FastQCFastQC Report
Fri 7 Aug 2015
C6001ACXX l08n01.3310000000d38c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l08n01.3310000000d38c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157617231
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGT84145000.044.914287
CGGCGTG1488450.044.906512
GTGTCGG84164600.044.905966
TCGGTCT84143700.044.8902449
ACGCGTG1663650.044.8902052
GTCGGTC84207850.044.877748
CTTCGTG1347200.044.877122
CGGTCTC84190700.044.86673410
TGCGGGT2395850.044.8476451
GGGTGTC3898750.044.829674
ACCGGTG1248600.044.8281172
TAGTACT414650.044.81396518
GTAGGGG590450.044.8095117
CTCGTAG82715600.044.8081214
GGTCTCG84310650.044.80591211
TTCGTGT1350100.044.8055343
GGTCTCT72709650.044.8034214
GTCTCGT84214300.044.8003812
ACGCGTC1337250.044.798852
ACGAGGT73113900.044.7873110