FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l07n01 controlgrna-18h-1.3410000000d57c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l07n01 controlgrna-18h-1.3410000000d57c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952106
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGACACGGTCTTCAGATCGAATCGCGCCGCACCCGAAAGCGCGCGCTTA266821.3668315142722782No Hit
CGAGAGCGCGCACCCGCCGCCGCCAAGGGCCTGACACCCGCTAGGGGACGA151490.7760336784990159No Hit
TCTTGGGGGCGAAACGCCCTCAACCTATTCTCAAACTTTAAATTGGTAAGA127090.6510404660402662No Hit
GGTCGGGCCGTGGCACGAAGCGGGGTGCGGCGCTGTACCGGACTGGGCGAA85630.4386544583132268No Hit
GTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCT85480.43788605741696407No Hit
TCCCTCTAAGAAGTTAAACGCCGACCGGGAGGGTCGCGTAACTATTTAGCA57490.2945024501743246No Hit
TCCGTACCAGTTCTAAGTCAGCTGTTCGACGCCGGCCGAAGCGGGCCGCGA53910.27616328211685226No Hit
TGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA50460.25849006150280773No Hit
CGCCGCACCCCGCTTCGTGCCACGGCCCGACCGACCCAGCCCCTAGAGCCA48970.25085727926659723No Hit
TCGGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTAATTCTGCCA45950.23538680788850608No Hit
ACGAAGCGGGGTGCGGCGCTGTACCGGACTGGGCGAACGCCTTCGGGTGTG45860.23492576735074838No Hit
AACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTAAA43290.22176049866144565No Hit
TAGGACACCTGCGTTACTCTTTGACAGATGTACCGCCCCAGTCAAACTCCC38230.19583977509418032No Hit
ACCAGTCGAACGCGGCCGGAGCCGCGGTCGGTTTTTGGTCTGATAAAAGCA34740.17796164757446573No Hit
AGCGCCATCCATTTTCAGGGCTGATTGATTCGGCAGGTGAGTTGTTACACA33630.17227548094212097No Hit
TGTCTATATCAACCAACACCTTTTGTGGGCTCTCATGAGCGTCGCGTCGGG33200.1700727317061676No Hit
CGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACA30380.15562679485642683No Hit
TCCCTCTAAGAAGTTAGACGCCGACCGGGAGGGTCGCGTAACTATTTAGCA29190.14953081441274194No Hit
ATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGA24430.12514689263800224No Hit
AATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATACTCGTTAA23630.1210487545246006No Hit
ACGGCCATGCACCACCACCCACAGAATCAAGAAAGAGCTCTCAATCTGTCA23500.12038280708117285No Hit
CGCCTATTCCTTAGGGTCGACTGACCCATGTTCAACTGCTGTTCACATGGA22870.11715552331686907No Hit
GGTGGTGCGATAGACCGCGACGAGTAGGAGGGCCGCGGCGGTGGGCGTCGA22310.11428682663748792No Hit
AATTTTCGTGCGAGGTCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAAC22140.11341597228839008No Hit
CGGTCTTCAGATCGAATCGCGCCGCACCCGAAAGCGCGCGCTTAAGGCTAG21150.10834452637305556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCC17900.041.3510512
GGGCCTG18100.041.2680927
GCCGCCG18250.040.81293516
GGCCTGA18300.040.69412628
ACACGGT31200.040.236795
CGCGCAC18300.040.201327
GCGCACC18450.040.1183628
CCGCCAA18600.040.04700520
GCCGCCA18850.039.51384719
CGCTAGG18850.039.5087939
CAAGGGC18800.039.49317624
TGACACG32000.039.230873
CCGCTAG18950.039.18156438
CGCGCCG31850.039.13508625
CACGGCC6500.039.1219121
ATCGCGC32000.039.0219523
GACACCC19150.039.00634833
GCTAGGG19100.038.99065840
CCCGCCG19000.038.95704713
CGCCAAG19050.038.8647521
Produced by FastQC (version 0.11.2)