FastQCFastQC Report
Fri 7 Aug 2015
C6001ACXX l08n02.3320000000d389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l08n02.3320000000d389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157617231
Sequences flagged as poor quality0
Sequence length7
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCT5423299034.408033725703504No Hit
AGGTCGA5394197734.22340099351193No Hit
GGTCGAC89859895.701146342305684No Hit
CGAGCTC87520025.552693664565139No Hit
TAGAGCT24179021.5340340549441576No Hit
GAGCTCG14720220.933922002474463No Hit
GTCGACA14590260.9256767110697435No Hit
TCGAGAT12776910.8106290104791907No Hit
AGGTAGA8096730.5136957392685068No Hit
AGGACGA7045810.44702028802929544No Hit
CAGGTCG6203770.3935971949665833No Hit
TAGAGAT5708150.3621526633721918No Hit
AGCTCGT5624920.356872149340068No Hit
AGAGCTC4724930.29977242780010516No Hit
CGGTCGA4432810.2812389211430824No Hit
TCGCGCT4315370.2737879591350009No Hit
GGTCGAA4223800.26797831513738496No Hit
AGGTCGC4116650.2611802005327704No Hit
TGGAGCT3642090.23107181726850665No Hit
TCGAGCA3543170.2247958536969857No Hit
TCAAGCT3335700.21163295274486835No Hit
TCGACAT2878600.1826323163867788No Hit
GCTCGTA2787540.17685502925755622No Hit
AGAGCTA2787020.17682203794076298No Hit
TTCGAGC2672530.16955823821064334No Hit
CGAGCTA2495250.15831073697773562No Hit
ACGAGCT2405980.15264701611208992No Hit
CTCGTAT2355060.14941640485994834No Hit
GGTCGCC2242270.14226046135780676No Hit
AGTCGAC1812830.11501470927375954No Hit
TAGAGCA1745270.11072837588423311No Hit
AGGTCAA1713070.10868545203664946No Hit
CGAGATC1711030.10855602456307585No Hit
AGGGCGA1644570.1043394804975352No Hit
ACGAGCA1598440.10141277002893166No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)