FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8383b-062415.3410000000d8e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8383b-062415.3410000000d8e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347046
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC36431.0497167522461TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA34981.0079355474490415No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC12250.35297914397515023No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG11230.3235882274972194TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC9510.2740270742207085TruSeq Adapter, Index 12 (100% over 51bp)
CCCATAACACCTCTCACTCCCACCTACTGAACATGTCTGGACCCTGCCCTC5830.16798925790817357No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC3580.10315635391273778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGA302.1625838E-644.99553314
ACACGTC8450.043.66430713
GATCGGA8650.043.472581
GTCACAC4250.043.40745529
ACGTCTG8650.042.91481815
GAGCACA8650.042.9148189
CACACGT8600.042.90271812
CACGTCT8700.042.6681814
AGCACAC8650.042.65472810
CCAGGCG1900.042.63348835
CCAGGGA4500.042.495785
GAACTCC8550.042.37642721
CAGTCAC8550.042.36421627
CACACAG4250.042.3487431
GCACACG8750.042.1672411
CCCTGAC4650.042.09866338
CGTCATA4650.042.09259827
TAAGCCG4600.042.0610416
CGTCTGA8800.041.92765416
TGACCCT4700.041.65080641
Produced by FastQC (version 0.11.2)