FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l06n01 fzgrna-15h-2.3410000000d76d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l06n01 fzgrna-15h-2.3410000000d76d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641300
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCCGCCTGACACGGTCTTCAGATCGAATCGCGCCGCACCCGAAAGCGCG311654.859660065491969No Hit
CGGAATTGTGGCGTACGGAATTCGCTCTGCGCGCGCGTCCTGCTGCCCACG160782.5070949633556836No Hit
TCGCGTCGGGCGCCTTAACCCCGCGTTCGGTTCATCCCGCATCGCCAGTCC133872.087478559176672No Hit
CAACAGGGTCTTCTTTCCCCGCTAATTCTGCCAAGCCCGTTCCCTTGGCTG120931.8857009200062373No Hit
TTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCG115021.7935443630126309No Hit
GCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTCT89311.3926399501013567No Hit
GTGGCCCACTTGGCACTCGCATTCCACGCCCGGCTCCAATCAAGCGAGTCG86621.350693903009512No Hit
GAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCG86551.3496023701855606No Hit
TCGGATTTTCACGGGCCGACGCGGGCTCTCCGGACAGCACAAGAGACGTGC56940.88788398565414No Hit
TGAGCCTGGACCTGTGTCGGCGTCTTCCCGTGGAATGCCGCAGCTAATGCG55960.8726025261188212No Hit
CCCTCGGATTTTCACGGGCCGACGCGGGCTCTCCGGACAGCACAAGAGACG53680.8370497427101201No Hit
TCTTCGAACCTCTGACTTTCGCTCTTGATTAAAGAAAACATTCTTGGCAAA53620.8361141431467332No Hit
ATAGGCCACGCTTTCGCGGTCTGTATTCATACTGAAAATCAAAATCAAGTG53470.833775144238266No Hit
AGCTTTGCGATGGCCGTCACCTGGTGTTGACGCAATGTGATTTCTGCCCAG50350.7851239669421488No Hit
CCCTCAGTAACGGCGAGTGAAGTGGGAAGAGCCCATCGCCGAATCCGCGCG46730.7286761266178077No Hit
TTCCCGTGTTGAGAGCTCTTTCTTGATTCTGTGGGTGGTGGTGCATGGCCG44630.6959301418992672No Hit
GGACCGAAAGGGAATCGGGTTAATATTCCCGAACCTCGACACGGAGATTGG44480.6935911429907999No Hit
GATTCCCGCTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACG39770.6201465772649306No Hit
GTCGCGAGGCGTGTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGG37690.5877124590675191No Hit
CAGTACACGCCTCGCGACGGACCGACCGCCCGCGCCCAAAATCCAACTACG33540.5230001559332607No Hit
CGGGTGAGATTTCCCGTGTTGAGAGCTCTTTCTTGATTCTGTGGGTGGTGG30250.4716981132075472No Hit
TTTAATTAAAACAAAGCTTTGCGATGGCCGTCACCTGGTGTTGACGCAATG30060.4687353812568221No Hit
TTGACAGATTGAGAGCTCTTTCTTGATTCTGTGGGTGGTGGTGCATGGCCG29170.4548573210665835No Hit
CCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTC28860.4500233899890847No Hit
TCAAGTGTCTGCCCTATCAACTTTCGTCGGTACGGTATTCGCCTACCGAGG24610.38375175424918134No Hit
GGCCCTTGGCGGCGGCGGGTGCGCGCTCTCGGCGTCTTCCCGGAGTCGGGG23410.3650397629814439No Hit
CTTTGTGTTTGAGAGCTCTTTCTTGATTCTGTGGGTGGTGGTGCATGGCCG21120.32933104631217835No Hit
TAACTTTGTGTTTGACTCCTCTTCAAAGTGCTTTTCAACTTTCCCTCACGG21100.32901917979104944No Hit
AACTTTGTGTTTGACTCTCTCTTCAAAGTGCTTTTCAACTTTCCCTCACGG18900.29471386246686415No Hit
CGGTTTGCCCGGAGATAGGGACATGTGCCCCGTAAAGCAGCACGTCTCTTG18750.292374863558397No Hit
TCGCGTCGGGCGCCTTAACCCCGCATTCGGTTCATCCCGCATCGCCAGTCC17080.2663340090441291No Hit
GCCCACTTGGCACTCGCATTCCACGCCCGGCTCCAATCAAGCGAGTCGGGC16400.2557305473257446No Hit
ACCGTACCGACGAAAGTTGATAGGGCAGACACTTGAATGATCCGTCGCCGG15500.24169655387494154No Hit
ACAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTGTGGGTGGTGG14040.21893029783252768No Hit
TGGTAATTCTAGAGCTAATACATGCAAACAGCGCCGACTTCGCGGGAGGCG12050.1878995789801965No Hit
ACCGAGGTCCTATTCCATTATTCCATGCACCATTATTCAGGCTCGAGAGCC11720.1827537813815687No Hit
CAACAGGGTCTTCTTTCCCCGCTAGTTCTGCCAAGCCCGTTCCCTTGGCTG11680.18213004833931076No Hit
TAACTTTGTGTTTGACTCTTCTTCAAAGTGCTTTTCAACTTTCCCTCACGG11300.1762045844378606No Hit
GCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGCGGGG10420.1624824575081865No Hit
CAGCCAAGGGAACGGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCTGTTG10350.16139092468423513No Hit
CTTTCCCGCTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACG10190.15889599251520348No Hit
AGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGT9870.15390612817714017No Hit
CGCGACGGCCGGGTATAGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGC9390.1464213316700452No Hit
TTTTCCCGCTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACG9100.14189926711367534No Hit
GACTGAACGCCTCTAAGTCAGAATCCCGCCTGGAAAGCGACGATGCGTTCG8520.1328551380009356No Hit
GGCCCTTGGCGGCGGCGGGTGCGCGCTCTCGGCGTCTTCCCGGAGTCGGGT7990.12459067519101825No Hit
TAACTTTGTGTTTGACCTCTCTTCAAAGTGCTTTTCAACTTTCCCTCACGG7860.12256354280368004No Hit
CGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTCGGGTGCGGCG6450.10057695306408858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAC800.045.0050720
CTACGCC302.1634787E-645.0015624
CTCCTAG207.032453E-444.9980527
CGCGCCG38000.044.4651831
ATCGCGC37800.044.4623629
TCGCGCC38050.044.40674630
CGCCCGC3800.044.4059727
ACACGGT38200.044.3501711
CGAATCG38100.044.34847326
AATCGCG38000.044.34676428
CGCCTGA38300.044.293125
GCGCCGC38150.044.29034432
CGCCGCA37950.044.28661733
CAGATCG33450.044.26507621
CCGCACC37850.044.1658535
CGCTAAT13250.044.1559220
CGCCTTA17850.044.11573811
GAATCGC38350.044.05937227
GCACCCG37850.044.04696737
CACCCGA37450.044.03680838
Produced by FastQC (version 0.11.2)