Basic Statistics
Measure | Value |
---|---|
Filename | C6001ACXX l05n01 ah8115a-062415.3410000000d6be.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 293444 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC | 2748 | 0.9364648791592264 | TruSeq Adapter, Index 5 (100% over 51bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC | 496 | 0.16902713976090838 | TruSeq Adapter, Index 12 (100% over 51bp) |
GCTTTCGCATGAAGTACCTCCCAACTACTTTTCCTCACACTTGTACTCCAT | 481 | 0.16391543190523575 | No Hit |
CATCTGCGTTATCGTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCT | 457 | 0.15573669933615952 | No Hit |
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA | 375 | 0.1277926963918158 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC | 368 | 0.12540723272583526 | TruSeq Adapter, Index 2 (100% over 51bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG | 317 | 0.10802742601654831 | TruSeq Adapter, Index 15 (97% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCAGGCG | 40 | 6.7830115E-9 | 45.001534 | 35 |
GAGCCTC | 20 | 7.023509E-4 | 45.001534 | 4 |
AGCACAC | 520 | 0.0 | 43.270706 | 10 |
ACACGTC | 520 | 0.0 | 43.263332 | 13 |
GATCGGA | 530 | 0.0 | 42.908077 | 1 |
GCACACG | 530 | 0.0 | 42.447044 | 11 |
CACACGT | 530 | 0.0 | 42.447044 | 12 |
CACGTCT | 530 | 0.0 | 42.02257 | 14 |
ACGTCTG | 540 | 0.0 | 41.244377 | 15 |
GAGCACA | 555 | 0.0 | 40.947342 | 9 |
CCCAGGC | 55 | 6.002665E-11 | 40.910484 | 34 |
GAACTCC | 540 | 0.0 | 40.834724 | 21 |
CGTCTGA | 550 | 0.0 | 40.49448 | 16 |
CACAGTG | 295 | 0.0 | 40.418217 | 33 |
ATCGGAA | 565 | 0.0 | 40.215755 | 2 |
TGAACTC | 550 | 0.0 | 40.092278 | 20 |
TCGGAAG | 570 | 0.0 | 39.862984 | 3 |
GTATGCC | 455 | 0.0 | 39.555046 | 45 |
CCGCCCC | 40 | 3.445566E-7 | 39.376343 | 28 |
CTCCAGT | 555 | 0.0 | 39.325665 | 24 |