FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8441a-062415.3410000000d850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8441a-062415.3410000000d850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148521
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG5850.39388369321510086TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACACAGTGATCTCGTATGC5250.35348536570585976Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA3950.26595565610250405No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC3940.2652823506440167TruSeq Adapter, Index 5 (100% over 51bp)
GATCGGAAGAGCACACGTCCTGAACTCCAGTCACACAGTGATCTCGTATGC3300.22219080130082614TruSeq Adapter, Index 5 (100% over 32bp)
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTC2820.18987213929343325No Hit
ACTCTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCCTCTATGA2670.17977255741612297No Hit
GCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCC2500.168326364621838No Hit
ACCATATCTTCATAACCTGTCACCTTGAAACTACCTCTGCATGCCACCTAC2480.1669797537048633No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGTGATCTCGTATGC2450.16495983732940123TruSeq Adapter, Index 7 (97% over 37bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC2260.15216703361814155No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCCTCGTATGC2240.15082042270116683TruSeq Adapter, Index 5 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCGATCTCGTATGC2140.14408736811629333TruSeq Adapter, Index 16 (97% over 39bp)
AAACAATCAAAATCAGCCATTTCCGCACCTTTTCCTCTGTCCACTTTCAAC2050.13802761898990715No Hit
CCAAGCAGTCCACAAGCACGCCCGCTGCGTCTGACCAAGGCCCTCACTACC1880.12658142619562218No Hit
ATGGTCTCAATACTGCCGCCGAAATTCTGTCCCACATACTAAATCTCTTCC1810.1218682879862107No Hit
GATCGGAAGAGCACACCGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1740.11715514977679925TruSeq Adapter, Index 5 (97% over 36bp)
AATCAAAATCAGCCATTTCCGCACCTTTTCCTCTGTCCACTTTCAACCGTC1640.11042209519192571No Hit
TTTCCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCC1510.10166912423159014No Hit
GATCGGAAGAGCACCACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1490.10032251331461545TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC253.8681974E-545.01212321
CTACGCA207.015765E-444.9969741
CGCAGTG207.015765E-444.9969744
GCGGCAT207.015765E-444.996973
CACACGC207.015765E-444.9969712
GATCGGA11200.044.2232361
GTCCCGA1500.043.4970735
ATCGGAA11250.043.397082
TCGGAAG11300.043.0059553
CGATCTC1900.042.62870839
CGTATGC11150.041.9702745
CGGAAGA11450.041.460094
GATCTCG5200.041.10300440
GAAGAGC11250.040.7972536
CTCGTAT11200.040.77850343
AGAGCAC10650.040.77198
TCCCGAT1550.040.64242636
TCGTATG11550.040.51675444
TCTCGTA10750.040.39262842
ATCTCGT10700.040.1608541
Produced by FastQC (version 0.11.2)