FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8440a-062415.3410000000d596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8440a-062415.3410000000d596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691559
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG47310.6841064898295012TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC20830.3012035126431729TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA19900.28775563617854727No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC8060.11654826269342168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT9500.041.4465712
GCACACG9600.041.24920711
GAGCACA9850.040.4306959
GATCGGA10250.040.424541
ACACGTC9800.040.40738713
CACGTCT9850.040.20227414
TCGGAAG10500.040.0706563
AGCACAC9950.040.02435310
ACGTCTG10100.039.4299315
CGTCTGA10050.039.4022316
ATCGGAA10750.038.720182
GAACTCC9900.038.63561621
AGAGCAC10450.038.3246238
CTGACCC2650.038.2068140
TAAGCCG2600.038.07618716
TCCAGTC9600.037.96801825
GTCTGAA10400.037.85984417
CAGTCAC9700.037.576627
AGTCACA8550.036.3150928
ACTCCAG10200.036.1757723
Produced by FastQC (version 0.11.2)