FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l07n01 controlgrna-18h-2.3410000000d6d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l07n01 controlgrna-18h-2.3410000000d6d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4594127
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGG114600.24944891597467814No Hit
GTCGGTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCG103980.22633244575084666No Hit
CCCGCCGCCGCCAAGGGCCTGACACCCGCTAGGGGACGAAGCCTCGATCAG91810.19984210275423384No Hit
CCACGCCCGGCTCCAATCAAGCGAGTCGGGCTTCTTACCAATTTAAAGTTT72190.15713540352715544No Hit
ATCAGACCAAAAACCGACCGCGGCTCCGGCCGCGTTCGACTGGTGACTCTG68210.14847216892349732No Hit
GGCCCGCTTCGGCCGGCGTCGAACAGCTGACTTAGAACTGGTACGGACCGG67670.1472967551833025No Hit
CCCTATCTCCGGGCAAACCGATTCCAGGGTCGCCACCCCTTACCAAGAAAA66750.1452941984407484No Hit
GTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCACTGGG62910.13693570073269634No Hit
CCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTAAGAACCTCGGTAG58900.12820716536569407No Hit
GCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTG53740.11697543407049914No Hit
GGGTCGGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCGGACTGG53740.11697543407049914No Hit
AGAGTTCTCTTTTCTTGGTAAGGGGTGGCGACCCTGGAATCGGTTTGCCCG53280.11597415569922208No Hit
GCCCCGGCGGACCGAAAGGGAATCGGGTTAATATTCCCGAACCTCGACACG52210.11364509513994715No Hit
TCAACCTATTCTCAAACTTTAAATTGGTAAGAAGCCCGACTCGCTTGATTG51600.11231731295194931No Hit
GCGCTCTGTCGCAGTTTTATCTGGTAAAGCGAATGATTAGAGGTCTTGGGG50730.11042359081496878No Hit
ACGTCTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGG50000.10883460557359428No Hit
CTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAG47870.10419825137615918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCGC13800.040.301991
GCCCGCT14150.039.593622
GGCCGGC15000.037.34713711
ACGCCCG10250.037.3142133
CGCCCGG10400.036.7760244
CCCGGCT10600.036.2943846
CGCTTCG15450.036.2593575
CACGCCC10500.036.2142682
CCGGCGT15800.035.17135613
GCCCGGC11150.034.705865
CCGCTTC16250.034.6127324
CCGCCAA12300.034.570528
TCGGCCG16300.034.368539
GCGGCTC10300.034.08284820
GCCGCCA12300.034.0217827
GCCGCGT10500.033.42637329
CCGGCTC11550.033.309137
CCCCTTA8500.033.0864236
CCCGCCG12800.033.0716971
ACCCGCG9300.032.9007035
Produced by FastQC (version 0.11.2)