FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8440d-062415.3410000000d645.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8440d-062415.3410000000d645.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740435
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC108631.4671105498794628TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA89661.210909803021197No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC83701.1304165794431653No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG70590.9533584987203467TruSeq Adapter, Index 15 (97% over 40bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC27360.36951251629109916No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG12510.1689547360673118TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG11540.15585432887424286TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG10010.13519080000270112TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC7700.10399292307900086TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG28050.044.43336511
GATCGGA28500.043.922291
CGTCTGA28400.043.88577316
CACACGT28600.043.81486512
GTCACAC12000.043.68540229
ACACGTC28700.043.34864813
GAGCACA28750.043.2791069
AGCACAC28850.043.2070810
CACGTCT28750.043.19500714
GAACTCC28800.043.12584321
ACGTCTG28950.043.0520215
TAAGCCG9250.043.0490816
TCCAGTC29000.042.98069425
ATCGGAA29150.042.910992
AGAGCAC29050.042.832168
TCGGAAG29100.042.83013
CTCCAGT29150.042.68234324
CCAGTCA29300.042.61740526
CAGTCAC29300.042.5406227
CTGAACT29150.042.53084619
Produced by FastQC (version 0.11.2)