FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8440c-062415.3410000000d5f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8440c-062415.3410000000d5f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165810
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC21881.319582654845908TruSeq Adapter, Index 5 (100% over 51bp)
CATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGC10250.6181774320004825No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC8080.4873047463964779TruSeq Adapter, Index 12 (100% over 51bp)
TGTCTTCAACTGCTTTCGCATGAAGTACCTCCCAACTACTTTTCCTCACAC5950.35884446052710933No Hit
CTCTGCATGCCACCTACCGACCAACTTTCATGTTCTGTTTCGACCTACCTC5150.31059646583438877No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA4330.2611422712743502No Hit
TCACTACCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCC3030.1827392798986792No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG2910.17550208069477113TruSeq Adapter, Index 15 (97% over 40bp)
ACTTGTACTCCATGACTAAACCCCCCCTCCCATTACAAACTAAAATCTTAC2820.17007418129184004No Hit
AGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTGATTTC2670.16102768228695497No Hit
TAAAAAGCTCGTAGTTGAACTTTGGGCCCGGTTGGCCGGTCCGATTTTTTC2170.13087268560400458No Hit
GCCTGCCACCATCCATTTGTCTTTTGCACCATATCTTCATAACCTGTCACC2170.13087268560400458No Hit
CATAGGTAATCTTGCACGTCGCATCCCCGGTTCATTTTCTGCGTTTCCATC2050.1236354864000965No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC2020.12182618659911948No Hit
GCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTATTTACA1960.11820758699716542No Hit
TGGCTATTCATCTTGCACTTTTCCTCTTTCTTCTTCCCAGTAGCCTCATCC1910.1151920873288704No Hit
TCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAAC1890.11398588746155237No Hit
TATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCC1850.11157348772691635No Hit
ACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCC1680.10132078885471323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGG207.018131E-444.99698615
GCGACCA302.1552314E-644.99698613
CGCGCCT406.7593646E-944.99698635
GCCCGGC207.018131E-444.99698630
CCCCGGT253.877865E-544.99698325
GATCGGA4350.042.9411051
GAGCACA4300.042.917049
CACACCG1000.042.74713537
ACCGCCC950.042.62872340
CCGCCCG950.042.62872341
AGAGCAC4350.042.4237448
AGCACAC4400.041.94165410
GTCACAC2800.041.78291329
TCGGAAG4500.041.497223
ACACGTC4500.040.99725313
ACGTCTG4500.040.99725315
CACCGCC1050.040.7115639
ATCGGAA4600.040.5951082
GCACACG4500.040.49728811
ACTCCAG4500.040.49728823
Produced by FastQC (version 0.11.2)