FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l07n01 tcfgrna-18h-2.3410000000d751.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l07n01 tcfgrna-18h-2.3410000000d751.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460636
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATGCCCTTAGATGTTCGGGGCCGCACGCGCGCTACACTGACCGGATCA161200.6551151816034554No Hit
CCCGACGCGACGCTCATGAGAGCCCACAAAAGGTGTTGGTTGATATAGACA129450.5260835003633206No Hit
TGATTGGAGCCGGGCGTGGAATGCGAGTGCCAAGTGGGCCACTCTTGGTAA107250.4358629232442344No Hit
TAGTCCCTCTAAGAAGTTAAACGCCGACCGGGAGGGTCGCGTAACTATTTA79590.32345296094180526No Hit
GAAACGCCCTCAACCTATTCTCAAACTTTAAATTGGTAAGAAGCCCGACTC79460.3229246422469638No Hit
AGGGCCTGACACCCGCTAGGGGACGAAGCCTCGATCAGAAGAACGTGGGCA78270.31808849419418395No Hit
GTCGGTTTTTGGTCTGATAAAAGCACGCCTCCCGCGAAGTCGGCGCTGTTT71760.29163191955250595No Hit
GGGTGAGATTTCCCGTGTTGAGAGCTCTTTCTTGATTCTGTGGGTGGTGGT54140.2200244164516816No Hit
ACAGACCGCGAAAGCGTGGCCTATCGATCCTTTTGAACTTGCGAGTTTCAA46500.1889755331548429No Hit
AGGTTCGGGAATATTAACCCGATTCCCTTTCGGTCCGCCGGGGCAACGCGA44210.1796689961457119No Hit
TAGTCCCTCTAAGAAGTTAGACGCCGACCGGGAGGGTCGCGTAACTATTTA43080.17507668749055122No Hit
CGAAGCGGGGTGCGGCGCTGTACCGGACTGGGCGAACGCCTTCGGGTGTGA42110.1711346172290416No Hit
AGGACACCTGCGTTACTCTTTGACAGATGTACCGCCCCAGTCAAACTCCCC39760.16158424082229148No Hit
CCCGGACCTTTCGGCCAAGGAAGAGACACGCTGATCCGGTCAGTGTAGCGC33180.13484318688339111No Hit
AACCTATTCTCAAACTTTAAATTGGTAAGAAGCCCGACTCGCTTGATTGGA33040.13427422828894642No Hit
AAGTTTCCCTCAGGATAGCTGGCGCTCTGTCGCAGTTTTATCTGGTAAAGC30100.12232609780560798No Hit
GAAACCTCGCGTAGAGCAAAAGGGCAAAAGCTCACTTGATTTTGATTTTCA28230.11472643657981108No Hit
CTTGGGGGCGAAACGCCCTCAACCTATTCTCAAACTTTAAATTGGTAAGAA28000.11379171888893766No Hit
ACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC27970.11366979919012808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA18600.039.43334631
CCCGCGA8000.039.37286431
GCGCTAC18900.038.92646432
GCCTCCC8050.038.2898527
TCCCGCG8300.037.9497530
CACGCGC19300.037.76997427
TCCGCCG6250.037.4379734
CGCACGC19500.037.15183325
GCACGCC8300.037.13654323
GGTCCGC6400.036.91206432
GCTACAC20000.036.78550334
GCACGCG19700.036.7746626
CGCTACA20050.036.6937733
CCGCACG19850.036.61010724
TGACCGG20100.036.60249341
GACCGGA20350.036.48747642
CTACACT20250.036.22025735
CACGCCT8450.036.21105624
CGCCTCC8550.035.78753726
CCGGATC20800.035.70533844
Produced by FastQC (version 0.11.2)