FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l05n01 ah8104a-062415.3410000000d7bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l05n01 ah8104a-062415.3410000000d7bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342615
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC98522.875530843658334TruSeq Adapter, Index 5 (100% over 51bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC48761.423171781737519No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC39831.1625293697006844TruSeq Adapter, Index 2 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC12300.3590035462545423TruSeq Adapter, Index 12 (100% over 51bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC10220.2982940034732864No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA4170.12171095836434481No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3490.10186360783970345TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGGG4250.044.998115
TCGGGGA5950.044.6264817
GATCGGA18900.044.0843621
CTCGGGG4400.043.9754216
ACACGTC18950.043.92954313
GCACACG18950.043.92954311
CACGTCT18900.043.9267214
CACACGT19050.043.8170512
GAACTCC18800.043.80773521
CGTCTGA18850.043.8045216
ACGTCTG19000.043.69552615
GTATGCC17900.043.61548245
CAGGCGG6150.043.5347536
CCAGGCG6100.043.52275535
GTCTCCC6100.043.52275542
TCGGAAG19300.043.4826243
TCCTCGG4450.043.4813114
CAGTCAC18900.043.33150527
CGTATGC18050.043.25302544
ATCGGAA19250.043.2449262