FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l06n01 controlgrna-15h-2.3410000000d66e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l06n01 controlgrna-15h-2.3410000000d66e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457444
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGGTTCATCCCGCATCGCCAGTCCTGCTTACCAAGAGTGGCCCACTTGG207381.422902012015556No Hit
CTTGCTAAATAGTTACGCGACCCTCCCGGTCGGCGTTTAACTTCTTAGAGG190301.3057105453108317No Hit
ATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTAAACGC131640.9032250981855906No Hit
CCCGAAGACCTCCCACTTATCCTACACCTCTCATGTCTCTTCACAAAGTCG117530.8064117729394749No Hit
ATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTTCGGGGCCGCACGCG114990.788984002129756No Hit
CTTGCTAAATAGTTACGCGACCCTCCCGGTCGGCGTCTAACTTCTTAGAGG104820.7192043056199758No Hit
CTAGGCTTCGACGCCCACCGCCGCGGCCCTCCTACTCGTCGCGGTCTATCG98110.6731648008431199No Hit
GGCGTTTAACTTCTTAGAGGGACTAGTGGCGTTTAGCCACACGAGATTGAG96650.6631472632910768No Hit
TCCCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAG77150.5293513850274865No Hit
GAACTCTTCCCGGGACCCACGCCAACGTTTCCGAGTTCGCTTGCGTTACCG69400.4761761000765724No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCG63790.43768405509920105No Hit
TTGCTACTACCACCAAGATCTGCACCGACGGCTGCTCCACCCGGGCTCGCG63560.43610594986839973No Hit
CCAGTTCTAAGTCAGCTGTTCGACGCCGGCCGAAGCGGGCCGCGAAGCCCG55490.3807350402485447No Hit
GGCGTCTAACTTCTTAGAGGGACTAGTGGCGTTTAGCCACACGAGATTGAG55170.37853941557960374No Hit
CCCACGCCAACGTTTCCGAGTTCGCTTGCGTTACCGCACTAGGCGCCGAAG54670.3751087520343835No Hit
CGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG54120.3713350221346412No Hit
TTACACCATCTCCCTCGGATTTTCACGGGCCGACGCGGGCTCTCCGGACAG53590.36769851877670773No Hit
CGGTCTGTATTCATACTGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTG47840.3282458880066747No Hit
GGTCGTCAAATTAACGCTCCCCGATCGCTAGTTGGCATCGTTTATAGTCAG44530.3055348953373166No Hit
ACCTGGCGGATCGCACGGTCTAGCACCGGCGACGGATCATTCAAGTGTCTG31520.21626902989068533No Hit
GTCTGATAAAAGCACGCCTCCCGCGAAGTCGGCGCTGTTTGCATGTATTAG25760.17674778584974793No Hit
TTGGGGGCGAAACGCCCTCAACCTATTCTCAAACTTTAAATTGGTAAGAAG24310.16679886156860915No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAGGGGCTGGGTCGGTCGGGCCGTGG22440.15396817990948536No Hit
AGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAAGAGTAACGCAGGTG22420.15383095336767655No Hit
CTCACTGAGCTCACCTTAGGACACCTGCGTTACTCTTTGACAGATGTACCG21950.1506061296351695No Hit
CGCTCCGAAAACCAACAAAATAGAACCGAGGTCCTATTCCATTATTCCATG21780.14943970402979465No Hit
ACATGAGAGGTGTAGGATAAGTGGGAGGTCTTCGGGCCGTCGGTGAAATAC19560.13420755788901667No Hit
GCCCGACTCGCTTGATTGGAGCCGGGCGTGGAATGCGAGTGCCAAGTGGGC19290.13235499957459773No Hit
AAACGCCACTAGTCCCTCTAAGAAGTTAAACGCCGACCGGGAGGGTCGCGT19030.1305710545310832No Hit
CATCGTTTTTTTACTTATTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGG16560.11362357661769508No Hit
TTCAGTAAAGCGGAGAGCGACCTTCGGGTCCAGGTTCTAGCCTTAAGCGCG16420.11266299082503342No Hit
GGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCG16320.11197685811598936No Hit
TTTGGGCTGCATTCCCAAACAACCCGACTCCGGGAAGACGCCGAGAGCGCG16210.1112221121360409No Hit
GCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC15700.10772283531991624No Hit
CATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTAAACGCCACTAGTCC15480.10621334336001931No Hit
GCTCTACCGACGACCCTTCGGGCCGACTAACTATGCCTCTCGAGGCCGAGG14820.10168486748032858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCAC31150.043.26638841
GCCCACT31150.043.198642
CCTACTC10450.043.05917731
TGGCCCA31150.043.04821840
GCTTACC31700.042.9386328
CTAGGCT10650.042.9108161
CGCCAGT31350.042.85123418
CCGCATC31750.042.80014812
GTGGCCC31500.042.7841839
GAGTGGC31400.042.7756637
CCGGGCT10050.042.7611341
CCTCCTA10600.042.662128
TCGCCAG31550.042.65091717
GCCAGTC26100.042.59345219
AGTGGCC31600.042.57758738
CAGTCCT25800.042.56537621
ATCGCCA31550.042.5082716
CGGTTCA31450.042.4930423
CTCCTAC10600.042.44985229
ATCCCGC31700.042.441829
Produced by FastQC (version 0.11.2)