FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 n_60_3.3410000000d935.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 n_60_3.3410000000d935.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097174
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGC803.6379788E-1233.74620414
TTACGCA855.456968E-1231.76113515
TCGCTAG2250.030.99651533
CGCTAGC2550.027.34986734
CATTCGC2650.027.16799430
ATTCGCT2700.025.83160631
TCTTACG1058.54925E-1125.71139313
TACGCAC1101.5097612E-1024.54940616
TTCGCTA2850.024.47093232
CCGGATT3050.024.3726271
CGGATTG3250.024.2280462
TAGCCGC2900.024.0490210
CTAGCGC851.8955507E-723.820858
AAAGCCG2750.023.73109416
CTTAGAA2750.023.72460610
ATAACCG1152.6375346E-1023.48418244
CTAGCCA3000.023.24738736
TATCTTA2850.022.8921627
CCGTATA802.9703933E-622.50362218
TTTGCCG3050.022.1296728