FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l01n01_30230-1.34100000035714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l01n01_30230-1.34100000035714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184150
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT575042.632786209738342TruSeq Adapter, Index 27 (97% over 39bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA300601.3762791017100473No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC240871.1028088730169632No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC105750.4841700432662592No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT103800.4752420850216332No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT90940.41636334500835565No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC55780.25538539019755974No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT49700.22754847423482819No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT44260.20264175995238423No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC27650.12659386946867202No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT26210.1200009156880251No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC25420.11638394798892018No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC24250.11102717304214454No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA23080.10567039809536892No Hit
GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC22880.10475471007027905No Hit
CTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAA22370.10241970560629994No Hit
AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA21920.10035940754984778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG407.4829277E-659.4614371
GTCGCGC250.00499304857.0829771
CGGAAGA106100.055.9658364
TCGGAAG106300.055.9077843
GAAGAGC106450.055.8621256
GATCGGA110600.053.8056071
ATCGGAA110950.053.6074682
GTGCGAC502.8036298E-547.5691451
GGAAGAG127950.046.660975
TTGTGTT226300.045.193841
GCCGTCT69100.043.03035450-51
ATGCCGT69200.042.8642348-49
TGCCGTC69100.042.65229450-51
TATGCCG69400.042.63804248-49
TCTCGTA68450.042.53493542-43
TCGTATG70350.042.5001444-45
GTATGCC70700.042.2225746-47
CGTATGC70050.042.1397346-47
CTCGTAT68300.042.07202544-45
TGTGTTT412900.041.9009441
Produced by FastQC (version 0.11.2)