375769612058633 1102 22-23 -0.015773773946037295 1102 24-25 -0.07164983044231121 1102 26-27 0.00120680156780395 1102 28-29 -0.0873321027133045 1102 30-31 -0.062011878137127496 1102 32-33 -0.09626759261610829 1102 34-35 -0.04779848931713104 1102 36-37 -0.06486133889501389 1102 38-39 -0.08976139518962611 1102 40-41 -0.14371230500979948 1102 42-43 -0.1798074978351636 1102 44-45 -0.14581101069254032 1102 46-47 -0.33251389249494423 1102 48-49 -0.3268984836191535 1102 50-51 -0.31455274420421375 1102 52-53 -0.30220797075721606 1102 54-55 -0.42487747979094337 1102 56-57 -0.24447922478142914 1102 58-59 -0.4565042803204875 1102 60-61 -0.2866335126520667 1102 62-63 -0.471753511473338 1102 64-65 -0.2381942114650002 1102 66-67 -0.17703090910201524 1102 68-69 -0.30211540495633926 1102 70-71 -0.232782609021136 1102 72-73 -0.21192441980830523 1102 74-75 -0.3268050561066147 1102 76-77 -0.44026472218500956 1102 78-79 -0.12918114896314847 1102 80-81 -0.05255435290486332 1102 82-83 -0.015466264016161801 1102 84-85 -0.10474526688323493 1102 86-87 -0.024768419992462043 1102 88-89 -0.1259115071039716 1102 90-91 -0.0019667642627254622 1102 92-93 0.04405713923376453 1102 94-95 -0.10683951452698892 1102 96-97 -0.04298642578430645 1102 98-99 -0.11168753327115866 1102 100-101 -0.14944389767512334 2305 1 -0.014744461956482269 2305 2 0.009105009636286354 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-0.21456572514009054 1213 64-65 -0.19591502836439645 1213 66-67 -0.08960311755316752 1213 68-69 -0.03140496251759828 1213 70-71 -0.15376458706487028 1213 72-73 -0.042148049394960196 1213 74-75 0.18655538132718164 1213 76-77 0.3026748831115924 1213 78-79 -0.13758930405712277 1213 80-81 -0.2807619657425562 1213 82-83 -0.25464685522095465 1213 84-85 -0.25208287407633634 1213 86-87 -0.20109442693603796 1213 88-89 -0.5063350500612387 1213 90-91 -0.3334892964362268 1213 92-93 -0.19965496521599135 1213 94-95 -0.09475330091529344 1213 96-97 -0.21020673734564355 1213 98-99 -0.21942141223326672 1213 100-101 -0.03280798080058389 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 5.0 10 12.0 11 12.0 12 12.0 13 36.0 14 118.0 15 277.0 16 592.0 17 1069.0 18 1595.0 19 2445.0 20 3287.0 21 4371.0 22 5714.0 23 7380.0 24 9402.0 25 11878.0 26 15548.0 27 20237.0 28 28082.0 29 35875.0 30 37074.0 31 43240.0 32 58377.0 33 82952.0 34 128107.0 35 219632.0 36 432775.0 37 747677.0 38 282496.0 39 551.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.29633560560169 25.82564722448153 24.129276347665783 14.74874082225099 2 29.1051113566853 24.19207366002852 33.488167349128545 13.214647634157636 3 36.5365540713743 19.995726144941468 27.386377132857735 16.0813426508265 4 28.176266062812232 20.375226193194898 34.18844220414205 17.260065539850817 5 31.249753534204867 18.917063686761463 33.76301683304063 16.07016594599304 6 25.36616792689022 19.802919072096405 38.570223263619816 16.26068973739356 7 31.48567288601129 21.234850951243335 29.54829124691058 17.731184915834795 8 27.97714631585222 28.96910809187328 26.12853821710083 16.925207375173674 9 29.986014499066872 25.612175181009068 26.2959056872842 18.105904632639866 10-11 23.985271662623862 29.306869402933742 29.98766524671799 16.7201936877244 12-13 23.191089260093296 27.723241941481884 29.83709405918575 19.248574739239068 14-15 22.151428584529743 28.421403777460874 30.585992489098185 18.841175148911194 16-17 22.04181435508499 28.413310528560228 31.359092639041098 18.185782477313683 18-19 20.633749535727222 28.410811480032834 32.33956796265642 18.615871021583523 20-21 21.491954897905842 28.832439942590664 31.9587955044639 17.716809655039594 22-23 20.559200835096235 30.680524104049546 30.908992531042706 17.85128252981152 24-25 20.699004667719322 28.707471170946654 31.710755013172744 18.882769148161284 26-27 20.68744468849016 28.880586749081772 30.747421853147657 19.68454670928041 28-29 21.198855481628552 29.772449021702744 30.668506944603656 18.36018855206504 30-31 20.055277119261934 29.332020377562674 31.356157976550215 19.256544526625184 32-33 19.89160090424288 30.27640852336037 30.887276862478963 18.944713709917785 34-35 20.132908112966163 29.32145100718534 31.746147109128174 18.79949377072032 36-37 20.93521732551368 29.237744345042945 31.03029580480826 18.796742524635114 38-39 21.477008464960438 29.92110512888883 30.316301692006213 18.285584714144516 40-41 21.40538235442469 29.95873130872191 29.948437062953097 18.687449273900302 42-43 20.151937565055505 29.988719891050653 31.27327668823338 18.58606585566046 44-45 19.912941323203846 29.182402280234847 31.58222473299132 19.322431663569983 46-47 20.991612525887724 29.086764261835558 30.52560938898204 19.396013823294677 48-49 20.27976693654723 30.407613424465886 30.70099475898412 18.61162488000277 50-51 21.23100830787657 29.389308251659234 30.450582395078552 18.92910104538564 52-53 20.080213738224785 29.28383800704192 29.86601008758948 20.769938167143824 54-55 21.034454850245197 29.38203575186235 30.463712205922512 19.11979719196994 56-57 20.220498040807154 29.41930395346544 30.481440975446468 19.878757030280937 58-59 20.20023689456266 29.33092647968535 30.966076753654875 19.502759872097116 60-61 20.701878595720068 29.54329468780902 30.878856862226762 18.875969854244158 62-63 19.839307744881555 29.62030929269775 30.449907591865333 20.090475370555357 64-65 20.63988312569971 29.428681021155267 30.428312120595862 19.503123732549156 66-67 20.008758796055332 30.613574299582925 29.792219345933464 19.585447558428278 68-69 19.84843301603257 31.151550475400857 29.60323035962078 19.39678614894579 70-71 19.570553371057674 31.762917689205043 29.385110404337066 19.281418535400217 72-73 19.588605802752458 31.406266730529765 29.488654250354184 19.516473216363593 74-75 19.865604508006427 30.84582213542867 29.664754166583553 19.62381918998135 76-77 19.934416637318776 30.571628110876325 29.852719448846628 19.641235802958267 78-79 19.91519380833177 30.557352858230956 29.92463098484595 19.602822348591324 80-81 19.566254607550317 30.979796134025406 29.754165395150533 19.699783863273744 82-83 19.37092758261763 30.69780771541111 29.80216247942297 20.129102222548294 84-85 19.530888002118466 30.383674049584503 30.11483052839009 19.970607419906937 86-87 19.70719076047818 30.395718462742323 30.26067661696561 19.636414159813885 88-89 19.939107672208607 30.883241403297834 29.60403937297273 19.573611551520827 90-91 20.129944572818165 30.525487469843387 29.45234532597117 19.892222631367282 92-93 19.698837364472688 30.78253308991956 29.66969925008564 19.848930295522113 94-95 19.330480998959036 31.10858130757978 29.639927178344273 19.92101051511691 96-97 19.54038195646641 31.40389293846002 29.324777643004378 19.73094746206919 98-99 19.983165786053693 30.811105536901827 29.055779899645888 20.14994877739859 100-101 19.96453407175591 30.706963799669325 28.75688803217522 20.57161409639955 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 30.0 1 26.0 2 23.5 3 45.0 4 60.5 5 45.5 6 43.5 7 45.0 8 35.5 9 40.5 10 44.0 11 46.0 12 53.5 13 60.0 14 64.0 15 79.5 16 105.0 17 157.5 18 248.0 19 395.5 20 701.0 21 1194.0 22 1913.0 23 3052.5 24 4612.0 25 6861.5 26 10568.5 27 15517.5 28 21120.0 29 26908.0 30 32421.5 31 37794.0 32 44629.0 33 54307.5 34 66026.0 35 81807.5 36 101615.0 37 121889.5 38 141989.0 39 153065.0 40 154711.0 41 145339.5 42 128382.0 43 117681.5 44 106903.0 45 97108.0 46 92956.0 47 83708.5 48 67336.5 49 53932.0 50 44325.0 51 35978.0 52 28881.0 53 23090.0 54 18744.0 55 14739.0 56 10819.0 57 7663.0 58 5513.5 59 3966.5 60 2856.5 61 2010.0 62 1407.5 63 933.0 64 627.0 65 452.5 66 332.0 67 234.0 68 169.5 69 124.5 70 81.5 71 55.5 72 40.0 73 33.0 74 18.5 75 11.0 76 11.5 77 8.0 78 4.5 79 5.5 80 4.5 81 3.0 82 2.0 83 0.0 84 0.5 85 1.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1566834645524869 2 0.0 3 0.005915179083193096 4 0.007886905444257462 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.585410142010152E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.6048935497035532E-4 24-25 2.292705071005076E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.565926734316751E-4 40-41 0.0 42-43 0.0 44-45 9.170820284020305E-5 46-47 8.483008762718782E-4 48-49 0.0023156321217151265 50-51 3.668328113608122E-4 52-53 0.001444404194733198 54-55 0.0022239239188749237 56-57 0.003897598620708629 58-59 0.005181513460471472 60-61 0.0011692795862125887 62-63 7.107385720115736E-4 64-65 0.0011692795862125887 66-67 0.00756592673431675 68-69 0.00660299060449462 70-71 0.008551789914848933 72-73 0.005662981525382538 74-75 0.008093248900647918 76-77 0.004310285533489543 78-79 0.004264431432069441 80-81 0.0015819664989935023 82-83 0.0 84-85 3.4390576065076143E-4 86-87 0.0011234254847924871 88-89 0.004379066685619695 90-91 0.006006887286033299 92-93 0.008299592357038376 94-95 0.007336656227216244 96-97 0.009858631805321826 98-99 0.006969823415855432 100-101 0.0066717717566247715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2180830.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.3045474674163 #Duplication Level Percentage of deduplicated Percentage of total 1 92.55802260897853 61.370178035672076 2 3.6229781675083554 4.804398557619413 3 1.154380062516113 2.296219429516158 4 0.6120913934690332 1.6233777141065795 5 0.39886594173285644 1.322331288338094 6 0.2829404125008444 1.1256141606667551 7 0.21262394085231912 0.9868553925266196 8 0.15756314092069096 0.8357722205032925 9 0.13044951770922603 0.7784456615147646 >10 0.7390607593258381 9.244746326984563 >50 0.061249611215975025 2.8231932441136185 >100 0.06590592073512323 9.112922990848244 >500 0.003036538274915847 1.2758353155636417 >1k 7.62797787615417E-4 0.9165975689462514 >5k 0.0 0.0 >10k+ 6.918647261724849E-5 1.4835120930798882 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 32339 1.482875785824663 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2498 0.11454354534741361 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2285 0.10477662174493198 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2249 0.10312587409380831 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.878115213015229E-4 0.0 0.0 2 6.878115213015229E-4 0.0 0.0 3 8.712279269819289E-4 0.0 0.0 4 9.62936129822132E-4 0.0 0.0 5 0.001146352535502538 0.0 0.0 6 0.0013297689411829441 0.0 0.0 7 0.0016048935497035533 0.0 0.0 8 0.001834164056804061 0.0 4.585410142010152E-5 9 0.002017580462484467 0.0 4.585410142010152E-5 10-11 0.002888808389466396 0.0 4.585410142010152E-5 12-13 0.004012233874258883 0.0 4.585410142010152E-5 14-15 0.005571273322542335 0.0 4.585410142010152E-5 16-17 0.0064654283002343145 0.0 9.170820284020305E-5 18-19 0.007909832494967513 0.0 1.3756230426030457E-4 20-21 0.009835704754611777 0.0 1.6048935497035534E-4 22-23 0.011784504064966091 0.0 1.834164056804061E-4 24-25 0.01419184438952142 0.0 1.834164056804061E-4 26-27 0.016736747018337054 0.0 2.2927050710050761E-4 28-29 0.01980897181348386 0.0 2.2927050710050761E-4 30-31 0.030492977444367513 0.0 2.2927050710050761E-4 32-33 0.034000816203005275 0.0 2.2927050710050761E-4 34-35 0.03789841482371391 0.0 2.2927050710050761E-4 36-37 0.041979429850102945 0.0 2.2927050710050761E-4 38-39 0.04594580962294172 0.0 2.2927050710050761E-4 40-41 0.05046243861282172 0.0 2.2927050710050761E-4 42-43 0.0558273684789736 0.0 2.2927050710050761E-4 44-45 0.06112351719299533 0.0 2.2927050710050761E-4 46-47 0.06735967498612913 0.0 2.2927050710050761E-4 48-49 0.07391681148920365 0.0 2.2927050710050761E-4 50-51 0.08010711518091736 0.0 2.2927050710050761E-4 52-53 0.08684766808967229 0.0 2.521975578105584E-4 54-55 0.09457408417895938 0.0 2.7512460852060914E-4 56-57 0.1027131871810274 0.0 2.7512460852060914E-4 58-59 0.11154010170439696 0.0 2.7512460852060914E-4 60-61 0.12020652687279615 0.0 2.980516592306599E-4 62-63 0.13098224070651998 0.0 3.439057606507614E-4 64-65 0.14244576606154538 0.0 3.668328113608122E-4 66-67 0.15544540381414418 0.0 4.126869127809137E-4 68-69 0.1691557801387545 0.0 4.356139634909645E-4 70-71 0.18557154844715085 0.0 4.5854101420101523E-4 72-73 0.20327123159531002 0.0 4.5854101420101523E-4 74-75 0.22241531893820243 0.0 4.5854101420101523E-4 76-77 0.24527358849612302 0.0 4.5854101420101523E-4 78-79 0.2712499369506105 0.0 4.5854101420101523E-4 80-81 0.2993585011211328 0.0 4.5854101420101523E-4 82-83 0.3318919860786948 0.0 4.5854101420101523E-4 84-85 0.36887331887400665 0.0 4.5854101420101523E-4 86-87 0.4089956576165955 0.0 4.5854101420101523E-4 88-89 0.45684441244847146 0.0 4.5854101420101523E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 5240 0.0 68.626686 3 CGGAAGA 5280 0.0 68.013695 4 GATCGGA 5455 0.0 66.19622 1 ATCGGAA 5595 0.0 64.44216 2 GAAGAGC 5940 0.0 60.29115 6 ACGCGCC 25 0.0050202864 57.004566 2 GGAAGAG 7015 0.0 51.187366 5 ATGCCGT 3925 0.0 44.231968 48-49 CGTATGC 3910 0.0 44.158695 46-47 TATGCCG 3940 0.0 43.82246 48-49 TCGTATG 4025 0.0 43.781094 44-45 TGCCGTC 3945 0.0 43.76692 50-51 GCCGTCT 3980 0.0 43.74007 50-51 TCTCGTA 3960 0.0 43.66011 42-43 CTCGTAT 3945 0.0 43.404713 44-45 TTGTGTT 24005 0.0 42.98768 1 CCGTCTT 4035 0.0 42.790215 52-53 AATCTCG 4085 0.0 42.78824 40-41 CACTCCG 4205 0.0 42.583763 30-31 CGTCTTC 4105 0.0 42.581234 52-53 >>END_MODULE