FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8115b-062415.3410000000d737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8115b-062415.3410000000d737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328010
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC29550.900887168074144TruSeq Adapter, Index 5 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG26130.7966220542056643TruSeq Adapter, Index 15 (97% over 40bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC20880.6365659583549282No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA20720.6316880582909057No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC10200.31096612908143045TruSeq Adapter, Index 12 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC5930.18078717112283163TruSeq Adapter, Index 2 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG5920.18048230236883023TruSeq Adapter, Index 19 (97% over 40bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC5250.16005609585073627No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG3920.11950855156854974TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10750.043.5574651
CTCGGGG3100.043.54440316
GCACACG10600.043.29792411
AGCACAC10850.042.9223410
AGAGCAC10850.042.714998
CACACGT10700.042.68301412
ACACGTC10650.042.6721513
GAGCACA10950.042.3248989
CACGTCT10700.042.2624914
CGTCTGA10600.042.23670216
TCGGAAG11100.042.1583063
TCGGGGA3650.041.91397517
CCCAGGC3600.041.87755634
GAACTCC10650.041.83353821
ATCGGAA11200.041.781892
ACGTCTG10800.041.66285315
GTCTGAA10800.041.4545417
TGAACTC10900.041.28692220
TCCAGTC10550.041.16364725
CAGTCAC10550.041.16364727
Produced by FastQC (version 0.11.2)