FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8115a-062415.3410000000d6be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8115a-062415.3410000000d6be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293444
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC27480.9364648791592264TruSeq Adapter, Index 5 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC4960.16902713976090838TruSeq Adapter, Index 12 (100% over 51bp)
GCTTTCGCATGAAGTACCTCCCAACTACTTTTCCTCACACTTGTACTCCAT4810.16391543190523575No Hit
CATCTGCGTTATCGTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCT4570.15573669933615952No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA3750.1277926963918158No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC3680.12540723272583526TruSeq Adapter, Index 2 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG3170.10802742601654831TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGCG406.7830115E-945.00153435
GAGCCTC207.023509E-445.0015344
AGCACAC5200.043.27070610
ACACGTC5200.043.26333213
GATCGGA5300.042.9080771
GCACACG5300.042.44704411
CACACGT5300.042.44704412
CACGTCT5300.042.0225714
ACGTCTG5400.041.24437715
GAGCACA5550.040.9473429
CCCAGGC556.002665E-1140.91048434
GAACTCC5400.040.83472421
CGTCTGA5500.040.4944816
CACAGTG2950.040.41821733
ATCGGAA5650.040.2157552
TGAACTC5500.040.09227820
TCGGAAG5700.039.8629843
GTATGCC4550.039.55504645
CCGCCCC403.445566E-739.37634328
CTCCAGT5550.039.32566524