FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l07n01 fzgrna-18h-1.3410000000d638.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l07n01 fzgrna-18h-1.3410000000d638.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6358660
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATCACGACCGGGGTTCAACGGGTTTCCCGGACCTTTCGGCCAAGGA297940.4685578408029365No Hit
CAGACCTGTTATTGCTCAATCTCGTGTGGCTAAACGCCACTAGTCCCTCTA204860.3221747978347639No Hit
GAACCGAACGCGGGGTTAAGGCGCCCGACGCGACGCTCATGAGAGCCCACA176940.2782661755778733No Hit
TCTGGCTTGCTAAATAGTTACGCGACCCTCCCGGTCGGCGTTTAACTTCTT171580.2698367266059201No Hit
GTAAGAAGCCCGACTCGCTTGATTGGAGCCGGGCGTGGAATGCGAGTGCCA164770.25912692296804674No Hit
AGGTCTTGGGGGCGAAACGCCCTCAACCTATTCTCAAACTTTAAATTGGTA148920.23420028748195373No Hit
GCGCCTTAACCCCGCGTTCGGTTCATCCCGCATCGCCAGTCCTGCTTACCA144490.22723341081297005No Hit
GGATTCCCCCGGTCCGTACCAGTTCTAAGTCAGCTGTTCGACGCCGGCCGA118520.1863914724171445No Hit
GCCCGGCTCCAATCAAGCGAGTCGGGCTTCTTACCAATTTAAAGTTTGAGA110120.17318114193871037No Hit
GGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAACAACCCGACTCCGGGA106160.16695341471316283No Hit
GCCGGCCGAAGCGGGCCGCGAAGCCCGCATTAGCTGCGGCATTCCACGGGA105390.1657424677526397No Hit
CTCAGGATAGCTGGCGCTCTGTCGCAGTTTTATCTGGTAAAGCGAATGATT105260.16553802216190203No Hit
TCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAA103430.16266005730767175No Hit
GGCCGTCGGTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTAA97070.15265794994542875No Hit
TCTGGCTTGCTAAATAGTTACGCGACCCTCCCGGTCGGCGTCTAACTTCTT96490.15174580807906068No Hit
TATCCTACACCTCTCATGTCTCTTCACAAAGTCGGACTAGAGTCAAGCTCA93920.14770407601601596No Hit
TATCCCTGTGGTAACTTTTCTGGCACCTCTTGCTTGAAACTCGCAAGTTCA81840.12870636266131544No Hit
AAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAGAGACATG78990.12422428624898957No Hit
TCCCTATCTCCGGGCAAACCGATTCCAGGGTCGCCACCCCTTACCAAGAAA74310.11686424498243342No Hit
TTAACTTCTTAGAGGGACTAGTGGCGTTTAGCCACACGAGATTGAGCAATA74180.11665979939169573No Hit
CTTTGTGTTTGACGAGGCTACCCGCTGAATTTAAGCATATTACTAAGCGGA72800.11448953081309585No Hit
GAACTCGGAAACGTTGGCGTGGGTCCCGGGAAGAGTTCTCTTTTCTTGGTA67890.10676777811677303No Hit
TAACTTTGTGTTTGACGAGGCTACCCGCTGAATTTAAGCATATTACTAAGC67510.10617016792846291No Hit
TTGATTGGAGCCGGGCGTGGAATGCGAGTGCCAAGTGGGCCACTCTTGGTA66610.10475477537720211No Hit
GGACCTTTCGGCCAAGGAAGAGACACGCTGATCCGGTCAGTGTAGCGCGCG66540.10464468928988184No Hit
TGAGCTTGACTCTAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGGATA65260.10263168655031091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTTA18750.036.96012
CGCCGGC23350.035.9434142
GCGCCTT19400.035.7425421
CCCCGCG18200.035.72502510
CATCCCG21050.034.41597724
CAGTCCT20600.034.29431537
CGACGCC24750.034.18002739
CGCCAGT20900.034.12500834
CCGCATC21450.033.983728
GCATCGC21150.033.82748430
CCCTCCC41450.033.7615326
CGTTCGG21050.033.66806815
GCCCGGC15250.033.5113951
GCCAGTC21450.033.35489735
ACCCTCC41850.033.3313225
ACCCCGC19600.033.2880259
CCCGCGT19550.033.25808311
CTCCGCG4550.033.1319582
TCATCCC21800.033.0255323
GCTTACC22100.032.99429344
Produced by FastQC (version 0.11.2)