##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l01n01_30230-1.34100000035714.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2184150 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.681983380262345 33.0 33.0 33.0 33.0 33.0 2 32.708987477966254 33.0 33.0 33.0 33.0 33.0 3 32.73508458668132 33.0 33.0 33.0 33.0 33.0 4 36.67671039076986 37.0 37.0 37.0 37.0 37.0 5 36.67605750520798 37.0 37.0 37.0 37.0 37.0 6 36.564987294828654 37.0 37.0 37.0 37.0 37.0 7 36.5010658608612 37.0 37.0 37.0 37.0 37.0 8 36.42222283268091 37.0 37.0 37.0 37.0 37.0 9 36.43190119726209 37.0 37.0 37.0 37.0 37.0 10-11 36.364191561934845 37.0 37.0 37.0 37.0 37.0 12-13 36.34289197170524 37.0 37.0 37.0 37.0 37.0 14-15 38.16547856145411 40.0 37.0 40.0 37.0 40.0 16-17 38.09859190073942 40.0 37.0 40.0 37.0 40.0 18-19 38.136865599890115 40.0 37.0 40.0 37.0 40.0 20-21 38.07961403749742 40.0 37.0 40.0 37.0 40.0 22-23 37.94463086326489 40.0 37.0 40.0 33.0 40.0 24-25 37.93033422612916 40.0 37.0 40.0 33.0 40.0 26-27 37.80571870063869 40.0 37.0 40.0 33.0 40.0 28-29 37.67509191218552 40.0 37.0 40.0 33.0 40.0 30-31 37.64405283519905 40.0 37.0 40.0 33.0 40.0 32-33 37.53387793878626 40.0 37.0 40.0 33.0 40.0 34-35 37.414485497790906 40.0 37.0 40.0 33.0 40.0 36-37 37.293957603644444 40.0 37.0 40.0 33.0 40.0 38-39 37.15838861799784 40.0 37.0 40.0 33.0 40.0 40-41 37.08357187006387 38.5 37.0 40.0 33.0 40.0 42-43 36.84939221207335 37.0 37.0 40.0 33.0 40.0 44-45 36.683458324748756 37.0 37.0 40.0 33.0 40.0 46-47 36.6669379392441 37.0 37.0 40.0 33.0 40.0 48-49 36.44474532426802 37.0 37.0 40.0 33.0 40.0 50-51 36.315091454341506 37.0 37.0 40.0 33.0 40.0 52-53 36.17089851887462 37.0 37.0 40.0 33.0 40.0 54-55 35.94922235194469 37.0 37.0 40.0 33.0 40.0 56-57 35.73495845065587 37.0 35.0 40.0 30.0 40.0 58-59 35.51441865256507 37.0 33.0 40.0 27.0 40.0 60-61 35.27654350662729 37.0 33.0 40.0 27.0 40.0 62-63 34.99966966554495 37.0 33.0 37.0 27.0 40.0 64-65 34.6982807957329 37.0 33.0 37.0 27.0 40.0 66-67 34.24381361170249 37.0 33.0 37.0 27.0 40.0 68-69 33.94417988691253 37.0 33.0 37.0 27.0 38.5 70-71 33.55194080076918 37.0 33.0 37.0 27.0 37.0 72-73 33.03818441956825 37.0 33.0 37.0 27.0 37.0 74-75 32.55239864478172 35.0 33.0 37.0 22.0 37.0 76-77 29.745620264175997 33.0 27.0 33.0 22.0 37.0 78-79 32.24607101160635 33.0 33.0 37.0 24.5 37.0 80-81 32.78295675663301 37.0 33.0 37.0 27.0 37.0 82-83 32.61840761852437 37.0 33.0 37.0 22.0 37.0 84-85 32.49523315706339 33.0 33.0 37.0 22.0 37.0 86-87 32.373679005562806 33.0 33.0 37.0 22.0 37.0 88-89 32.28960991690131 33.0 33.0 37.0 22.0 37.0 90-91 32.17549344138452 33.0 33.0 37.0 22.0 37.0 92-93 32.02872444658105 33.0 33.0 37.0 22.0 37.0 94-95 31.77484444749674 33.0 33.0 37.0 22.0 37.0 96-97 31.606999061419774 33.0 33.0 37.0 18.5 37.0 98-99 31.451088753061832 33.0 33.0 37.0 15.0 37.0 100-101 29.73138154430785 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1835289299786922 2306 2 0.1591469963883938 2306 3 0.14399133920487373 2306 4 0.1596859215599693 2306 5 0.19326150243931295 2306 6 0.21994152604492712 2306 7 0.21812746807385253 2306 8 0.2090625856282884 2306 9 0.15013146813070932 2306 10-11 0.1747102378395482 2306 12-13 0.21829881769446047 2306 14-15 0.46240028997137017 2306 16-17 0.29459153724269527 2306 18-19 0.3709253989464969 2306 20-21 0.4238640790030743 2306 22-23 0.5037494688488309 2306 24-25 0.5397895701490043 2306 26-27 0.38696248402465727 2306 28-29 0.4089907322712918 2306 30-31 0.4008781934737655 2306 32-33 0.5738264392900092 2306 34-35 0.5495213799798861 2306 36-37 0.4332040711459726 2306 38-39 0.4270260125150429 2306 40-41 0.4747133518413946 2306 42-43 0.5457815665410308 2306 44-45 0.5425909317208806 2306 46-47 0.47105927640472345 2306 48-49 0.5734845216682345 2306 50-51 0.4258305695717439 2306 52-53 0.4528166677490759 2306 54-55 0.49001376310374667 2306 56-57 0.5686103729256615 2306 58-59 0.5118438163438839 2306 60-61 0.5710003537323516 2306 62-63 0.5001231400467603 2306 64-65 0.5280483947152064 2306 66-67 0.3742046552627869 2306 68-69 0.47698162356663687 2306 70-71 0.44697272839235325 2306 72-73 0.33250037421169765 2306 74-75 0.40784571819964555 2306 76-77 -0.0657782564444993 2306 78-79 0.242516567895656 2306 80-81 0.3866086132915356 2306 82-83 0.46956590720375857 2306 84-85 0.4119260795763253 2306 86-87 0.27717042341433995 2306 88-89 0.2919211599875595 2306 90-91 0.3600249765555077 2306 92-93 0.5016052981031294 2306 94-95 0.3255075080403991 2306 96-97 0.33954392280275414 2306 98-99 0.24024085419528163 2306 100-101 0.23493734477357364 2307 1 0.13792050996269012 2307 2 0.10391171549373013 2307 3 0.13012389611838415 2307 4 0.1424579918552169 2307 5 0.1349808432674564 2307 6 0.142989526879866 2307 7 0.17474855664940492 2307 8 0.143198586655906 2307 9 0.2192097728879716 2307 10-11 0.15743798557090116 2307 12-13 0.2146247280297473 2307 14-15 0.3574921263237698 2307 16-17 0.3020285289548852 2307 18-19 0.3232217760703051 2307 20-21 0.3318815457728519 2307 22-23 0.3121329898199221 2307 24-25 0.3213152899474352 2307 26-27 0.3460271675381392 2307 28-29 0.523111800776249 2307 30-31 0.4958007343575659 2307 32-33 0.48909741908293825 2307 34-35 0.39011335548840265 2307 36-37 0.47807790738598044 2307 38-39 0.41155476163527993 2307 40-41 0.38274068665370464 2307 42-43 0.2745485496094844 2307 44-45 0.35220829251791486 2307 46-47 0.24319580461997248 2307 48-49 0.2586494751981263 2307 50-51 0.37606971071084416 2307 52-53 0.34933853399328285 2307 54-55 0.41516917641082074 2307 56-57 0.2759150325149875 2307 58-59 0.44177653301933617 2307 60-61 0.2994661106212462 2307 62-63 0.25617960933474393 2307 64-65 0.2722399645301081 2307 66-67 0.27582696145442753 2307 68-69 0.3114176130871087 2307 70-71 0.42713143996719793 2307 72-73 0.2481670792355004 2307 74-75 -0.05468381902595354 2307 76-77 -0.15406144451132775 2307 78-79 0.16355784374324145 2307 80-81 0.30286137968854376 2307 82-83 0.24967179966283481 2307 84-85 0.44160212679307165 2307 86-87 0.11976360449659751 2307 88-89 0.29631912911096236 2307 90-91 0.22272705399588943 2307 92-93 0.13289224472337224 2307 94-95 0.05448924647505393 2307 96-97 0.2639495321326386 2307 98-99 0.1074337272608119 2307 100-101 0.022660028024795054 2201 1 0.14701790175558926 2201 2 0.12148891993340527 2201 3 0.12240540948793921 2201 4 0.11215652742223625 2201 5 0.12812421151029696 2201 6 0.1312912249406608 2201 7 0.12535193335420303 2201 8 0.17782153924166977 2201 9 0.19072534405629682 2201 10-11 0.19742169425894218 2201 12-13 0.17880451120685592 2201 14-15 0.3106477146164437 2201 16-17 0.18147454292306975 2201 18-19 0.2558724526984122 2201 20-21 0.23805179405407983 2201 22-23 0.2798350410783641 2201 24-25 0.24274071397721997 2201 26-27 0.2597107734737918 2201 28-29 0.2950071267481036 2201 30-31 0.21515626609102156 2201 32-33 0.3250687736826663 2201 34-35 0.22087678290234436 2201 36-37 0.2135582990915026 2201 38-39 0.2357286947218924 2201 40-41 0.29300740756210786 2201 42-43 0.28622305032399 2201 44-45 0.2794700273829278 2201 46-47 0.2795189789016206 2201 48-49 0.4573803062084849 2201 50-51 0.3405640771801828 2201 52-53 0.18538505035521524 2201 54-55 0.2310496353861211 2201 56-57 0.29827581335801057 2201 58-59 0.23311323172057286 2201 60-61 0.3601401299873004 2201 62-63 0.24904722786249067 2201 64-65 0.27966234513485944 2201 66-67 0.52238744960502 2201 68-69 0.3173842043101516 2201 70-71 0.2803262689632362 2201 72-73 0.3073599421251032 2201 74-75 0.2743294354376431 2201 76-77 0.018761596827957305 2201 78-79 0.2629501922132107 2201 80-81 0.38838356446559175 2201 82-83 0.5473463573750053 2201 84-85 0.6410988248428993 2201 86-87 0.46871022805799356 2201 88-89 0.5470763055716716 2201 90-91 0.4735387227342045 2201 92-93 0.26205178276665464 2201 94-95 0.45833543043409364 2201 96-97 0.5241396663370921 2201 98-99 0.5131049327583916 2201 100-101 0.30123942687475846 1101 1 0.08657301352848634 1101 2 0.051614427458815726 1101 3 0.030725430798838715 1101 4 0.12203584049564853 1101 5 0.05156237683818432 1101 6 0.08925612783357195 1101 7 0.11378890810493658 1101 8 0.00834776268720816 1101 9 0.048374576418197535 1101 10-11 0.038513306329562624 1101 12-13 0.06950734227401512 1101 14-15 0.15066766151991118 1101 16-17 0.17950214857476254 1101 18-19 0.045422420361923344 1101 20-21 0.1427212464089891 1101 22-23 0.25953868791675205 1101 24-25 0.17295686275010524 1101 26-27 0.14596548004242038 1101 28-29 0.27455688748143814 1101 30-31 0.05612882044589185 1101 32-33 0.11458741036835818 1101 34-35 0.16159871857970387 1101 36-37 0.04404925697129869 1101 38-39 -0.05533798371939014 1101 40-41 0.01217182349876822 1101 42-43 0.07989675757141157 1101 44-45 0.00490442774734845 1101 46-47 0.086153801636776 1101 48-49 0.09248327145966329 1101 50-51 0.05499445522995927 1101 52-53 0.06918428261510456 1101 54-55 0.11436593341104384 1101 56-57 -0.02092258576017514 1101 58-59 -0.17585075047877297 1101 60-61 -0.07723886321868889 1101 62-63 -0.15218018869583005 1101 64-65 0.13562174485081613 1101 66-67 0.061139788863258104 1101 68-69 0.10194613457818491 1101 70-71 -0.023095889878121056 1101 72-73 -0.020000541030640306 1101 74-75 0.08814721272189985 1101 76-77 -0.035019265278144474 1101 78-79 0.28315488404900435 1101 80-81 0.3511065499492929 1101 82-83 0.49493181735310543 1101 84-85 0.4946337897769837 1101 86-87 0.35964636561585195 1101 88-89 0.2876355533250958 1101 90-91 0.25143458672453534 1101 92-93 0.1965984555771101 1101 94-95 0.28129850155773894 1101 96-97 0.2005990851430859 1101 98-99 0.27578836834729614 1101 100-101 0.172469253732487 2304 1 -0.009846681861880313 2304 2 0.07122404945342709 2304 3 0.022079677329337244 2304 4 0.04171723225843493 2304 5 0.04373446491309352 2304 6 0.06224447453377735 2304 7 0.058565759581895804 2304 8 0.059661800918476615 2304 9 0.14554023590321918 2304 10-11 0.0032516293398217044 2304 12-13 -0.003927990634345235 2304 14-15 0.0641576320582331 2304 16-17 0.19937020634377944 2304 18-19 0.024666124220374286 2304 20-21 0.04259999695816674 2304 22-23 0.03384134205216327 2304 24-25 0.03552246774825818 2304 26-27 0.0794616099666996 2304 28-29 0.0632571062904006 2304 30-31 -0.046013073716046904 2304 32-33 0.07164736423757034 2304 34-35 0.054485300809652415 2304 36-37 0.07994262098670646 2304 38-39 -0.02818822880264804 2304 40-41 0.09553001332846378 2304 42-43 0.042989436947372894 2304 44-45 0.2126219122196744 2304 46-47 0.2044825244041064 2304 48-49 0.18642252182362995 2304 50-51 0.05767007310682004 2304 52-53 0.07213771894944898 2304 54-55 0.008909196636494698 2304 56-57 0.009678228958598822 2304 58-59 0.10908397833595984 2304 60-61 -0.0029874385914823165 2304 62-63 0.04949891698717579 2304 64-65 0.025485705335988484 2304 66-67 -0.08638504250740198 2304 68-69 -0.15311947580403995 2304 70-71 -0.07930970886970812 2304 72-73 -0.1336420195417034 2304 74-75 0.07613829453404009 2304 76-77 -0.3645652582314618 2304 78-79 -0.022933620512390007 2304 80-81 0.1555696011712584 2304 82-83 0.3440062673156348 2304 84-85 0.201717568194141 2304 86-87 0.19158558152171423 2304 88-89 0.35191674113898586 2304 90-91 0.26961217840462837 2304 92-93 0.04265324503809609 2304 94-95 0.04563414932701093 2304 96-97 0.010871422608520476 2304 98-99 0.2523805480807617 2304 100-101 0.19453401558392258 2202 1 0.06382484952556666 2202 2 0.04226513301999546 2202 3 0.08993053698547726 2202 4 0.03211107472144903 2202 5 0.061884205017051386 2202 6 0.06575357089523237 2202 7 0.13652707505570305 2202 8 0.005174595800525594 2202 9 0.04203413834224534 2202 10-11 0.0609560475725317 2202 12-13 0.08168426446362531 2202 14-15 0.13573949931133455 2202 16-17 -0.05301134103726923 2202 18-19 -0.05240629681121334 2202 20-21 0.06500903334360686 2202 22-23 0.1318881233396425 2202 24-25 -0.025733779752748376 2202 26-27 -0.0330328922324199 2202 28-29 0.0407171222840077 2202 30-31 0.10703470717160002 2202 32-33 0.03582794200646333 2202 34-35 -0.003404293352680554 2202 36-37 0.017557325105059363 2202 38-39 0.08197370642326263 2202 40-41 -0.038001074236511556 2202 42-43 -0.03650776417217827 2202 44-45 -0.013440162950260515 2202 46-47 -0.1158301005459137 2202 48-49 -0.22843953335430456 2202 50-51 0.09166197634552731 2202 52-53 -0.08774482803175943 2202 54-55 -0.11931926198005272 2202 56-57 0.005515643992588082 2202 58-59 -0.15752013002069987 2202 60-61 -0.09903576925921698 2202 62-63 -0.2655788019252654 2202 64-65 -0.06947905875838245 2202 66-67 0.048259099835661345 2202 68-69 0.03552456657900649 2202 70-71 0.10047087889518735 2202 72-73 0.07195824035433418 2202 74-75 0.06164759081553228 2202 76-77 -0.41468820905113546 2202 78-79 -0.024871595322466078 2202 80-81 0.2522946611472676 2202 82-83 0.24892205100216103 2202 84-85 0.3149667685140187 2202 86-87 0.29761516968644486 2202 88-89 0.31394318056322135 2202 90-91 0.2784823803238581 2202 92-93 0.2314749663495732 2202 94-95 0.29785925928302603 2202 96-97 0.15057704036141928 2202 98-99 0.16355607786884718 2202 100-101 0.29062108075785176 1102 1 0.07726628240999389 1102 2 0.06527292387676908 1102 3 0.022686736313808353 1102 4 0.026432456043750108 1102 5 0.03206116714828511 1102 6 -0.035289431468029875 1102 7 0.005474461330678082 1102 8 -0.06313369145480863 1102 9 -0.053680325061080225 1102 10-11 0.0037520492460316746 1102 12-13 -0.012388578351043122 1102 14-15 -0.06005456878234128 1102 16-17 -0.1829840899998132 1102 18-19 -0.17336347536770091 1102 20-21 -0.22536761896255086 1102 22-23 -0.1748282962584895 1102 24-25 -0.10826869161090968 1102 26-27 -0.12888858611620435 1102 28-29 -0.10564446845290831 1102 30-31 -0.11099926051161191 1102 32-33 -0.11012963231198114 1102 34-35 -0.13991310753679187 1102 36-37 -0.09205041826027838 1102 38-39 -0.11111210607602828 1102 40-41 -0.1351022875295982 1102 42-43 0.01969461718404375 1102 44-45 -0.3169760133296293 1102 46-47 -0.24105020073022132 1102 48-49 -0.18374285662397227 1102 50-51 -0.1912319481430913 1102 52-53 -0.1151482903834875 1102 54-55 -0.2141259605338277 1102 56-57 -0.24947955032653368 1102 58-59 -0.1836855359028533 1102 60-61 -0.333580812623417 1102 62-63 -0.2498014689085366 1102 64-65 -0.13198660650423477 1102 66-67 -0.22025057371725865 1102 68-69 -0.23748571192434298 1102 70-71 -0.19261289825691108 1102 72-73 -0.18966981125301885 1102 74-75 -0.3541679614985469 1102 76-77 -0.5712992428944332 1102 78-79 -0.22629351148877674 1102 80-81 -0.21539533285976376 1102 82-83 -0.06479867674753592 1102 84-85 -0.06633246144629368 1102 86-87 -0.012013831749605686 1102 88-89 -0.08191941088606569 1102 90-91 -0.13369408427805496 1102 92-93 -0.18624872396104308 1102 94-95 -0.32478488287466334 1102 96-97 -0.18982878525455504 1102 98-99 -0.3121577070854151 1102 100-101 -0.3174743996406235 2305 1 0.03410593888354185 2305 2 0.10195670033925808 2305 3 0.06221971996698983 2305 4 -0.03199261115845786 2305 5 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84-85 -0.5015917143052775 1213 86-87 -0.41312109960009735 1213 88-89 -0.8342662464208317 1213 90-91 -0.49617913783441026 1213 92-93 -0.3397541585669259 1213 94-95 -0.23504606355777113 1213 96-97 -0.3987226973253364 1213 98-99 -0.4898963554530802 1213 100-101 -0.14040259260513466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 7.0 10 10.0 11 15.0 12 19.0 13 82.0 14 245.0 15 724.0 16 1460.0 17 2527.0 18 3155.0 19 3962.0 20 4911.0 21 6006.0 22 7841.0 23 9592.0 24 12079.0 25 15226.0 26 19811.0 27 26159.0 28 37760.0 29 55504.0 30 57078.0 31 56322.0 32 74653.0 33 106888.0 34 165649.0 35 288712.0 36 510435.0 37 609041.0 38 108222.0 39 52.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.55704922031983 21.938254723340332 23.697086253178927 12.807609803160908 2 35.29551541789712 20.80598859968409 31.58249204496028 12.316003937458508 3 41.89872716425346 15.294587368894346 27.839246966947663 14.967438499904533 4 35.87053473310964 14.407399742120763 31.625280223886065 18.096785300883532 5 35.62374379049058 13.298995032392464 34.90410457157246 16.17315660554449 6 29.775335943044208 13.928622118444245 39.61449534143717 16.681546597074377 7 36.85158986333356 17.340475699929033 27.6290547810361 18.1788796557013 8 33.60039374585079 27.560515532358128 21.160130943387585 17.678959778403495 9 34.644873291669526 25.44774855206831 21.306595243000707 18.60078291326145 10-11 30.147723370647622 26.824256575784627 26.589817549160998 16.43820250440675 12-13 28.318047825938653 25.168767064708714 26.30545665486726 20.207728454485373 14-15 26.958862715472836 25.74504956161436 26.063869239750016 21.232218483162786 16-17 27.136712222145913 26.319460659753226 26.695007211043198 19.848819907057667 18-19 24.63839479889202 26.39445550900808 27.861021449991984 21.106128242107914 20-21 24.99077444314722 27.232951033582857 28.425245518851728 19.351029004418194 22-23 24.30531200753063 28.24717303696731 27.339152236160036 20.108362719342022 24-25 25.198143533247965 24.139722152655334 29.391447195223403 21.270687118873298 26-27 22.52400247235767 24.146647437218142 29.52446031637021 23.80488977405398 28-29 23.097291852665798 27.392601240757276 27.07265984479088 22.43744706178605 30-31 20.26561820387794 27.041549344138456 29.01538355882151 23.6774488931621 32-33 20.983586292150264 26.49405947393723 28.22395439873635 24.298399835176156 34-35 21.860769635785086 26.755763111507907 28.88654625369137 22.496920999015636 36-37 20.340315454524642 29.42563468626239 29.536753428107044 20.697296431105922 38-39 20.22549409675309 27.531972582305965 27.24602440231542 24.996508918625526 40-41 23.15486573724332 26.643820250440676 25.42764919991759 24.773664812398415 42-43 23.272714786072385 28.375020030675547 26.942792390632516 21.409472792619553 44-45 20.760160154202218 28.508729960694 26.66638432887926 24.064725556224516 46-47 22.47033644450315 28.104895359434316 26.6292892856824 22.795478910380133 48-49 21.413871002007237 27.62312187742663 28.93945595797987 22.023551162586262 50-51 22.02386253050692 26.694657909273484 26.792316685480365 24.48916287473923 52-53 20.477499499795567 27.90774444658618 26.25267443399064 25.36208161962761 54-55 22.309519492625824 27.768773339047335 28.603255882950844 21.318451285375996 56-57 21.97034126749565 27.200556207114353 26.484785230933777 24.344317294456218 58-59 20.513791426705946 26.379051097096294 27.37461527684042 25.73254219935734 60-61 20.84532804364224 26.277349370284863 29.776374722571653 23.100947863501244 62-63 20.330263014516483 26.187264724115416 29.566629947375123 23.91584231399298 64-65 23.175869379539996 28.06871112724772 27.618714915942483 21.1367045772698 66-67 21.86452532351948 30.143189922103492 26.038198478700085 21.954086275676943 68-69 21.229350455378576 27.662090826631964 28.411653442502704 22.696905275486753 70-71 20.93535711145951 30.890585393192577 26.72378899324532 21.450268502102595 72-73 23.365217932786393 30.329387707632982 25.971624827955033 20.3337695316256 74-75 24.764070328001825 28.19697554884656 26.457640261423425 20.58131386172819 76-77 23.386810795367268 27.12799724733656 26.4819546474432 23.003237309852974 78-79 23.328592172987562 28.7645122202206 26.308243186041437 21.598652420750394 80-81 22.563478010047515 29.24206403258059 26.387370671745746 21.80708728562615 82-83 22.057276285969373 28.793329212737223 27.01636792344848 22.133026577844927 84-85 22.724219727572535 28.34849032182446 26.544279879366762 22.383010071236242 86-87 20.30724868728986 29.09002752358895 27.990114125715703 22.612609663405486 88-89 19.78817499646302 29.334699897026965 28.44287743919944 22.434247667310572 90-91 21.20554243754982 28.626762288457847 26.891820179384723 23.275875094607606 92-93 21.705847500155677 28.68708264193379 25.887235024560713 23.719834833349818 94-95 22.30791384958017 29.737970891861426 25.759915091341828 22.194200167216575 96-97 21.23213578031809 31.23843403171196 24.464803168769098 23.064627019200852 98-99 21.379995892869367 29.089817269316736 26.37359366833386 23.156593169480036 100-101 22.804610500525524 29.829815690326235 25.918806857738353 21.44676695140989 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 265.0 1 166.0 2 88.5 3 155.0 4 186.0 5 159.5 6 146.0 7 167.0 8 181.0 9 177.0 10 189.5 11 206.5 12 207.5 13 207.0 14 239.0 15 274.5 16 280.5 17 286.5 18 332.5 19 380.0 20 398.0 21 432.5 22 487.0 23 555.0 24 666.0 25 822.0 26 1064.5 27 1401.0 28 1810.5 29 2349.0 30 3052.5 31 4032.5 32 5566.0 33 8259.5 34 13138.0 35 22776.5 36 35329.0 37 49484.5 38 66946.5 39 81144.5 40 91280.5 41 98298.0 42 131020.0 43 167706.0 44 158721.0 45 135614.0 46 130616.0 47 126666.5 48 112343.5 49 101569.5 50 92217.5 51 86923.5 52 85957.5 53 86298.0 54 73127.5 55 46598.5 56 30768.0 57 24793.0 58 21434.0 59 17894.5 60 14133.0 61 11204.5 62 8560.0 63 6459.5 64 4970.5 65 3714.0 66 2865.5 67 2267.0 68 1765.5 69 1322.0 70 941.5 71 711.5 72 500.0 73 323.5 74 216.5 75 148.5 76 112.5 77 66.5 78 40.5 79 28.5 80 20.0 81 14.5 82 11.0 83 10.5 84 8.0 85 4.0 86 2.5 87 2.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15493441384520293 2 0.0 3 0.005906187761829545 4 0.008332761028317651 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.28922006272463E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.831376050179704E-4 24-25 4.57844012544926E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.951972163084037E-4 40-41 0.0 42-43 0.0 44-45 2.2892200627246296E-4 46-47 0.001396424238262024 48-49 0.0024723576677426 50-51 6.638738181901426E-4 52-53 0.001968729253943182 54-55 0.0019229448526886892 56-57 0.0032735846896962202 58-59 0.005127852940503171 60-61 0.0012590710344985464 62-63 5.494128150539112E-4 64-65 9.385802257170982E-4 66-67 0.007439965203855046 68-69 0.0065471693793924405 70-71 0.008332761028317651 72-73 0.005860403360575053 74-75 0.00840143763019939 76-77 0.003822997504750132 78-79 0.003914566307259117 80-81 0.0012590710344985464 82-83 0.0 84-85 4.120596112904333E-4 86-87 0.001075933429480576 88-89 0.003800105304122885 90-91 0.005906187761829545 92-93 0.007966485818281712 94-95 0.007142366595700845 96-97 0.009637616464070691 98-99 0.006570061580019687 100-101 0.006203786369983747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2184150.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.575473356819266 #Duplication Level Percentage of deduplicated Percentage of total 1 86.82526935773032 36.96626942238703 2 5.727054371398188 4.876641016050377 3 2.0858315999213732 2.664158031277924 4 1.0734223680215225 1.828058617212567 5 0.7543066881073587 1.6057482151192717 6 0.5330869286744193 1.3617856997187812 7 0.37816824084725964 1.12704843038114 8 0.24949448911377034 0.8497876779149252 9 0.26564424316098073 1.017893645738348 >10 1.699749941829693 14.354753055699657 >50 0.22498314953724624 6.783497722614973 >100 0.168124506311474 13.660974055653647 >500 0.00974937432151863 2.846637056915489 >1k 0.004354804883041223 3.249423337063569 >5k 2.1712461195981829E-4 0.6781532745337572 >10k+ 5.428115298969301E-4 6.129170741718506 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 57504 2.632786209738342 TruSeq Adapter, Index 27 (97% over 39bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 30060 1.3762791017100473 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24087 1.1028088730169632 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10575 0.4841700432662592 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10380 0.4752420850216332 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 9094 0.41636334500835565 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5578 0.25538539019755974 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4970 0.22754847423482819 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4426 0.20264175995238423 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 2765 0.12659386946867202 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2621 0.1200009156880251 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2542 0.11638394798892018 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2425 0.11102717304214454 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2308 0.10567039809536892 No Hit GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC 2288 0.10475471007027905 No Hit CTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAA 2237 0.10241970560629994 No Hit AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA 2192 0.10035940754984778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0026554952727605705 0.0 0.0 2 0.003067554884051004 0.0 0.0 3 0.0034796144953414374 0.0 0.0 4 0.003571183297850422 0.0 0.0 5 0.003937458507886363 0.0 0.0 6 0.004441086921685782 0.0 0.0 7 0.004715793329212737 0.0 0.0 8 0.005173637341757663 0.0 0.0 9 0.006226678570610993 0.0 0.0 10-11 0.009408694457798229 0.0 0.0 12-13 0.012682279147494448 0.0 0.0 14-15 0.016894444062907767 0.0 4.578440125449259E-5 16-17 0.022617494219719342 0.0 4.578440125449259E-5 18-19 0.02987432181855642 0.0 4.578440125449259E-5 20-21 0.03376599592518829 0.0 4.578440125449259E-5 22-23 0.03809262184373784 0.0 4.578440125449259E-5 24-25 0.04246503216354188 0.0 4.578440125449259E-5 26-27 0.04752420850216331 0.0 4.578440125449259E-5 28-29 0.0528580912483117 0.0 4.578440125449259E-5 30-31 0.10715839113613992 0.0 4.578440125449259E-5 32-33 0.11533090676006685 0.0 4.578440125449259E-5 34-35 0.12196964494196827 0.0 4.578440125449259E-5 36-37 0.12975299315523203 0.0 4.578440125449259E-5 38-39 0.137559233569123 0.0 4.578440125449259E-5 40-41 0.1473570954375844 0.0 4.578440125449259E-5 42-43 0.1585742737449351 0.0 4.578440125449259E-5 44-45 0.17130233729368405 0.0 4.578440125449259E-5 46-47 0.18485452006501385 0.0 4.578440125449259E-5 48-49 0.199459744065197 0.0 4.578440125449259E-5 50-51 0.21772772016573955 0.0 4.578440125449259E-5 52-53 0.23807888652336148 0.0 4.578440125449259E-5 54-55 0.2628253554014147 0.0 4.578440125449259E-5 56-57 0.2893116315271387 0.0 4.578440125449259E-5 58-59 0.32069683858709336 0.0 4.578440125449259E-5 60-61 0.3548977863241993 0.0 4.578440125449259E-5 62-63 0.3940663415974177 0.0 4.578440125449259E-5 64-65 0.4362337751528054 0.0 4.578440125449259E-5 66-67 0.4824531282192157 0.0 4.578440125449259E-5 68-69 0.5335027356179749 0.0 4.578440125449259E-5 70-71 0.591969416019962 0.0 4.578440125449259E-5 72-73 0.6552892429549253 0.0 4.578440125449259E-5 74-75 0.725064670466772 0.0 6.867660188173888E-5 76-77 0.8045463910445712 0.0 9.156880250898518E-5 78-79 0.8976718631962091 0.0 9.156880250898518E-5 80-81 0.9991072041755373 0.0 9.156880250898518E-5 82-83 1.1134537463086327 0.0 9.156880250898518E-5 84-85 1.23867408373967 0.0 9.156880250898518E-5 86-87 1.3771947897351373 0.0 9.156880250898518E-5 88-89 1.530893024746469 0.0 9.156880250898518E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTCG 40 7.4829277E-6 59.461437 1 GTCGCGC 25 0.004993048 57.082977 1 CGGAAGA 10610 0.0 55.965836 4 TCGGAAG 10630 0.0 55.907784 3 GAAGAGC 10645 0.0 55.862125 6 GATCGGA 11060 0.0 53.805607 1 ATCGGAA 11095 0.0 53.607468 2 GTGCGAC 50 2.8036298E-5 47.569145 1 GGAAGAG 12795 0.0 46.66097 5 TTGTGTT 22630 0.0 45.19384 1 GCCGTCT 6910 0.0 43.030354 50-51 ATGCCGT 6920 0.0 42.86423 48-49 TGCCGTC 6910 0.0 42.652294 50-51 TATGCCG 6940 0.0 42.638042 48-49 TCTCGTA 6845 0.0 42.534935 42-43 TCGTATG 7035 0.0 42.50014 44-45 GTATGCC 7070 0.0 42.22257 46-47 CGTATGC 7005 0.0 42.13973 46-47 CTCGTAT 6830 0.0 42.072025 44-45 TGTGTTT 41290 0.0 41.900944 1 >>END_MODULE