FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l06n01 tcfgrna-15h-2.3410000000d6e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l06n01 tcfgrna-15h-2.3410000000d6e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238519
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGCTTTTGCCCTTTTGCTCTACGCGAGGTTTCCGTCCTCACTGAGCTCA232401.876434677223361No Hit
TCAAGTGAGCTTTTGCCCTTTTGCTCTACGCGAGGTTTCCGTCCTCACTGA164221.3259384797487968No Hit
GGCCATGCACCACCACCCACAGAATCAAGAAAGAGCTCTCAATCTGTCAAT108700.8776611420575703No Hit
GCGAGTAGTGGTATTTCACCGACGGCCCGAAGACCTCCCACTTATCCTACA102810.8301043423637424No Hit
CTTCGACGCCCACCGCCGCGGCCCTCCTACTCGTCGCGGTCTATCGCACCA97110.7840816329826188No Hit
AAATCAAAATCAAGTGAGCTTTTGCCCTTTTGCTCTACGCGAGGTTTCCGT78640.6349519062686967No Hit
AGAGAACTCTTCCCGGGACCCACGCCAACGTTTCCGAGTTCGCTTGCGTTA71940.5808550373470249No Hit
CCACTTATCCTACACCTCTCATGTCTCTTCACAAAGTCGGACTAGAGTCAA68340.5517880630010521No Hit
TGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCA59400.47960507670855274No Hit
CTTGGCAAATGCTTTCGCAGTCGTTCGTCTTGCGCCGATCCAAGAATTTCA59350.4792013687315253No Hit
GGCCATGCACCACCACCCACAGAATCAAGAAAGAGCTCTCAACACGGGAAA55360.4469854721647387No Hit
CTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTAAGAA52090.42058297046714666No Hit
TTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGA51700.4174340482463329No Hit
TCTTACCAATTTAAAGTTTGAGAATAGGTTGAGGGCGTTTCGCCCCCAAGA47990.38747891635089976No Hit
CACCTCTAACGGCACAGTACGAATGCCCCCGTCTGTCCCTCTTAATCATTA42710.34484735397680616No Hit
TGCTCGCGTTGCCCCGGCGGACCGAAAGGGAATCGGGTTAATATTCCCGAA38550.3112588502881264No Hit
TCACCTTAGGACACCTGCGTTACTCTTTGACAGATGTACCGCCCCAGTCAA38250.308836602425962No Hit
GTCCTCACTGAGCTCACCTTAGGACACCTGCGTTACTCTTTGACAGATGTA32580.2630561178310547No Hit
AATAAGTAAAAAAACGATGCGAGTAGTGGTATTTCACCGACGGCCCGAAGA31520.25449750871807375No Hit
CTCGCGACGGACCGACCGCCCGCGCCCAAAATCCAACTACGAGCTTTTCAA30200.24383961812455035No Hit
TGAGCTTTTGCCCTTTTGCTCTACACGAGGTTTCCGTCCTCACTGAGCTCA28620.23108244605048447No Hit
GGGATGAACCGAACGCGGGGTTAAGGCGCCCGACGCGACGCTCATGAGAGC27100.2188097235488515No Hit
GGGGCGAAAGACTAATCGAACCGTCTAGTAGCTGGTTCCCTTCGAAGTTTC26540.2142881942061446No Hit
CGCAGTTTTATCTGGTAAAGCGAATGATTAGAGGTCTTGGGGGCGAAACGC25960.20960518167262676No Hit
CATCGTCGCTTTCCAGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATC23630.19079238994314984No Hit
CGCCATCCATTTTCAGGGCTGATTGATTCGGCAGGTGAGTTGTTACACACT23130.18675531017287583No Hit
ACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACA21980.1774700267012456No Hit
ACAATTCCGCGCGCTCCAAGAACGCGCGGATTCGGCGATGGGCTCTTCCCA21010.169638091946914No Hit
TAAGGTGAGCTCAGTGAGGACGGAAACCTCGCGTAGAGCAAAAGGGCAAAA20890.16866919280204826No Hit
CACGTCAGAATCGCTACGGTCCTCCACCAGAGTTTCCTCTGGCTTCAACCT20300.16390543867312493No Hit
GCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTAAACGCCACTAGTC20280.16374395548231396No Hit
GCGCGTGCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAA19590.15817278539933582No Hit
TTGCACGTCAGAATCGCTACGGTCCTCCACCAGAGTTTCCTCTGGCTTCAA18790.1517134577668974No Hit
TCTGAATCTGCGGTTCCTCTCGTACTGAGCAGGATTACTGTCGCAACAACA18330.1479993443782453No Hit
ATCGATCCTTTTGAACTTGCGAGTTTCAAGCAAGAGGTGCCAGAAAAGTTA18310.14783786118743436No Hit
GCACGCCTCCCGCGAAGTCGGCGCTGTTTGCATGTATTAGCTCTAGAATTA18010.14541561332526992No Hit
CGCGATTCGATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTA17870.14428523098959323No Hit
CATGAATGGATTAACGAGATTCCCGCTGTCCCTATCTACTATCTAGCGAAA17840.1440430062033768No Hit
CAAAGCAGGCTCTCGAGCCTGAATAATGGTGCATGGAATAATGGAATAGGA17730.1431548486539165No Hit
CTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCAGTGAGGACGGAAA17550.14170149993661785No Hit
CAAGGAAGAGACACGCTGATCCGGTCAGTGTAGCGCGCGTGCGGCCCCGAA17280.13952147686066987No Hit
CGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGATCGATTTGCA16990.13717997059391096No Hit
TCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTA16670.13459623954093558No Hit
GACTGGCGATGCGGGATGAACCGAACGCGGGGTTAAGGCGCCCGACGCGAC16560.1337080819914753No Hit
AAGCTCAACAGGGTCTTCTTTCCCGCTGTCCCTATCTACTATCTAGCGAAA15390.12426131532903412No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCA15270.12329241618416835No Hit
GTACCAGTTCTAAGTCAGCTGTTCGACGCCGGCCGAAGCGGGCCGCGAAGC14440.11659086376551349No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCTTGCTAAATAGTTACGCGAC14170.11441084068956552No Hit
TATCGGTCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGA12740.10286479254658185No Hit
GCGACCCTCCCGGTCGGCGTTTAACTTCTTAGAGGGACTAGTGGCGTTTAG12590.10165366861549965No Hit
GGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGT12500.10092699425685032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGC8600.042.91837719
GACCCAC9950.042.74486517
CCGGGAC10050.042.30929613
CGCCGCG13250.042.1090914
CGGGACC10200.041.90766514
CGGCCCT11550.041.69938719
ACCCACG10200.041.69719718
GACGCCC13550.041.6748925
CCCACCG13500.041.662599
GGCCCTC11650.041.53463720
CACTACG3200.041.48534424
CCTACTC13750.041.39595426
CTCCTAC11800.041.37808224
CGCCCGC4250.041.30008317
CTATCGC13650.041.2097541
ACGCCCA13900.040.7873766
CTCGTCG13900.040.78737630
ACTCGTC13900.040.78737629
GGACCCA10500.040.7168816
AGTTCGC10500.040.7119537
Produced by FastQC (version 0.11.2)