##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C6001ACXX l05n01 ah8115b-062415.3410000000d737.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 328010 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.58414682479193 33.0 33.0 33.0 33.0 33.0 2 32.576323892564254 33.0 33.0 33.0 33.0 33.0 3 32.61657876284259 33.0 33.0 33.0 33.0 33.0 4 36.47638486631505 37.0 37.0 37.0 37.0 37.0 5 36.48144263894393 37.0 37.0 37.0 37.0 37.0 6 36.384171214292245 37.0 37.0 37.0 37.0 37.0 7 36.3433980671321 37.0 37.0 37.0 37.0 37.0 8 36.32445657144599 37.0 37.0 37.0 37.0 37.0 9 36.33918782963934 37.0 37.0 37.0 37.0 37.0 10 36.31096003170635 37.0 37.0 37.0 37.0 37.0 11 36.31824639492699 37.0 37.0 37.0 37.0 37.0 12 36.29874089204598 37.0 37.0 37.0 37.0 37.0 13 36.2963202341392 37.0 37.0 37.0 37.0 37.0 14 38.30067680863388 40.0 37.0 40.0 37.0 40.0 15 38.25699216487302 40.0 37.0 40.0 37.0 40.0 16 38.26388829608853 40.0 37.0 40.0 37.0 40.0 17 38.26309868601567 40.0 37.0 40.0 37.0 40.0 18 38.22819121368251 40.0 37.0 40.0 37.0 40.0 19 38.27720191457578 40.0 37.0 40.0 37.0 40.0 20 38.24139507941831 40.0 37.0 40.0 37.0 40.0 21 38.2088229017408 40.0 37.0 40.0 33.0 40.0 22 38.157165940062804 40.0 37.0 40.0 33.0 40.0 23 38.109789335690984 40.0 37.0 40.0 33.0 40.0 24 38.154730038718334 40.0 37.0 40.0 33.0 40.0 25 38.134291637450076 40.0 37.0 40.0 33.0 40.0 26 38.07791530746014 40.0 37.0 40.0 33.0 40.0 27 38.04586140666443 40.0 37.0 40.0 33.0 40.0 28 37.98272918508582 40.0 37.0 40.0 33.0 40.0 29 37.974686747355264 40.0 37.0 40.0 33.0 40.0 30 37.91993231913661 40.0 37.0 40.0 33.0 40.0 31 37.892875217218986 40.0 37.0 40.0 33.0 40.0 32 37.85469650315539 40.0 37.0 40.0 33.0 40.0 33 37.83197158623213 40.0 37.0 40.0 33.0 40.0 34 37.74078534191031 40.0 37.0 40.0 33.0 40.0 35 37.70561263376116 40.0 37.0 40.0 33.0 40.0 36 37.67626901618853 40.0 37.0 40.0 33.0 40.0 37 37.64456266577238 40.0 37.0 40.0 33.0 40.0 38 37.60256394622115 40.0 37.0 40.0 33.0 40.0 39 37.542748696686076 40.0 37.0 40.0 33.0 40.0 40 37.520310356391576 40.0 37.0 40.0 33.0 40.0 41 37.48729307033322 40.0 37.0 40.0 33.0 40.0 42 37.403393189232034 40.0 37.0 40.0 33.0 40.0 43 37.35944026096765 40.0 37.0 40.0 33.0 40.0 44 37.35440382915155 40.0 37.0 40.0 33.0 40.0 45 37.29335995853785 40.0 37.0 40.0 33.0 40.0 46 37.21532270357611 40.0 37.0 40.0 33.0 40.0 47 37.07905246791256 40.0 37.0 40.0 33.0 40.0 48 37.04175177586049 40.0 37.0 40.0 33.0 40.0 49 36.96188225968721 40.0 37.0 40.0 33.0 40.0 50 36.903539526233956 40.0 37.0 40.0 33.0 40.0 51 35.649541172525225 37.0 37.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1306 1 0.062121619302729414 1306 2 0.1586811314875618 1306 3 0.20738559208599128 1306 4 0.26989792149142744 1306 5 0.17177485426306305 1306 6 0.28654486626132325 1306 7 0.36343858288550734 1306 8 0.33044278549435546 1306 9 0.3158684660294 1306 10 0.2206887169680627 1306 11 0.2519158731903346 1306 12 0.23327488567974086 1306 13 0.2866130775446436 1306 14 0.516263939861787 1306 15 0.5169467529663478 1306 16 0.608138441672871 1306 17 0.4657114696696425 1306 18 0.4717458288643428 1306 19 0.6147738061221943 1306 20 0.622238548337414 1306 21 0.6189141601896608 1306 22 0.5215081487650082 1306 23 0.6328200753577349 1306 24 0.7524942874670373 1306 25 0.5279934762915346 1306 26 0.7032361904517117 1306 27 0.7509109491167223 1306 28 0.649489588255733 1306 29 0.6798178898834095 1306 30 0.7352146618391302 1306 31 0.7794457055507849 1306 32 0.7987947873990109 1306 33 0.6479482069863494 1306 34 0.855829690323965 1306 35 0.8073662775855723 1306 36 0.660567960332493 1306 37 0.6311028577910136 1306 38 0.8925289139148447 1306 39 0.7884770779713648 1306 40 0.8530219530398853 1306 41 0.6910574355223105 1306 42 0.7768076878083221 1306 43 0.7532162948737451 1306 44 0.9281515687460669 1306 45 0.9457177875187099 1306 46 0.9938656863779158 1306 47 0.8609836966371489 1306 48 0.8569741191802507 1306 49 0.906474820020513 1306 50 0.8330404138112755 1306 51 0.9267089192800668 1101 1 0.22292758478408103 1101 2 0.118853710864947 1101 3 0.24870721993117684 1101 4 0.3859399433451145 1101 5 0.22290794699178207 1101 6 0.32485144063471694 1101 7 0.3870469878097822 1101 8 0.36391489547133204 1101 9 0.2799639015532662 1101 10 0.28988133996425347 1101 11 0.347333407066877 1101 12 0.34636101345849823 1101 13 0.3701184690068757 1101 14 0.5474239899016382 1101 15 0.5375812738236689 1101 16 0.5237466971418243 1101 17 0.5322232378921186 1101 18 0.6005057371984179 1101 19 0.6900771450647127 1101 20 0.6759058082125406 1101 21 0.6495781016031756 1101 22 0.700992915393293 1101 23 0.6729266868181938 1101 24 0.6281696040908926 1101 25 0.7599941433050859 1101 26 0.6823985902612932 1101 27 0.7010596433200718 1101 28 0.6542700826760708 1101 29 0.7316612413358925 1101 30 0.7026763016407429 1101 31 0.875061475301024 1101 32 0.8849872609398801 1101 33 0.945684996796814 1101 34 0.8960850651800456 1101 35 1.0333112365130361 1101 36 1.018152956125597 1101 37 0.9422010222582884 1101 38 0.9014979628254807 1101 39 0.9674529354020649 1101 40 1.0240683755223898 1101 41 1.0424147804208985 1101 42 0.9113659089579116 1101 43 0.925166368494331 1101 44 1.0390135107795473 1101 45 0.784172502557233 1101 46 0.808885669107525 1101 47 0.8994663653556003 1101 48 1.0311757094955496 1101 49 0.9684735219733938 1101 50 1.0338606190632404 1101 51 1.0344253803995471 1305 1 0.4622332264455835 1305 2 0.4505338100589924 1305 3 0.4663141635145607 1305 4 0.24880417149142886 1305 5 0.5601677114059171 1305 6 0.32906718768989407 1305 7 -0.03477570282877451 1305 8 0.3830097497800722 1305 9 0.3455559660294014 1305 10 0.3591931812537794 1305 11 0.7943265874760499 1305 12 0.372895421394027 1305 13 0.7575952204017895 1305 14 1.222067511290355 1305 15 0.7343574672520603 1305 16 1.0887187988157265 1305 17 0.9831221839553592 1305 18 0.3204065431500567 1305 19 1.0362023775507652 1305 20 1.94868944119456 1305 21 1.223601660189658 1305 22 1.7661510059078651 1305 23 1.492641503929164 1305 24 1.4739228588956053 1305 25 1.5133729405772485 1305 26 1.4479906547374242 1305 27 1.6001296991167209 1305 28 1.6041770882557316 1305 29 0.9964473541691277 1305 30 2.013339661839133 1305 31 0.12062874126507239 1305 32 1.441093894541865 1305 33 1.7951580284149173 1305 34 1.2154279046096832 1305 35 1.953460027585571 1305 36 1.983559031761061 1305 37 1.337799286362447 1305 38 -0.6720916217994386 1305 39 0.3287672565427968 1305 40 -1.4575807255315425 1305 41 -1.4119559573348326 1305 42 -1.3111387407631057 1305 43 -1.5694399551262563 1305 44 -0.07028593125393456 1305 45 -2.017005426767007 1305 46 -0.669973599336366 1305 47 0.2237069109228642 1305 48 0.19280001203739516 1305 49 -1.2739939299794898 1305 50 0.04799577095413099 1305 51 -1.7400321521485012 1102 1 0.07761784183019671 1102 2 0.27301901715958365 1102 3 -0.05439589565703784 1102 4 -0.12634375750265292 1102 5 0.03354049247100477 1102 6 -0.07566654012075702 1102 7 0.3273544746860182 1102 8 0.09661921723569122 1102 9 0.0828340725382759 1102 10 0.04913400965614301 1102 11 0.20261061114469214 1102 12 0.29952264032893794 1102 13 0.3433940369698334 1102 14 0.48478940478148047 1102 15 0.5260734435834209 1102 16 0.42658862130092956 1102 17 0.4511695212334672 1102 18 0.5594597975879267 1102 19 0.29123196334958124 1102 20 0.4043107429697059 1102 21 -0.05864686052039758 1102 22 0.0306480473279791 1102 23 0.09855866369247934 1102 24 0.6005500778305191 1102 25 0.012781224600921348 1102 26 0.13615633521079928 1102 27 0.26521845651317477 1102 28 0.14500549062259438 1102 29 0.46212782754190584 1102 30 -0.30796211330879686 1102 31 0.04784767617631047 1102 32 0.020975551346602117 1102 33 -0.024960314780344106 1102 34 0.012469324728023423 1102 35 0.03274996841397382 1102 36 -0.2625948143927843 1102 37 0.24134958222042968 1102 38 0.14802672139582285 1102 39 -0.016794873634722762 1102 40 0.617567203462535 1102 41 0.26082510775392365 1102 42 0.048032856870030116 1102 43 0.23174347682640928 1102 44 0.3788264947815705 1102 45 0.46760995784838144 1102 46 0.5323932645689595 1102 47 0.20595543163292263 1102 48 0.7898414321557397 1102 49 0.2662427564110459 1102 50 0.7852738774630055 1102 51 0.6540506880881836 1304 1 -0.027663274293693974 1304 2 0.013372646924445064 1304 3 -0.030482287939356922 1304 4 0.04456563033814831 1304 5 -0.039142293522616 1304 6 0.054350578522814885 1304 7 0.007708279428491949 1304 8 0.012679537853010459 1304 9 0.0806964293118071 1304 10 -0.008672762560905767 1304 11 0.09989583341000952 1304 12 0.09127886151230769 1304 13 -0.1315127145415289 1304 14 0.01920896028000385 1304 15 0.1058705278730585 1304 16 0.16466754203899114 1304 17 -0.0360498219588834 1304 18 0.021934389379730135 1304 19 0.18903628587999322 1304 20 0.06648145696587449 1304 21 0.05026504116550967 1304 22 0.1735930956072238 1304 23 0.1123556488281281 1304 24 0.11630827042838376 1304 25 0.18089388685620378 1304 26 0.10116956060238635 1304 27 0.1316230455928178 1304 28 0.22783406213449098 1304 29 0.3506612526412809 1304 30 0.36016075597417085 1304 31 0.2116588053360431 1304 32 0.3849085815798148 1304 33 0.15365975340260007 1304 34 0.2256299745948951 1304 35 0.1296059122578228 1304 36 0.24546144674381054 1304 37 0.28146611731364857 1304 38 0.4065678163474331 1304 39 0.3640555759119408 1304 40 0.5309357850648055 1304 41 0.4567971230003067 1304 42 0.43834376293329314 1304 43 0.5603776890334302 1304 44 0.4658895246356707 1304 45 0.5719053667273286 1304 46 0.5716232464004491 1304 47 0.5248404742545532 1304 48 0.4248847828604596 1304 49 0.4240346555306189 1304 50 0.3745605812054862 1304 51 0.5352758813655427 1103 1 -0.07364463614983663 1103 2 -0.10213794566620038 1103 3 0.025092789468757815 1103 4 0.1281934844685253 1103 5 0.06856465797080347 1103 6 0.029830546468517127 1103 7 0.13316322846893058 1103 8 0.15705555130679016 1103 9 0.2539529125942863 1103 10 0.06606341026141394 1103 11 0.006540327934068557 1103 12 -0.15992900608689098 1103 13 0.040037968493393805 1103 14 0.0174873586186024 1103 15 0.10992998633603435 1103 16 0.1948256690447323 1103 17 0.1426641686881851 1103 18 -0.209364449216352 1103 19 -0.08517166825076572 1103 20 -0.013142619874145112 1103 21 0.19383066782324931 1103 22 0.1287464257551889 1103 23 0.10027509171542448 1103 24 0.0632358359948455 1103 25 0.07673171920320243 1103 26 -0.25811621549158303 1103 27 -0.11590083523442019 1103 28 0.03471143940077326 1103 29 -0.22416333285377732 1103 30 -0.027118353428043918 1103 31 0.01910202370781633 1103 32 -0.1497458001146228 1103 33 0.07760077650652164 1103 34 0.08642027102189331 1103 35 -0.07249417088770826 1103 36 0.21562010046335445 1103 37 -0.06296407241617885 1103 38 -0.14842749966203428 1103 39 0.16235504280233926 1103 40 0.34165591568982734 1103 41 0.2330822106040955 1103 42 0.2865711829010138 1103 43 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-0.18074696271193602 1209 13 -0.32849749482999613 1209 14 -0.47263447546460924 1209 15 -0.489483592350588 1209 16 -0.5079699429061293 1209 17 -0.9877387431969566 1209 18 -0.13919610585656272 1209 19 -0.5737313972836731 1209 20 -0.7864098965537849 1209 21 -0.5565307901414656 1209 22 -0.5199417093239234 1209 23 -0.5166300192496394 1209 24 -0.48899104838915974 1209 25 -0.5091436157141374 1209 26 -0.5142610008917146 1209 27 -0.9621219565124193 1209 28 -0.45476330909526297 1209 29 -0.41878443391034637 1209 30 -0.6469252388231226 1209 31 -0.8343381461521489 1209 32 -0.9529458405574758 1209 33 -0.6611333623135565 1209 34 -0.43821447949628123 1209 35 -0.8637585154607876 1209 36 -0.7442555377753592 1209 37 -0.5078960778759694 1209 38 -0.4773896350444744 1209 39 -0.8805042666360094 1209 40 -0.8661899970547253 1209 41 -0.7973864209109962 1209 42 -0.8985559593061581 1209 43 -0.6018902862520861 1209 44 -0.5530673882075732 1209 45 -0.5627007910054189 1209 46 -0.6050729370847137 1209 47 -0.3358957380837566 1209 48 -1.0913059482275074 1209 49 -0.656775386933127 1209 50 -0.8665737654697097 1209 51 -0.8930122845988322 1214 1 -0.055730845410707275 1214 2 -0.1123404414380147 1214 3 0.002242307227135143 1214 4 -0.06676469078401936 1214 5 -0.12246701913300484 1214 6 -0.3517711356633981 1214 7 -0.35453618187069225 1214 8 -0.2648944418366952 1214 9 -0.11073145912030213 1214 10 0.025660247122040403 1214 11 -0.11285904126646784 1214 12 -0.3079429019592652 1214 13 -0.21246465983772822 1214 14 -0.45936961445814717 1214 15 -0.5626485207718872 1214 16 -0.34960455447768624 1214 17 -0.06897362442787625 1214 18 -0.3310904628379632 1214 19 -0.4979293589761795 1214 20 -0.024364451021007483 1214 21 -0.2740031302295023 1214 22 -0.20630408391249944 1214 23 -0.6169393343941891 1214 24 -0.381166961463677 1214 25 -0.018363586368856488 1214 26 -0.061590183585927605 1214 27 -0.015439163158724512 1214 28 -0.4036073428819904 1214 29 -0.38618737636979716 1214 30 -0.5459417752866216 1214 31 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1215 16 -0.7659395862774403 1215 17 -0.6572504868520923 1215 18 -0.8466742021915579 1215 19 -0.790505696983395 1215 20 -0.7252236022836982 1215 21 -0.5267088988165511 1215 22 -0.3531036524772304 1215 23 -0.09058830973543053 1215 24 -0.6310460852037707 1215 25 -0.9363165004165381 1215 26 -0.6159845005420763 1215 27 -0.8744044623739597 1215 28 -1.1001707378312204 1215 29 -1.0575899129116237 1215 30 -0.7611944996515518 1215 31 -0.5948992090454865 1215 32 -0.8912042421040951 1215 33 -0.3004941454981278 1215 34 -0.6678019090549157 1215 35 -0.6918815873212623 1215 36 -0.754950285009123 1215 37 -0.4957410863083638 1215 38 -0.2565636714888839 1215 39 -0.892350755879562 1215 40 -0.4308726509973866 1215 41 -1.0840056467758288 1215 42 -0.32480333703639985 1215 43 -0.4290672843188048 1215 44 -0.5789815834278471 1215 45 -0.7244588429160714 1215 46 -0.7799114875351236 1215 47 -0.8166657598845859 1215 48 -0.6202434662234708 1215 49 -0.7758572840167517 1215 50 -0.8967247259402811 1215 51 -0.44251662419819127 1212 1 -0.10510201710742706 1212 2 0.09523295046013658 1212 3 0.007861441451524342 1212 4 -0.8554938227779161 1212 5 0.027216422007633412 1212 6 -0.3511620386711414 1212 7 -0.025606648387515918 1212 8 -0.21125959119414262 1212 9 -0.11977354686458597 1212 10 -0.2178841826430684 1212 11 -0.34034389962996414 1212 12 -0.321660452531475 1212 13 -0.531803060400506 1212 14 -0.21231644286437046 1212 15 -0.3779634496533788 1212 16 -0.04279982582610842 1212 17 -0.632637128365559 1212 18 -0.1254387290562491 1212 19 -0.25864002932602403 1212 20 -0.54128190551031 1212 21 -0.4546218355123415 1212 22 -0.2261126044073265 1212 23 -0.348619241056511 1212 24 -0.25559003508720224 1212 25 -0.14450671558321204 1212 26 -0.2531554770677289 1212 27 -0.5419906447228158 1212 28 -0.7299203329477066 1212 29 -0.603266112879588 1212 30 -0.4144540344359413 1212 31 -0.25272369426501484 1212 32 -0.09758805388220537 1212 33 -0.20656116929281865 1212 34 -0.11207925871410396 1212 35 -0.008144556941651615 1212 36 -0.3132891057747571 1212 37 -0.3392780775344022 1212 38 -0.30074491692838023 1212 39 -0.632264262081847 1212 40 -0.31517384874071297 1212 41 -0.5818699974494521 1212 42 -0.5727433252903325 1212 43 -0.37631674595719744 1212 44 -0.8126928080447655 1212 45 -0.5187246244747428 1212 46 -0.3226956623736186 1212 47 -0.4066655819138134 1212 48 -0.5639335123179023 1212 49 -0.09834923083908365 1212 50 -0.05057156428942022 1212 51 0.10695925186296051 1213 1 -0.08949091148544852 1213 2 0.048234959484283024 1213 3 -0.004950204301529482 1213 4 0.005126010571885331 1213 5 -0.40534952997339246 1213 6 -0.21346154794229477 1213 7 0.19051165349306132 1213 8 -0.021587951369355096 1213 9 0.03291228786847711 1213 10 0.25344605481699745 1213 11 -0.194179159650389 1213 12 0.5200218581756317 1213 13 0.4817331514362735 1213 14 0.01746981014093052 1213 15 0.11826551322907619 1213 16 2.1305168928620333E-4 1213 17 0.262432528782945 1213 18 0.633050221310981 1213 19 -0.04080911670210696 1213 20 -0.01682780018474972 1213 21 0.050038441798854194 1213 22 -0.48557313202316976 1213 23 -0.2636803351512924 1213 24 -1.2364219686905997 1213 25 -1.150994875514705 1213 26 -0.576147276297057 1213 27 -1.7194105307683358 1213 28 -0.37053555542242833 1213 29 -1.324242301003288 1213 30 -1.6521775795401794 1213 31 -1.4299459713786078 1213 32 -1.9956877146535348 1213 33 -1.9611638106655391 1213 34 -1.8179054287236553 1213 35 -2.400562960920176 1213 36 -2.8762110831814667 1213 37 -2.8564535872007752 1213 38 -1.7778387482362206 1213 39 -2.8689338928824952 1213 40 -2.111603714037294 1213 41 -2.7556341182543775 1213 42 -3.332977821222876 1213 43 -3.440704322942345 1213 44 -3.430056046196462 1213 45 -3.3767755417095344 1213 46 -2.8354908407156785 1213 47 -3.511925272985181 1213 48 -3.1784643557786936 1213 49 -2.22916634377259 1213 50 -2.8968318152527672 1213 51 -2.5009516923783863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 6.0 10 17.0 11 20.0 12 10.0 13 4.0 14 6.0 15 5.0 16 2.0 17 8.0 18 9.0 19 27.0 20 57.0 21 98.0 22 179.0 23 324.0 24 495.0 25 739.0 26 1079.0 27 1584.0 28 2254.0 29 3058.0 30 4034.0 31 5377.0 32 7153.0 33 9120.0 34 12416.0 35 17519.0 36 26396.0 37 48387.0 38 175156.0 39 12468.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.092750633888553 26.8198169885183 30.637183856666432 24.450248520926714 2 19.111307582085914 32.24048047315631 28.272918508582055 20.375293436175728 3 18.526874180665224 28.195176976311696 34.805036431816106 18.472912411206977 4 18.74973323984025 27.094295905612636 31.866101643242583 22.289869211304534 5 22.59534770281394 27.709825919941466 30.182616383646838 19.512209993597757 6 23.011798420779854 28.64150483216975 28.635712325843727 19.71098442120667 7 20.305173622755404 32.02920642663333 30.084143776104387 17.581476174506875 8 20.00365842504802 31.552391695375142 29.473186793085578 18.970763086491267 9 23.43678546385781 27.492454498338464 29.86341879820737 19.207341239596353 10 20.85157498079292 31.215777856367605 28.869465006524308 19.063182156315168 11 22.575531233803847 29.00429864943142 28.27108929605805 20.149080820706686 12 19.371055760495107 28.96984848022926 29.214658089692385 22.444437669583245 13 19.368311941709095 31.559708545471178 29.92439254900765 19.14758696381208 14 19.312520959726836 28.03634035547697 29.846346147983294 22.8047925368129 15 19.09118624432182 32.38803695009299 28.160117069601537 20.360659735983656 16 19.409164354745283 28.272613639828055 29.188744245602265 23.129477759824397 17 22.646565653486174 28.468339379896957 29.140574982470046 19.744519984146823 18 19.510380781073746 28.91802079204902 31.34477607390019 20.226822352977045 19 21.081369470442972 27.91103929758239 28.614371513063624 22.39321971891101 20 20.607908295478797 27.780250602115792 31.29782628578397 20.314014816621444 21 23.47428432059998 28.11713057528734 28.364074266028478 20.044510838084204 22 21.094783695619036 31.579525014481263 28.137861650559433 19.187829639340265 23 19.270007561528892 32.51396443642218 28.285301363514403 19.930726638534527 24 19.470138105545562 28.898814060546936 29.29209475320874 22.338953080698758 25 20.173721630751594 28.17473383821754 32.41289527921072 19.238649251820146 26 19.341512344738014 28.688634548192955 29.03650676536766 22.933346341701373 27 19.37837261059114 28.45187646718088 28.210115545257768 23.959635376970216 28 21.517636657418983 31.153623365141307 28.27383311484406 19.05490686259565 29 23.33434956251334 27.867443065760188 28.946068717417152 19.85213865430932 30 19.544214265026447 28.41267663602689 32.00469505038033 20.038414048566334 31 19.40751264454722 29.8457045124037 28.517988945357875 22.228793897691208 32 19.5222706624798 30.89722874302613 29.7176915337947 19.86280906069937 33 19.5216609249718 28.85308374744672 28.890582604188896 22.734672723392578 34 19.998353693671174 30.779830918242602 28.490245635001692 20.73156975308454 35 19.78018962836499 27.8689674095302 29.434468461327402 22.916374500777415 36 21.035944026096765 30.28444254748331 28.508886924179137 20.170726502240786 37 21.308081839716834 29.04511184212532 30.38502227086617 19.261784047291673 38 20.75485503490747 28.238468339379896 29.289350934422732 21.717325691289897 39 21.500868875948903 29.978964055973904 28.65857748239383 19.861589585683365 40 20.57510792419697 29.546230579741955 28.88923440891686 20.989427087144215 41 20.720237314902608 28.68449373938653 30.032957015637052 20.56231193007381 42 21.099630793808554 29.445641934018496 28.32430388931741 21.13042338285554 43 19.8109756097561 28.809756097560975 32.19115853658537 19.18810975609756 44 19.709081818070953 27.610702569543534 28.009792802351246 24.670422810034268 45 21.27539572205556 28.14199653666984 30.7715055730348 19.8111021682398 46 19.667388189384468 28.260418889668 30.327124173043508 21.74506874790403 47 20.803634035547695 29.232950214932472 29.05887015639767 20.90454559312216 48 19.8306113053844 27.98602451806843 31.860684201059136 20.32267997548803 49 21.697204353525805 29.506722356025733 29.329593609950916 19.466479680497546 50 20.71802907847041 27.334920688151847 30.100088717077885 21.846961516299856 51 30.024176165897888 23.816419671411452 26.002030432091804 20.157373730598856 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 43.0 1 46.5 2 50.0 3 57.0 4 64.0 5 67.5 6 71.0 7 69.5 8 68.0 9 83.0 10 98.0 11 129.5 12 161.0 13 219.5 14 278.0 15 410.0 16 542.0 17 619.5 18 697.0 19 926.5 20 1156.0 21 1479.0 22 1802.0 23 2454.5 24 3107.0 25 4128.0 26 6437.5 27 7726.0 28 9393.5 29 11061.0 30 13117.0 31 15173.0 32 17291.0 33 19409.0 34 21708.5 35 24008.0 36 25539.0 37 27070.0 38 28104.0 39 29138.0 40 29405.5 41 29673.0 42 29747.5 43 29822.0 44 27600.0 45 25378.0 46 23471.0 47 21564.0 48 21539.5 49 21515.0 50 19042.5 51 16570.0 52 16408.0 53 16246.0 54 11782.5 55 7319.0 56 6027.5 57 4736.0 58 3787.0 59 2838.0 60 2361.0 61 1884.0 62 1526.5 63 1169.0 64 958.0 65 747.0 66 686.5 67 626.0 68 522.5 69 419.0 70 335.5 71 252.0 72 200.5 73 149.0 74 126.0 75 81.5 76 60.0 77 48.0 78 36.0 79 23.5 80 11.0 81 13.0 82 15.0 83 10.0 84 5.0 85 3.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08261943233438004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0018292125240084143 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.01036553763604768 24 0.0 25 0.004268162556019634 26 0.0048779000640224385 27 0.0 28 0.0 29 0.0 30 0.001524343770007012 31 9.146062620042072E-4 32 0.0 33 0.0 34 9.146062620042072E-4 35 0.0 36 0.0 37 9.146062620042072E-4 38 0.0 39 0.0 40 6.097375080028048E-4 41 0.002134081278009817 42 0.0027438187860126216 43 0.003048687540014024 44 0.004268162556019634 45 6.097375080028048E-4 46 0.0 47 0.0 48 0.002134081278009817 49 0.0 50 3.048687540014024E-4 51 3.048687540014024E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 328010.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.79240195556162 #Duplication Level Percentage of deduplicated Percentage of total 1 95.41843342255444 83.77013461002895 2 2.8118567969879558 4.937193243252892 3 0.8543897691929428 2.250267901311191 4 0.4198371944744275 1.4743406293277694 5 0.19442056971838317 0.8534324402572794 6 0.10619131293410286 0.5593674257579753 7 0.03352499153428577 0.2060267672634372 8 0.05570729767940423 0.3912541975782705 9 0.026746522196825915 0.21133272848553822 >10 0.07258888166956093 0.9651435088281828 >50 0.0013469915756612165 0.0695773660220301 >100 0.002132221445941765 0.45106762491446434 >500 0.001059010798975668 0.5299466686826806 >1k 0.001765017237012306 3.330914888289372 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC 2955 0.900887168074144 TruSeq Adapter, Index 5 (100% over 51bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG 2613 0.7966220542056643 TruSeq Adapter, Index 15 (97% over 40bp) TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC 2088 0.6365659583549282 No Hit ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA 2072 0.6316880582909057 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC 1020 0.31096612908143045 TruSeq Adapter, Index 12 (100% over 51bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC 593 0.18078717112283163 TruSeq Adapter, Index 2 (100% over 51bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG 592 0.18048230236883023 TruSeq Adapter, Index 19 (97% over 40bp) TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC 525 0.16005609585073627 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG 392 0.11950855156854974 TruSeq Adapter, Index 13 (97% over 40bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.048687540014024E-4 0.0 0.0 2 3.048687540014024E-4 0.0 0.0 3 3.048687540014024E-4 0.0 0.0 4 6.097375080028048E-4 0.0 0.0 5 6.097375080028048E-4 0.0 0.0 6 0.001524343770007012 0.0 0.0 7 0.001524343770007012 0.0 0.0 8 0.001524343770007012 0.0 0.0 9 0.0018292125240084143 0.0 0.0 10 0.0018292125240084143 0.0 0.0 11 0.002134081278009817 0.0 0.0 12 0.002134081278009817 0.0 0.0 13 0.002134081278009817 0.0 0.0 14 0.002134081278009817 0.0 0.0 15 0.0024389500320112193 0.0 0.0 16 0.0027438187860126216 0.0 0.0 17 0.0027438187860126216 0.0 0.0 18 0.0027438187860126216 0.0 0.0 19 0.0027438187860126216 0.0 0.0 20 0.0027438187860126216 0.0 0.0 21 0.0027438187860126216 0.0 0.0 22 0.0033535562940154263 0.0 0.0 23 0.0033535562940154263 0.0 0.0 24 0.0033535562940154263 0.0 0.0 25 0.0033535562940154263 0.0 0.0 26 0.0033535562940154263 0.0 0.0 27 0.0033535562940154263 0.0 0.0 28 0.0033535562940154263 0.0 0.0 29 0.0033535562940154263 0.0 0.0 30 0.0036584250480168287 0.0 0.0 31 0.0036584250480168287 0.0 0.0 32 0.003963293802018231 0.0 0.0 33 0.003963293802018231 0.0 0.0 34 0.006097375080028048 0.0 0.0 35 0.006402243834029451 0.0 0.0 36 0.006402243834029451 0.0 0.0 37 0.007316850096033657 0.0 0.0 38 0.007316850096033657 0.0 0.0 39 0.007316850096033657 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 1075 0.0 43.557465 1 CTCGGGG 310 0.0 43.544403 16 GCACACG 1060 0.0 43.297924 11 AGCACAC 1085 0.0 42.92234 10 AGAGCAC 1085 0.0 42.71499 8 CACACGT 1070 0.0 42.683014 12 ACACGTC 1065 0.0 42.67215 13 GAGCACA 1095 0.0 42.324898 9 CACGTCT 1070 0.0 42.26249 14 CGTCTGA 1060 0.0 42.236702 16 TCGGAAG 1110 0.0 42.158306 3 TCGGGGA 365 0.0 41.913975 17 CCCAGGC 360 0.0 41.877556 34 GAACTCC 1065 0.0 41.833538 21 ATCGGAA 1120 0.0 41.78189 2 ACGTCTG 1080 0.0 41.662853 15 GTCTGAA 1080 0.0 41.45454 17 TGAACTC 1090 0.0 41.286922 20 TCCAGTC 1055 0.0 41.163647 25 CAGTCAC 1055 0.0 41.163647 27 >>END_MODULE