##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C6001ACXX l05n01 ah8440c-062415.3410000000d5f3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 165810 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.565110668837825 33.0 33.0 33.0 33.0 33.0 2 32.561371449249144 33.0 33.0 33.0 33.0 33.0 3 32.58836017128038 33.0 33.0 33.0 33.0 33.0 4 36.40075990591641 37.0 37.0 37.0 37.0 37.0 5 36.451776129304626 37.0 37.0 37.0 37.0 37.0 6 36.367830649538625 37.0 37.0 37.0 37.0 37.0 7 36.332181412460045 37.0 37.0 37.0 37.0 37.0 8 36.303546227609914 37.0 37.0 37.0 37.0 37.0 9 36.31479404137266 37.0 37.0 37.0 37.0 37.0 10 36.28488028466317 37.0 37.0 37.0 37.0 37.0 11 36.30134491285206 37.0 37.0 37.0 37.0 37.0 12 36.26828900548821 37.0 37.0 37.0 33.0 37.0 13 36.253700018093 37.0 37.0 37.0 33.0 37.0 14 38.24560641698329 40.0 37.0 40.0 37.0 40.0 15 38.188492853265785 40.0 37.0 40.0 33.0 40.0 16 38.20969784693324 40.0 37.0 40.0 33.0 40.0 17 38.20458959049515 40.0 37.0 40.0 33.0 40.0 18 38.21013810988481 40.0 37.0 40.0 33.0 40.0 19 38.21703154212653 40.0 37.0 40.0 33.0 40.0 20 38.19019962607804 40.0 37.0 40.0 33.0 40.0 21 38.15620288281768 40.0 37.0 40.0 33.0 40.0 22 38.08640612749532 40.0 37.0 40.0 33.0 40.0 23 38.061811712200715 40.0 37.0 40.0 33.0 40.0 24 38.0872263434051 40.0 37.0 40.0 33.0 40.0 25 38.04383330317834 40.0 37.0 40.0 33.0 40.0 26 37.94912852059586 40.0 37.0 40.0 33.0 40.0 27 37.903051685664316 40.0 37.0 40.0 33.0 40.0 28 37.79526566552077 40.0 37.0 40.0 33.0 40.0 29 37.79360110970388 40.0 37.0 40.0 33.0 40.0 30 37.75739098968699 40.0 37.0 40.0 33.0 40.0 31 37.67229962004704 40.0 37.0 40.0 33.0 40.0 32 37.60571738737109 40.0 37.0 40.0 33.0 40.0 33 37.596085881430554 40.0 37.0 40.0 33.0 40.0 34 37.48040528315542 40.0 37.0 40.0 33.0 40.0 35 37.40405283155419 40.0 37.0 40.0 33.0 40.0 36 37.35894095651649 40.0 37.0 40.0 33.0 40.0 37 37.34453289910138 40.0 37.0 40.0 33.0 40.0 38 37.27115976117243 40.0 37.0 40.0 33.0 40.0 39 37.21258066461613 40.0 37.0 40.0 33.0 40.0 40 37.12726614800072 40.0 37.0 40.0 33.0 40.0 41 37.09442132561365 40.0 37.0 40.0 33.0 40.0 42 37.01986008081539 40.0 37.0 40.0 33.0 40.0 43 36.94845304867017 40.0 37.0 40.0 33.0 40.0 44 36.93201254447862 40.0 37.0 40.0 33.0 40.0 45 36.842403956335566 40.0 37.0 40.0 33.0 40.0 46 36.80025933297147 40.0 37.0 40.0 33.0 40.0 47 36.67336107593028 40.0 37.0 40.0 33.0 40.0 48 36.671678427115374 40.0 37.0 40.0 33.0 40.0 49 36.59131536095531 40.0 37.0 40.0 33.0 40.0 50 36.53510041613895 40.0 37.0 40.0 33.0 40.0 51 35.59523551052409 37.0 37.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1306 1 -0.18604044653041996 1306 2 -0.0893785559849718 1306 3 -0.006568124006349763 1306 4 0.022594349585844498 1306 5 0.08841134641121329 1306 6 -0.012695650974123396 1306 7 0.16576280317894287 1306 8 0.21347608431342024 1306 9 0.014162074781424394 1306 10 0.1213728079694647 1306 11 -0.15069444994030334 1306 12 -0.15425388591207678 1306 13 0.1960892501881517 1306 14 0.25184920002109834 1306 15 0.2464928800838706 1306 16 0.33040166728397224 1306 17 0.3244045629769161 1306 18 0.30349420762261303 1306 19 0.2791161009708105 1306 20 0.37269237473672234 1306 21 0.5746791363101096 1306 22 0.29023844554932055 1306 23 0.5999905958179426 1306 24 0.5657128984922011 1306 25 0.3099419125909222 1306 26 0.4634003220021512 1306 27 0.17818022618313734 1306 28 0.4545046900645815 1306 29 0.6562821381805364 1306 30 0.7806273621253084 1306 31 0.5837660450614095 1306 32 0.8299673607364113 1306 33 0.8745783004475669 1306 34 0.5199045770337634 1306 35 0.6757450391470172 1306 36 0.269839521413644 1306 37 0.3030591531064246 1306 38 0.23003958164199645 1306 39 0.8228946987849213 1306 40 0.6520904599984689 1306 41 0.6652853088054371 1306 42 0.660429121588777 1306 43 0.7420476172281596 1306 44 0.9887474952198474 1306 45 0.6718601219550564 1306 46 0.726284924370546 1306 47 0.6079043988392314 1306 48 0.4248075698420095 1306 49 1.0391337518374044 1306 50 0.9286002926729893 1306 51 -0.19194724546519382 1101 1 0.10872825212932469 1101 2 0.11088084738727133 1101 3 0.05065914217033196 1101 4 0.14413924078054663 1101 5 0.15824849873863656 1101 6 0.03983353190525207 1101 7 0.11970429330720123 1101 8 0.30183175515936256 1101 9 0.2574536153492275 1101 10 -0.1268473966709749 1101 11 0.2623045412688043 1101 12 -0.009377102513894897 1101 13 0.0256035894301192 1101 14 0.3442400344352805 1101 15 0.32040842987490237 1101 16 0.2977600762766244 1101 17 0.46733582108327454 1101 18 0.46142762742373833 1101 19 0.29406061747174306 1101 20 0.26002484339215215 1101 21 0.6063695816192336 1101 22 0.6453040169573043 1101 23 0.36819927141961273 1101 24 0.4501919300529451 1101 25 0.5303627225058989 1101 26 0.5222704762030475 1101 27 0.008573654631035765 1101 28 0.33919989110219007 1101 29 0.3723507359611986 1101 30 0.1559552191652287 1101 31 0.05402112504411605 1101 32 0.47622762878656744 1101 33 0.5963681837160522 1101 34 0.2611785358174501 1101 35 0.598831939276721 1101 36 0.6348200661607208 1101 37 0.4284662722878636 1101 38 0.7253414983446902 1101 39 0.685310032406484 1101 40 0.6209188214338326 1101 41 0.5628065654706589 1101 42 0.37636801768331196 1101 43 0.4788540734898632 1101 44 0.32711037171502255 1101 45 0.5030749944150656 1101 46 0.49303805882796325 1101 47 0.14182859540934345 1101 48 0.2109006668300495 1101 49 0.40255787635102536 1101 50 0.6141025264242543 1101 51 0.778264600622137 1305 1 -0.15622901282793578 1305 2 0.008316744823169131 1305 3 0.24353663504782475 1305 4 0.00852670516800913 1305 5 0.15425989475003377 1305 6 0.3552181472898681 1305 7 -0.06776009415718676 1305 8 0.15624770957531808 1305 9 0.1440633019589157 1305 10 0.3198762500526726 1305 11 0.2731307520950139 1305 12 0.07011007697328608 1305 13 0.11677168059820531 1305 14 0.2476588378540825 1305 15 0.44320045266692887 1305 16 0.25246093097747746 1305 17 -0.2454846917372393 1305 18 -0.18558091958480816 1305 19 0.5587329821441145 1305 20 0.7865205698878768 1305 21 0.06363453888419457 1305 22 0.46467723633052316 1305 23 0.49355539677574 1305 24 0.47412355398456896 1305 25 0.67252796467114 1305 26 0.1630112169437865 1305 27 0.7017360477934318 1305 28 0.07509732699963223 1305 29 0.20539916900963107 1305 30 0.7143597775697828 1305 31 1.0890638600868527 1305 32 1.144603697162637 1305 33 0.40406049140835876 1305 34 0.3617483363872509 1305 35 0.9244729649177472 1305 36 1.157896989237642 1305 37 1.5538223976439909 1305 38 0.5170793900825856 1305 39 1.2269054740019243 1305 40 1.4200641205791342 1305 41 0.6907267933908869 1305 42 1.4991600404753385 1305 43 0.660335554971347 1305 44 0.6778226224272714 1305 45 1.0124767038167732 1305 46 0.5434654092553117 1305 47 0.8047915583723011 1305 48 1.0436641995935787 1305 49 1.4794809532740985 1305 50 0.9813389936607209 1305 51 1.7893757117332498 1102 1 0.3976171410182161 1102 2 0.37754751405393705 1102 3 0.3358443273555167 1102 4 0.47006516670646903 1102 5 -0.10947636898622903 1102 6 0.5398335319052521 1102 7 0.593778367381276 1102 8 0.5870169403445473 1102 9 0.6056017634973756 1102 10 0.6583377885142099 1102 11 0.5808230597873205 1102 12 0.623956230819438 1102 13 0.6552332190597454 1102 14 1.555351145546389 1102 15 0.6278158372823128 1102 16 1.1469664254829723 1102 17 1.1720977258451768 1102 18 1.1550784210745277 1102 19 1.512579135990265 1102 20 1.5711359545032622 1102 21 1.1889092641589158 1102 22 1.3130288846821685 1102 23 0.8517971550175005 1102 24 0.7708268506878682 1102 25 1.3054949976381707 1102 26 1.3344397883723573 1102 27 0.600637146694531 1102 28 1.048723700626006 1102 29 1.9130914767019362 1102 30 -0.8197061564961459 1102 31 1.6143385853615726 1102 32 1.6698784224373568 1102 33 2.096368183716052 1102 34 2.1463637210026363 1102 35 2.232165272610054 1102 36 2.2348200661607223 1102 37 2.369207013028607 1102 38 2.4401563131595054 1102 39 2.5038285509250002 1102 40 2.1145696150846263 1102 41 2.108309210973303 1102 42 2.6837754250907224 1102 43 2.660335554971347 1102 44 2.708591853196502 1102 45 2.85863054997062 1102 46 2.024784090573995 1102 47 -0.42377987019912666 1102 48 0.3447631006924823 1102 49 -0.08315640936326218 1102 50 1.8802400925618201 1102 51 3.3322328545903943 1304 1 -0.13179462368766792 1304 2 -0.08883904056791181 1304 3 -0.01289516844280314 1304 4 0.07720802384932313 1304 5 -0.07796376394421145 1304 6 0.05243857392206053 1304 7 0.018148115280432364 1304 8 -0.08315112688234194 1304 9 -0.03935621969590386 1304 10 0.028085687673872428 1304 11 0.07031885810664562 1304 12 -0.10503536581921935 1304 13 -0.1346827473268064 1304 14 -0.2870858292435301 1304 15 -0.22932701986054127 1304 16 -0.1009327341808941 1304 17 0.052349826685514245 1304 18 -0.21887115875740193 1304 19 -0.3466645614887298 1304 20 -0.38894807911018603 1304 21 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6 -0.18488556921834487 1209 7 0.02636263704419406 1209 8 -0.24257107463672867 1209 9 -0.11537201927416163 1209 10 -0.1712502264670661 1209 11 -0.2262930450815901 1209 12 -0.35918983659629333 1209 13 -0.15001022663313535 1209 14 -0.4277949218693422 1209 15 -0.6362291065379111 1209 16 -0.5892561534362315 1209 17 -0.38060425382309404 1209 18 -0.4107321728259521 1209 19 -0.6709414632606681 1209 20 -0.6386018732120959 1209 21 -0.5098601312396909 1209 22 -0.5786244058475276 1209 23 -0.45023601780219025 1209 24 -0.5630415281243373 1209 25 -0.6385927498203614 1209 26 -0.5778127530936672 1209 27 -0.4881804027971768 1209 28 -0.4948825059015505 1209 29 -0.49702088284862356 1209 30 -0.3239865203270753 1209 31 -0.6069027201493924 1209 32 -0.3622243062945856 1209 33 -0.4186130896921867 1209 34 -0.20569620409099798 1209 35 -0.039370307914289526 1209 36 -0.25581663795912846 1209 37 -0.5783585300425571 1209 38 -0.3744504284135317 1209 39 -0.5261339958914775 1209 40 -0.801428244733458 1209 41 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1215 10 -0.5336527886353792 1215 11 -0.40622052089583605 1215 12 -0.35484235575300005 1215 13 -0.5014216690439071 1215 14 -0.7932943197068525 1215 15 -0.6819603700203984 1215 16 -0.8072654439432441 1215 17 -0.6690599386340423 1215 18 -0.641320703946505 1215 19 -0.7653949511711033 1215 20 -0.7621973788300735 1215 21 -0.7346983414274888 1215 22 -0.6730051048853909 1215 23 -0.8682095755899368 1215 24 -0.8125485489419546 1215 25 -0.7936131968763789 1215 26 -0.5809228231033288 1215 27 -0.3798440917372403 1215 28 -0.6017895081910893 1215 29 -0.6982159437927606 1215 30 -0.44666223928262383 1215 31 -0.6037330956076303 1215 32 -0.829700914597808 1215 33 -0.4701806973204583 1215 34 -0.5344372212824027 1215 35 -0.660649803950605 1215 36 -0.6850857053351547 1215 37 -0.9688377455108537 1215 38 -0.9732712486779533 1215 39 -0.6375142052587464 1215 40 -0.7560344569328734 1215 41 -0.7505162980288844 1215 42 -0.8003235148386096 1215 43 -0.9062133260651635 1215 44 -0.9180276992181007 1215 45 -0.8103918292991068 1215 46 -0.9272603314350931 1215 47 -0.5523344722519568 1215 48 -0.46745295180625135 1215 49 -0.45704165250645445 1215 50 -0.5242694144211839 1215 51 -0.328881060940482 1212 1 -0.17851139761988577 1212 2 -0.22076924187567215 1212 3 -0.27318398174930536 1212 4 -0.573546156935457 1212 5 -0.3651327248721188 1212 6 -0.38518559586827195 1212 7 -0.21723923017037094 1212 8 -0.2408330979110005 1212 9 -0.3966935693258833 1212 10 -0.2758626705523568 1212 11 -0.26691986293647574 1212 12 -0.38828554423794515 1212 13 -0.3837874389356628 1212 14 -0.6152685943158929 1212 15 -0.5259714618760682 1212 16 -0.5530007840481233 1212 17 -0.44753283487183637 1212 18 -0.511223907048759 1212 19 -0.5141997469477602 1212 20 -0.5000193630177776 1212 21 -0.7130472338190046 1212 22 -0.5774509491794504 1212 23 -0.6177623596835602 1212 24 -0.7836599784737928 1212 25 -0.5788639486947602 1212 26 -0.5652213767823042 1212 27 -0.5237480320135219 1212 28 -0.49199113159609453 1212 29 -0.5061893190134441 1212 30 -0.4981409581138152 1212 31 -0.4230425491886507 1212 32 -0.45625558127889576 1212 33 -0.35734260434821863 1212 34 -0.47414125221848025 1212 35 -0.39369547719866915 1212 36 -0.42776600882015003 1212 37 -0.3614739357089576 1212 38 -0.5606087977815832 1212 39 -0.4747483427245811 1212 40 -0.42319407493614136 1212 41 -0.7423848539518261 1212 42 -0.6658802749857884 1212 43 -0.5707279492367618 1212 44 -0.7833744819220883 1212 45 -0.6976051041992335 1212 46 -0.6513553782204085 1212 47 -0.6723316244892104 1212 48 -0.4834148422521025 1212 49 -0.39269843581423913 1212 50 -0.5065781389388917 1212 51 -0.2161330870425715 1213 1 0.20354706015568524 1213 2 0.1430461663450444 1213 3 0.04473920606171333 1213 4 0.15470128530485283 1213 5 -0.2792876897409471 1213 6 -0.2391422093346378 1213 7 0.19485653449717688 1213 8 0.21504928535802748 1213 9 0.21476618398254743 1213 10 0.0761275459266102 1213 11 0.09025702205147468 1213 12 0.327460273946123 1213 13 0.06763214089262704 1213 14 0.4475344339560934 1213 15 0.3771419828348712 1213 16 0.39224944435090237 1213 17 0.3580815533384438 1213 18 0.22515928360822102 1213 19 0.25651444596331174 1213 20 0.21264538846552483 1213 21 0.013707107824686204 1213 22 -0.15866922852537613 1213 23 -0.20750203635716247 1213 24 -0.30734026521510316 1213 25 -0.44652656570414706 1213 26 -0.660169376048124 1213 27 -0.5260474894240659 1213 28 -0.7733787252500051 1213 29 -1.4966120273411931 1213 30 -1.286013434124179 1213 31 -1.822319096579129 1213 32 -1.9662401759453871 1213 33 -2.4265428674968845 1213 34 -2.584094500021621 1213 35 -2.6276730023225596 1213 36 -2.7031853246748554 1213 37 -3.0431919088042747 1213 38 -3.0746684846841603 1213 39 -3.202370909991444 1213 40 -2.8261311935407107 1213 41 -3.5682406542558027 1213 42 -3.801399777065612 1213 43 -3.7062412644356613 1213 44 -4.50434615219433 1213 45 -3.877218506633156 1213 46 -3.7407145617171125 1213 47 -3.9817313526789064 1213 48 -3.930169513862772 1213 49 -4.0858518271530215 1213 50 -4.704665567815539 1213 51 -4.543777927080768 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 12.0 10 15.0 11 15.0 12 9.0 13 7.0 14 4.0 15 2.0 16 2.0 17 6.0 18 14.0 19 23.0 20 63.0 21 106.0 22 189.0 23 309.0 24 463.0 25 649.0 26 831.0 27 1074.0 28 1433.0 29 1820.0 30 2333.0 31 2993.0 32 3894.0 33 4937.0 34 6311.0 35 8689.0 36 12967.0 37 23059.0 38 86124.0 39 7454.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 16.425458978599107 25.984042874213912 31.949255857182518 25.641242290004467 2 18.131596405524395 31.207406067185335 29.191242988963275 21.469754538327003 3 17.396417586394065 27.286050298534466 34.59682769434895 20.720704420722512 4 17.38676798745552 26.556902478740728 32.70249080272601 23.35383873107774 5 21.036125686026175 27.561666968216635 32.40275013569748 18.999457210059706 6 21.469754538327003 27.523671672396116 29.23587238405404 21.770701405222844 7 18.93070381762258 30.19540437850552 30.396839756347628 20.477052047524275 8 18.49707496532175 30.64471382908148 30.37331885893493 20.484892346661844 9 20.97219709305832 27.045413425004522 30.98244979193052 20.999939690006634 10 19.158534673051637 29.809536446208217 29.63463324608277 21.39729563465737 11 20.616368132199504 27.54176467040589 29.955973704842894 21.885893492551716 12 19.518726252940112 27.372293589047704 29.397503166274653 23.71147699173753 13 18.022435317532114 29.571195947168444 31.058440383571558 21.347928351727884 14 19.409565164947832 26.93263373741029 30.5355527410892 23.12224835655268 15 19.20933598697304 30.319039864905616 29.560340148362585 20.91128399975876 16 18.1388336047283 27.991074120981846 30.452928050177913 23.417164224111936 17 20.481273747059888 27.746818647849945 30.597672034256075 21.17423557083409 18 18.80767143115614 27.372293589047704 32.40636873529944 21.413666244496714 19 18.982570411917255 28.402388275737287 29.517520053072793 23.09752125927266 20 18.731680839515107 27.628007960919128 31.919667088836622 21.720644110729147 21 21.95223448525421 27.034557626198662 29.171943791086186 21.84126409746095 22 18.849888426512273 29.691212833966592 29.95235510524094 21.506543634280202 23 18.293558173056628 30.647077626396957 29.78402600613967 21.27533819440675 24 18.459079669501236 27.528496471865388 29.402327965743925 24.610095892889454 25 18.795233040625302 26.946226961304642 32.51531892309177 21.74322107497829 26 18.87544629933417 27.727250796101515 30.17827849078452 23.219024413779792 27 19.61919581199829 27.46868346933483 28.23161868919889 24.680502029467995 28 18.474157167842712 29.53199445148061 30.148965683613778 21.844882697062904 29 21.068089982510102 27.08642422049334 29.63994933960557 22.20553645739099 30 19.396296966407334 27.67866835534648 30.919727398829988 22.0053072794162 31 18.51577106326518 27.669621856341596 30.776189614619142 23.038417465774078 32 18.79862493215126 29.745491827995902 30.997527290271996 20.458355949580845 33 18.594174054640856 27.28001929919788 30.0 24.12580664616127 34 19.162294192147638 29.158072492612025 30.384174657740786 21.295458657499548 35 18.509740063928593 27.544177070140524 30.760509016344006 23.185573849586877 36 19.225619685181837 29.42223026355467 29.06881370243049 22.283336348833004 37 20.397442856281288 27.950666425426697 30.82504070924552 20.8268500090465 38 18.731680839515107 28.83963572764007 31.84729509679754 20.581388336047283 39 20.408298655087147 28.113503407514624 29.085097400639288 22.393100536758944 40 18.82889348587833 29.863276420459684 30.36867721293778 20.939152880724208 41 18.737447755522986 27.65806027489792 32.382227529597664 21.222264439981426 42 19.97828774765538 26.88338711136576 29.21564488405054 23.92268025692832 43 19.61374451889962 27.276126829797885 32.80879629908864 20.30133235221385 44 18.08339967672674 27.594629803864805 30.42990518925961 23.892065330148853 45 20.885728588057198 26.78234332688005 29.85338375340004 22.478544331662718 46 18.477172667511006 26.46945298836017 33.139738254628796 21.91363608950003 47 19.516916953139134 29.747904227730537 30.1941981786382 20.54098064049213 48 18.82297597220882 27.049358293930332 33.05630608897038 21.071359644890475 49 19.74609492792956 27.634038960255715 31.162776672094566 21.45708943972016 50 18.23889508760291 27.09749404420856 30.66493772805404 23.998673140134496 51 11.139993245199268 24.269637170703465 20.00325677892502 44.58711280517225 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 53.0 1 60.5 2 68.0 3 76.5 4 85.0 5 79.0 6 73.0 7 66.0 8 59.0 9 77.0 10 95.0 11 107.0 12 119.0 13 154.5 14 190.0 15 253.5 16 317.0 17 370.5 18 424.0 19 560.0 20 696.0 21 872.5 22 1049.0 23 1393.5 24 1738.0 25 2222.5 26 3426.5 27 4146.0 28 4849.5 29 5553.0 30 6588.0 31 7623.0 32 8685.5 33 9748.0 34 10768.0 35 11788.0 36 12582.0 37 13376.0 38 13619.0 39 13862.0 40 14093.5 41 14325.0 42 14083.5 43 13842.0 44 13494.0 45 13146.0 46 11854.5 47 10563.0 48 10579.5 49 10596.0 50 9553.0 51 8510.0 52 8460.0 53 8410.0 54 6170.0 55 3930.0 56 3361.0 57 2792.0 58 2644.0 59 2496.0 60 1855.0 61 1214.0 62 1016.5 63 819.0 64 769.5 65 720.0 66 501.0 67 282.0 68 228.0 69 174.0 70 127.5 71 81.0 72 73.5 73 66.0 74 55.5 75 28.5 76 12.0 77 9.5 78 7.0 79 6.5 80 6.0 81 3.5 82 1.0 83 1.0 84 1.0 85 1.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06995959230444485 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0024123997346360295 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.001809299800977022 24 0.0 25 0.0012061998673180147 26 0.0012061998673180147 27 0.001809299800977022 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 6.030999336590074E-4 41 0.001809299800977022 42 0.0030154996682950365 43 0.007840299137567095 44 0.003618599601954044 45 0.001809299800977022 46 0.0 47 0.0 48 0.0012061998673180147 49 0.0 50 0.0030154996682950365 51 0.0012061998673180147 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 165810.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.94374997198389 #Duplication Level Percentage of deduplicated Percentage of total 1 96.1611014777685 84.56767865391443 2 2.6563366019451755 4.672164039257916 3 0.6121427601065664 1.6150238952591596 4 0.20499990858580136 0.7211384281979707 5 0.1092203845298027 0.48026250944664534 6 0.0883118811282249 0.46598867960977036 7 0.042139133777122445 0.2594111411451866 8 0.03382316210819143 0.23796285693637337 9 0.018127409176338413 0.14347731062193764 >10 0.05155496082922486 0.8247596071771661 >50 0.0028579566740429435 0.1740483257612173 >100 0.01592505532144829 2.716132505890664 >500 0.002075584829804941 1.167005267146385 >1k 0.0013837232198694541 1.9549467796351552 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC 2188 1.319582654845908 TruSeq Adapter, Index 5 (100% over 51bp) CATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGC 1025 0.6181774320004825 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC 808 0.4873047463964779 TruSeq Adapter, Index 12 (100% over 51bp) TGTCTTCAACTGCTTTCGCATGAAGTACCTCCCAACTACTTTTCCTCACAC 595 0.35884446052710933 No Hit CTCTGCATGCCACCTACCGACCAACTTTCATGTTCTGTTTCGACCTACCTC 515 0.31059646583438877 No Hit ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA 433 0.2611422712743502 No Hit TCACTACCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCC 303 0.1827392798986792 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG 291 0.17550208069477113 TruSeq Adapter, Index 15 (97% over 40bp) ACTTGTACTCCATGACTAAACCCCCCCTCCCATTACAAACTAAAATCTTAC 282 0.17007418129184004 No Hit AGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTC 267 0.16102768228695497 No Hit TAAAAAGCTCGTAGTTGAACTTTGGGCCCGGTTGGCCGGTCCGATTTTTTC 217 0.13087268560400458 No Hit GCCTGCCACCATCCATTTGTCTTTTGCACCATATCTTCATAACCTGTCACC 217 0.13087268560400458 No Hit CATAGGTAATCTTGCACGTCGCATCCCCGGTTCATTTTCTGCGTTTCCATC 205 0.1236354864000965 No Hit TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC 202 0.12182618659911948 No Hit GCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATTTACA 196 0.11820758699716542 No Hit TGGCTATTCATCTTGCACTTTTCCTCTTTCTTCTTCCCAGTAGCCTCATCC 191 0.1151920873288704 No Hit TCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAAC 189 0.11398588746155237 No Hit TATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCC 185 0.11157348772691635 No Hit ACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCC 168 0.10132078885471323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.030999336590073E-4 0.0 0.0 2 6.030999336590073E-4 0.0 0.0 3 6.030999336590073E-4 0.0 0.0 4 6.030999336590073E-4 0.0 0.0 5 6.030999336590073E-4 0.0 0.0 6 0.0012061998673180145 0.0 0.0 7 0.013871298474157167 0.0 0.0 8 0.013871298474157167 0.0 0.0 9 0.019299197877088232 0.0 0.0 10 0.019299197877088232 0.0 0.0 11 0.01990229781074724 0.0 0.0 12 0.01990229781074724 0.0 0.0 13 0.01990229781074724 0.0 0.0 14 0.01990229781074724 0.0 0.0 15 0.01990229781074724 0.0 0.0 16 0.01990229781074724 0.0 0.0 17 0.021108497678065257 0.0 0.0 18 0.021108497678065257 0.0 6.030999336590073E-4 19 0.021108497678065257 0.0 6.030999336590073E-4 20 0.021108497678065257 0.0 6.030999336590073E-4 21 0.021108497678065257 0.0 6.030999336590073E-4 22 0.021108497678065257 0.0 6.030999336590073E-4 23 0.021108497678065257 0.0 6.030999336590073E-4 24 0.021108497678065257 0.0 6.030999336590073E-4 25 0.021711597611724264 0.0 6.030999336590073E-4 26 0.021711597611724264 0.0 6.030999336590073E-4 27 0.021711597611724264 0.0 6.030999336590073E-4 28 0.021711597611724264 0.0 6.030999336590073E-4 29 0.021711597611724264 0.0 6.030999336590073E-4 30 0.021711597611724264 0.0 6.030999336590073E-4 31 0.021711597611724264 0.0 6.030999336590073E-4 32 0.021711597611724264 0.0 6.030999336590073E-4 33 0.02231469754538327 0.0 6.030999336590073E-4 34 0.02231469754538327 0.0 6.030999336590073E-4 35 0.02231469754538327 0.0 6.030999336590073E-4 36 0.022917797479042278 0.0 6.030999336590073E-4 37 0.022917797479042278 0.0 6.030999336590073E-4 38 0.022917797479042278 0.0 6.030999336590073E-4 39 0.023520897412701285 0.0 6.030999336590073E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCGGG 20 7.018131E-4 44.996986 15 GCGACCA 30 2.1552314E-6 44.996986 13 CGCGCCT 40 6.7593646E-9 44.996986 35 GCCCGGC 20 7.018131E-4 44.996986 30 CCCCGGT 25 3.877865E-5 44.996983 25 GATCGGA 435 0.0 42.941105 1 GAGCACA 430 0.0 42.91704 9 CACACCG 100 0.0 42.747135 37 ACCGCCC 95 0.0 42.628723 40 CCGCCCG 95 0.0 42.628723 41 AGAGCAC 435 0.0 42.423744 8 AGCACAC 440 0.0 41.941654 10 GTCACAC 280 0.0 41.782913 29 TCGGAAG 450 0.0 41.49722 3 ACACGTC 450 0.0 40.997253 13 ACGTCTG 450 0.0 40.997253 15 CACCGCC 105 0.0 40.71156 39 ATCGGAA 460 0.0 40.595108 2 GCACACG 450 0.0 40.497288 11 ACTCCAG 450 0.0 40.497288 23 >>END_MODULE