FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8441b-062415.3410000000d893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8441b-062415.3410000000d893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249329
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC44331.777972077054815No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA15040.6032190399030999No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC13590.5450629489549952TruSeq Adapter, Index 5 (100% over 51bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC12180.4885111639640796No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG11740.47086379843499954TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG4960.19893393869144785TruSeq Adapter, Index 19 (97% over 40bp)
CCGCACCTTTTCCTCTGTCCACTTTCAACCGTCCCTCCAAATGTAAAATGG3230.12954770604301946No Hit
CAAGCACGCCCGCTGCGTCTGACCAAGGCCCTCACTACCCGACCCTTAGAG2910.1167132583855067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTC253.882706E-544.99859628
CTCGGGG4650.044.5147416
TCGGGGA5600.044.19504517
CCTCGGG4700.044.0411815
GGCGGTC5650.043.8127338
CAGGCGG5750.043.4334336
ACGTCTG4750.043.1039215
CCCAGGC5700.043.02497534
AGGCGGT5800.042.6796437
TCCTCGG4850.042.6790814
AGCACAC4650.042.57931510
CGTCTGA5200.042.40252316
GCGGTCT5850.042.31485739
GATCGGA4800.042.1861881
CACGTCT4750.042.15657814
CACACGT4700.042.12634712
CCAGGCG5950.041.9734835
GTCTCCC5900.041.9562642
ACACGTC4800.041.71745313
GCACACG4750.041.6829111
Produced by FastQC (version 0.11.2)