FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 n_60_2.3410000000d90e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 n_60_2.3410000000d90e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981950
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG950.033.15549537
ACGCTCG2300.028.37040143
CTCACGC2400.027.18553440
TATCGAT2550.026.47406421
TAGTCCG856.4846972E-926.46867231
CCCGCTA1200.026.2494352
CGTAAGC608.194565E-626.248112
GTGATAC2600.025.09433629
ATAGTCG551.366786E-424.54867616
TCTCACG2750.024.54367839
TAGCGTC751.6982976E-623.9982632
GTAAGCC751.6982976E-623.9982613
GGGTCAC1305.0931703E-1122.50295420
ATATCGA3150.022.14576320
TCACGCT2950.022.11704441
GCCCGCT1650.021.8432961
TGTCCTA1551.8189894E-1221.77261736
CGCTACA2000.021.3734514
CTACGCT855.034133E-621.1749368
TAAGCCG754.604326E-520.99847814
Produced by FastQC (version 0.11.2)