FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l06n01 controlgrna-15h-1.3410000000d589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l06n01 controlgrna-15h-1.3410000000d589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868616
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTTCAGATCGAATCGCGCCGCACCCGAAAGCGCGCGCTTAAGGCTAGA283910.9897107176422358No Hit
ACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCA245510.8558482557442335No Hit
TTGATTGGAGCCGGGCGTGGAATGCGAGTGCCAAGTGGGCCACTCTTGGTA170180.5932477543177616No Hit
GCTACACTGACCGGATCAGCGTGTCTCTTCCTTGGCCGAAAGGTCCGGGAA114970.40078560532326396No Hit
AGTACGAATGCCCCCGTCTGTCCCTCTTAATCATTACCTCGCGCTCCGAAA94390.3290436921498033No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA86430.30129511931886316No Hit
ATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGA76020.2650058425387016No Hit
ATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAACA75600.2635417218616922No Hit
GACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAA58850.2051511948619125No Hit
GCCCGACGCGACGCTCATGAGAGCCCACAAAAGGTGTTGGTTGATATAGAC56630.19741227128343425No Hit
TCACTGAGCTCACCTTAGGACACCTGCGTTACTCTTTGACAGATGTACCGC56120.1956344104613514No Hit
TGAGAATAGGTTGAGGGCGTTTCGCCCCCAAGACCTCTAATCATTCGCTTT55810.19455374996165398No Hit
CGCCCAGTCCGGTACAGCGCCGCACCCCGCTTCGTGCCACGGCCCGACCGA53680.18712856652824916No Hit
GTCGTCAAATTAACGCTCCCCGATCGCTAGTTGGCATCGTTTATAGTCAGA53460.1863616461736252No Hit
CCCTCTAAGAAGTTAAACGCCGACCGGGAGGGTCGCGTAACTATTTAGCAA50780.17701916185366046No Hit
ATGATCCGTCGCCGGTGCTAGACCGTGCGATCCGCCAGGTTATCCAGAGTC50200.1749972809187427No Hit
GGCCATCGCAAAGCTTTGTTTTAATTAAACAGTCGGATTCCCCCGGTCCGT44360.15463903150508818No Hit
GACCAAAAACCGACCGCGGCTCCGGCCGCGTTCGACTGGTGACTCTGGATA43460.15150163005435374No Hit
GACACTTGAATGATCCGTCGCCGGTGCTAGACCGTGCGATCCGCCAGGTTA43250.15076956971584904No Hit
TTCGCGGCCCGCTTCGGCCGGCGTCGAACAGCTGACTTAGAACTGGTACGG35200.12270725673983551No Hit
AAGTAAACTTTCCGGCCGCCGCCGACACTCAGTCAAGAGCACCGCCGGAGA34730.12106883598222976No Hit
TAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGA34420.11998817548253234No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTAAACGCC32770.11423627282285255No Hit
AAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTA32610.11367851256494423No Hit
GCGATTCGATCTGAAGACCGTGTCAGGCGGGGAGTTTGACTGGGGCGGTAC30440.1061138890670623No Hit
GCATGAATGGATTAACGAGATTCCCGCTGTCCCTATCTACTATCTAGCGAA29020.10116376677812575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCT13250.042.10968413
CCCCGTC13500.041.82983412
CCCCCGT13400.041.80620611
CCGTCTG13600.040.86055414
CGTCCTG30100.040.73650433
AGGCTAG39000.040.50226244
TGCCCCC13950.040.3192069
CCACCGT30550.040.28304729
GCCCCCG14050.040.03223810
GGCTAGA39400.040.0311745
ATGCCCC14100.039.890288
GCTTAAG39500.039.6450239
CGCGCCG39500.039.5942717
CACCGTC31150.039.50712630
AAAGCGC39300.039.44326430
CCCGACC8500.039.44243
TCGCGCC39700.039.3941116
CGCTTAA39850.039.183938
CACGGCC8500.039.17592238
GCACCCG37750.039.10015523
Produced by FastQC (version 0.11.2)