FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l07n01 tcfgrna-18h-1.3410000000d5d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l07n01 tcfgrna-18h-1.3410000000d5d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906876
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGTAGCTGGTTCCCTTCGAAGTTTCCCTCAGGATAGCTGGCGCTCTG127401.4048227100507678No Hit
CGGCCCGAAGACCTCCCACTTATCCTACACCTCTCATGTCTCTTCACAAAG99861.101142824377313No Hit
GGGAAGAGCCCATCGCCGAATCCGCGCGTTCTTGGAGCGCGCGGAATTGTG91461.0085171511871522No Hit
CCGAAGGTCGCTCTCCGCTTTACTGAATAAGTAAAAAAACGATGCGAGTAG71150.7845615056523715No Hit
CAGCGTGTCTCTTCCTTGGCCGAAAGGTCCGGGAAACCCGTTGAACCCCGG69340.7646028784530631No Hit
AAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTG60350.6654713544078794No Hit
CCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCTATACCCGGCCGTCGCGG52010.5735072931690771No Hit
CCCGTGGAATGCCGCAGCTAATGCGGGCTTCGCGGCCCGCTTCGGCCGGCG51120.5636933825572625No Hit
CCGTCGCCGGTGCTAGACCGTGCGATCCGCCAGGTTATCCAGAGTCACCAG48090.5302819790136689No Hit
AGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTG47130.5196961877919363No Hit
CTCCCCGCGTCGGGAATGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG44460.4902544559564924No Hit
TCTCGGCGTCTTCCCGGAGTCGGGTTGTTTGGGAATGCAGCCCAAAGCGGG41380.4562917091201002No Hit
AACTTTCCGGCCGCCGCCGACACTCAGTCAAGAGCACCGCCGGAGAACCGG35100.38704299154459926No Hit
CGAAGCCTAGGGCGCGAGCCCGGGTGGAGCAGCCGTCGGTGCAGATCTTGG30050.3313573189719432No Hit
CCCTCTTAATCATTACCTCGCGCTCCGAAAACCAACAAAATAGAACCGAGG29300.32308716957996464No Hit
CCGCGTCGGCCCGTGAAAATCCGAGGGAGATGGTGTAATTTTCGTGCGAGG28620.315588900797904No Hit
AGCCTAGGGCGCGAGCCCGGGTGGAGCAGCCGTCGGTGCAGATCTTGGTGG25540.2816261539615118No Hit
CCGAGGTGGTGCGATAGACCGCGACGAGTAGGAGGGCCGCGGCGGTGGGCG21940.24192943688001448No Hit
CTGACTTTCGCTCTTGATTAAAGAAAACATTCTTGGCAAATGCTTTCGCAG21440.23641600395202875No Hit
GCCCACAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAG19570.21579576480136203No Hit
TGCGTCAACACCAGGTGACGGCCATCGCAAAGCTTTGTTTTAATTAAACAG18900.20840776467786112No Hit
GCGTGCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATC18240.20113003321291997No Hit
CACGAACGCATCGTCGCTTTCCAGGCGGGATTCTGACTTAGAGGCGTTCAG17740.1956166002849342No Hit
TGAACCAAATGTCTGAATCTGCGGTTCCTCTCGTACTGAGCAGGATTACTG13770.15183994283672742No Hit
TTGGCTTATCAGCCAAGCACATGAACCAAATGTCTGAATCTGCGGTTCCTC10870.11986203185441009No Hit
TAACTTTGTGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTG10040.11070973319395375No Hit
TGCCGACCGCGCCGGGTCCAATCGGAGGACCTCACTAAACCATTCAATCGG9780.10784274807140116No Hit
CGACGCTCCAGCGCCATCCATTTTCAGGGCTGATTGATTCGGCAGGTGAGT9450.10420388233893056No Hit
TACTTCCCCCGGAACCCAAAGACTTTGGTTTCCCGCAAGCTGCCGGGGAGG9350.10310119575333342No Hit
TTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAG9240.10188824050917655No Hit
TGTTGGTTTTCGGAGCGCGAGGTAATGATTAAGAGGGACAGACGGGGGCAT9140.10078555392357941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGAC5050.041.4338712
ATCCGCG10500.041.3599320
TCGCGGC6850.041.05663330
TGCGGGC6800.041.03217722
TGCTAGA5100.041.02765711
GCGCGTT10550.040.94611424
GGCCGGC7000.040.8196844
GGCCCGC6850.040.73042734
AGGTCCG7850.040.6988925
GCTGCCT5750.040.69614835
GCGGGCT6900.040.4330523
TCCGCGC11050.040.3194621
CCGTGCG5200.040.24309518
CTAGACC5200.040.23865513
AACCCGT8000.040.21924235
CCGCGCG11050.040.11582622
GCCGCAG6900.040.10697611
GGATAGC16350.040.0463635
CCTACAC10800.039.99828724
GGTCCGG8000.039.93578726
Produced by FastQC (version 0.11.2)