C6001ACXX l07n01 fzgrna-18h-2.3410000000d7d7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	C6001ACXX l07n01 fzgrna-18h-2.3410000000d7d7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1596461
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTATCACGACCGGGGTTCAACGGGTTTCCCGGACCTTTCGGCCAAGGAAG	26978	1.689862765203785	No Hit
CTGAGCTCACCTTAGGACACCTGCGTTACTCTTTGACAGATGTACCGCCCC	24515	1.5355840199040252	No Hit
GCGCGTCCTGCTGCCCACGTTCTTCTGATCGAGGCTTCGTCCCCTAGCGGG	21786	1.3646434206660858	No Hit
CTAGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCG	21401	1.3405275794397733	No Hit
GACCTCCCACTTATCCTACACCTCTCATGTCTCTTCACAAAGTCGGACTAG	14778	0.9256724717985594	No Hit
CAAGAGTGGCCCACTTGGCACTCGCATTCCACGCCCGGCTCCAATCAAGCG	13673	0.8564568755516108	No Hit
GGGTGTCAGGCCCTTGGCGGCGGCGGGTGCGCGCTCTCGGCGTCTTCCCGG	8453	0.5294836516519977	No Hit
TTGAATGATCCGTCGCCGGTGCTAGACCGTGCGATCCGCCAGGTTATCCAG	7654	0.4794354512888194	No Hit
CCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACAG	6457	0.4044571085670116	No Hit
TGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAG	5489	0.3438229934837118	No Hit
TGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCG	5481	0.3433218850945936	No Hit
TACAGCGCCGCACCCCGCTTCGTGCCACGGCCCGACCGACCCAGCCCCTAG	5134	0.32158630871659255	No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAG	4131	0.25875984443090055	No Hit
GTCCTGCTGTCTATATCAACCAACACCTTTTGTGGGCTCTCATGAGCGTCG	4000	0.2505541945590904	No Hit
CGGGGAGGTCGTCAAATTAACGCTCCCCGATCGCTAGTTGGCATCGTTTAT	3555	0.2226800404143916	No Hit
AAGCTTTGTTTTAATTAAACAGTCGGATTCCCCCGGTCCGTACCAGTTCTA	3201	0.20050599419591209	No Hit
TTGAACACTCTAATTTTTTCAAAGTAAACTTTCCGGCCGCCGCCGACACTC	3145	0.1969982354720848	No Hit
AATTGGTAAGAAGCCCGACTCGCTTGATTGGAGCCGGGCGTGGAATGCGAG	3081	0.19298936835913938	No Hit
ATTCGATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATC	3019	0.18910577834347347	No Hit
TAAGAAGTTAAACGCCGACCGGGAGGGTCGCGTAACTATTTAGCAAGCCAG	2814	0.1762648758723201	No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTAAACGCCACT	2405	0.1506457094786531	No Hit
AACTTTCGTCGGTACGGTATTCGCCTACCGAGGTTCTTACGGGTAACGGGG	2007	0.1257155671200236	No Hit
GCGTTCTTGGAGCGCGCGGAATTGTGGCGTACGGAATTCGCTCTGCGCGCG	1978	0.12389904920947019	No Hit
ACCACCACCCACAGAATCAAGAAAGAGCTCTCAATCTGTCAATCCTCACTG	1905	0.11932643515876679	No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCG	1857	0.1163197848240577	No Hit
CATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAG	1774	0.11112078528695658	No Hit
GGTTGAATTTCTTCACTTTGACATTCAGAGCACTGGGCAGAAATCACATTG	1772	0.11099550818967703	No Hit
TAAATTGGTAAGAAGCCCGACTCGCTTGATTGGAGCCGGGCGTGGAATGCG	1718	0.10761302656312932	No Hit
TCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGG	1635	0.1024140270260282	No Hit
TGGGCTCTACCGACGACCCTTCGGGCCGACTAACTATGCCTCTCGAGGCCG	1613	0.1010359789559532	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCCACG	2385	43.958256	13
GCTGCCC	2425	43.325943	10
CGCGTCC	2465	42.993355	2
CCCACGT	2455	42.97979	14
GCGTCCT	2465	42.896698	3
TCCCCTA	2445	42.882214	40
GGCCCAC	1560	42.83263	8
TGCCCAC	2465	42.62289	12
CGTCCCC	2485	42.46491	38
CCACGTT	2480	42.457134	15
CCCTAGC	2470	42.44818	42
CCTAGCG	2485	42.373035	43
CTAGCGG	2490	42.3012	44
GTCCCCT	2500	42.210117	39
GCCCACT	1590	42.165966	9
ACGTTCT	2505	42.12982	17
CCCGGCT	1595	42.037735	34
CGCATTC	1595	42.03642	23
CACGCCC	1590	42.024467	30
TCGTCCC	2525	41.970413	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content