FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l06n01 tcfgrna-15h-1.3410000000d5e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l06n01 tcfgrna-15h-1.3410000000d5e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897714
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTGATTGGAGCCGGGCGTGGAATGCGAGTGCCAAGTGGGCCACTCTT187742.0913119323080624No Hit
ATAGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTGATTGATTCGGCA118611.3212448508099461No Hit
TCCCTTCGAAGTTTCCCTCAGGATAGCTGGCGCTCTGTCGCAGTTTTATCT117891.3132244790657157No Hit
TTAGAGGTCTTGGGGGCGAAACGCCCTCAACCTATTCTCAAACTTTAAATT103721.1553791073771824No Hit
TGAGAGCTCTTTCTTGATTCTGTGGGTGGTGGTGCATGGCCGTTCTTAGTT101391.129424293260437No Hit
AGCGTGTCTCTTCCTTGGCCGAAAGGTCCGGGAAACCCGTTGAACCCCGGT100351.1178393118521044No Hit
TTCTGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCT73850.8226450740436264No Hit
GCTCTCAATCTGTCAATCCTCACTGTGTCCGGGCCGGGTGAGATTTCCCGT62130.6920912450958769No Hit
GCCGGGCGTGGAATGCGAGTGCCAAGTGGGCCACTCTTGGTAAGCAGGACT60360.6723744978913107No Hit
GGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT59850.6666934012391474No Hit
CAGAATCAAGAAAGAGCTCTCAATCTGTCAATCCTCACTGTGTCCGGGCCG59240.6598983640669522No Hit
GCGTGCTTTTATCAGACCAAAAACCGACCGCGGCTCCGGCCGCGTTCGACT57760.6434120443704788No Hit
GTCGAAGCCTAGGGCGCGAGCCCGGGTGGAGCAGCCGTCGGTGCAGATCTT52190.5813655574046968No Hit
GTTCATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCAT48250.5374763009154363No Hit
AAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGT46960.5231064682070236No Hit
TCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGAGGGAGAT44770.49871117081832295No Hit
AAGCGAACTCGGAAACGTTGGCGTGGGTCCCGGGAAGAGTTCTCTTTTCTT37930.4225176392481347No Hit
AACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTG33230.3701624348066311No Hit
GGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAA32520.36225345711440393No Hit
TGCCGACTTCCCTTACCTACATTGTTCTATCGGTCAGAGGCTGTTCACCTT30830.34342786232586325No Hit
CGGGCCGGGTGAGATTTCCCGTGTTGAGAGCTCTTTCTTGATTCTGTGGGT30300.33752397756969366No Hit
CAGAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTT27740.30900710025687467No Hit
GGAAACGTTGGCGTGGGTCCCGGGAAGAGTTCTCTTTTCTTGGTAAGGGGT26580.2960853902245036No Hit
CGTGCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCT25060.2791534943200173No Hit
ATGGTGTAATTTTCGTGCGAGGTCGTACCCATATCCGCAGCAGGTCTCCAA23370.2603278995314766No Hit
GCCGGGTGAGATTTCCCGTGTTGAGAGCTCTTTCTTGATTCTGTGGGTGGT21710.24183648689894555No Hit
CTCGGCGTCTTCCCGGAGTCGGGTTGTTTGGGAATGCAGCCCAAAGCGGGT21340.23771490697482717No Hit
AGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCG20490.22824641255455524No Hit
CGCGCTGTCGGCGAGGTCGAGCGAGAGCGTGCAAGTTGGGACCCGAAAGAT18590.20708154267394738No Hit
GGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT18280.2036283270618482No Hit
CGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATT17280.19248892186152827No Hit
CGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGCGGGGAGTTTGACT16790.1870306133133715No Hit
GGGGGAATCCGACTGTTTAATTAAAACAAAGCTTTGCGATGGCCGTCACCT16100.17934442372515078No Hit
GCGGACCGAAAGGGAATCGGGTTAATATTCCCGAACCTCGACACGGAGATT15100.16820501852483086No Hit
ACCACAGCCAAGGGAACGGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCT15030.16742526016080844No Hit
CTCAATCTGTCAATCCTCACTGTGTCCGGGCCGGGTGAGATTTCCCGTGTT15000.16709107800479886No Hit
CGGACACAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTGTGGGT14930.16631131964077644No Hit
GGTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT14760.1644176207567221No Hit
CTCAACCTATTCTCAAACTTTAAATTGGTAAGAAGCCCGACTCGCTTGATT14300.1592934943645749No Hit
GAGGTAATGATTAAGAGGGACAGACGGGGGCATTCGTACTGTGCCGTTAGA13710.15272124529638614No Hit
ACACAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTGTGGGTGGT13060.1454806319161782No Hit
TTCACTTTGACATTCAGAGCACTGGGCAGAAATCACATTGCGTCAACACCA13040.1452578438121718No Hit
GGAATCCGACTGTTTAATTAAAACAAAGCTTTGCGATGGCCGTCACCTGGT12210.13601213749590627No Hit
TGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCT12060.13434122671585827No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCTCTCGAGCCTGAATAAT11750.1308880111037591No Hit
AGTAACGGCGAGTGAAGTGGGAAGAGCCCATCGCCGAATCCGCGCGTTCTT10800.12030557616345518No Hit
CTGGAGCGTCGGGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGT10580.1178549070193848No Hit
GACGCTCCAGCGCCATCCATTTTCAGGGCTGATTGATTCGGCAGGTGAGTT10080.11228520441922483No Hit
AACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTG10010.11150544605520243No Hit
TGTGTAACAACTCACCTGCCGAATCAATCAGCCCTGAAAATGGATGGCGCT9670.10771804828709367No Hit
TTGTGAAGAGACATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTC9630.10727247207908087No Hit
CTGGAGCGTCGGGCCTATACCCGGCCGTCGCGGTGGTACAGGCGCCGAGGG9500.10582434940303927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA207.0300174E-445.00317419
GCGGCTC6950.043.37952430
GGCCCGT5900.043.09145728
TAGGCCC17250.042.911292
GTGTCTC13750.042.8709754
CGGCCGC7350.042.85539237
GCCGCGT7250.042.8282239
CTTGGCC13850.042.7238914
CGTGTCT13800.042.7156453
GCGTGTC13850.042.5614432
AGGCCCG17400.042.5413673
CGCACTC1750.042.43156422
GCTAGGC1650.042.2757122
CCCGTTG14000.042.2661336
ACCCGTT14000.042.2661335
GGCTCCG7350.042.2431732
CCAACGC13800.042.2312443
AACCCGT14150.041.9770834
GAGCCGG23450.041.9279212
CGCGGCT7150.041.8514429
Produced by FastQC (version 0.11.2)