FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l06n01 fzgrna-15h-1.3410000000d62b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l06n01 fzgrna-15h-1.3410000000d62b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559017
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATCAAGTGAGCTTTTGCCCTTTTGCTCTACGCGAGGTTTCCGTCCTCA171771.1017840087696285No Hit
TAAGAAGCCCGACTCGCTTGATTGGAGCCGGGCGTGGAATGCGAGTGCCAA158491.0166021281358704No Hit
TACCAAGAGTGGCCCACTTGGCACTCGCATTCCACGCCCGGCTCCAATCAA150080.962657879933317No Hit
TAGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTGATTGATTCGGCAG118730.7615696300938348No Hit
AGAATCAAGAAAGAGCTCTCAACACGGGAAATCTCACCCGGCCCGGACACA116780.7490617485248717No Hit
GTGCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC96940.6218020714334738No Hit
CTCTCAACACGGGAAATCTCACCCGGCCCGGACACAGTGAGGATTGACAGA96750.6205833547677799No Hit
AGAATCAAGAAAGAGCTCTCAATCTGTCAATCCTCACTGTGTCCGGGCCGG90800.5824182802368415No Hit
TGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAGGTGAGCTCA75260.4827400855795671No Hit
CGGTCGGCGTTTAACTTCTTAGAGGGACTAGTGGCGTTTAGCCACACGAGA62930.403651788274278No Hit
TCGGGTTAATATTCCCGAACCTCGACACGGAGATTGGTGCTTCGGCGCCTA58660.3762627347873692No Hit
CGGACCTTTCGGCCAAGGAAGAGACACGCTGATCCGGTCAGTGTAGCGCGC49730.31898305149975914No Hit
GGCACGAAGCGGGGTGCGGCGCTGTACCGGACTGGGCGAACGCCTTCGGGT49570.3179567637812801No Hit
TCGATAGGCCACGCTTTCGCGGTCTGTATTCATACTGAAAATCAAAATCAA48690.31231218132964556No Hit
GGGGAGGTCGTCAAATTAACGCTCCCCGATCGCTAGTTGGCATCGTTTATA46680.2994194418662529No Hit
AGCTTTGTTTTAATTAAACAGTCGGATTCCCCCGGTCCGTACCAGTTCTAA42790.2744678217107318No Hit
TAACTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAAC41190.2642049445259417No Hit
GAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTGTCCTAAG40780.2615750822473392No Hit
GCACGTCTCTTGTGCTGTCCGGAGAGCCCGCGTCGGCCCGTGAAAATCCGA37510.24060032700092432No Hit
CCTGTTATTGCTCAATCTCGTGTGGCTAAACGCCACTAGTCCCTCTAAGAA37410.2399588971768749No Hit
CGGTCGGCGTCTAACTTCTTAGAGGGACTAGTGGCGTTTAGCCACACGAGA37040.2375856068278922No Hit
TCACCTGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAA36360.2332238840243564No Hit
AACTTTAAATTGGTAAGAAGCCCGACTCGCTTGATTGGAGCCGGGCGTGGA33440.21449413316211433No Hit
TAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTA32280.20705354720314145No Hit
GCCGCCAAGGGCCTGACACCCGCTAGGGGACGAAGCCTCGATCAGAAGAAC32150.20621968843187727No Hit
CGTGTACTGGTCGACCCGGCCTTACGTCCGGTTCTCCGGCGGTGCTCTTGA31890.20455197088934887No Hit
GTTTCCCGGACCTTTCGGCCAAGGAAGAGACACGCTGATCCGGTCAGTGTA30390.1949305235286081No Hit
GGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTA30240.193968378792534No Hit
GATGGTGGGCGCACGACCGGCCGATCTTCTCCGCGCTGTCGGCGAGGTCGA28650.1837696445901488No Hit
TTATCAGCCAAGCACATGAACCAAATGTCTGAATCTGCGGTTCCTCTCGTA28160.1806266384523068No Hit
GAGCTTGACTCTAGTCCGACTTTGTGAAGAGACATGAGAGGTGTAGGATAA26940.1728011945989043No Hit
TTCCCGGACCTTTCGGCCAAGGAAGAGACACGCTGATCCGGTCAGTGTAGC26490.1699147603906821No Hit
TAGGGGACGAAGCCTCGATCAGAAGAACGTGGGCAGCAGGACGCGCGCGCA25230.16183274460765984No Hit
CTTTAAATTGGTAAGAAGCCCGACTCGCTTGATTGGAGCCGGGCGTGGAAT25170.16144788671323018No Hit
ACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAA24700.1584331665401981No Hit
AACGGGTTTCCCGGACCTTTCGGCCAAGGAAGAGACACGCTGATCCGGTCA23810.15272444110615857No Hit
TAGCCTTAAGCGCGCGCTTTCGGGTGCGGCGCGATTCGATCTGAAGACCGT23390.15003043584515113No Hit
ATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAA22420.1438085665518721No Hit
ACCTGGACCCGAAGGTCGCTCTCCGCTTTACTGAATAAGTAAAAAAACGAT22390.1436161376046573No Hit
GTATTCATACTGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTGCTCTAC21340.13688112445213874No Hit
CGGTGAAATACCACTACTCGCATCGTTTTTTTACTTATTCAGTAAAGCGGA21310.13668869550492393No Hit
GGCGATGCGGGATGAACCGAACGCGGGGTTAAGGCGCCCGACGCGACGCTC20870.13386640427910665No Hit
GTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA20110.1289915376163313No Hit
CGGGTGGAGCAGCCGTCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAA20040.12854253673949675No Hit
GAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTTCGGGGCCGCAC19800.12700310516177823No Hit
CGATGCGGGATGAACCGAACGCGGGGTTAAGGCGCCCGACGCGACGCTCAT18410.11808723060749177No Hit
TTCCACGCCCGGCTCCAATCAAGCGAGTCGGGCTTCTTACCAATTTAAAGT18040.11571394025850905No Hit
CATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTGTGGGCTCTC17640.11314822096231152No Hit
ACCAGACAAATCGCTCCACCAACACGGGAAATCTCACCCGGCCCGGACACA17590.11282750605028681No Hit
CCCGGACCTTTCGGCCAAGGAAGAGACACGCTGATCCGGTCAGTGTAGCGC17590.11282750605028681No Hit
AAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCGCGCTTTC17520.11237850517345224No Hit
AAATTGGTAAGAAGCCCGACTCGCTTGATTGGAGCCGGGCGTGGAATGCGA17400.11160878938459298No Hit
TGCTGTCTATATCAACCAACACCTTTTGTGGGCTCTCATGAGCGTCGCGTC16660.10686220868662755No Hit
TTCGGGTGCGGCGCGATTCGATCTGAAGACCGTGTCAGGCGGGGAGTTTGA16580.10634906482738804No Hit
TTAAATTGGTAAGAAGCCCGACTCGCTTGATTGGAGCCGGGCGTGGAATGC16570.1062849218449831No Hit
TTATTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGC16020.10275705781271148No Hit
CTATTCTCAAACTTTAAATTGGTAAGAAGCCCGACTCGCTTGATTGGAGCC15680.10057619641094358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCAC20450.043.45656211
GGCACTC16700.043.11899620
GTGGCCC20450.043.0164959
GCCCACT20750.042.82827412
AGTGGCC20650.042.5998738
TGGCCCA20900.042.305610
CCACTTG20650.042.27301814
CACTTGG20800.042.1844915
GAGTGGC20900.042.09037
CGCATTC21300.041.9349826
AGAGTGG21050.041.8972476
AAGAGTG21100.041.7979665
GGCCTTA4900.041.78809718
AGACTAG3350.041.6414925
TGGCACT17100.041.58265319
ACTTGGC21100.041.2674816
GCACTCG17500.041.14784221
CAAGAGT21550.041.029564
TACGTCC4400.040.91142723
CTCCGCG4250.040.76310729
Produced by FastQC (version 0.11.2)