##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C6001ACXX l05n01 ah8441a-062415.3410000000d850.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 148521 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49721588192916 33.0 33.0 33.0 33.0 33.0 2 32.49567401242922 33.0 33.0 33.0 33.0 33.0 3 32.523185273463014 33.0 33.0 33.0 33.0 33.0 4 36.33025632738805 37.0 37.0 37.0 37.0 37.0 5 36.37177907501296 37.0 37.0 37.0 37.0 37.0 6 36.26207741666162 37.0 37.0 37.0 33.0 37.0 7 36.21052241770524 37.0 37.0 37.0 33.0 37.0 8 36.20634119080803 37.0 37.0 37.0 33.0 37.0 9 36.222163869082486 37.0 37.0 37.0 33.0 37.0 10 36.17670228452542 37.0 37.0 37.0 33.0 37.0 11 36.18237824954047 37.0 37.0 37.0 33.0 37.0 12 36.16331023895611 37.0 37.0 37.0 33.0 37.0 13 36.170810861763655 37.0 37.0 37.0 33.0 37.0 14 38.084802822496485 40.0 37.0 40.0 33.0 40.0 15 38.03335555241346 40.0 37.0 40.0 33.0 40.0 16 38.031288504655905 40.0 37.0 40.0 33.0 40.0 17 38.0162468607133 40.0 37.0 40.0 33.0 40.0 18 37.943233616794934 40.0 37.0 40.0 33.0 40.0 19 37.97114886110381 40.0 37.0 40.0 33.0 40.0 20 37.95283495263296 40.0 37.0 40.0 33.0 40.0 21 37.913069532254696 40.0 37.0 40.0 33.0 40.0 22 37.83611745140418 40.0 37.0 40.0 33.0 40.0 23 37.78004457282135 40.0 37.0 40.0 33.0 40.0 24 37.7925747874038 40.0 37.0 40.0 33.0 40.0 25 37.73136458817272 40.0 37.0 40.0 33.0 40.0 26 37.62706957265303 40.0 37.0 40.0 33.0 40.0 27 37.56853912914672 40.0 37.0 40.0 33.0 40.0 28 37.44314945361262 40.0 37.0 40.0 33.0 40.0 29 37.36353108314649 40.0 37.0 40.0 33.0 40.0 30 37.309020273227354 40.0 37.0 40.0 33.0 40.0 31 37.1962954733674 40.0 37.0 40.0 33.0 40.0 32 37.14984412978636 40.0 37.0 40.0 33.0 40.0 33 37.10244342550885 40.0 37.0 40.0 33.0 40.0 34 37.031396233529264 40.0 37.0 40.0 33.0 40.0 35 36.93427192114247 40.0 37.0 40.0 33.0 40.0 36 36.835046895725185 40.0 37.0 40.0 33.0 40.0 37 36.80546185387925 40.0 37.0 40.0 33.0 40.0 38 36.73421267026212 40.0 37.0 40.0 33.0 40.0 39 36.57354178870328 40.0 37.0 40.0 33.0 40.0 40 36.563334477952615 40.0 37.0 40.0 33.0 40.0 41 36.42923222978569 40.0 37.0 40.0 33.0 40.0 42 36.391439594400794 40.0 37.0 40.0 33.0 40.0 43 36.33028999266097 40.0 37.0 40.0 33.0 40.0 44 36.28577103574579 40.0 37.0 40.0 33.0 40.0 45 36.23180560324803 40.0 37.0 40.0 33.0 40.0 46 36.10495485486901 40.0 37.0 40.0 27.0 40.0 47 35.79650689128137 37.0 37.0 40.0 27.0 40.0 48 35.85001447606736 37.0 37.0 40.0 27.0 40.0 49 35.786198584711926 37.0 37.0 40.0 27.0 40.0 50 35.71555537600743 37.0 37.0 40.0 27.0 40.0 51 34.73275833047178 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1306 1 0.3011964698426439 1306 2 0.2947018927911884 1306 3 0.17288071180127673 1306 4 0.3064244676432537 1306 5 0.17796238054814495 1306 6 0.34223394091436177 1306 7 0.46985692385206335 1306 8 0.5770167024044355 1306 9 0.4586090514898373 1306 10 0.419396385442262 1306 11 0.4596288540950937 1306 12 0.33604111449791674 1306 13 0.3385180835289603 1306 14 0.5337776808379857 1306 15 0.4438982247997387 1306 16 0.6428843625840059 1306 17 0.568945872023491 1306 18 0.6149073187951473 1306 19 0.5283707145225662 1306 20 0.7238120050020882 1306 21 0.39963910889494514 1306 22 0.669485427376479 1306 23 0.5555871957886254 1306 24 1.05190996791805 1306 25 0.992021848015689 1306 26 1.101205202157324 1306 27 0.9876959334205395 1306 28 1.0989023889365654 1306 29 1.1587900566635767 1306 30 1.0453437731003845 1306 31 0.8398781690451003 1306 32 0.9007007072690527 1306 33 1.0579167334854631 1306 34 1.158625693290361 1306 35 1.174998560221617 1306 36 1.4510919744049673 1306 37 1.673586964298309 1306 38 1.8876749203983323 1306 39 1.8296162926646389 1306 40 1.4106716803489974 1306 41 1.3767072890802368 1306 42 1.3666143028626152 1306 43 1.416105477889566 1306 44 1.5876167041304186 1306 45 1.1793060008649903 1306 46 1.3840182202743208 1306 47 1.7721671122088196 1306 48 1.6794018600155525 1306 49 1.6168475599213323 1306 50 1.6817054568412644 1306 51 1.2109052133959537 1101 1 0.1672251779766114 1101 2 0.33173973533142487 1101 3 0.3226632259378519 1101 4 0.3814933381666705 1101 5 0.3696399215079822 1101 6 0.5251540511071937 1101 7 0.4436933744827698 1101 8 0.3670558498598524 1101 9 0.3999603636545501 1101 10 0.5502213818175008 1101 11 0.4706336387840935 1101 12 0.5083914117285957 1101 13 0.38471933044791484 1101 14 0.9248027642075698 1101 15 0.8953191324407399 1101 16 0.8298787079515577 1101 17 0.929537433571987 1101 18 0.8250494095592487 1101 19 1.0586157485953507 1101 20 0.9539410466143821 1101 21 0.9164725147235657 1101 22 1.1244803527063354 1101 23 1.0053552108676485 1101 24 1.216372777835403 1101 25 1.2398324903239342 1101 26 1.1571933129301257 1101 27 1.110002733478531 1101 28 1.2683746232413355 1101 29 1.569214879050115 1101 30 1.4500849649229153 1101 31 1.4866738773240584 1101 32 1.6423130024698622 1101 33 1.8692694955559332 1101 34 1.8633596355841107 1101 35 1.7629933405608966 1101 36 1.7323519425070444 1101 37 1.9733549793773264 1101 38 2.06166361113344 1101 39 2.3893669088745852 1101 40 2.1987389559760757 1101 41 2.4016456680855995 1101 42 2.507218913562916 1101 43 2.3306045354508242 1101 44 2.4916764402475664 1101 45 2.4609791921075583 1101 46 2.4593988205353057 1101 47 2.5286771890538233 1101 48 2.390682126798211 1101 49 2.544569757978458 1101 50 2.906709081605655 1101 51 2.265291178308239 1305 1 -0.49066955886549124 1305 2 -0.5708918436159429 1305 3 -0.3273367740621467 1305 4 0.15780912764035548 1305 5 0.15911360571850963 1305 6 0.514627735317724 1305 7 0.2936933744827712 1305 8 -0.8592599396138283 1305 9 -0.36319753108229236 1305 10 0.07127401339644734 1305 11 -0.12410320332116953 1305 12 0.1452335169917589 1305 13 0.2505088041321244 1305 14 0.198486974733882 1305 15 -0.05994402545399424 1305 16 -1.138542344680026 1305 17 -0.2283573032701156 1305 18 -0.9091611167565432 1305 19 0.31124732754272344 1305 20 0.11709894135122312 1305 21 0.14278830419724642 1305 22 -1.5176249104515591 1305 23 -1.6446447891323572 1305 24 -0.7625745905856505 1305 25 -0.3154306675708014 1305 26 0.601930155035383 1305 27 0.5389501018995873 1305 28 0.37626936008344103 1305 29 -0.29394301568672176 1305 30 0.8711375965018604 1305 31 -0.10016822793910052 1305 32 0.4423130024698665 1305 33 -0.014941030759857199 1305 34 1.1870438461104271 1305 35 0.5893091300345787 1305 36 0.5507729951386224 1305 37 0.6312497162194362 1305 38 -1.2804416520244573 1305 39 0.35252480361142347 1305 40 0.5882126401866046 1305 41 -1.1509859108617704 1305 42 -1.4533074022265566 1305 43 -1.9378165171807566 1305 44 -0.6451656650155897 1305 45 -0.7705997552608608 1305 46 -0.10902223209627238 1305 47 1.2102561364222453 1305 48 0.10647160048242199 1305 49 -1.0606933999162749 1305 50 0.06723539739513029 1305 51 -0.08734040063912829 1102 1 -0.062439893793722945 1102 2 -0.37471959481211314 1102 3 -0.8105903625788926 1102 4 -0.5806278739545405 1102 5 -0.08650042936920954 1102 6 -0.0021186761655300757 1102 7 0.524953342584844 1102 8 -0.1750494132980407 1102 9 0.5522729633355681 1102 10 0.5130602972879927 1102 11 -0.4494620549958128 1102 12 0.17075185829956752 1102 13 -0.36671607625065406 1102 14 0.4704167992952861 1102 15 0.1601516683259092 1102 16 0.4252535085895133 1102 17 -0.9875279571776119 1102 18 0.26229023890534364 1102 19 -0.7581306628879005 1102 20 -1.4690254605626478 1102 21 -0.534245188625718 1102 22 -1.4131592007226956 1102 23 -1.7116304350653664 1102 24 -0.4108999494373222 1102 25 0.20689788107353735 1102 26 -0.40843667112091 1102 27 -0.6045905679568762 1102 28 -2.3143207515593076 1102 29 -0.0698600810774721 1102 30 -0.7755928660180729 1102 31 -0.17114111470145588 1102 32 0.04119657503764529 1102 33 0.4132886343119111 1102 34 0.30187638199559785 1102 35 0.05103161807285517 1102 36 0.4207889441019361 1102 37 0.8003087273837082 1102 38 0.30089806089420534 1102 39 -0.6387032665640149 1102 40 -0.5975927824609215 1102 41 -0.7307307274486945 1102 42 -0.4405482315726488 1102 43 0.31417710323391645 1102 44 -0.2966808165307384 1102 45 0.523658617944875 1102 46 -0.4176346722876616 1102 47 0.17437096895812942 1102 48 -0.08092871849684258 1102 49 -0.9203425227232955 1102 50 -0.1943276010099737 1102 51 -0.3042463017555619 1304 1 0.045966894786729995 1304 2 0.12047840545100996 1304 3 0.15533517353478032 1304 4 0.1032733671102335 1304 5 0.011425282159976291 1304 6 0.0305086097179057 1304 7 0.02352809152138491 1304 8 0.17661484140528927 1304 9 0.06821384830684707 1304 10 0.04057860628242338 1304 11 0.22123916852741132 1304 12 0.12124082904903588 1304 13 0.06717420134440033 1304 14 0.08884419919881026 1304 15 0.09581802801542239 1304 16 0.28338621042698975 1304 17 0.239766697034554 1304 18 0.18936123833524476 1304 19 0.15582802675820062 1304 20 0.3632334527382355 1304 21 0.10238157101117196 1304 22 0.4968087859457313 1304 23 0.420325886471268 1304 24 0.413333704029327 1304 25 0.40217920370652394 1304 26 0.30392573733411155 1304 27 0.06374254610706487 1304 28 0.24183414034088457 1304 29 0.2493961571367933 1304 30 0.3097467822738196 1304 31 0.2791066597141878 1304 32 0.30936304436185935 1304 33 0.3871078686231897 1304 34 0.3952851440005958 1304 35 0.4921577856823802 1304 36 0.33601939623542876 1304 37 0.5701635710446311 1304 38 0.6780852731031217 1304 39 0.8292176210384952 1304 40 0.6318945656950206 1304 41 0.8970375486553266 1304 42 0.9391825056110576 1304 43 1.0001833304847167 1304 44 0.9286404131320225 1304 45 0.8409852854886211 1304 46 1.054013795019273 1304 47 1.285014305361237 1304 48 0.7282630545154802 1304 49 0.987520477759098 1304 50 1.08993324186158 1304 51 0.7005337710418829 1103 1 -0.05612862751199543 1103 2 -0.05794774037408956 1103 3 0.029345181935674702 1103 4 0.18393056120950746 1103 5 0.16362266270365922 1103 6 -0.08577652496370547 1103 7 0.09824907688501128 1103 8 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0.82086092287215 1103 45 0.4781369313596784 1103 46 0.8383073177754525 1103 47 0.7513818458212995 1103 48 0.6077154782714302 1103 49 0.6138770735347094 1103 50 0.9111287348747226 1103 51 0.6727466708060987 1311 1 0.008953828762791716 1311 2 0.010221045942479634 1311 3 0.031009897626020688 1311 4 0.06977598575164023 1311 5 -0.07994109722848464 1311 6 0.11155834448823754 1311 7 0.09474788912357468 1311 8 0.17199888723862955 1311 9 0.11312296604603489 1311 10 -0.06920240125377575 1311 11 -0.015732993359776515 1311 12 -0.02282975388668973 1311 13 -0.0629268422288618 1311 14 0.02736372833523859 1311 15 -0.17876522873052636 1311 16 0.17475207204156362 1311 17 0.1646377066159559 1311 18 0.05586100922030113 1311 19 -0.09525865117868193 1311 20 -0.20231542641075606 1311 21 -0.10506971293861511 1311 22 -0.25611469484097427 1311 23 0.03824759388242427 1311 24 -0.016364252576046567 1311 25 -0.4533178721654707 1311 26 -0.45988135047257117 1311 27 -0.5315705647043103 1311 28 -0.46404699430880214 1311 29 -0.6823716005657374 1311 30 -0.6969350896858799 1311 31 -0.884698874770848 1311 32 -0.7149697392681986 1311 33 -0.7246199687129717 1311 34 -0.7311371161341782 1311 35 -0.9170932802845968 1311 36 -0.7555540927062125 1311 37 -0.908153353171393 1311 38 -0.9376773172160568 1311 39 -0.7955309350195847 1311 40 -0.5894071696241454 1311 41 -0.8473892627118786 1311 42 -0.9890385009931464 1311 43 -0.963049546085756 1311 44 -0.9734304235129088 1311 45 -0.842579794805161 1311 46 -0.6539332574234535 1311 47 -0.7174813647546685 1311 48 -0.7461317325370658 1311 49 -0.8803902269570472 1311 50 -0.8017976503404896 1311 51 -0.7491424908377979 1310 1 0.06400056715378355 1310 2 0.15937131427879336 1310 3 -0.046665906753027286 1310 4 -0.1132249716924818 1310 5 0.046715533072102744 1310 6 0.008604755332548564 1310 7 -0.10938297837119393 1310 8 -0.1926550471008568 1310 9 0.10983805083023412 1310 10 -0.1265577138088929 1310 11 -0.16088971184600354 1310 12 -0.15804669056939957 1310 13 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-0.10950957510577553 1209 25 -0.23103438274107901 1209 26 -0.05435467468597466 1209 27 -0.13485794763602144 1209 28 -0.00936531483916525 1209 29 -0.1823021488136618 1209 30 -0.10750017439596604 1209 31 -0.08586482236633941 1209 32 -0.19737740000691417 1209 33 -0.0859627025864853 1209 34 -0.1684979495552028 1209 35 -0.23452987925334412 1209 36 -0.2289793268428042 1209 37 -0.19512799275889137 1209 38 -0.140813169052322 1209 39 0.22874771382814174 1209 40 -0.05841274681029773 1209 41 0.3341534080236812 1209 42 0.26248207145765434 1209 43 0.6319667645529918 1209 44 -0.0745774297214723 1209 45 -0.07505795959522743 1209 46 -0.14041542095075954 1209 47 -0.4446664641969491 1209 48 -0.5037451177838292 1209 49 -0.4293621305664317 1209 50 0.09708059863351792 1209 51 0.2712973702587007 1214 1 -0.05443028225987945 1214 2 -0.13302956677847533 1214 3 -0.26914062289126406 1214 4 -0.13062119927826643 1214 5 -0.3052963177686223 1214 6 -0.2770485920646095 1214 7 -0.30751228283232024 1214 8 -0.18606262915707106 1214 9 -0.43437768410802846 1214 10 -0.34583704619085154 1214 11 -0.3309198042949717 1214 12 -0.3751699144944496 1214 13 -0.3242651351212942 1214 14 -0.5574833475475884 1214 15 -0.5949544590269156 1214 16 -0.7336037868316581 1214 17 -0.577302353119407 1214 18 -0.47686341214258476 1214 19 -0.5881266580821318 1214 20 -0.7501628254004515 1214 21 -0.5438567224663231 1214 22 -0.5802263479660539 1214 23 -0.4985747682652075 1214 24 -0.373054534939925 1214 25 -0.4346052560243123 1214 26 -0.4481509949762099 1214 27 -0.8022277325543925 1214 28 -0.4283909691537531 1214 29 -0.5160621902751785 1214 30 -0.841939148223382 1214 31 -0.9123639153956233 1214 32 -0.7578956689885175 1214 33 -0.7813449259604113 1214 34 -0.6823046352250657 1214 35 -0.8734777932206939 1214 36 -0.9917727966536347 1214 37 -0.9576675107687436 1214 38 -0.609794770503477 1214 39 -0.7238721358738545 1214 40 -1.1453834646128414 1214 41 -1.0475776103748728 1214 42 -0.747650087132655 1214 43 -1.0886396101554823 1214 44 -0.8125665460728584 1214 45 -0.8946433444099497 1214 46 -1.169536955026949 1214 47 -1.0110515380502818 1214 48 -1.0355409198050793 1214 49 -1.062780114500093 1214 50 -1.0496206192985866 1214 51 -0.64518876604604 1215 1 -0.13189218063731545 1215 2 -0.1302249590131339 1215 3 -0.17347385589717135 1215 4 -0.308465253965835 1215 5 -0.12583790348929824 1215 6 -0.16925588678100212 1215 7 -0.44173214583977227 1215 8 -0.3489624567762988 1215 9 -0.29432970935405933 1215 10 -0.6378902014885952 1215 11 -0.5121385089766122 1215 12 -0.8360239745632185 1215 13 -0.6319146457970461 1215 14 -0.726270571305129 1215 15 -0.5701325402191699 1215 16 -0.3266757216005871 1215 17 -0.6907088342715326 1215 18 -0.8544046603982522 1215 19 -0.8258889915157965 1215 20 -0.7618920142987662 1215 21 -0.6927243905831801 1215 22 -0.6808045988028724 1215 23 -0.63483766262042 1215 24 -0.4289688370714302 1215 25 -0.41083221491742705 1215 26 -0.38058046935167056 1215 27 -0.3356276468200363 1215 28 -1.0621091949999197 1215 29 -0.5550850010850041 1215 30 -0.5068181624607604 1215 31 -0.6064883761356725 1215 32 -0.19934549159137305 1215 33 -0.35181582644472087 1215 34 -0.3518032716840551 1215 35 -0.16861829722923005 1215 36 -0.2799451044582 1215 37 -0.40089586512522857 1215 38 -0.25434379869547996 1215 39 -0.09004902225759537 1215 40 -0.5366102866958187 1215 41 -0.4228067673508207 1215 42 -0.21619287678250743 1215 43 -0.0643284491846785 1215 44 0.3165320575516972 1215 45 -0.019537316555407358 1215 46 0.17750931416067317 1215 47 -0.06428892187567214 1215 48 0.17354144901680257 1215 49 -0.18949366143964852 1215 50 -0.19733908228232622 1215 51 -0.027190024699280002 1212 1 -0.04667284833917762 1212 2 -0.11562868572120522 1212 3 -0.28005058985161924 1212 4 -0.5903816618333266 1212 5 -0.43389626270254666 1212 6 -0.6998459488928006 1212 7 -0.4466849149909109 1212 8 -0.2672369132980421 1212 9 -0.25686529424019255 1212 10 -0.16482796028776647 1212 11 -0.08298478226853945 1212 12 -0.28100003563982057 1212 13 -0.1164319853415634 1212 14 -0.5618748673713796 1212 15 -0.6078881044013613 1212 16 -0.5189699762589726 1212 17 -0.7147059874806416 1212 18 -0.6626150641249637 1212 19 -0.48867043561517676 1212 20 -0.6597925060171974 1212 21 -0.6637906431711684 1212 22 -0.5195985946620851 1212 23 -0.5369145259744599 1212 24 -0.46956472216459844 1212 25 -0.4581115886234315 1212 26 -0.5390237923330332 1212 27 -0.4558689770477855 1212 28 -0.42364840307445917 1212 29 -1.1588278841077795 1212 30 -0.5666913508665559 1212 31 -0.6833096753075196 1212 32 -0.24156857647750485 1212 33 -0.18969432023354216 1212 34 -0.5411633907316755 1212 35 -0.28774679101805134 1212 36 -0.6085197680192778 1212 37 -0.5142272574647748 1212 38 -0.18915875728761478 1212 39 -0.5225574332306806 1212 40 -0.7432840703397119 1212 41 -0.8074826213880826 1212 42 -0.8406429285423442 1212 43 -1.0228494119175977 1212 44 -1.0357906650155897 1212 45 -0.7611425184187581 1212 46 -0.6690551268331149 1212 47 -0.6075892583145972 1212 48 -0.5426237942544176 1212 49 -0.7995091893899584 1212 50 -0.5095738131311833 1212 51 -0.4948239532707106 1213 1 0.06565927975999841 1213 2 -0.027612156795584042 1213 3 0.02412038122275817 1213 4 0.13562556957162997 1213 5 -0.138842027165353 1213 6 0.1796995056526498 1213 7 0.1806007255049522 1213 8 0.1513968676936983 1213 9 -0.2135672570501086 1213 10 0.20176553144776932 1213 11 9.511681446809916E-4 1213 12 0.2561513073353865 1213 13 0.21179632044356111 1213 14 -0.15492755332179087 1213 15 -0.8357161002691313 1213 16 -0.5058759679945055 1213 17 -0.7368392519434508 1213 18 -0.9043764277613278 1213 19 -0.7085438860283944 1213 20 -0.8657196754386831 1213 21 -1.3689559324273688 1213 22 -1.4062721483810918 1213 23 -1.294275063164541 1213 24 -2.014205734561287 1213 25 -2.9942040473011176 1213 26 -3.554442180762784 1213 27 -4.831863295229603 1213 28 -4.114596233652968 1213 29 -5.062973028735875 1213 30 -5.900937218635153 1213 31 -7.500342216629832 1213 32 -8.056599568213041 1213 33 -9.053653514448417 1213 34 -9.351016179987873 1213 35 -10.54814193564615 1213 36 -9.853315739093656 1213 37 -10.246523228539157 1213 38 -11.062738302742162 1213 39 -11.354956710090182 1213 40 -11.761504628190949 1213 41 -11.64119904700792 1213 42 -12.36479065581507 1213 43 -12.432379370595285 1213 44 -13.426157400552778 1213 45 -13.302787663046857 1213 46 -14.103585085510801 1213 47 -14.953728204595588 1213 48 -14.702140839708967 1213 49 -15.115934809224669 1213 50 -15.493225672635315 1213 51 -14.538406081369885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 5.0 11 3.0 12 5.0 13 3.0 14 2.0 15 1.0 16 2.0 17 6.0 18 23.0 19 39.0 20 91.0 21 186.0 22 295.0 23 466.0 24 679.0 25 820.0 26 1043.0 27 1267.0 28 1677.0 29 2034.0 30 2554.0 31 3307.0 32 4029.0 33 5110.0 34 6714.0 35 8880.0 36 12539.0 37 21505.0 38 70101.0 39 5130.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.320720392941606 24.92942278279735 27.854924234767786 25.89493258949326 2 17.721399667387104 34.94926643370298 26.394247278162688 20.935086620747235 3 17.09657220191084 26.51274903885646 36.40697275132809 19.983706007904605 4 16.974030608466144 25.25905427515301 28.617501902087923 29.14941321429293 5 26.545741006322338 25.988917392153297 28.787174877626732 18.67816672389763 6 25.850889773163395 27.149696002585493 26.511402427939483 20.488011796311632 7 17.698507281798534 34.469199641801495 27.87282606500091 19.959467011399063 8 18.345553827404878 34.850963836763825 27.316675756290355 19.486806579540943 9 25.908794042593303 26.12627170568472 28.17312029948627 19.79181395223571 10 18.63520064637759 33.42176137893886 26.354026393751685 21.58901158093186 11 25.283966577117038 27.144309558917595 26.388860834494785 21.18286302947058 12 18.56572471233024 26.787457665919295 27.17393499909104 27.472882622659423 13 17.495169033335355 32.75631055540968 27.062839598440625 22.68568081281435 14 17.883666282882555 27.47086270628396 26.71070084365174 27.934770167181743 15 17.763817911271808 33.282162118488294 27.324082116333713 21.62993785390618 16 18.384605543997147 26.49726301331125 26.8830670410245 28.235064401667103 17 24.190518512533583 25.803758391069277 27.622356434443613 22.383366661953527 18 19.467954026703293 25.983530948485402 33.962873937019005 20.585641087792297 19 17.869526868254322 25.985550864860862 28.82218676146808 27.32273550541674 20 19.439675197446824 26.149837396731773 32.552299001488 21.858188404333394 21 24.314406716895252 25.98083772665145 28.688872280687576 21.015883275765717 22 20.184351034533837 31.78136425151999 26.52554184256772 21.50874287137846 23 17.843315490017844 33.94269939063394 27.386459280207387 20.82752583914083 24 18.152988466277495 27.848587068495362 28.618175207546408 25.38024925768073 25 18.685107127947536 25.249471430302865 31.526994088099435 24.53842735365016 26 18.204895128438206 26.362993637006365 29.226677439989228 26.205433794566208 27 18.254657590509087 27.13824980979121 26.852094989934084 27.754997609765624 28 19.672638886083448 29.45644050336316 26.89316662290181 23.977753987651578 29 22.581991772207296 29.3958430120993 27.797415853650325 20.224749362043077 30 20.929449629001198 25.981362528447733 31.145046391683163 21.944141450867907 31 17.63533530837598 27.557904659305144 29.213573929437114 25.593186102881766 32 18.163761353613296 29.341978575420313 27.639189070905797 24.855071000060597 33 18.010921014536667 30.345877014024953 26.96184377966752 24.68135819177086 34 17.710746027471046 27.695259897656882 26.688661459736064 27.905332615136007 35 20.24966166400711 27.859359955831163 27.809535351903097 24.081443028258633 36 19.18449242868012 26.60162535937679 30.492657603975193 23.721224607967898 37 19.123350390519793 28.52679773767843 29.18394828979262 23.165903582009157 38 21.933598615683977 27.206926966556917 28.562964159950443 22.29651025780866 39 19.930514876684104 27.294456676160273 30.997636697840708 21.777391749314912 40 22.15863183409642 27.95448424454619 27.219229733369243 22.667654187988152 41 18.624929300546743 34.06905653262948 27.782865145842873 19.523149020980902 42 18.231032346683186 26.18438417409572 34.361954267553664 21.222629211667428 43 17.88248919293804 25.90126183390118 27.823639522199926 28.392609450960855 44 18.60021951531557 26.40513369560094 33.51042697748958 21.48421981159391 45 18.192835981685967 26.059116617290602 27.860894155669268 27.887153245354163 46 24.90489560398866 26.417139663751254 26.792844109587193 21.885120622672886 47 19.157560210340623 26.389534139953273 34.21738340032723 20.235522249378874 48 17.67450629212424 33.7963492886432 28.30950922104243 20.21963519819013 49 17.992068461699017 26.391554056328737 34.952632960995416 20.66374452097683 50 25.472663614328038 27.37947751144627 26.192431995690818 20.955426878534876 51 12.181442354953171 24.95337359699975 20.424047777755334 42.44113627029174 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11.0 1 17.5 2 24.0 3 26.0 4 28.0 5 23.5 6 19.0 7 22.0 8 25.0 9 30.0 10 35.0 11 50.0 12 65.0 13 76.5 14 88.0 15 135.0 16 182.0 17 222.0 18 262.0 19 352.5 20 443.0 21 581.5 22 720.0 23 923.5 24 1127.0 25 1506.0 26 2386.0 27 2887.0 28 3512.0 29 4137.0 30 4886.0 31 5635.0 32 6566.0 33 7497.0 34 8439.5 35 9382.0 36 9999.0 37 10616.0 38 11222.0 39 11828.0 40 11970.5 41 12113.0 42 12060.5 43 12008.0 44 11574.0 45 11140.0 46 10366.5 47 9593.0 48 9955.5 49 10318.0 50 10226.5 51 10135.0 52 10350.5 53 10566.0 54 7532.5 55 4499.0 56 4279.0 57 4059.0 58 3411.5 59 2764.0 60 1999.5 61 1235.0 62 1083.0 63 931.0 64 886.0 65 841.0 66 646.5 67 452.0 68 368.0 69 284.0 70 248.0 71 212.0 72 193.0 73 174.0 74 143.0 75 99.0 76 86.0 77 63.5 78 41.0 79 40.5 80 40.0 81 24.5 82 9.0 83 8.5 84 8.0 85 5.5 86 3.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0686771567657099 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 6.733054584873519E-4 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.004039832750924112 24 0.0 25 0.004713138209411463 26 0.004039832750924112 27 0.0 28 0.0 29 0.0 30 0.002019916375462056 31 6.733054584873519E-4 32 0.0 33 0.0 34 6.733054584873519E-4 35 0.0 36 0.0 37 6.733054584873519E-4 38 0.0 39 0.0 40 6.733054584873519E-4 41 0.00336652729243676 42 0.00336652729243676 43 0.004713138209411463 44 0.008079665501848224 45 6.733054584873519E-4 46 0.0 47 0.0 48 0.0013466109169747039 49 0.0 50 6.733054584873519E-4 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 148521.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.46610939190201 #Duplication Level Percentage of deduplicated Percentage of total 1 96.87275750974949 86.66828720462443 2 2.0408581970368105 3.651752854189103 3 0.5504145760206832 1.477303520074914 4 0.21942534097842906 0.7852452623732612 5 0.0739528653020171 0.3308137568477427 6 0.04559894480112485 0.24477361102396422 7 0.026769852015707085 0.1676496156189595 8 0.028067968613964763 0.2008905560340355 9 0.02378839715219351 0.1915429807688539 >10 0.08621152370585318 1.4651949939114204 >50 0.013641927582185359 0.8559351749303808 >100 0.017032268269946624 3.225423638759592 >500 0.0014806287715850705 0.7351868308432206 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG 585 0.39388369321510086 TruSeq Adapter, Index 15 (97% over 40bp) GATCGGAAGAGCACACGTCTGAACTCCCAGTCACACAGTGATCTCGTATGC 525 0.35348536570585976 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp) ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA 395 0.26595565610250405 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC 394 0.2652823506440167 TruSeq Adapter, Index 5 (100% over 51bp) GATCGGAAGAGCACACGTCCTGAACTCCAGTCACACAGTGATCTCGTATGC 330 0.22219080130082614 TruSeq Adapter, Index 5 (100% over 32bp) CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTC 282 0.18987213929343325 No Hit ACTCTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGA 267 0.17977255741612297 No Hit GCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCC 250 0.168326364621838 No Hit ACCATATCTTCATAACCTGTCACCTTGAAACTACCTCTGCATGCCACCTAC 248 0.1669797537048633 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGTGATCTCGTATGC 245 0.16495983732940123 TruSeq Adapter, Index 7 (97% over 37bp) TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC 226 0.15216703361814155 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCCTCGTATGC 224 0.15082042270116683 TruSeq Adapter, Index 5 (97% over 42bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGATCTCGTATGC 214 0.14408736811629333 TruSeq Adapter, Index 16 (97% over 39bp) AAACAATCAAAATCAGCCATTTCCGCACCTTTTCCTCTGTCCACTTTCAAC 205 0.13802761898990715 No Hit CCAAGCAGTCCACAAGCACGCCCGCTGCGTCTGACCAAGGCCCTCACTACC 188 0.12658142619562218 No Hit ATGGTCTCAATACTGCCGCCGAAATTCTGTCCCACATACTAAATCTCTTCC 181 0.1218682879862107 No Hit GATCGGAAGAGCACACCGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 174 0.11715514977679925 TruSeq Adapter, Index 5 (97% over 36bp) AATCAAAATCAGCCATTTCCGCACCTTTTCCTCTGTCCACTTTCAACCGTC 164 0.11042209519192571 No Hit TTTCCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCC 151 0.10166912423159014 No Hit GATCGGAAGAGCACCACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC 149 0.10032251331461545 TruSeq Adapter, Index 5 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.01750594192067115 0.0 0.0 2 0.01750594192067115 0.0 0.0 3 0.01750594192067115 0.0 0.0 4 0.01750594192067115 0.0 0.0 5 0.01750594192067115 0.0 0.0 6 0.01750594192067115 0.0 0.0 7 0.01750594192067115 0.0 0.0 8 0.018852552837645854 0.0 0.0 9 0.018852552837645854 0.0 0.0 10 0.018852552837645854 0.0 0.0 11 0.018852552837645854 0.0 0.0 12 0.018852552837645854 0.0 0.0 13 0.018852552837645854 0.0 0.0 14 0.018852552837645854 0.0 0.0 15 0.018852552837645854 0.0 0.0 16 0.018852552837645854 0.0 0.0 17 0.02423899650554467 0.0 0.0 18 0.02423899650554467 0.0 0.0 19 0.02423899650554467 0.0 0.0 20 0.02423899650554467 0.0 0.0 21 0.02423899650554467 0.0 0.0 22 0.02423899650554467 0.0 0.0 23 0.02423899650554467 0.0 0.0 24 0.025585607422519373 0.0 0.0 25 0.025585607422519373 0.0 0.0 26 0.026258912881006725 0.0 0.0 27 0.026258912881006725 0.0 0.0 28 0.026258912881006725 0.0 0.0 29 0.02760552379798143 0.0 0.0 30 0.02760552379798143 0.0 0.0 31 0.02760552379798143 0.0 0.0 32 0.02760552379798143 0.0 0.0 33 0.028278829256468782 0.0 0.0 34 0.028278829256468782 0.0 0.0 35 0.028952134714956133 0.0 0.0 36 0.04241824388470317 0.0 0.0 37 0.04241824388470317 0.0 6.733054584873519E-4 38 0.04241824388470317 0.0 6.733054584873519E-4 39 0.04241824388470317 0.0 6.733054584873519E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCCGC 25 3.8681974E-5 45.012123 21 CTACGCA 20 7.015765E-4 44.99697 41 CGCAGTG 20 7.015765E-4 44.99697 44 GCGGCAT 20 7.015765E-4 44.99697 3 CACACGC 20 7.015765E-4 44.99697 12 GATCGGA 1120 0.0 44.223236 1 GTCCCGA 150 0.0 43.49707 35 ATCGGAA 1125 0.0 43.39708 2 TCGGAAG 1130 0.0 43.005955 3 CGATCTC 190 0.0 42.628708 39 CGTATGC 1115 0.0 41.97027 45 CGGAAGA 1145 0.0 41.46009 4 GATCTCG 520 0.0 41.103004 40 GAAGAGC 1125 0.0 40.797253 6 CTCGTAT 1120 0.0 40.778503 43 AGAGCAC 1065 0.0 40.7719 8 TCCCGAT 155 0.0 40.642426 36 TCGTATG 1155 0.0 40.516754 44 TCTCGTA 1075 0.0 40.392628 42 ATCTCGT 1070 0.0 40.16085 41 >>END_MODULE