FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_30245-1.34100000035771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_30245-1.34100000035771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180830
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT323391.482875785824663TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC24980.11454354534741361No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC22850.10477662174493198No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG22490.10312587409380831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG52400.068.6266863
CGGAAGA52800.068.0136954
GATCGGA54550.066.196221
ATCGGAA55950.064.442162
GAAGAGC59400.060.291156
ACGCGCC250.005020286457.0045662
GGAAGAG70150.051.1873665
ATGCCGT39250.044.23196848-49
CGTATGC39100.044.15869546-47
TATGCCG39400.043.8224648-49
TCGTATG40250.043.78109444-45
TGCCGTC39450.043.7669250-51
GCCGTCT39800.043.7400750-51
TCTCGTA39600.043.6601142-43
CTCGTAT39450.043.40471344-45
TTGTGTT240050.042.987681
CCGTCTT40350.042.79021552-53
AATCTCG40850.042.7882440-41
CACTCCG42050.042.58376330-31
CGTCTTC41050.042.58123452-53
Produced by FastQC (version 0.11.2)