FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8104b-062415.3410000000d81c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8104b-062415.3410000000d81c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532339
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA17030.3199089302117636No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG15140.2844052380156254TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC12290.23086792438652814TruSeq Adapter, Index 5 (100% over 51bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC8330.15647923597557195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGC207.031098E-444.998414
GCACACG4350.041.3778411
AGCACAC4650.038.7046710
CACGTCT4700.038.29651314
GAACTCC4750.037.90051321
ACACGTC4750.037.8933913
CACACGT4750.037.8933912
CGTCTGA4800.037.4986716
ACGTCTG4950.037.27140415
TCCAGTC4800.037.02993825
CCAGTCA4800.037.02993826
GAGCACA4950.036.3589329
GATCGGA5050.036.1013951
TCGGAAG5050.036.084443
CGCGCCG250.002105489836.00210618
CAGTCAC5000.035.5487427
CCCTGCC702.1827873E-1135.3558924
ACTCCAG5250.034.2909423
AGAGCAC5300.033.9578678
TGAACTC5350.033.64998620
Produced by FastQC (version 0.11.2)