FastQCFastQC Report
Tue 30 Jun 2015
C6001ACXX l02n01 n_60_1.3410000000d8c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC6001ACXX l02n01 n_60_1.3410000000d8c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959937
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCTCCTGCGCCTCCGCCGCCGCCGCCTTCTTCGTCTGCTGAGTTCGT17370.18094937480272144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGACT803.6379788E-1233.74851225
CGCTTAG651.4037141E-831.15247542
CGTCTAG300.00514794929.9986834
GTCTACT4250.028.58846721
CGGATAG1700.027.821881
GCGGCAT6050.027.548151
TGCGCCT4350.027.41258610
TCCTGCG4550.026.7021227
TCGTCTA3050.026.55897719
AGTTCGT4400.026.07975845
TCGTCTG4400.026.078435
TCTACTG4900.025.71449522
TAACCCG709.3042036E-725.71449514
GCTGAGT4650.025.64403541
CTGAGTT4850.024.58654842
TGCACCG5200.024.22970427
ACTGCAC5300.024.19704825
CCTGCCT4800.023.9301281
TTCGTCT4950.023.63532334
CGGCATG5750.023.478452
Produced by FastQC (version 0.11.2)